Substrates for peptidase A01.068: necepsin-2

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
Abz-Ile-Glu-Phe-Nph-Arg-Leu-NH2			Abz-Ile-Glu-Phe+Nph-Arg-Leu-NH2	S		Abz	Ile	Glu	Phe	Nph	Arg	Leu	NH2	Williamson et al., 2003
hemoglobin subunit alpha	P69905	2-142	peptide-Thr42+Tyr-peptide	N	MS	Thr	Thr	Lys	Thr	Tyr	Phe	Pro	His	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Gly23+Glu-Peptide	N	MS	Ala	His	Ala	Gly	Glu	Tyr	Gly	Ala	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Phe34+Leu-Peptide	N	MS	Glu	Arg	Met	Phe	Leu	Ser	Phe	Pro	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Phe47+Asp-Peptide	N	MS	Phe	Pro	His	Phe	Asp	Leu	Ser	His	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Leu49+Ser-Peptide	N	MS	His	Phe	Asp	Leu	Ser	His	Gly	Ser	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Ala70+Val-Peptide	N	MS	Leu	Thr	Asn	Ala	Val	Ala	His	Val	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Leu81+Ser-Peptide	N	MS	Pro	Asn	Ala	Leu	Ser	Ala	Leu	Ser	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Ser82+Ala-Peptide	N	MS	Asn	Ala	Leu	Ser	Ala	Leu	Ser	Asp	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Ala83+Leu-Peptide	N	MS	Ala	Leu	Ser	Ala	Leu	Ser	Asp	Leu	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Leu87+His-Peptide	N	MS	Leu	Ser	Asp	Leu	His	Ala	His	Lys	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Asn98+Phe-Peptide	N	MS	Asp	Pro	Val	Asn	Phe	Lys	Leu	Leu	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Phe99+Lys-Peptide	N	MS	Pro	Val	Asn	Phe	Lys	Leu	Leu	Ser	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Lys100+Leu-Peptide	N	MS	Val	Asn	Phe	Lys	Leu	Leu	Ser	His	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Leu101+Leu-Peptide	N	MS	Asn	Phe	Lys	Leu	Leu	Ser	His	Cys	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Leu110+Ala-Peptide	N	MS	Leu	Val	Thr	Leu	Ala	Ala	His	Leu	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Phe118+Thr-Peptide	N	MS	Pro	Ala	Glu	Phe	Thr	Pro	Ala	Val	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Thr119+Pro-Peptide	N	MS	Ala	Glu	Phe	Thr	Pro	Ala	Val	His	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-His123+Ala-Peptide	N	MS	Pro	Ala	Val	His	Ala	Ser	Leu	Asp	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Phe129+Leu-Peptide	N	MS	Leu	Asp	Lys	Phe	Leu	Ala	Ser	Val	Ranjit et al., 2009
Hemoglobin subunit alpha	P69905	1-142	Peptide-Leu130+Ala-Peptide	N	MS	Asp	Lys	Phe	Leu	Ala	Ser	Val	Ser	Ranjit et al., 2009
hemoglobin subunit beta	P68871	2-147	peptide-Phe42+Phe-peptide	N	MS	Thr	Gln	Arg	Phe	Phe	Glu	Ser	Phe	Ranjit et al., 2009
hemoglobin subunit beta	P68871	2-147	peptide-Val114+Leu-peptide	N	MS	Leu	Val	Cys	Val	Leu	Ala	His	His	Ranjit et al., 2009
hemoglobin subunit beta	P68871	2-147	peptide-Leu115+Ala-peptide	N	MS	Val	Cys	Val	Leu	Ala	His	His	Phe	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Lys9+Ser-Peptide	N	MS	Pro	Glu	Glu	Lys	Ser	Ala	Val	Thr	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Leu15+Trp-Peptide	N	MS	Val	Thr	Ala	Leu	Trp	Gly	Lys	Val	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Trp16+Gly-Peptide	N	MS	Thr	Ala	Leu	Trp	Gly	Lys	Val	Asn	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Val21+Asp-Peptide	N	MS	Lys	Val	Asn	Val	Asp	Glu	Val	Gly	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Asp22+Glu-Peptide	N	MS	Val	Asn	Val	Asp	Glu	Val	Gly	Gly	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Glu23+Val-Peptide	N	MS	Asn	Val	Asp	Glu	Val	Gly	Gly	Glu	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Ala28+Leu-Peptide	N	MS	Gly	Gly	Glu	Ala	Leu	Gly	Arg	Leu	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Leu29+Gly-Peptide	N	MS	Gly	Glu	Ala	Leu	Gly	Arg	Leu	Leu	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Leu32+Leu-Peptide	N	MS	Leu	Gly	Arg	Leu	Leu	Val	Val	Tyr	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Phe42+Phe-Peptide	N	MS	Thr	Gln	Arg	Phe	Phe	Glu	Ser	Phe	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Ser73+Asp-Peptide	N	MS	Gly	Ala	Phe	Ser	Asp	Gly	Leu	Ala	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Phe86+Ala-Peptide	N	MS	Lys	Gly	Thr	Phe	Ala	Thr	Leu	Ser	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Cys113+Val-Peptide	N	MS	Val	Leu	Val	Cys	Val	Leu	Ala	His	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Val114+Leu-Peptide	N	MS	Leu	Val	Cys	Val	Leu	Ala	His	His	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Leu115+Ala-Peptide	N	MS	Val	Cys	Val	Leu	Ala	His	His	Phe	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Phe123+Thr-Peptide	N	MS	Gly	Lys	Glu	Phe	Thr	Pro	Pro	Val	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Ala130+Tyr-Peptide	N	MS	Val	Gln	Ala	Ala	Tyr	Gln	Lys	Val	Ranjit et al., 2009
Hemoglobin subunit beta	P68871	1-147	Peptide-Ala143+His-Peptide	N	MS	Asn	Ala	Leu	Ala	His	Lys	Tyr	His	Ranjit et al., 2009
Leu-Asp-Lys-Phe-Phe-Ala-Ala-Val			Leu-Asp-Lys-Phe+Phe-Ala-Ala-Val	S		Leu	Asp	Lys	Phe	Phe	Ala	Ala	Val	Williamson et al., 2002
Leu-Asp-Lys-Phe-Phe-Ala-Ser-Val			Leu-Asp-Lys-Phe+Phe-Ala-Ser-Val	S		Leu	Asp	Lys	Phe	Phe	Ala	Ser	Val	Williamson et al., 2002
Mca-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2			Mca-Gly-Lys-Pro-Ile-Leu-Phe+Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2	S		Pro	Ile	Leu	Phe	Phe	Arg	Leu	Lyd	Yasuda et al., 1999