Substrates for peptidase C30.007: coronavirus COVID-19 3C-like peptidase

Summary Alignment Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
cadherin-12	P55289	55-794	peptide-Ser204+Gly-peptide	P	MS	Ser	Ile	Leu	Gln	Gly	Gln	Pro	Tyr
cadherin-6	P55285	54-790	peptide-Ser203+Gly-peptide	P	MS	Ser	Ile	Leu	Gln	Gly	Gln	Pro	Tyr
E3 ubiquitin-protein ligase BRE1A	Q5VTR2	1-975	peptide-Gln521+Ser-peptide	P	NT	Ala	Leu	Leu	Gln	Ser	Gln	Ser	Ser	Zhang et al., 2021
eukaryotic translation initiation factor 4 gamma 1	Q04637	1-1599	peptide-Gln658+Gly-peptide	P	MS	Thr	Arg	Leu	Gln	Gly	Ile	Asn	Cys	Koudelka et al., 2020
eukaryotic translation initiation factor 4 gamma 1	Q04637	1-1599	peptide-Gln1127+Gln-peptide	P	MS	Ser	Ala	Leu	Gln	Gln	Ala	Val	Pro	Koudelka et al., 2020
gasdermin-D	P57764	1-484	peptide-Gly194+Glu-peptide	P	NT	Cys	Leu	Gln	Gly	Glu	Gly	Gln	Gly
NACHT, LRR and PYD domains-containing protein 1	Q9C000	1-1473	peptide-Gly334+Ala-peptide	P	NT	Ile	Leu	Gln	Gly	Ala	Ala	Gly	Ile
NACHT, LRR and PYD domains-containing protein 12	P59046	1-1061	peptide-Gln241+Gly-peptide	P	CS	Lys	Leu	Phe	Gln	Gly	Arg	Phe	Asp	Moustaqil et al., 2020
NACHT, LRR and PYD domains-containing protein 12	P59046	1-1061	peptide-Gln938+Ala-peptide	P	CS	Val	Val	Leu	Gln	Ala	Asn	His	Asn	Moustaqil et al., 2020
NACHT, LRR and PYD domains-containing protein 12	P59046	1-1061	peptide-Ala939+Asn-peptide	P	NT	Val	Leu	Gln	Ala	Asn	His	Asn	Leu
neurogenic locus notch homolog protein 1	P46531	19-2555	peptide-Gln2315+Ser-peptide	P	NT	Ser	Arg	Leu	Gln	Ser	Gly	Met	Val
nF-kappa-B essential modulator	Q9Y6K9	1-419	peptide-Gln231+Val-peptide	P	MS	Ala	Gln	Leu	Gln	Val	Ala	Tyr	His	Koudelka et al., 2020
nF-kappa-B essential modulator	Q9Y6K9	1-419	peptide-Val232+Ala-peptide	P	NT	Gln	Leu	Gln	Val	Ala	Tyr	His	Gln
optineurin	Q96CV9	1-577	peptide-Gln151+Ala-peptide	P	MS	Val	Arg	Leu	Gln	Ala	Glu	Lys	Ala	Koudelka et al., 2020
optineurin	Q96CV9	1-577	peptide-Gln165+Leu-peptide	P	MS	Ser	Glu	Leu	Gln	Leu	Lys	Leu	Asn	Koudelka et al., 2020
polyubiquitin-B	P0CG47	1-229	peptide-Gly228+Cys	P	NT	Leu	Arg	Gly	Gly	Cys	'-'	'-'	'-'
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gln3263+Ser-peptide	P	CS	Ala	Val	Leu	Gln	Ser	Gly	Phe	Arg
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gln3569+Ser-peptide	P	CS	Val	Thr	Phe	Gln	Ser	Ala	Val	Lys
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gln3859+Ser-peptide	P	CS	Ala	Thr	Val	Gln	Ser	Lys	Met	Ser
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gln3942+Ala-peptide	P	CS	Ala	Thr	Leu	Gln	Ala	Ile	Ala	Ser
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gln4140+Asn-peptide	P	CS	Val	Lys	Leu	Gln	Asn	Asn	Glu	Leu
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gln4253+Ala-peptide	P	CS	Val	Arg	Leu	Gln	Ala	Gly	Asn	Ala
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gln4392+Ser-peptide	P	CS	Pro	Met	Leu	Gln	Ser	Ala	Asp	Ala
tGF-beta-activated kinase 1 and MAP3K7-binding protein 1	Q15750	1-504	peptide-Gln132+Ser-peptide	P	CS	Ala	Ser	Leu	Gln	Ser	Gln	Leu	Pro	Moustaqil et al., 2020
tGF-beta-activated kinase 1 and MAP3K7-binding protein 1	Q15750	1-504	peptide-Gln444+Ser-peptide	P	CS	Leu	Thr	Leu	Gln	Ser	Thr	Asn	Thr	Moustaqil et al., 2020
tGF-beta-activated kinase 1 and MAP3K7-binding protein 1	Q15750	1-504	peptide-Ser445+Thr-peptide	P	NT	Thr	Leu	Gln	Ser	Thr	Asn	Thr	His
tGF-beta-activated kinase 1 and MAP3K7-binding protein 1	Q15750	1-504	peptide-Ser133+Gln-peptide	P	NT	Ser	Leu	Gln	Ser	Gln	Leu	Pro	Glu
ubiquitin-like protein ISG15	P05161	2-165	peptide-Gly157+Gly-peptide	P	NT	Leu	Arg	Gly	Gly	Gly	Thr	Glu	Pro