Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 6 |
cathepsin L |
3-19 |
N |
|
MS |
Biniossek et al., 2011 |
| 6 |
cathepsin S |
3-19 |
N |
|
MS |
Biniossek et al., 2011 |
| 6 |
meprin beta subunit |
3-19 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 22 |
glutamyl endopeptidase I |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
HIV-1 retropepsin |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
cathepsin S |
20-43 |
N |
|
MS |
Biniossek et al., 2011 |
| 26 |
matrix metallopeptidase-2 |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
glutamyl endopeptidase I |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 33 |
elastase-2 |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 37 |
cathepsin L |
20-46 |
N |
|
MS |
Biniossek et al., 2011 |
| 46 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 58 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 62 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 63 |
matrix metallopeptidase-2 |
59-81 |
N |
|
MS |
Schilling & Overall, 2008 |
| 77 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 81 |
chymotrypsin A (cattle-type) |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 82 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 83 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
elastase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 89 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 89 |
cathepsin G |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 90 |
cathepsin G |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 103 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 108 |
cathepsin K |
103-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 108 |
glutamyl endopeptidase I |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 109 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
matrix metallopeptidase-2 |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
glutamyl endopeptidase I |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 112 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 112 |
cathepsin G |
103-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 113 |
elastase-2 |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
HIV-1 retropepsin |
103-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
cathepsin S |
104-122 |
N |
|
MS |
Biniossek et al., 2011 |
| 114 |
caspase-3 |
103-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 121 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 122 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 137 |
cathepsin G |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 138 |
matrix metallopeptidase-2 |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 140 |
cathepsin S |
123-152 |
N |
|
MS |
Biniossek et al., 2011 |
| 144 |
glutamyl endopeptidase I |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 154 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 162 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 173 |
matrix metallopeptidase-2 |
172-183 |
N |
|
MS |
Schilling & Overall, 2008 |
| 174 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 183 |
chymotrypsin A (cattle-type) |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 186 |
matrix metallopeptidase-2 |
177-198 |
N |
|
MS |
Schilling & Overall, 2008 |
| 198 |
glutamyl endopeptidase I |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 224 |
glutamyl endopeptidase I |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 227 |
matrix metallopeptidase-2 |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 231 |
matrix metallopeptidase-2 |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 241 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 251 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 252 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 262 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 282 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 297 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 309 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 318 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 323 |
peptidyl-Lys metallopeptidase |
1-445 |
N |
|
MS |
|
| 324 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 335 |
peptidyl-Lys metallopeptidase |
1-445 |
N |
|
MS |
|
| 336 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 339 |
matrix metallopeptidase-2 |
336-350 |
N |
|
MS |
Schilling & Overall, 2008 |
| 349 |
peptidyl-Lys metallopeptidase |
1-445 |
N |
|
MS |
|
| 350 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 359 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 362 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 380 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 390 |
trypsin 1 |
1-445 |
N |
|
MS |
Schilling & Overall, 2008 |
| 392 |
lysyl endopeptidase (bacteria) |
1-445 |
N |
|
MS |
|
