Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 21 |
unknown peptidase |
1-803 |
P |
release of a signal peptide |
NT |
Gevaert et al., 2003 |
| 28 |
unknown peptidase |
|
P |
|
MS |
Mahrus et al., 2008 |
| 51 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 55 |
HIV-1 retropepsin |
51-67 |
N |
|
MS |
Schilling & Overall, 2008 |
| 67 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 75 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 87 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 95 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 97 |
lysyl endopeptidase (bacteria) |
1-803 |
N |
|
MS |
|
| 102 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 105 |
elastase-2 |
102-116 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 116 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 145 |
cathepsin G |
142-156 |
N |
|
MS |
Schilling & Overall, 2008 |
| 156 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 162 |
matrix metallopeptidase-2 |
158-173 |
N |
|
MS |
Schilling & Overall, 2008 |
| 173 |
glutamyl endopeptidase I |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 174 |
chymotrypsin A (cattle-type) |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 203 |
HIV-1 retropepsin |
177-214 |
N |
|
MS |
Schilling & Overall, 2008 |
| 203 |
matrix metallopeptidase-2 |
177-214 |
N |
|
MS |
Schilling & Overall, 2008 |
| 203 |
cathepsin G |
177-214 |
N |
|
MS |
Schilling & Overall, 2008 |
| 214 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 251 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 252 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 264 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 265 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 284 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 302 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 318 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 327 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 331 |
meprin alpha subunit |
22-803 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 331 |
meprin beta subunit |
22-803 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 331 |
LAST_MAM peptidase (Limulus-type) |
328-348 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 335 |
HIV-1 retropepsin |
328-348 |
N |
|
MS |
Schilling & Overall, 2008 |
| 341 |
matrix metallopeptidase-2 |
328-356 |
N |
|
MS |
Schilling & Overall, 2008 |
| 347 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 348 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 356 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 371 |
matrix metallopeptidase-2 |
364-384 |
N |
|
MS |
Schilling & Overall, 2008 |
| 383 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 384 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 395 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 403 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 404 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 415 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 420 |
LAST_MAM peptidase (Limulus-type) |
417-728 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 428 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 485 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 520 |
matrix metallopeptidase-2 |
511-530 |
N |
|
MS |
Schilling & Overall, 2008 |
| 530 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 559 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 578 |
cathepsin L |
562-587 |
N |
|
MS |
Biniossek et al., 2011 |
| 592 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 602 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 670 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 671 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 681 |
peptidyl-Lys metallopeptidase |
1-803 |
N |
|
MS |
|
| 682 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 712 |
matrix metallopeptidase-2 |
708-724 |
N |
|
MS |
Schilling & Overall, 2008 |
| 724 |
trypsin 1 |
22-803 |
N |
|
MS |
Schilling & Overall, 2008 |
| 752 |
granzyme B (Homo sapiens-type) |
22-803 |
N |
|
MS |
Van Damme et al., 2010 |
| 787 |
caspase-6 |
22-803 |
P |
|
|
Klaiman et al., 2008 |
| 801 |
caspase-6 |
22-803 |
P |
|
|
Klaiman et al., 2008 |
