Literature for peptidase C01.075: cruzipain

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates Pharma

(Topics flags: S Structure, T Target, P Specificity, I Inhibitor, E Expression, U Therapeutic, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2025

Cerutti,J.P., Diniz,L.A., Correa Santos,V., Vilchez Larrea,S.C., Alonso,G.D., Ferreira,R.S., Quevedo,M.A. and Dehaen,W. Discovery of a Potent Triazole-Based Reversible Targeted Covalent Inhibitor of Cruzipain ACS Med Chem Lett (2025) 16, 72-79. PubMed Europe PubMed DOI PMC EPMC I
Cerutti,J.P., Estrada Canepa,J.I., Diniz,L.A., Vilchez Larrea,S.C., Santos,V.C., Alonso,G.D., Ferreira,R.S., Dehaen,W. and Quevedo,M.A. Structure-Based Discovery of Non-Covalent Triazole-Based Cruzipain Inhibitors with Improved Bioactivity Translation J Chem Inf Model (2025) PubMed Europe PubMed DOI I
Sheu-Idrees,R., Marques,G.V.L., Santana,P.A.L., Diniz,L.A., Resende,D.M., Odoma,S., Olorunshola,O., Ferreira,R.S., Murta,S.M.F., Maltarollo,V.G. and de Oliveira,R.B. Investigation of the activity of 4-aminoquinolines as cysteine protease inhibitors with application in the treatment of Chagas disease Mem Inst Oswaldo Cruz (2025) 120, e240161-e240161. PubMed Europe PubMed DOI PMC EPMC I

2024

Cerutti,J.P., Diniz,L.A., Santos,V.C., Vilchez Larrea,S.C., Alonso,G.D., Ferreira,R.S., Dehaen,W. and Quevedo,M.A. Structure-Aided Computational Design of Triazole-Based Targeted Covalent Inhibitors of Cruzipain Molecules (2024) 29 PubMed Europe PubMed DOI PMC EPMC
Prates,J.L.B., Lopes,J.R., Chin,C.M., Ferreira,E.I., Dos Santos,J.L. and Scarim,C.B. Discovery of Novel Inhibitors of Cruzain Cysteine Protease of Trypanosoma cruz Curr Med Chem (2024) 31, 2285-2308. PubMed Europe PubMed DOI V

2023

Santos,V.C., Leite,P.G., Santos,L.H., Pascutti,P.G., Kolb,P., Machado,F.S. and Ferreira,R.S. Structure-based discovery of novel cruzain inhibitors with distinct trypanocidal activity profiles Eur J Med Chem (2023) 257, 115498-115498. PubMed Europe PubMed DOI I

2022

de Oliveira,A.S., Valli,M., Ferreira,L.L., Souza,J.M., Krogh,R., Meier,L., Abreu,H.R., Voltolini,B.G., Llanes,L.C., Nunes,R.J., Braga,A.L. and Andricopulo,A.D. Novel trypanocidal thiophen-chalcone cruzain inhibitors: structure- and ligand-based studies Future Med Chem (2022) 14, 795-808. PubMed Europe PubMed DOI I
Reis,C.R.C.D., Souza,H.V.C.M., Leme,R.P.P., Castelo-Branco,F.S., Fernandes,T.V.A., Boechat,N., Dias,L.R.S. and Hoelz,L.V.B. Study of the dynamic behavior of the cruzain enzyme in free and complexed forms with competitive and noncovalent benzimidazole inhibitors J Biomol Struct Dyn (2022) , 1-15. PubMed Europe PubMed DOI
Santos,V.C. and Ferreira,R.S. Computational approaches towards the discovery and optimisation of cruzain inhibitors Mem Inst Oswaldo Cruz (2022) 117, e210385-e210385. PubMed Europe PubMed DOI PMC EPMC V

2021

Barbosa da Silva,E., Rocha,D.A., Fortes,I.S., Yang,W., Monti,L., Siqueira-Neto,J.L., Caffrey,C.R., McKerrow,J., Andrade,S.F. and Ferreira,R.S. Structure-Based Optimization of Quinazolines as Cruzain and TbrCATL Inhibitors J Med Chem (2021) 64, 13054-13071. PubMed Europe PubMed DOI I
Santos Nascimento,I.J.D., de Aquino,T.M. and da Silva-Junior,E.F. Cruzain and Rhodesain Inhibitors: Last Decade of Advances in Seeking for New Compounds Against American and African Trypanosomiases Curr Top Med Chem (2021) PubMed Europe PubMed DOI V
Valois,M.V., de Oliveira,C., Lapa,A.J., Souccar,C. and Oliva,M.L.V. Bauhinia Protease Inhibitors Attenuate Gastric Ulcer by Blocking Neutrophil Enzymes Planta Med (2021) 87, 169-176. PubMed Europe PubMed DOI

2020

Alvarez,V.E., Iribarren,P.A., Niemirowicz,G.T. and Cazzulo,J.J. Update on relevant trypanosome peptidases: Validated targets and future challenges Biochim Biophys Acta Proteins Proteom (2020) 1869, 140577-140577. PubMed Europe PubMed DOI
Arafet,K., Gonzalez,F.V. and Moliner,V. Quantum mechanics/molecular mechanics studies of the mechanism of cysteine proteases inhibition by dipeptidyl nitroalkenes Chemistry (2020) 26, 2002-2012. PubMed Europe PubMed DOI I
Bonatto,V., Batista,P.H.J., Cianni,L., De Vita,D., Silva,D.G., Cedron,R., Tezuka,D.Y., de Albuquerque,S., Moraes,C.B., Franco,C.H., Lameira,J., Leitao,A. and Montanari,C.A. On the intrinsic reactivity of highly potent trypanocidal cruzain inhibitors RSC Med Chem (2020) 11, 1275-1284. PubMed Europe PubMed DOI PMC EPMC I
Cerny,N., Bivona,A.E., Sanchez Alberti,A., Trinitario,S.N., Morales,C., Cardoso Landaburu,A., Cazorla,S.I. and Malchiodi,E.L. Cruzipain and Its Physiological Inhibitor, Chagasin, as a DNA-Based Therapeutic Vaccine Against Trypanosoma cruzi Front Immunol (2020) 11, 565142-565142. PubMed Europe PubMed DOI PMC EPMC
Chenna,B.C., Li,L., Mellott,D.M., Zhai,X., Siqueira-Neto,J.L., Calvet Alvarez,C., Bernatchez,J.A., Desormeaux,E., Alvarez Hernandez,E., Gomez,J., McKerrow,J.H., Cruz-Reyes,J. and Meek,T.D. Peptidomimetic vinyl heterocyclic inhibitors of cruzain effect anti-trypanosomal activity J Med Chem (2020) 63, 3298-3316. PubMed Europe PubMed DOI I
Cianni,L., Lemke,C., Gilberg,E., Feldmann,C., Rosini,F., Rocho,F.D.R., Ribeiro,J.F.R., Tezuka,D.Y., Lopes,C.D., de Albuquerque,S., Bajorath,J., Laufer,S., Leitao,A., Gutschow,M. and Montanari,C.A. Mapping the S1 and S1' subsites of cysteine proteases with new dipeptidyl nitrile inhibitors as trypanocidal agents PLoS Negl Trop Dis (2020) 14, e0007755-e0007755. PubMed Europe PubMed DOI I
Cianni,L., Rocho,F.D.R., Rosini,F., Bonatto,V., Ribeiro,J.F.R., Lameira,J., Leitao,A., Shamim,A. and Montanari,C.A. Optimization strategy of single-digit nanomolar cross-class inhibitors of mammalian and protozoa cysteine proteases Bioorg Chem (2020) 101, 104039-104039. PubMed Europe PubMed DOI I
de Souza,M.L., de Oliveira Rezende Junior,C., Ferreira,R.S., Espinoza Chavez,R.M., Ferreira,L.L.G., Slafer,B.W., Magalhaes,L.G., Krogh,R., Oliva,G., Cruz,F.C., Dias,L.C. and Andricopulo,A.D. Discovery of potent, reversible and competitive cruzain inhibitors with trypanocidal activity: a structure-based drug design approach J Chem Inf Model (2020) 60, 1028-1041. PubMed Europe PubMed DOI I
Fonseca Lameiro,R.D., Shamim,A., Rosini,F., Cendron,R., Jatai Batista,P.H. and Montanari,C.A. Synthesis, biochemical evaluation and molecular modeling studies of nonpeptidic nitrile-based fluorinated compounds Future Med Chem (2020) PubMed Europe PubMed DOI
Ribeiro,J.F.R., Cianni,L., Li,C., Warwick,T.G., De Vita,D., Rosini,F., Dos Reis Rocho,F., Martins,F.C.P., Kenny,P.W., Lameira,J., Leitao,A., Emsley,J. and Montanari,C.A. Crystal structure of Leishmania mexicana cysteine protease B in complex with a high-affinity azadipeptide nitrile inhibitor Bioorg Med Chem (2020) 28, 115743-115743. PubMed Europe PubMed DOI I
Silva,J.R.A., Cianni,L., Araujo,D., Batista,P.H.J., De Vita,D., Rosini,F., Leitao,A., Lameira,J. and Montanari,C.A. Assessment of the cruzain cysteine protease reversible and irreversible covalent inhibition mechanism J Chem Inf Model (2020) 60, 1666-1677. PubMed Europe PubMed DOI I
Toman,N.P., Kamenik,A.S., Santos,L.H., Hofer,F., Liedl,K.R. and Ferreira,R.S. Profiling selectivity of chagasin mutants towards cysteine proteases cruzain or cathepsin L through molecular dynamics simulations J Biomol Struct Dyn (2020) , 1-13. PubMed Europe PubMed DOI

