$VAR1 = undef;

Summary for peptidase S07.001: flavivirin

Names
MEROPS Name	flavivirin
Other names	dengue virus NS3 protease, encephalitis virus proteinase, Langat virus NS3 peptidase, Murray Valley encephalitis virus proteinase, NS3 protein, NS2B/NS3 peptidase, NS2B-3 proteinase, tick-borne encephalitis virus peptidase, St. Louis encephalitis virus peptidase, West Nile flavivirus proteinase, yellow fever virus (flavivirus) protease

Domain architecture

MEROPS Classification
Classification	Clan PA >> Subclan PA(S) >> Family S7 >> Subfamily (none) >> S07.001
Holotype	flavivirin (yellow fever virus), Uniprot accession Q9YRV3 (peptidase unit: 1485-1666), MERNUM MER0000290
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Serine
Peplist	Included in the Peplist with identifier PL00340
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.91
Enzymology	BRENDA database
Proteolytic events	CutDB database (5 cleavages)
Physiology	Contributes to processing of viral polyprotein.
Pharmaceutical relevance	Potential drug target.
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/gk/KR/RGS/g/-/- (based on 29 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	3	6	2	0	14	6	2	1
Pro	1	1	0	0	0	0	3	0
Ala	3	4	0	1	1	3	4	5
Val	0	1	0	0	0	0	3	2
Leu	4	2	0	0	0	3	2	5
Ile	0	0	0	0	0	1	1	0
Met	0	0	0	0	1	0	0	0
Phe	1	0	0	0	0	0	0	1
Tyr	1	1	1	0	0	0	1	0
Trp	0	0	0	0	0	2	0	1
Ser	2	1	0	0	8	3	2	3
Thr	2	3	0	0	0	2	2	0
Cys	1	0	0	0	0	0	0	0
Asn	3	0	0	0	0	0	0	2
Gln	1	0	1	0	0	2	0	0
Asp	0	0	0	0	0	0	1	1
Glu	1	0	0	0	0	0	1	1
Lys	1	6	14	2	0	1	1	2
Arg	3	3	11	26	0	1	1	0
His	1	0	0	0	0	0	0	0

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Dengue virus 3	recombinant	n/L/K	K/G/R	R	R	-	-	-	-	nKRR	ACC	Li et al., 2005
dengue virus 4	recombinant	-	-	-	-	S	G/D	S	G/N	xxxxSGSG	AMCA-Dnp	Li et al., 2005
dengue virus 3	recombinant	-	-	-	-	S	D/G/P	S	G	xxxxSDSG	AMCA-Dnp	Li et al., 2005
dengue virus 2	recombinant	-	-	-	-	S	G	S	G/N	xxxxSGSG	AMCA-Dnp	Li et al., 2005
dengue virus 1	recombinant	-	-	-	-	S	D/E	S	N/D/G	xxxxSDSN	AMCA-Dnp	Li et al., 2005
Yellow fever virus	recombinant	-	G/E	K/R	K/R	T/S	L/A	R/I	-	xGKKTLRx	Dnp-Abz	Alves et al., 2007
Yellow fever virus	recombinant	R	R	R	R/K	S	A	G	-	RRRRSAGx	Abz-Tyr(NO2)	Nigomrattanakit et al., 2006
Dengue virus 1	recombinant	n/L	K	R/T	R	-	-	-	-	nKRR	ACC	Li et al., 2005
Dengue virus 4	recombinant	L/K/n	K	R	R	-	-	-	-	LKRR	ACC	Li et al., 2005
Dengue virus 2	recombinant	n/L/K	K/R	R/T	R	-	-	-	-	nKRR	ACC	Li et al., 2005