$VAR1 = undef;

Summary for peptidase C01.075: cruzipain

Names
MEROPS Name	cruzipain
Other names	congopain (Trypanosoma congolense), cruzain, evansain (Trypanosoma evansi), GP57/51

Domain architecture

MEROPS Classification
Classification	Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.075
Holotype	cruzipain (Trypanosoma cruzi), Uniprot accession P25779 (peptidase unit: 122-337), MERNUM MER0001691
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Cysteine
Peplist	Included in the Peplist with identifier PL00071
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.51
Enzymology	BRENDA database
Proteolytic events	CutDB database (4 cleavages)
Physiology	Nutrition of parasite by digestion of host proteins. Immune avoidance. Antigen.
Pharmaceutical relevance	Potential drug target for Chagas disease.
Pathways	KEGG	Chagas disease (American trypanosomiasis)
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/Cruzipain
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	K/K/L/krA/ks/K/Rq (based on 65 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	0	1	0	5	1	0	2	1
Pro	0	4	1	0	0	4	6	3
Ala	3	2	2	2	30	1	3	2
Val	1	3	5	1	1	2	1	0
Leu	0	3	39	3	5	3	1	4
Ile	3	0	0	1	0	2	0	2
Met	0	0	0	4	2	0	0	0
Phe	0	1	9	4	7	0	0	0
Tyr	0	0	3	3	2	1	1	1
Trp	0	0	1	1	0	1	0	0
Ser	5	4	0	1	4	9	2	1
Thr	0	0	0	1	0	1	0	0
Cys	1	1	2	0	0	0	1	0
Asn	0	0	0	1	0	0	1	1
Gln	1	0	0	1	2	0	1	8
Asp	0	0	0	0	0	0	0	0
Glu	2	1	0	1	0	1	1	1
Lys	25	30	2	17	3	26	29	3
Arg	1	0	1	12	0	3	4	25
His	1	0	0	1	0	1	0	0

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Trypanosoma cruzi	recombinant	H/K	R	F	R	-	-	-	-	HRFR	ACC	Choe et al., 2006
Trypanosoma cruzi	recombinant	-	-	-	Amf	-	-	-	-	xxxAmfxxxx	Abz-EDDnp	Alves et al., 2001