2019

Barbosa da Silva,E., Dall,E., Briza,P., Brandstetter,H. and Ferreira,R.S. Cruzain structures: apocruzain and cruzain bound to S-methyl thiomethanesulfonate and implications for drug design Acta Crystallogr F Struct Biol Commun (2019) 75, 419-427. PubMed Europe PubMed DOI S
Bhowmick,S., Chorge,R.D., Jangam,C.S., Bharatrao,L.D., Patil,P.C., Chikhale,R.V. and Islam,M.A. Identification of potential cruzain inhibitors using de novo design, molecular docking and dynamics simulations studies J Biomol Struct Dyn (2019) , 1-11. PubMed Europe PubMed DOI I
Boudreau,P.D., Miller,B.W., McCall,L.I., Almaliti,J., Reher,R., Hirata,K., Le,T., Siqueira-Neto,J.L., Hook,V. and Gerwick,W.H. Design of gallinamide A analogs as potent inhibitors of the cysteine proteases human cathepsin L and Trypanosoma cruzi cruzain J Med Chem (2019) 62, 9026-9044. PubMed Europe PubMed DOI I
Cianni,L., Feldmann,C.W., Gilberg,E., Gutschow,M., Juliano,L., Leitao,A., Bajorath,J. and Montanari,C.A. Can cysteine protease cross-class inhibitors achieve selectivity? J Med Chem (2019) 62, 10497-10525. PubMed Europe PubMed DOI I
Fabian,L., Martini,M.F., Sarduy,E.S., Estrin,D.A. and Moglioni,A.G. Evaluation of quinoxaline compounds as ligands of a site adjacent to S2 (AS2) of cruzain Bioorg Med Chem Lett (2019) 29, 2197-2202. PubMed Europe PubMed DOI I
Ferreira,R.A.A., Pauli,I., Sampaio,T.S., de Souza,M.L., Ferreira,L.L.G., Magalhaes,L.G., Rezende CO Jr, Ferreira,R.S., Krogh,R., Dias,L.C. and Andricopulo,A.D. Structure-based and molecular modeling studies for the discovery of cyclic imides as reversible cruzain inhibitors with potent anti-Trypanosoma cruzi activity Front Chem (2019) 7, 798-798. PubMed Europe PubMed DOI PMC EPMC I
Gomes,J.C., Cianni,L., Ribeiro,J., Dos Reis Rocho,F., da Costa Martins Silva,S., Batista,P.H.J., Moraes,C.B., Franco,C.H., Freitas-Junior,L.H.G., Kenny,P.W., Leitao,A., Burtoloso,A.C.B., De Vita,D. and Montanari,C.A. Synthesis and structure-activity relationship of nitrile-based cruzain inhibitors incorporating a trifluoroethylamine-based P2 amide replacement Bioorg Med Chem (2019) , 115083-115083. PubMed Europe PubMed DOI I
Hernandez Alvarez,L., Barreto Gomes,D.E., Hernandez Gonzalez,J.E. and Pascutti,P.G. Dissecting a novel allosteric mechanism of cruzain: a computer-aided approach PLoS ONE (2019) 14, e0211227-e0211227. PubMed Europe PubMed DOI PMC EPMC
Herrera-Mayorga,V., Lara-Ramirez,E.E., Chacon-Vargas,K.F., Aguirre-Alvarado,C., Rodriguez-Paez,L., Alcantara-Farfan,V., Cordero-Martinez,J., Nogueda-Torres,B., Reyes-Espinosa,F., Bocanegra-Garcia,V. and Rivera,G. Structure-based virtual screening and in vitro evaluation of new Trypanosoma cruzi cruzain inhibitors Int J Mol Sci (2019) 20 PubMed Europe PubMed DOI I
Luchi,A.M., Villafane,R.N., Gomez Chavez,J.L., Bogado,M.L., Angelina,E.L. and Peruchena,N.M. Combining charge density analysis with machine learning tools to investigate the cruzain inhibition mechanism ACS Omega (2019) 4, 19582-19594. PubMed Europe PubMed DOI PMC EPMC I
Monteiro,M.E., Lechuga,G., Lara,L.S., Souto,B.A., Vigano,M.G., Bourguignon,S.C., Calvet,C.M., Oliveira,F.O.R., Jr., Alves,C.R., Souza-Silva,F., Santos,M.S. and Pereira,M.C.S. Synthesis, structure-activity relationship and trypanocidal activity of pyrazole-imidazoline and new pyrazole-tetrahydropyrimidine hybrids as promising chemotherapeutic agents for Chagas disease Eur J Med Chem (2019) 182, 111610-111610. PubMed Europe PubMed DOI I
Reyes-Espinosa,F., Juarez-Saldivar,A., Palos,I., Herrera-Mayorga,V., Garcia-Perez,C. and Rivera,G. In silico analysis of homologous heterodimers of cruzipain-chagasin from structural models built by homology Int J Mol Sci (2019) 20 PubMed Europe PubMed DOI
Santos,L.H., Waldner,B.J., Fuchs,J.E., Pereira,G.A.N., Liedl,K.R., Caffarena,E.R. and Ferreira,R.S. Understanding structure-activity relationships for trypanosomal cysteine protease inhibitors by simulations and free energy calculations J Chem Inf Model (2019) 59, 137-148. PubMed Europe PubMed DOI I
Sartori,G.R., Leitao,A., Montanari,C.A. and Laughton,C.A. Ligand-induced conformational selection predicts the selectivity of cysteine protease inhibitors PLoS ONE (2019) 14, e0222055-e0222055. PubMed Europe PubMed DOI I
Vieira,R.P., Santos,V.C. and Ferreira,R.S. Structure-based approaches targeting parasite cysteine proteases Curr Med Chem (2019) 26, 4435-4453. PubMed Europe PubMed DOI I

2018

Cianni,L., Sartori,G., Rosini,F., De Vita,D., Pires,G., Lopes,B.R., Leitao,A., Burtoloso,A.C.B. and Montanari,C.A. Leveraging the cruzain S3 subsite to increase affinity for reversible covalent inhibitors Bioorg Chem (2018) 79, 285-292. PubMed Europe PubMed DOI I
da Silva-Junior,E.F., Barcellos Franca,P.H., Ribeiro,F.F., Bezerra Mendonca-Junior,F.J., Scotti,L., Scotti,M.T., de Aquino,T.M. and de Araujo-Junior,J.X. Molecular docking studies applied to a dataset of cruzain inhibitors Curr Comput Aided Drug Des (2018) 14, 68-78. PubMed Europe PubMed DOI I
Dos Santos,A.M., Cianni,L., De Vita,D., Rosini,F., Leitao,A., Laughton,C.A., Lameira,J. and Montanari,C.A. Experimental study and computational modelling of cruzain cysteine protease inhibition by dipeptidyl nitriles Phys Chem Chem Phys (2018) 20, 24317-24328. PubMed Europe PubMed DOI I
Giroud,M., Dietzel,U., Anselm,L., Banner,D., Kuglstatter,A., Benz,J., Blanc,J.B., Gaufreteau,D., Liu,H., Lin,X., Stich,A., Kuhn,B., Schuler,F., Kaiser,M., Brun,R., Schirmeister,T., Kisker,C., Diederich,F. and Haap,W. Repurposing a library of human cathepsin L ligands: identification of macrocyclic lactams as potent rhodesain and Trypanosoma brucei inhibitors J Med Chem (2018) 61, 3350-3369. PubMed Europe PubMed DOI S I
Martins,L.C., Torres,P.H.M., de Oliveira,R.B., Pascutti,P.G., Cino,E.A. and Ferreira,R.S. Investigation of the binding mode of a novel cruzain inhibitor by docking, molecular dynamics, ab initio and MM/PBSA calculations J Comput Aided Mol Des (2018) 32, 591-605. PubMed Europe PubMed DOI I
Rocha,D.A., Silva,E.B., Fortes,I.S., Lopes,M.S., Ferreira,R.S. and Andrade,S.F. Synthesis and structure-activity relationship studies of cruzain and rhodesain inhibitors Eur J Med Chem (2018) 157, 1426-1459. PubMed Europe PubMed DOI I
Royo,S., Schirmeister,T., Kaiser,M., Jung,S., Rodriguez,S., Bautista,J.M. and Gonzalez,F.V. Antiprotozoal and cysteine proteases inhibitory activity of dipeptidyl enoates Bioorg Med Chem (2018) 26, 4624-4634. PubMed Europe PubMed DOI I
Salas-Sarduy,E., Landaburu,L.U., Karpiak,J., Madauss,K.P., Cazzulo,J.J., Aguero,F. and Alvarez,V.E. Author Correction: Novel scaffolds for inhibition of cruzipain identified from high-throughput screening of anti-kinetoplastid chemical boxes Sci Rep (2018) 8, 8743-8743. PubMed Europe PubMed DOI PMC EPMC I
Saraiva,A.P.B., Miranda,R.M., Valente,R.P.P., Araujo,J.O., Souza,R.N.B., Costa,C.H.S., Oliveira,A.R.S., Almeida,M.O., Figueiredo,A.F., Ferreira,J.E.V., Alves,C.N. and Honorio,K.M. Molecular description of alpha-keto-based inhibitors of cruzain with activity against Chagas disease combining 3D-QSAR studies and molecular dynamics Chem Biol Drug Des (2018) 92, 1475-1487. PubMed Europe PubMed DOI I
Serrano,M., Gonzalez,V., Ray,S., Chavez,M.D. and Narayan,M. Identification of structure-stabilizing interactions in enzymes: a novel mechanism to impact enzyme activity Cell Biochem Biophys (2018) 76, 59-71. PubMed Europe PubMed DOI
Zhai,X. and Meek,T.D. Catalytic mechanism of cruzain from Trypanosoma cruzi as determined from solvent kinetic isotope effects of steady-state and pre-steady-state kinetics Biochemistry (2018) 57, 3176-3190. PubMed Europe PubMed DOI

