Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-417 |
P |
|
NT |
<%Agarwal et al., 2012[]%> |
| 10 |
granzyme B (Homo sapiens-type) |
2-417 |
N |
|
MS |
Van Damme et al., 2010 |
| 22 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 30 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 41 |
lysyl endopeptidase (bacteria) |
2-417 |
N |
|
MS |
|
| 48 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 56 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 63 |
matrix metallopeptidase-2 |
57-75 |
N |
|
MS |
Schilling & Overall, 2008 |
| 64 |
cathepsin S |
57-75 |
N |
|
MS |
Biniossek et al., 2011 |
| 65 |
cathepsin L |
57-75 |
N |
|
MS |
Biniossek et al., 2011 |
| 65 |
cathepsin S |
57-76 |
N |
|
MS |
Biniossek et al., 2011 |
| 75 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 86 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 87 |
matrix metallopeptidase-2 |
84-98 |
N |
|
MS |
Schilling & Overall, 2008 |
| 97 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 98 |
glutamyl endopeptidase I |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 103 |
granzyme B (Homo sapiens-type) |
2-417 |
N |
|
MS |
Van Damme et al., 2010 |
| 106 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
LAST_MAM peptidase (Limulus-type) |
108-123 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 123 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 151 |
thrombin |
148-164 |
N |
|
MS |
Schilling & Overall, 2008 |
| 156 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 164 |
glutamyl endopeptidase I |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 171 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 175 |
cathepsin B |
172-184 |
N |
|
MS |
Biniossek et al., 2011 |
| 175 |
matrix metallopeptidase-2 |
171-184 |
N |
|
MS |
Schilling & Overall, 2008 |
| 184 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 188 |
chymotrypsin A (cattle-type) |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 191 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 193 |
glutamyl endopeptidase I |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 201 |
meprin beta subunit |
198-216 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 202 |
meprin beta subunit |
199-216 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 202 |
glutamyl endopeptidase I |
199-216 |
N |
|
MS |
Schilling & Overall, 2008 |
| 203 |
HIV-1 retropepsin |
199-216 |
N |
|
MS |
Schilling & Overall, 2008 |
| 210 |
matrix metallopeptidase-2 |
205-233 |
N |
|
MS |
Schilling & Overall, 2008 |
| 216 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 233 |
glutamyl endopeptidase I |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 245 |
peptidyl-Lys metallopeptidase |
2-417 |
N |
|
MS |
|
| 246 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 252 |
cathepsin S |
247-264 |
N |
|
MS |
Biniossek et al., 2011 |
| 254 |
cathepsin S |
247-264 |
N |
|
MS |
Biniossek et al., 2011 |
| 263 |
peptidyl-Lys metallopeptidase |
2-417 |
N |
|
MS |
|
| 264 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 279 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 281 |
cathepsin K |
279-297 |
N |
|
MS |
Schilling & Overall, 2008 |
| 285 |
cathepsin B |
280-297 |
N |
|
MS |
Biniossek et al., 2011 |
| 285 |
matrix metallopeptidase-2 |
279-297 |
N |
|
MS |
Schilling & Overall, 2008 |
| 286 |
HIV-1 retropepsin |
279-297 |
N |
|
MS |
Schilling & Overall, 2008 |
| 287 |
elastase-2 |
279-297 |
N |
|
MS |
Schilling & Overall, 2008 |
| 288 |
cathepsin L |
280-297 |
N |
|
MS |
Biniossek et al., 2011 |
| 288 |
cathepsin S |
280-297 |
N |
|
MS |
Biniossek et al., 2011 |
| 289 |
cathepsin S |
280-297 |
N |
|
MS |
Biniossek et al., 2011 |
| 291 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 297 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 309 |
matrix metallopeptidase-2 |
297-322 |
N |
|
MS |
Schilling & Overall, 2008 |
| 309 |
elastase-2 |
297-322 |
N |
|
MS |
Schilling & Overall, 2008 |
| 310 |
cathepsin B |
298-323 |
N |
|
MS |
Biniossek et al., 2011 |
| 310 |
matrix metallopeptidase-2 |
297-323 |
N |
|
MS |
Schilling & Overall, 2008 |
| 312 |
cathepsin L |
298-323 |
N |
|
MS |
Biniossek et al., 2011 |
| 312 |
cathepsin S |
298-323 |
N |
|
MS |
Biniossek et al., 2011 |
| 312 |
cathepsin B |
298-323 |
N |
|
MS |
Biniossek et al., 2011 |
| 315 |
granzyme B (Homo sapiens-type) |
2-417 |
N |
|
MS |
Van Damme et al., 2010 |
| 322 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 323 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 330 |
thrombin |
326-344 |
N |
|
MS |
Schilling & Overall, 2008 |
| 341 |
matrix metallopeptidase-2 |
332-350 |
N |
|
MS |
Schilling & Overall, 2008 |
| 344 |
glutamyl endopeptidase I |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 350 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 353 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 361 |
cathepsin L |
354-379 |
N |
|
MS |
Biniossek et al., 2011 |
| 361 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 369 |
cathepsin L |
366-382 |
N |
|
MS |
Biniossek et al., 2011 |
| 369 |
cathepsin S |
366-382 |
N |
|
MS |
Biniossek et al., 2011 |
| 369 |
cathepsin B |
366-382 |
N |
|
MS |
Biniossek et al., 2011 |
| 372 |
cathepsin S |
366-382 |
N |
|
MS |
Biniossek et al., 2011 |
| 388 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
| 391 |
matrix metallopeptidase-2 |
388-406 |
N |
|
MS |
Schilling & Overall, 2008 |
| 394 |
elastase-2 |
388-406 |
N |
|
MS |
Schilling & Overall, 2008 |
| 395 |
cathepsin L |
389-406 |
N |
|
MS |
Biniossek et al., 2011 |
| 406 |
trypsin 1 |
2-417 |
N |
|
MS |
Schilling & Overall, 2008 |
