Substrates for peptidase M01.010: cytosol alanyl aminopeptidase
Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.
| Substrate | Uniprot | Residue range | Cleavage Site | Cleavage type | Evidence | P4 | P3 | P2 | P1 | P1' | P2' | P3' | P4' | Reference | CutDB | MERNUM |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (Gln)15-Lys-Lys | Gln+(Gln)14-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)16-Lys-Lys | Gln+(Gln)15-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)17-Lys-Lys | Gln+(Gln)16-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)18-Lys-Lys | Gln+(Gln)17-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)19-Lys-Lys | Gln+(Gln)18-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)20-Lys-Lys | Gln+(Gln)19-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)26-Lys-Lys | Gln+(Gln)25-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)27-Lys-Lys | Gln+(Gln)26-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)28-Lys-Lys | Gln+(Gln)27-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)29-Lys-Lys | Gln+(Gln)28-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| (Gln)30-Lys-Lys | Gln+(Gln)29-Lys-Lys | S | - | - | - | Gln | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| Ala-4-methoxy-NHNap | Ala+4-methoxy-NHNap | S | - | - | - | Ala | CBk | - | - | - | Dando & Barrett, 2004 | |||||
| Ala-Ala-Phe-NHMec | Ala+Ala-Phe-NHMec | S | - | - | - | Ala | Ala | Phe | AMC | - | Sharma & Ortwerth, 1986 | |||||
| Ala-NHMec | Ala+NHMec | S | - | - | - | Ala | AMC | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Ala-NHNap | Ala+NHNap | S | - | - | - | Ala | NAP | - | - | - | Hui et al., 1998 | |||||
| Ala-NHPhNO2 | Ala+NHPhNO2 | S | - | - | - | Ala | NAN | - | - | - | Watanabe et al., 1987 | |||||
| Ala-Phe-NHMec | Ala+Phe-NHMec | S | - | - | - | Ala | Phe | AMC | - | - | Sharma & Ortwerth, 1986 | |||||
| Arg-Arg | Arg+Arg | N | - | - | - | Arg | Arg | - | - | - | Johnson & Hersh, 1990 | |||||
| Arg-Leu | Arg+Leu | N | - | - | - | Arg | Leu | - | - | - | Johnson & Hersh, 1990 | |||||
| Arg-NHMec | Arg+NHMec | S | - | - | - | Arg | AMC | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Arg-NHNap | Arg+NHNap | S | - | - | - | Arg | NAP | - | - | - | Hui et al., 1998 | |||||
| Arg-NHPhNO2 | Arg+NHPhNO2 | S | - | - | - | Arg | NAN | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Cys-Lys-Lys-(Gln)20-Lys-Lys | Cys+Lys-Lys-(Gln)20-Lys-Lys | S | - | - | - | Cys | Lys | Lys | Gln | Gln | Bhutani et al., 2007 | |||||
| Glu(EDANS)-(Gln)9-Lys(DABSYL)-Lys | Glu(EDANS)+(Gln)9-Lys(DABSYL)-Lys | S | - | - | - | Glu | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| Gly-NHPhNO2 | Gly+NHPhNO2 | S | - | - | - | Gly | NAN | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Gly-Trp | Gly+Trp | N | - | - | - | Gly | Trp | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Ile-NHNap | Ile+NHNap | S | - | - | - | Ile | NAP | - | - | - | Hui et al., 1998 | |||||
| Leu-Gly | Leu+Gly | N | - | - | - | Leu | Gly | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Leu-Leu | Leu+Leu | N | - | - | - | Leu | Leu | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Leu-Leu-Leu | Leu+Leu-Leu | N | - | - | - | Leu | Leu | Leu | - | - | Sharma & Ortwerth, 1986 | |||||