2017

Braga,S.F., Martins,L.C., da Silva,E.B., Sales Junior,P.A., Murta,S.M., Romanha,A.J., Soh,W.T., Brandstetter,H., Ferreira,R.S. and de Oliveira,R.B. Synthesis and biological evaluation of potential inhibitors of the cysteine proteases cruzain and rhodesain designed by molecular simplification Bioorg Med Chem (2017) 25, 1889-1900. PubMed Europe PubMed DOI I
Burtoloso,A.C., de Albuquerque,S., Furber,M., Gomes,J.C., Goncalez,C., Kenny,P.W., Leitao,A., Montanari,C.A., Quilles JC Junior, Ribeiro,J.F. and Rocha,J.R. Anti-trypanosomal activity of non-peptidic nitrile-based cysteine protease inhibitors PLoS Negl Trop Dis (2017) 11, e0005343-e0005343. PubMed Europe PubMed DOI I
de Souza,A.S., de Oliveira,M.T. and Andricopulo,A.D. Development of a pharmacophore for cruzain using oxadiazoles as virtual molecular probes: quantitative structure-activity relationship studies J Comput Aided Mol Des (2017) 31, 801-816. PubMed Europe PubMed DOI I
Ferreira,L.G. and Andricopulo,A.D. Targeting cysteine proteases in trypanosomatid disease drug discovery Pharmacol Ther (2017) 180, 49-61. PubMed Europe PubMed DOI T
Palos,I., Lara-Ramirez,E.E., Lopez-Cedillo,J.C., Garcia-Perez,C., Kashif,M., Bocanegra-Garcia,V., Nogueda-Torres,B. and Rivera,G. Repositioning FDA drugs as potential cruzain inhibitors from Trypanosoma cruzi: virtual screening, In vitro and in vivo studies Molecules (2017) 22 PubMed Europe PubMed DOI I
Pauli,I., Ferreira,L.G., de Souza,M.L., Oliva,G., Ferreira,R.S., Dessoy,M.A., Slafer,B.W., Dias,L.C. and Andricopulo,A.D. Molecular modeling and structure-activity relationships for a series of benzimidazole derivatives as cruzain inhibitors Future Med Chem (2017) 9, 641-657. PubMed Europe PubMed DOI I
Salas-Sarduy,E., Landaburu,L.U., Karpiak,J.X., Madauss,K.P., Cazzulo,J.J., Aguero,F. and Alvarez,V.E. Novel scaffolds for inhibition of cruzipain identified from high-throughput screening of anti-kinetoplastid chemical boxes Sci Rep (2017) 7, 12073-12073. PubMed Europe PubMed DOI I
Silva,D.G., Ribeiro,J.F.R., De Vita,D., Cianni,L., Franco,C.H., Freitas-Junior,L.H., Moraes,C.B., Rocha,J.R., Burtoloso,A.C.B., Kenny,P.W., Leitao,A. and Montanari,C.A. A comparative study of warheads for design of cysteine protease inhibitors Bioorg Med Chem Lett (2017) 27, 5031-5035. PubMed Europe PubMed DOI I
Silva,D.G., Rocha,J.R., Sartori,G.R. and Montanari,C.A. Highly predictive hologram QSAR models of nitrile-containing cruzain inhibitors J Biomol Struct Dyn (2017) 35, 3232-3249. PubMed Europe PubMed DOI I

2016

Cerny,N., Sanchez Alberti,A., Bivona,A.E., De Marzi,M.C., Frank,F.M., Cazorla,S.I. and Malchiodi,E.L. Coadministration of cruzipain and GM-CSF DNAs, a new immunotherapeutic vaccine against Trypanosoma cruzi infection Hum Vaccin Immunother (2016) 12, 438-450. PubMed Europe PubMed DOI U
de Moraes Gomes,P.A., de Oliveira Barbosa,M., Farias Santiago,E., de Oliveira Cardoso,M.V., Capistrano Costa,N.T., Hernandes,M.Z., Moreira,D.R., Da Silva,A.C., Dos Santos,T.A., Pereira,V.R., Brayner Dos Santosd,F.A., do Nascimento Pereira,G.A., Ferreira,R.S. and Leite,A.C. New 1,3-thiazole derivatives and their biological and ultrastructural effects on Trypanosoma cruzi Eur J Med Chem (2016) 121, 387-398. PubMed Europe PubMed DOI I
Ferrero,M.R., Heins,A.M., Soprano,L.L., Acosta,D.M., Esteva,M.I., Jacobs,T. and Duschak,V.G. Involvement of sulfates from cruzipain, a major antigen of Trypanosoma cruzi, in the interaction with immunomodulatory molecule Siglec-E Med Microbiol Immunol (2016) 205, 21-35. PubMed Europe PubMed DOI
Hoelz,L.V., Leal,V.F., Rodrigues,C.R., Pascutti,P.G., Albuquerque,M.G., Muri,E.M. and Dias,L.R. Molecular dynamics simulations of the free and inhibitor-bound cruzain systems in aqueous solvent: insights on the inhibition mechanism in acidic pH J Biomol Struct Dyn (2016) 34, 1969-1978. PubMed Europe PubMed DOI
Jefferson,T., McShan,D., Warfield,J. and Ogungbe,I.V. Screening and identification of inhibitors of Trypanosoma brucei cathepsin L with antitrypanosomal activity Chem Biol Drug Des (2016) 87, 154-158. PubMed Europe PubMed DOI I
Latorre,A., Schirmeister,T., Kesselring,J., Jung,S., Johe,P., Hellmich,U.A., Heilos,A., Engels,B., Krauth-Siegel,R.L., Dirdjaja,N., Bou-Iserte,L., Rodriguez,S. and Gonzalez,F.V. Dipeptidyl nitroalkenes as potent reversible inhibitors of cysteine proteases rhodesain and cruzain ACS Med Chem Lett (2016) 7, 1073-1076. PubMed Europe PubMed DOI PMC EPMC I
Sbaraglini,M.L., Bellera,C.L., Fraccaroli,L., Larocca,L., Carrillo,C., Talevi,A. and Alba Soto,C.D. Novel cruzipain inhibitors for the chemotherapy of chronic Chagas disease Int J Antimicrob Agents (2016) 48, 91-95. PubMed Europe PubMed DOI I
Silva-Junior,E.F., Silva,E.P., Franca,P.H., Silva,J.P., Barreto,E.O., Silva,E.B., Ferreira,R.S., Gatto,C.C., Moreira,D.R., Siqueira-Neto,J.L., Mendonca-Junior,F.J., Lima,M.C., Bortoluzzi,J.H., Scotti,M.T., Scotti,L., Meneghetti,M.R., Aquino,T.M. and Araujo-Junior,J.X. Design, synthesis, molecular docking and biological evaluation of thiophen-2-iminothiazolidine derivatives for use against Trypanosoma cruzi Bioorg Med Chem (2016) 24, 4228-4240. PubMed Europe PubMed DOI I

