Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 2 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 12 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 22 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
elastase-2 |
23-38 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
matrix metallopeptidase-2 |
23-38 |
N |
|
MS |
Schilling & Overall, 2008 |
| 38 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 40 |
meprin alpha subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 40 |
meprin beta subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 45 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 49 |
caspase-3 |
1-353 |
P |
normal turnover |
|
Thiede et al., 2001 |
| 55 |
caspase-3 |
1-353 |
P |
normal turnover |
|
Thiede et al., 2001 |
| 58 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 68 |
cathepsin B |
63-84 |
N |
|
MS |
Biniossek et al., 2011 |
| 74 |
cathepsin B |
63-84 |
N |
|
MS |
Biniossek et al., 2011 |
| 74 |
glutamyl endopeptidase I |
62-89 |
N |
|
MS |
Schilling & Overall, 2008 |
| 76 |
caspase-3 |
62-89 |
N |
|
MS |
Schilling & Overall, 2008 |
| 78 |
LAST_MAM peptidase (Limulus-type) |
75-89 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 89 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 95 |
matrix metallopeptidase-2 |
69-115 |
N |
|
MS |
Schilling & Overall, 2008 |
| 107 |
matrix metallopeptidase-2 |
102-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 112 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 115 |
meprin alpha subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 115 |
meprin beta subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 115 |
chymotrypsin A (cattle-type) |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 116 |
elastase-2 |
115-129 |
N |
|
MS |
Schilling & Overall, 2008 |
| 117 |
meprin alpha subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 117 |
meprin beta subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 121 |
glutamyl endopeptidase I |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 129 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 131 |
meprin alpha subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 131 |
meprin beta subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 132 |
meprin beta subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 133 |
meprin alpha subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 133 |
meprin beta subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 134 |
meprin alpha subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 134 |
meprin beta subunit |
1-353 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 136 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 137 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 147 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 150 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 153 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 156 |
cathepsin L |
154-168 |
N |
|
MS |
Biniossek et al., 2011 |
| 156 |
cathepsin S |
154-168 |
N |
|
MS |
Biniossek et al., 2011 |
| 156 |
cathepsin B |
154-168 |
N |
|
MS |
Biniossek et al., 2011 |
| 157 |
HIV-1 retropepsin |
154-168 |
N |
|
MS |
Schilling & Overall, 2008 |
| 158 |
cathepsin L |
154-168 |
N |
|
MS |
Biniossek et al., 2011 |
| 158 |
elastase-2 |
154-168 |
N |
|
MS |
Schilling & Overall, 2008 |
| 159 |
cathepsin B |
154-168 |
N |
|
MS |
Biniossek et al., 2011 |
| 160 |
LAST_MAM peptidase (Limulus-type) |
157-173 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 163 |
meprin alpha subunit |
160-173 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 163 |
meprin beta subunit |
160-173 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 163 |
LAST_MAM peptidase (Limulus-type) |
160-173 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 164 |
matrix metallopeptidase-2 |
161-173 |
N |
|
MS |
Schilling & Overall, 2008 |
| 168 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 172 |
peptidyl-Lys metallopeptidase |
1-353 |
N |
|
MS |
|
| 174 |
chymotrypsin A (cattle-type) |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 175 |
cathepsin L |
174-185 |
N |
|
MS |
Biniossek et al., 2011 |
| 203 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 204 |
cathepsin B |
204-213 |
N |
|
MS |
Biniossek et al., 2011 |
| 208 |
cathepsin B |
204-224 |
N |
|
MS |
Biniossek et al., 2011 |
| 210 |
cathepsin L |
204-228 |
N |
|
MS |
Biniossek et al., 2011 |
| 213 |
granzyme A |
1-353 |
N |
|
|
Van Damme et al., 2010 |
| 213 |
trypsin 1 |
1-353 |
N |
|
MS |
Schilling & Overall, 2008 |
| 218 |
meprin alpha subunit |
215-228 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 332 |
cathepsin L |
326-350 |
N |
|
MS |
Biniossek et al., 2011 |
| 332 |
cathepsin S |
326-350 |
N |
|
MS |
Biniossek et al., 2011 |
| 332 |
cathepsin B |
326-348 |
N |
|
MS |
Biniossek et al., 2011 |
| 336 |
astacin |
333-350 |
N |
|
MS |
Becker-Pauly et al., 2011 |