| Leu-NHMec | Leu+NHMec | S | - | - | - | Leu | AMC | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Leu-NHNap | Leu+NHNap | S | - | - | - | Leu | NAP | - | - | - | Hui et al., 1998 | |||||
| Leu-NHPhNO2 | Leu+NHPhNO2 | S | - | - | - | Leu | NAN | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Leu-Ser | Leu+Ser | N | - | - | - | Leu | Ser | - | - | - | Johnson & Hersh, 1990 | |||||
| Leu-Ser-Phe | Leu+Ser-Phe | N | - | - | - | Leu | Ser | Phe | - | - | Johnson & Hersh, 1990 | |||||
| Leu-Trp | Leu+Trp | N | - | - | - | Leu | Trp | - | - | - | Johnson & Hersh, 1990 | |||||
| Leu-Trp-Met | Leu+Trp-Met | N | - | - | - | Leu | Trp | Met | - | - | Johnson & Hersh, 1990 | |||||
| Lys-(Gln)20-Lys-Lys | Lys+(Gln)20-Lys-Lys | S | - | - | - | Lys | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| Lys-(Gln)30-Lys-Lys | Lys+(Gln)30-Lys-Lys | S | - | - | - | Lys | Gln | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| Lys-Glu(EDANS)-(Gln)9-Lys(DABSYL)-Lys | Lys+Glu(EDANS)-(Gln)9-Lys(DABSYL)-Lys | S | - | - | - | Lys | Glu | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| Lys-Leu | Lys+Leu | N | - | - | - | Lys | Leu | - | - | - | Johnson & Hersh, 1990 | |||||
| Lys-Lys | Lys+Lys | N | - | - | - | Lys | Lys | - | - | - | Johnson & Hersh, 1990 | |||||
| Lys-Lys-(Gln)20-Lys-Lys | Lys+Lys-(Gln)20-Lys-Lys | S | - | - | - | Lys | Lys | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| Lys-Lys-(Gln)30-Lys-Lys | Lys+Lys-(Gln)30-Lys-Lys | S | - | - | - | Lys | Lys | Gln | Gln | Gln | Bhutani et al., 2007 | |||||
| Lys-Lys-Glu(EDANS)-(Gln)9-Lys(DABSYL)-Lys | Lys+Lys-Glu(EDANS)-(Gln)9-Lys(DABSYL)-Lys | S | - | - | - | Lys | Lys | Glu | Gln | Gln | Bhutani et al., 2007 | |||||
| Lys-NHNap | Lys+NHNap | S | - | - | - | Lys | NAP | - | - | - | Hui et al., 1998 | |||||
| Lys-NHPhNO2 | Lys+NHPhNO2 | S | - | - | - | Lys | NAN | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Met-NHNap | Met+NHNap | S | - | - | - | Met | NAP | - | - | - | Hui et al., 1998 | |||||
| Phe-Arg-NHMec | Phe+Arg-NHMec | S | - | - | - | Phe | Arg | AMC | - | - | Sharma & Ortwerth, 1986 | |||||
| Phe-Gly | Phe+Gly | N | - | - | - | Phe | Gky | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Phe-NHMec | Phe+NHMec | S | - | - | - | Phe | AMC | - | - | - | Sharma & Ortwerth, 1986 | |||||
| Phe-NHNap | Phe+NHNap | S | - | - | - | Phe | NAP | - | - | - | Hui et al., 1998 | |||||
| Pro-Phe-Arg-NHMec | Pro+Phe-Arg-NHMec | S | - | - | - | Pro | Phe | Arg | AMC | - | Sharma & Ortwerth, 1986 | |||||
| structural polyprotein | P15480 | 1-512 | peptide-Arg452+Arg-peptide | P | MU | Arg | Ala | Ile | Arg | Arg | Ile | Ala | Val | Irigoyen et al., 2012 | ||
| Tyr-NHNap | Tyr+NHNap | S | - | - | - | Tyr | NAP | - | - | - | Hui et al., 1998 | |||||
| [Leu5]-enkephalin | P01213 | 175-179 | Tyr+Gly-Gly-Phe-Leu-NH2 | N | - | - | - | Tyr | Gly | Gly | Phe | Leu | Hui et al., 1998 | |||
| [Met5]-enkephalin | P01189 | 237-241 | Tyr+Gly-Gly-Phe-Met-NH2 | N | - | - | - | Tyr | Gly | Gly | Phe | Met | Hui et al., 1998 |