2015

Arafet,K., Ferrer,S. and Moliner,V. First quantum mechanics/molecular mechanics studies of the inhibition mechanism of cruzain by peptidyl halomethyl ketones Biochemistry (2015) 54, 3381-3391. PubMed Europe PubMed DOI I
Avelar,L.A., Camilo,C.D., de Albuquerque,S., Fernandes,W.B., Goncalez,C., Kenny,P.W., Leitao,A., McKerrow,J.H., Montanari,C.A., Orozco,E.V., Ribeiro,J.F., Rocha,J.R., Rosini,F. and Saidel,M.E. Molecular design, synthesis and trypanocidal activity of dipeptidyl nitriles as cruzain inhibitors PLoS Negl Trop Dis (2015) 9, e0003916-e0003916. PubMed Europe PubMed DOI PMC EPMC I
Branquinha,M.H., Oliveira,S.S., Sangenito,L.S., Sodre,C.L., Kneipp,L.F., D'Avila-Levy,C.M. and Santos,A.L. Cruzipain: an update on its potential as chemotherapy target against the human pathogen Trypanosoma cruzi Curr Med Chem (2015) 22, 2225-2235. PubMed Europe PubMed V
de Almeida,L., Alves,K.F., Maciel-Rezende,C.M., Jesus Lde,O., Pires,F.R., Junior,C.V., Izidoro,M.A., Judice,W.A., Dos Santos,M.H. and Marques,M.J. Benzophenone derivatives as cysteine protease inhibitors and biological activity against Leishmania(L.) amazonensis amastigotes Biomed Pharmacother (2015) 75, 93-99. PubMed Europe PubMed DOI I
Espindola,J.W., Cardoso,M.V., Filho,G.B., Oliveira E Silva,D.A., Moreira,D.R., Bastos,T.M., Simone,C.A., Soares,M.B., Villela,F.S., Ferreira,R.S., Castro,M.C., Pereira,V.R., Murta,S.M., Sales Junior,P.A., Romanha,A.J. and Leite,A.C. Synthesis and structure-activity relationship study of a new series of antiparasitic aryloxyl thiosemicarbazones inhibiting Trypanosoma cruzi cruzain Eur J Med Chem (2015) 101, 818-835. PubMed Europe PubMed DOI I
Ferrao,P.M., D'Avila-Levy,C.M., Araujo-Jorge,T.C., Degrave,W.M., Goncalves,A.S., Garzoni,L.R., Lima,A.P., Feige,J.J., Bailly,S., Mendonca-Lima,L. and Waghabi,M.C. Cruzipain activates latent TGF-beta from host cells during T. cruzi invasion PLoS ONE (2015) 10, e0124832- PubMed Europe PubMed DOI PMC EPMC I
Fonseca,N.C., da Cruz,L.F., da Silva Villela,F., do Nascimento Pereira,G.A., de Siqueira-Neto,J.L., Kellar,D., Suzuki,B.M., Ray,D., de Souza,T.B., Alves,R.J., Junior,P.A., Romanha,A.J., Murta,S.M., McKerrow,J.H., Caffrey,C.R., de Oliveira,R.B. and Ferreira,R.S. Synthesis of a sugar-based thiosemicarbazone series and structure-activity relationship versus the parasite cysteine proteases rhodesain, cruzain, and Schistosoma mansoni cathepsin B1 Antimicrob Agents Chemother (2015) 59, 2666-2677. PubMed Europe PubMed DOI I
Martinez-Mayorga,K., Byler,K.G., Ramirez-Hernandez,A.I. and Terrazas-Alvares,D.E. Cruzain inhibitors: efforts made, current leads and a structural outlook of new hits Drug Discov Today (2015) 20, 890-898. PubMed Europe PubMed DOI I
Neitz,R.J., Bryant,C., Chen,S., Gut,J., Hugo Caselli,E., Ponce,S., Chowdhury,S., Xu,H., Arkin,M.R., Ellman,J.A. and Renslo,A.R. Tetrafluorophenoxymethyl ketone cruzain inhibitors with improved pharmacokinetic properties as therapeutic leads for Chagas' disease Bioorg Med Chem Lett (2015) 25, 4834-4837. PubMed Europe PubMed DOI I

2014

Batista,C.M., Medeiros,L.C., Eger,I. and Soares,M.J. mAb CZP-315.D9: an antirecombinant cruzipain monoclonal antibody that specifically labels the reservosomes of Trypanosoma cruzi epimastigotes Biomed Res Int (2014) 2014, 714749-714749. PubMed Europe PubMed DOI PMC EPMC
Ferreira,R.S., Dessoy,M.A., Pauli,I., Souza,M.L., Krogh,R., Sales,A.I., Oliva,G., Dias,L.C. and Andricopulo,A.D. Synthesis, biological evaluation, and structure-activity relationships of potent noncovalent and nonpeptidic cruzain inhibitors as anti-Trypanosoma cruzi agents J Med Chem (2014) 57, 2380-2392. PubMed Europe PubMed DOI I
Maeda,F.Y., Cortez,C., Izidoro,M.A., Juliano,L. and Yoshida,N. Fibronectin-degrading activity of Trypanosoma cruzi cysteine proteinase plays a role in host cell invasion Infect Immun (2014) 82, 5166-5174. PubMed Europe PubMed DOI
Miao,Q., Santamaria,C., Bailey,D., Genest,J., Ward,B.J. and Ndao,M. Apolipoprotein A-I truncations in Chagas disease are caused by cruzipain, the major cysteine protease of Trypanosoma cruzi Am J Pathol (2014) 184, 976-984. PubMed Europe PubMed DOI
Ndao,M., Beaulieu,C., Black,W.C., Isabel,E., Vasquez-Camargo,F., Nath-Chowdhury,M., Masse,F., Mellon,C., Methot,N. and Nicoll-Griffith,D.A. Reversible cysteine protease inhibitors show promise for a Chagas disease cure Antimicrob Agents Chemother (2014) 58, 1167-1178. PubMed Europe PubMed DOI I
Rodrigues,A.C., Ortiz,P.A., Costa-Martins,A.G., Neves,L., Garcia,H.A., Alves,J.M., Camargo,E.P., Alfieri,S.C., Gibson,W. and Teixeira,M.M. Congopain genes diverged to become specific to Savannah, Forest and Kilifi subgroups of Trypanosoma congolense, and are valuable for diagnosis, genotyping and phylogenetic inferences Infect Genet Evol (2014) 23, 20-31. PubMed Europe PubMed DOI

2013

Assis,D.M., Gontijo,V.S., de Oliveira Pereira,I., Santos,J.A., Camps,I., Nagem,T.J., Ellena,J., Izidoro,M.A., Dos Santos Tersariol,I.L., de Barros,N.M., Doriguetto,A.C., Dos Santos,M.H. and Juliano,M.A. Inhibition of cysteine proteases by a natural biflavone: behavioral evaluation of fukugetin as papain and cruzain inhibitor J Enzyme Inhib Med Chem (2013) 28, 661-670. PubMed Europe PubMed DOI S I
Bellera,C.L., Balcazar,D.E., Alberca,L., Labriola,C.A., Talevi,A. and Carrillo,C. Application of computer-aided drug repurposing in the search of new cruzipain inhibitors: discovery of amiodarone and bromocriptine inhibitory effects J Chem Inf Model (2013) 53, 2402-2408. PubMed Europe PubMed DOI I
Ponce,N.E., Carrera-Silva,E.A., Pellegrini,A.V., Cazorla,S.I., Malchiodi,E.L., Lima,A.P., Gea,S. and Aoki,M.P. Trypanosoma cruzi, the causative agent of Chagas disease, modulates interleukin-6-induced STAT3 phosphorylation via gp130 cleavage in different host cells Biochim Biophys Acta (2013) 1832, 485-494. PubMed Europe PubMed DOI
Wiggers,H.J., Rocha,J.R., Fernandes,W.B., Sesti-Costa,R., Carneiro,Z.A., Cheleski,J., Da Silva,A.B., Juliano,L., Cezari,M.H., Silva,J.S., McKerrow,J.H. and Montanari,C.A. Non-peptidic cruzain inhibitors with trypanocidal activity discovered by virtual screening and in vitro assay PLoS Negl Trop Dis (2013) 7, e2370-e2370. PubMed Europe PubMed DOI PMC EPMC I

2012

Alvarez,V.E., Niemirowicz,G.T. and Cazzulo,J.J. The peptidases of Trypanosoma cruzi: digestive enzymes, virulence factors, and mediators of autophagy and programmed cell death Biochim Biophys Acta (2012) 1824, 195-206. PubMed Europe PubMed DOI V
Cazzulo,J.J. Cruzipain [ISSN:978-0-12-407743-0] (2012) 3, 1913-1918. DOI
Lavrado,J., Mackey,Z., Hansell,E., McKerrow,J.H., Paulo,A. and Moreira,R. Antitrypanosomal and cysteine protease inhibitory activities of alkyldiamine cryptolepine derivatives Bioorg Med Chem Lett (2012) 22, 6256-6260. PubMed Europe PubMed DOI I
Lee,G.M., Balouch,E., Goetz,D.H., Lazic,A., McKerrow,J.H. and Craik,C.S. Mapping inhibitor binding modes on an active cysteine protease via nuclear magnetic resonance spectroscopy Biochemistry (2012) 51, 10087-10098. PubMed Europe PubMed DOI E S I
Lima,L., Ortiz,P.A., da Silva,F.M., Alves,J.M., Serrano,M.G., Cortez,A.P., Alfieri,S.C., Buck,G.A. and Teixeira,M.M. Repertoire, genealogy and genomic organization of cruzipain and homologous genes in Trypanosoma cruzi, T. cruzi-like and other trypanosome species PLoS ONE (2012) 7, e38385-e38385. PubMed Europe PubMed DOI PMC EPMC
Lozano,N.B., Maltarollo,V.G., Weber,K.C., Honorio,K.M., Guido,R.V., Andricopulo,A.D. and Da Silva,A.B. Molecular features for antitrypanosomal activity of thiosemicarbazones revealed by OPS-PLS QSAR studies Med Chem (2012) 8, 1045-1056. PubMed Europe PubMed I
Mendez-Lucio,O., Romo-Mancillas,A., Medina-Franco,J.L. and Castillo,R. Computational study on the inhibition mechanism of cruzain by nitrile-containing molecules J Mol Graph Model (2012) 35, 28-35. PubMed Europe PubMed DOI I
Rogers,K.E., Keranen,H., Durrant,J.D., Ratnam,J., Doak,A., Arkin,M.R. and McCammon,J.A. Novel cruzain inhibitors for the treatment of Chagas' disease Chem Biol Drug Des (2012) 80, 398-405. PubMed Europe PubMed DOI I
Uehara,L.A., Moreira,O.C., Oliveira,A.C., Azambuja,P., Lima,A.P., Britto,C., Dos Santos,A.L., Branquinha,M.H. and D'Avila-Levy,C.M. Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut PLoS Negl Trop Dis (2012) 6, e1958-e1958. PubMed Europe PubMed DOI PMC EPMC
Yang,P.Y., Wang,M., Li,L., Wu,H., He,C.Y. and Yao,S.Q. Design, synthesis and biological evaluation of potent azadipeptide nitrile inhibitors and activity-based probes as promising anti-Trypanosoma brucei agents Chemistry (2012) 18, 6528-6541. PubMed Europe PubMed DOI I

2011

Acosta,D.M., Soprano,L.L., Ferrero,M., Landoni,M., Esteva,M.I., Couto,A.S. and Duschak,V.G. A striking common O-linked N-acetylglucosaminyl moiety between cruzipain and myosin Parasite Immunol (2011) 33, 363-370. PubMed Europe PubMed DOI
Boulange,A.F., Khamadi,S.A., Pillay,D., Coetzer,T.H. and Authie,E. Production of congopain, the major cysteine protease of Trypanosoma (Nannomonas) congolense, in Pichia pastoris reveals unexpected dimerisation at physiological pH Protein Expr Purif (2011) 75, 95-103. PubMed Europe PubMed DOI E
Caffrey,C.R., Lima,A.P. and Steverding,D. Cysteine peptidases of kinetoplastid parasites Adv Exp Med Biol (2011) 712, 84-99. PubMed Europe PubMed DOI V
Caputto,M.E., Fabian,L.E., Benitez,D., Merlino,A., Rios,N., Cerecetto,H., Moltrasio,G.Y., Moglioni,A.G., Gonzalez,M. and Finkielsztein,L.M. Thiosemicarbazones derived from 1-indanones as new anti-Trypanosoma cruzi agents Bioorg Med Chem (2011) 19, 6818-6826. PubMed Europe PubMed DOI I
Doyle,P.S., Zhou,Y.M., Hsieh,I., Greenbaum,D.C., McKerrow,J.H. and Engel,J.C. The Trypanosoma cruzi protease cruzain mediates immune evasion PLoS Pathog (2011) 7, e1002139-e1002139. PubMed Europe PubMed DOI PMC EPMC
Sajid,M., Robertson,S.A., Brinen,L.S. and McKerrow,J.H. Cruzain : the path from target validation to the clinic Adv Exp Med Biol (2011) 712, 100-115. PubMed Europe PubMed DOI V

2010

Beaulieu,C., Isabel,E., Fortier,A., Masse,F., Mellon,C., Methot,N., Ndao,M., Nicoll-Griffith,D., Lee,D., Park,H. and Black,W.C. Identification of potent and reversible cruzipain inhibitors for the treatment of Chagas disease Bioorg Med Chem Lett (2010) 20, 7444-7449. PubMed Europe PubMed DOI I
Benitez-Hernandez,I., Mendez-Enriquez,E., Ostoa,P., Fortoul,T., Ramirez,J.A., Stempin,C., Cerban,F., Soldevila,G. and Garcia-Zepeda,E.A. Proteolytic cleavage of chemokines by Trypanosoma cruzi's cruzipain inhibits chemokine functions by promoting the generation of antagonists Immunobiology (2010) 215, 413-426. PubMed Europe PubMed DOI
Brak,K., Kerr,I.D., Barrett,K.T., Fuchi,N., Debnath,M., Ang,K., Engel,J.C., McKerrow,J.H., Doyle,P.S., Brinen,L.S. and Ellman,J.A. Nonpeptidic tetrafluorophenoxymethyl ketone cruzain inhibitors as promising new leads for Chagas disease chemotherapy J Med Chem (2010) 53, 1763-1773. PubMed Europe PubMed DOI I
Cazorla,S.I., Frank,F.M., Becker,P.D., Arnaiz,M., Mirkin,G.A., Corral,R.S., Guzman,C.A. and Malchiodi,E.L. Redirection of the immune response to the functional catalytic domain of the cystein proteinase cruzipain improves protective immunity against Trypanosoma cruzi infection J Infect Dis (2010) 202, 136-144. PubMed Europe PubMed DOI
Durrant,J.D., Keranen,H., Wilson,B.A. and McCammon,J.A. Computational identification of uncharacterized cruzain binding sites PLoS Negl Trop Dis (2010) 4, e676-e676. PubMed Europe PubMed DOI PMC EPMC
Ferreira,R.S., Simeonov,A., Jadhav,A., Eidam,O., Mott,B.T., Keiser,M.J., McKerrow,J.H., Maloney,D.J., Irwin,J.J. and Shoichet,B.K. Complementarity between a docking and a high-throughput screen in discovering new cruzain inhibitors J Med Chem (2010) 53, 4891-4905. PubMed Europe PubMed DOI I
Luci,D., Lea,W., Ferreira,R., Shoichet,B., Simeonov,A., Rodriguez,A., Jadhav,A. and Maloney,D.J. Reversible and non-covalent benzimidazole-based in vivo lead for Chagas disease [ISSN:NBK133417] (2010) PubMed Europe PubMed I
Mott,B.T., Ferreira,R.S., Simeonov,A., Jadhav,A., Ang,K.K., Leister,W., Shen,M., Silveira,J.T., Doyle,P.S., Arkin,M.R., McKerrow,J.H., Inglese,J., Austin,C.P., Thomas,C.J., Shoichet,B.K. and Maloney,D.J. Identification and optimization of inhibitors of trypanosomal cysteine proteases: cruzain, rhodesain, and TbCatB J Med Chem (2010) 53, 52-60. PubMed Europe PubMed DOI S I
Pillay,D., Boulange,A.F. and Coetzer,T.H. Expression, purification and characterisation of two variant cysteine peptidases from Trypanosoma congolense with active site substitutions Protein Expr Purif (2010) 74, 264-271. PubMed Europe PubMed DOI
Rodrigues,G.C., Aguiar,A.P., da Silva Goncalves Vianez Jr., Macrae,A., De Melo,A.C.N. and Vermelho,A.B. Peptidase inhibitors as a possible therapeutic strategy for Chagas disease Curr Enzyme Inhib (2010) 6, 183-194. DOI I

2009

Bryant,C., Kerr,I.D., Debnath,M., Ang,K.K., Ratnam,J., Ferreira,R.S., Jaishankar,P., Zhao,D., Arkin,M.R., McKerrow,J.H., Brinen,L.S. and Renslo,A.R. Novel non-peptidic vinylsulfones targeting the S2 and S3 subsites of parasite cysteine proteases Bioorg Med Chem Lett (2009) 19, 6218-6221. PubMed Europe PubMed DOI S I
Caffrey,C.R. and Steverding,D. Kinetoplastid papain-like cysteine peptidases Mol Biochem Parasitol (2009) 167, 12-19. PubMed Europe PubMed DOI I
dos Santos Filho,J.M., Leite,A.C., de Oliveira,B.G., Moreira,D.R., Lima,M.S., Soares,M.B. and Leite,L.F. Design, synthesis and cruzain docking of 3-(4-substituted-aryl)-1,2,4-oxadiazole-N-acylhydrazones as anti-Trypanosoma cruzi agents Bioorg Med Chem (2009) 17, 6682-6691. PubMed Europe PubMed DOI I
Duschak,V.G. and Couto,A.S. Cruzipain, the major cysteine protease of Trypanosoma cruzi: a sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review Curr Med Chem (2009) 16, 3174-3202. PubMed Europe PubMed DOI V
Ferreira,R.S., Bryant,C., Ang,K.K., McKerrow,J.H., Shoichet,B.K. and Renslo,A.R. Divergent modes of enzyme inhibition in a homologous structure-activity series J Med Chem (2009) 52, 5005-5008. PubMed Europe PubMed DOI I
Huson,L.E., Authie,E., Boulange,A.F., Goldring,J.P. and Coetzer,T.H. Modulation of the immunogenicity of the Trypanosoma congolense cysteine protease, congopain, through complexation with alpha(2)-macroglobulin Vet Res (2009) 40, 52-52. PubMed Europe PubMed DOI PMC EPMC
Kerr,I.D., Lee,J.H., Farady,C.J., Marion,R., Rickert,M., Sajid,M., Pandey,K.C., Caffrey,C.R., Legac,J., Hansell,E., McKerrow,J.H., Craik,C.S., Rosenthal,P.J. and Brinen,L.S. Vinyl sulfones as antiparasitic agents and a structural basis for drug design J Biol Chem (2009) 284, 25697-25703. PubMed Europe PubMed DOI PMC EPMC S I
Romeiro,N.C., Aguirre,G., Hernandez,P., Gonzalez,M., Cerecetto,H., Aldana,I., Perez-Silanes,S., Monge,A., Barreiro,E.J. and Lima,L.M. Synthesis, trypanocidal activity and docking studies of novel quinoxaline-N-acylhydrazones, designed as cruzain inhibitors candidates Bioorg Med Chem (2009) 17, 641-652. PubMed Europe PubMed DOI I

2008

Acosta,D.M., Arnaiz,M.R., Esteva,M.I., Barboza,M., Stivale,D., Orlando,U.D., Torres,S., Laucella,S.A., Couto,A.S. and Duschak,V.G. Sulfates are main targets of immune responses to cruzipain and are involved in heart damage in BALB/c immunized mice Int Immunol (2008) 20, 461-470. PubMed Europe PubMed DOI
Brak,K., Doyle,P.S., McKerrow,J.H. and Ellman,J.A. Identification of a new class of nonpeptidic inhibitors of cruzain J Am Chem Soc (2008) 130, 6404-6410. PubMed Europe PubMed DOI PMC EPMC I
Cazorla,S.I., Frank,F.M., Becker,P.D., Corral,R.S., Guzman,C.A. and Malchiodi,E.L. Prime-boost immunization with cruzipain co-administered with MALP-2 triggers a protective immune response able to decrease parasite burden and tissue injury in an experimental Trypanosoma cruzi infection model Vaccine (2008) 26, 1999-2009. PubMed Europe PubMed DOI
Chen,Y.T., Lira,R., Hansell,E., McKerrow,J.H. and Roush,W.R. Synthesis of macrocyclic trypanosomal cysteine protease inhibitors Bioorg Med Chem Lett (2008) 18, 5860-5863. PubMed Europe PubMed DOI PMC EPMC I
Frank,F.M., Cazorla,S.I., Sartori,M.J. and Corral,R.S. Elicitation of specific, Th1-biased immune response precludes skeletal muscle damage in cruzipain-vaccinated mice Exp Mol Pathol (2008) 84, 64-70. PubMed Europe PubMed DOI
Freitas,R.F., Oprea,T.I. and Montanari,C.A. 2D QSAR and similarity studies on cruzain inhibitors aimed at improving selectivity over cathepsin L Bioorg Med Chem (2008) 16, 838-853. PubMed Europe PubMed DOI I
Guido,R.V., Trossini,G.H., Castilho,M.S., Oliva,G., Ferreira,E.I. and Andricopulo,A.D. Structure-activity relationships for a class of selective inhibitors of the major cysteine protease from Trypanosoma cruzi J Enzyme Inhib Med Chem (2008) 23, 964-973. PubMed Europe PubMed DOI I
Jaishankar,P., Hansell,E., Zhao,D.M., Doyle,P.S., McKerrow,J.H. and Renslo,A.R. Potency and selectivity of P2/P3-modified inhibitors of cysteine proteases from trypanosomes Bioorg Med Chem Lett (2008) 18, 624-628. PubMed Europe PubMed DOI I
Scharfstein,J. and Lima,A.P. Roles of naturally occurring protease inhibitors in the modulation of host cell signaling and cellular invasion by Trypanosoma cruzi Subcell Biochem (2008) 47, 140-154. PubMed Europe PubMed DOI
Zanatta,N., Amaral,S.S., Dos Santos,J.M., de Mello,D.L., Fernandes,L., Bonacorso,H.G., Martins,M.A., Andricopulo,A.D. and Borchhardt,D.M. Convergent synthesis and cruzain inhibitory activity of novel 2-(N'-benzylidenehydrazino)-4-trifluoromethyl-pyrimidines Bioorg Med Chem (2008) 16, 10236-10243. PubMed Europe PubMed DOI I

2007

Doyle,P.S., Zhou,Y.M., Engel,J.C. and McKerrow,J.H. A cysteine protease inhibitor cures Chagas' disease in an immunodeficient-mouse model of infection Antimicrob Agents Chemother (2007) 51, 3932-3939. PubMed Europe PubMed DOI PMC EPMC I
Gonzalez,F.V., Izquierdo,J., Rodriguez,S., McKerrow,J.H. and Hansell,E. Dipeptidyl-alpha,beta-epoxyesters as potent irreversible inhibitors of the cysteine proteases cruzain and rhodesain Bioorg Med Chem Lett (2007) 17, 6697-6700. PubMed Europe PubMed DOI I
Leite,A.C., Moreira,D.R., Cardoso,M.V., Hernandes,M.Z., Alves Pereira,V.R., Silva,R.O., Kiperstok,A.C., Lima,M. and Soares,M.B. Synthesis, cruzain docking, and in vitro studies of aryl-4-oxothiazolylhydrazones against Trypanosoma cruzi ChemMedChem (2007) 2, 1339-1345. PubMed Europe PubMed DOI I
Reis,F.C., Costa,T.F., Sulea,T., Mezzetti,A., Scharfstein,J., Bromme,D., Menard,R. and Lima,A.P. The propeptide of cruzipain - a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F FEBS J (2007) 274, 1224-1234. PubMed Europe PubMed DOI

2006

[YEAR:6-3-2006]Choe,Y., Leonetti,F., Greenbaum,D.C., Lecaille,F., Bogyo,M., Bromme,D., Ellman,J.A. and Craik,C.S. Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities J Biol Chem (6-3-2006) 281, 12824-12832. PubMed Europe PubMed DOI P
Gea,S., Guinazu,N., Pellegrini,A., Carrera Silva,E.A., Giordanengo,L., Cano,R. and Aoki,M.P. Cruzipain, a major Trypanosoma cruzi cystein protease in the host-parasite interplay Inmunologia (2006) 25, 225-238. V

2005

Barboza,M., Duschak,V.G., Fukuyama,Y., Nonami,H., Erra-Balsells,R., Cazzulo,J.J. and Couto,A.S. Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi. Identification of sulfated high-mannose type oligosaccharides FEBS J (2005) 272, 3803-3815. PubMed Europe PubMed DOI
Barr,S.C., Warner,K.L., Kornreic,B.G., Piscitelli,J., Wolfe,A., Benet,L. and McKerrow,J.H. A cysteine protease inhibitor protects dogs from cardiac damage during infection by Trypanosoma cruzi Antimicrob Agents Chemother (2005) 49, 5160-5161. PubMed Europe PubMed DOI PMC EPMC I
[YEAR:15-3-2005]Choe,Y., Brinen,L.S., Price,M.S., Engel,J.C., Lange,M., Grisostomi,C., Weston,S.G., Pallai,P.V., Cheng,H., Hardy,L.W., Hartsough,D.S., McMakin,M., Tilton,R.F., Baldino,C.M. and Craik,C.S. Development of alpha-keto-based inhibitors of cruzain, a cysteine protease implicated in Chagas disease Bioorg Med Chem (15-3-2005) 13, 2141-2156. PubMed Europe PubMed DOI S I
[YEAR:3-1-2005]Fujii,N., Mallari,J.P., Hansell,E.J., Mackey,Z., Doyle,P., Zhou,Y.M., Gut,J., Rosenthal,P.J., McKerrow,J.H. and Guy,R.K. Discovery of potent thiosemicarbazone inhibitors of rhodesain and cruzain Bioorg Med Chem Lett (3-1-2005) 15, 121-123. PubMed Europe PubMed DOI I
[YEAR:9-8-2005]Godat,E., Chowdhury,S., Lecaille,F., Belghazi,M., Purisima,E.O. and Lalmanach,G. Inhibition of a cathepsin L-like cysteine protease by a chimeric propeptide-derived inhibitor Biochemistry (9-8-2005) 44, 10486-10493. PubMed Europe PubMed DOI
[YEAR:1-3-2005]Polticelli,F., Zaini,G., Bolli,A., Antonini,G., Gradoni,L. and Ascenzi,P. Probing the cruzain S2 recognition subsite: a kinetic and binding energy calculation study Biochemistry (1-3-2005) 44, 2781-2789. PubMed Europe PubMed DOI

2004

Aparicio,I.M., Scharfstein,J. and Lima,A.P. A new cruzipain-mediated pathway of human cell invasion by Trypanosoma cruzi requires trypomastigote membranes Infect Immun (2004) 72, 5892-5902. PubMed Europe PubMed DOI PMC EPMC
[YEAR:12-3-2004]Bocedi,A., Gradoni,L., Menegatti,E. and Ascenzi,P. Kinetics of parasite cysteine proteinase inactivation by NO-donors Biochem Biophys Res Commun (12-3-2004) 315, 710-718. PubMed Europe PubMed DOI
Cazzulo,J.J. Cruzipain [ISSN:0-12-079610-4] (2004) 2, 1160-1163. V
Judice,W.A., Puzer,L., Cotrin,S.S., Carmona,A.K., Coombs,G.H., Juliano,L. and Juliano,M.A. Carboxydipeptidase activities of recombinant cysteine peptidases. Cruzain of Trypanosoma cruzi and CPB of Leishmania mexicana Eur J Biochem (2004) 271, 1046-1053. PubMed Europe PubMed DOI P
Rossi,A., Deveraux,Q., Turk,B. and Sali,A. Comprehensive search for cysteine cathepsins in the human genome Biol Chem (2004) 385, 363-372. PubMed Europe PubMed DOI

2003

[YEAR:11-8-2003]Berasain,P., Carmona,C., Frangione,B., Cazzulo,J.J. and Goni,F. Specific cleavage sites on human IgG subclasses by cruzipain, the major cysteine proteinase from Trypanosoma cruzi Mol Biochem Parasitol (11-8-2003) 130, 23-29. PubMed Europe PubMed DOI
Chibale,K. and Musonda,C.C. The synthesis of parasitic cysteine protease and trypanothione reductase inhibitors Curr Med Chem (2003) 10, 1863-1889. PubMed Europe PubMed DOI I
[YEAR:20-10-2003]Chiyanzu,I., Hansell,E., Gut,J., Rosenthal,P.J., McKerrow,J.H. and Chibale,K. Synthesis and evaluation of isatins and thiosemicarbazone derivatives against cruzain, falcipain-2 and rhodesain Bioorg Med Chem Lett (20-10-2003) 13, 3527-3530. PubMed Europe PubMed DOI I
Huang,L., Brinen,L.S. and Ellman,J.A. Crystal structures of reversible ketone-based inhibitors of the cysteine protease cruzain Bioorg Med Chem (2003) 11, 21-29. PubMed Europe PubMed DOI S I

2002

Alvarez,V., Parussini,F., Aslund,L. and Cazzulo,J.J. Expression in insect cells of active mature cruzipain from Trypanosoma cruzi, containing its C-terminal domain Protein Expr Purif (2002) 26, 467-475. PubMed Europe PubMed DOI E
[YEAR:20-6-2002]Du,X., Guo,C., Hansell,E., Doyle,P.S., Caffrey,C.R., Holler,T.P., McKerrow,J.H. and Cohen,F.E. Synthesis and structure-activity relationship study of potent trypanocidal thio semicarbazone inhibitors of the trypanosomal cysteine protease cruzain J Med Chem (20-6-2002) 45, 2695-2707. PubMed Europe PubMed DOI I
[YEAR:21-10-2002]Huang,L. and Ellman,J.A. General solid-phase method to prepare novel cyclic ketone inhibitors of the cysteine protease cruzain Bioorg Med Chem Lett (21-10-2002) 12, 2993-2996. PubMed Europe PubMed DOI I
[YEAR:31-1-2002]Huang,L., Lee,A. and Ellman,J.A. Identification of potent and selective mechanism-based inhibitors of the cysteine protease cruzain using solid-phase parallel synthesis J Med Chem (31-1-2002) 45, 676-684. PubMed Europe PubMed DOI I
Lalmanach,G., Boulange,A., Serveau,C., Lecaille,F., Scharfstein,J., Gauthier,F. and Authie,E. Congopain from Trypanosoma congolense: drug target and vaccine candidate Biol Chem (2002) 383, 739-749. PubMed Europe PubMed DOI
[YEAR:22-2-2002]Lima,A.P., Almeida,P.C., Tersariol,I.L., Schmitz,V., Schmaier,A.H., Juliano,L., Hirata,I.Y., Muller-Esterl,W., Chagas,J.R. and Scharfstein,J. Heparan sulfate modulates kinin release by Trypanosoma cruzi through the activity of cruzipain J Biol Chem (22-2-2002) 277, 5875-5881. PubMed Europe PubMed DOI
[YEAR:3-6-2002]Rodrigues,C.R., Flaherty,T.M., Springer,C., McKerrow,J.H. and Cohen,F.E. CoMFA and HQSAR of acylhydrazide cruzain inhibitors Bioorg Med Chem Lett (3-6-2002) 12, 1537-1541. PubMed Europe PubMed DOI I

2001

Alves,L.C., Melo,R.L., Cezari,M.H., Sanderson,S.J., Mottram,J.C., Coombs,G.H., Juliano,L. and Juliano,M.A. Analysis of the S(2) subsite specificities of the recombinant cysteine proteinases CPB of Leishmania mexicana, and cruzain of Trypanosoma cruzi, using fluorescent substrates containing non-natural basic amino acids Mol Biochem Parasitol (2001) 117, 137-143. PubMed Europe PubMed DOI P
Cazzulo,J.J., Stoka,V. and Turk,V. The major cysteine proteinase of Trypanosoma cruzi: a valid target for chemotherapy of Chagas disease Curr Pharm Des (2001) 7, 1143-1156. PubMed Europe PubMed DOI
de Oliveira,C., Santana,L.A., Carmona,A.K., Cezari,M.H., Sampaio,M.U., Sampaio,C.A.M. and Oliva,M.L.V. Structure of cruzipain/cruzain inhibitors isolated from Bauhinia bauhinioides seeds Biol Chem (2001) 382, 847-852. PubMed Europe PubMed DOI
Judice,W.A.S., Cezari,M.H.S., Lima,A.P.C.A., Scharfstein,J., Chagas,J.R., Tersariol,I.L.S., Juliano,M.A. and Juliano,L. Comparison of the specificity, stability and individual rate constants with respective activation parameters for the peptidase activity of cruzipain and its recombinant form, cruzain, from Trypanosoma cruzi Eur J Biochem (2001) 268, 6578-6586. PubMed Europe PubMed DOI
Lecaille,F., Authie,E., Moreau,T., Serveau,C., Gauthier,F. and Lalmanach,G. Subsite specificity of trypanosomal cathepsin L-like cysteine proteases. Probing the S2 pocket with phenylalanine-derived amino acids Eur J Biochem (2001) 268, 2733-2741. PubMed Europe PubMed DOI
[YEAR:2-11-2001]Lecaille,F., Cotton,J., McKerrow,J.H., Ferrer-Di Martino,M., Boll-Bataille,E., Gauthier,F. and Lalmanach,G. Reversible inhibition of cathepsin L-like proteases by 4-mer pseudopeptides FEBS Lett (2-11-2001) 507, 362-366. PubMed Europe PubMed DOI
[YEAR:25-4-2001]Lima,A.P., dos Reis,F.C., Serveau,C., Lalmanach,G., Juliano,L., Menard,R., Vernet,T., Thomas,D.Y., Storer,A.C. and Scharfstein,J. Cysteine protease isoforms from Trypanosoma cruzi, cruzipain 2 and cruzain, present different substrate preference and susceptibility to inhibitors Mol Biochem Parasitol (25-4-2001) 114, 41-52. PubMed Europe PubMed DOI I
Monteiro,A.C., Abrahamson,M., Lima,A.P., Vannier-Santos,M.A. and Scharfstein,J. Identification, characterization and localization of chagasin, a tight-binding cysteine protease inhibitor in Trypanosoma cruzi J Cell Sci (2001) 114, 3933-3942. PubMed Europe PubMed
[YEAR:22-10-2001]Roush,W.R., Cheng,J., Knapp-Reed,B., Alvarez-Hernandez,A., McKerrow,J.H., Hansell,E. and Engel,J.C. Potent second generation vinyl sulfonamide inhibitors of the trypanosomal cysteine protease cruzain Bioorg Med Chem Lett (22-10-2001) 11, 2759-2762. PubMed Europe PubMed DOI I
Salvati,L., Mattu,M., Polticelli,F., Tiberi,F., Gradoni,L., Venturini,G., Bolognesi,M. and Ascenzi,P. Modulation of the catalytic activity of cruzipain, the major cysteine proteinase from Trypanosoma cruzi, by temperature and pH Eur J Biochem (2001) 268, 3253-3258. PubMed Europe PubMed DOI

2000

[YEAR:15-8-2000]Brinen,L.S., Hansell,E., Cheng,J., Roush,W.R., McKerrow,J.H. and Fletterick,R.J. A target within the target: probing cruzain's P1' site to define structural determinants for the Chagas' disease protease Structure (15-8-2000) 8, 831-840. PubMed Europe PubMed DOI
[YEAR:5-7-2000]Harris,J.L., Backes,B.J., Leonetti,F., Mahrus,S., Ellman,J.A. and Craik,C.S. Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries Proc Natl Acad Sci U S A (5-7-2000) 97, 7754-7759. PubMed Europe PubMed DOI PMC EPMC P
Jacobsen,W., Christians,U. and Benet,L.Z. In vitro evaluation of the disposition of a novel cysteine protease inhibitor Drug Metab Dispos (2000) 28, 1343-1351. PubMed Europe PubMed I
[YEAR:8-12-2000]Roush,W.R., Hernandez,A.A., McKerrow,J.H., Selzer,P.M., Hansell,E. and Engel,J.C. Design, synthesis and evaluation of D-homophenylalanyl epoxysuccinate inhibitors of the trypanosomal cysteine protease cruzain Tetrahedron (8-12-2000) 56, 9747-9762. I
[YEAR:3-3-2000]Stoka,V., Turk,B., McKerrow,J.H., Bjork,I., Cazzulo,J.J. and Turk,V. The high stability of cruzipain against pH-induced inactivation is not dependent on its C-terminal domain FEBS Lett (3-3-2000) 469, 29-32. PubMed Europe PubMed DOI
Yong,V., Schmitz,V., Vannier-Santos,M.A., de Lima,A.P., Lalmanach,G., Juliano,L., Gauthier,F. and Scharfstein,J. Altered expression of cruzipain and a cathepsin B-like target in a Trypanosoma cruzi cell line displaying resistance to synthetic inhibitors of cysteine-proteinases Mol Biochem Parasitol (2000) 109, 47-59. PubMed Europe PubMed DOI

1999

Serveau,C., Lalmanach,G., Hirata,I., Scharfstein,J., Juliano,M.A. and Gauthier,F. Discrimination of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, and mammalian cathepsins B and L, by a pH-inducible fluorogenic substrate of trypanosomal cysteine proteinases Eur J Biochem (1999) 259, 275-280. PubMed Europe PubMed DOI

1998

[YEAR:17-8-1998]Engel,J.C., Doyle,P.S., Hsieh,I. and McKerrow,J.H. Cysteine protease inhibitors cure an experimental Trypanosoma cruzi infection J Exp Med (17-8-1998) 188, 725-734. PubMed Europe PubMed DOI PMC EPMC I
[YEAR:25-9-1998]Lalmanach,G., Lecaille,F., Chagas,J.R., Authie,E., Scharfstein,J., Juliano,M.A. and Gauthier,F. Inhibition of trypanosomal cysteine proteinases by their propeptides J Biol Chem (25-9-1998) 273, 25112-25116. PubMed Europe PubMed DOI
[YEAR:6-10-1998]Roush,W.R., Gonzalez,F.V., McKerrow,J.H. and Hansell,E. Design and synthesis of dipeptidyl alpha',beta'-epoxy ketones, potent irreversible inhibitors of the cysteine protease cruzain Bioorg Med Chem Lett (6-10-1998) 8, 2809-2812. PubMed Europe PubMed DOI I
Scheidt,K.A., Roush,W.R., McKerrow,J.H., Selzer,P.M., Hansell,E. and Rosenthal,P.J. Structure-based design, synthesis and evaluation of conformationally constrained cysteine protease inhibitors Bioorg Med Chem (1998) 6, 2477-2494. PubMed Europe PubMed DOI I

1997

Bevec,T., Stoka,V., Pungercic,G., Cazzulo,J.J. and Turk,V. A fragment of the major histocompatibility complex class II-associated p41 invariant chain inhibits cruzipain, the major cysteine proteinase from Trypanosoma cruzi FEBS Lett (1997) 401, 259-261. PubMed Europe PubMed DOI
Cazzulo,J.J., Stoka,V. and Turk,V. Cruzipain, the major cysteine proteinase from the protozoan parasite Trypanosoma cruzi Biol Chem (1997) 378, 1-10. PubMed Europe PubMed
Del Nery,E., Juliano,M.A., Lima,A.P., Scharfstein,J. and Juliano,L. Kininogenase activity by the major cysteinyl proteinase (cruzipain) from Trypanosoma cruzi J Biol Chem (1997) 272, 25713-25718. PubMed Europe PubMed DOI
Del Nery,E., Juliano,M.A., Meldal,M., Svendsen,I., Scharfstein,J., Walmsley,A. and Juliano,L. Characterization of the substrate specificity of the major cysteine protease (cruzipain) from Trypanosoma cruzi using a portion-mixing combinatorial library and fluorogenic peptides Biochem J (1997) 323, 427-433. PubMed Europe PubMed PMC EPMC
Gillmor,S.A., Craik,C.S. and Fletterick,R.J. Structural determinants of specificity in the cysteine protease cruzain Protein Sci (1997) 6, 1603-1611. PubMed Europe PubMed DOI PMC EPMC S I
Tomas,A.M., Miles,M.A. and Kelly,J.M. Overexpression of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, is associated with enhanced metacyclogenesis Eur J Biochem (1997) 244, 596-603. PubMed Europe PubMed

1996

Cazzulo,J.J., Bravo,M., Raimondi,A., Engstrom,U., Lindeberg,G. and Hellman,U. Hydrolysis of synthetic peptides by cruzipain, the major cysteine proteinase from Trypanosoma cruzi, provides evidence for self-processing and the possibility of more specific substrates for the enzyme Cell Mol Biol (Noisy-le-grand) (1996) 42, 691-696. PubMed Europe PubMed
Metzner,S.I., Sousa,M.C., Hellman,U., Cazzulo,J.J. and Parodi,A.J. The use of UDP-Glc:glycoprotein glucosyltransferase for radiolabeling protein-linked high mannose-type oligosaccharides Cell Mol Biol (Noisy-le-grand) (1996) 42, 631-635. PubMed Europe PubMed
Serveau,C., Lalmanach,G., Juliano,M.A., Scharfstein,J., Juliano,L. and Gauthier,F. Investigation of the substrate specificity of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, through the use of cystatin-derived substrates and inhibitors Biochem J (1996) 313, 951-956. PubMed Europe PubMed PMC EPMC

1995

Fish,W.R., Nkhungulu,Z.M., Muriuki,C.W., Ndegwa,D.M., Lonsdale-Eccles,J.D. and Steyaert,J. Primary structure and partial characterization of a life-cycle-regulated cysteine protease from Trypanosoma (Nannomonas) congolense Gene (1995) 161, 125-128. PubMed Europe PubMed DOI
McGrath,M.E., Eakin,A.E., Engel,J.C., McKerrow,J.H., Craik,C.S. and Fletterick,R.J. The crystal structure of cruzain: A therapeutic target for Chagas' disease J Mol Biol (1995) 247, 251-259. PubMed Europe PubMed DOI S
McKerrow,J.H., McGrath,M.E. and Engel,J.C. The cysteine protease of Trypanosoma cruzi as a model for antiparasite drug design Parasitol Today (1995) 11, 279-282. PubMed Europe PubMed

1994

[YEAR:15-11-1994]Franke de Cazzulo,B.M., Martinez,J., North,M.J., Coombs,G.H. and Cazzulo,J.J. Effects of proteinase inhibitors on the growth and differentiation of Trypanosoma cruzi FEMS Microbiol Lett (15-11-1994) 124, 81-86. PubMed Europe PubMed DOI I
Lima,A.P., Tessier,D.C., Thomas,D.Y., Scharfstein,J., Storer,A.C. and Vernet,T. Identification of new cysteine protease gene isoforms in Trypanosoma cruzi Mol Biochem Parasitol (1994) 67, 333-338. PubMed Europe PubMed DOI
North,M.J. Cysteine endopeptidases of parasitic protozoa Methods Enzymol (1994) 244, 523-539. PubMed Europe PubMed DOI V
Yokoyama-Yasunaka,J.K., Pral,E.M., Oliveira Junior,O.C., Alfieri,S.C. and Stolf,A.M. Trypanosoma cruzi: identification of proteinases in shed components of trypomastigote forms Acta Trop (1994) 57, 307-315. PubMed Europe PubMed DOI

1993

Eakin,A.E., McGrath,M.E., McKerrow,J.H., Fletterick,R.J. and Craik,C.S. Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi J Biol Chem (1993) 268, 6115-6118. PubMed Europe PubMed
Labriola,C., Sousa,M. and Cazzulo,J.J. Purification of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi by affinity chromatography Biol Res (1993) 26, 101-107. PubMed Europe PubMed
Martinez,J., Campetella,O., Frasch,A.C. and Cazzulo,J.J. The reactivity of sera from chagasic patients against different fragments of cruzipain, the major cysteine proteinase from Trypanosoma cruzi, suggests the presence of defined antigenic and catalytic domains Immunol Lett (1993) 35, 191-196. PubMed Europe PubMed DOI

1992

Campetella,O., Henriksson,J., Aslund,L., Frasch,A.C., Pettersson,U. and Cazzulo,J.J. The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes Mol Biochem Parasitol (1992) 50, 225-234. PubMed Europe PubMed DOI
Cazzulo,J.J., Martinez,J., Parodi,A.J., Wernstedt,C. and Hellman,U. On the post-translational modifications at the C-terminal domain of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi FEMS Microbiol Lett (1992) 100, 411-416. PubMed Europe PubMed
Cazzulo,J.J. and Frasch,A.C. SAPA/trans-sialidase and cruzipain: two antigens from Trypanosoma cruzi contain immunodominant but enzymatically inactive domains FASEB J (1992) 6, 3259-3264. PubMed Europe PubMed
Eakin,A.E., Mills,A.A., Harth,G., McKerrow,J.H. and Craik,C.S. The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi J Biol Chem (1992) 267, 7411-7420. PubMed Europe PubMed
[YEAR:15-8-1992]Martinez,J. and Cazzulo,J.J. Anomalous electrophoretic behaviour of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi in relation to its apparent molecular mass FEMS Microbiol Lett (15-8-1992) 74, 225-229. PubMed Europe PubMed

1991

Aslund,L., Henriksson,J., Campetella,O., Frasch,A.C., Pettersson,U. and Cazzulo,J.J. The C-terminal extension of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi Mol Biochem Parasitol (1991) 45, 345-347. PubMed Europe PubMed DOI
McKerrow,J.H. New insights into the structure of a Trypanosoma cruzi protease Parasitol Today (1991) 7, 132-133. PubMed Europe PubMed DOI
Raimondi,A., Wernstedt,C., Hellman,U. and Cazzulo,J.J. Degradation of oxidised insulin A and B chains by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi epimastigotes Mol Biochem Parasitol (1991) 49, 341-344. PubMed Europe PubMed DOI P

1990

Bontempi,E. and Cazzulo,J.J. Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi FEMS Microbiol Lett (1990) 58, 337-341. PubMed Europe PubMed
Cazzulo,J.J., Cazzulo Franke,M.C., Martinez,J. and Franke de Cazzulo,B.M. Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi Biochim Biophys Acta (1990) 1037, 186-191. PubMed Europe PubMed DOI
Greig,S. and Ashall,F. Electrophoretic detection of Trypanosoma cruzi peptidases Mol Biochem Parasitol (1990) 39, 31-37. PubMed Europe PubMed DOI
Murta,A.C., Persechini,P.M., Padron,T., de Souza,W., Guimaraes,J.A. and Scharfstein,J. Structural and functional identification of GP57/51 antigen of Trypanosoma cruzi as a cysteine proteinase Mol Biochem Parasitol (1990) 43, 27-38. PubMed Europe PubMed
Souto-Padron,T., Campetella,O.E., Cazzulo,J.J. and de Souza,W. Cysteine proteinase in Trypanosoma cruzi: immunocytochemical localization and involvement in parasite-host cell interaction J Cell Sci (1990) 96, 485-490. PubMed Europe PubMed

1989

Cazzulo,J.J., Couso,R., Raimondi,A., Wernstedt,C. and Hellman,U. Further characterization and partial amino acid sequence of a cysteine proteinase from Trypanosoma cruzi Mol Biochem Parasitol (1989) 33, 33-41. PubMed Europe PubMed

1984

Bontempi,E., Franke de Cazzulo,B.M., Ruiz,A.M. and Cazzulo,J.J. Purification and some properties of an acidic protease from epimastigotes of Trypanosoma cruzi Comparative Biochemistry and Physiology B (1984) 77, 599-604. DOI

1981

Rangel,H.A., Araujo,P.M., Repka,D. and Costa,M.G. Trypanosoma cruzi: isolation and characterization of a proteinase Exp Parasitol (1981) 52, 199-209. PubMed Europe PubMed

1977

Itow,S. and Camargo,E.P. Proteolytic activites in cell extracts of Trypanosoma cruzi J Protozool (1977) 24, 591-595. PubMed Europe PubMed