Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-732 |
P |
|
NT |
<%Agarwal et al., 2012[]%> |
| 41 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 46 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 50 |
cathepsin L |
47-58 |
N |
|
MS |
Biniossek et al., 2011 |
| 57 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 60 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 68 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 69 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 73 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 74 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 77 |
astacin |
74-87 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 77 |
meprin alpha subunit |
74-87 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 83 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 87 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 90 |
cathepsin L |
88-100 |
N |
|
MS |
Biniossek et al., 2011 |
| 90 |
cathepsin S |
88-100 |
N |
|
MS |
Biniossek et al., 2011 |
| 99 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 100 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 112 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 163 |
cathepsin L |
154-173 |
N |
|
MS |
Biniossek et al., 2011 |
| 175 |
caspase-6 |
2-732 |
P |
|
MS |
Klaiman et al., 2008 |
| 175 |
granzyme B (Homo sapiens-type) |
2-732 |
P |
|
AB |
Hostetter et al., 2007 |
| 185 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 188 |
cathepsin L |
186-201 |
N |
|
MS |
Biniossek et al., 2011 |
| 189 |
cathepsin S |
186-201 |
N |
|
MS |
Biniossek et al., 2011 |
| 191 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 201 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 203 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 215 |
meprin alpha subunit |
2-732 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 223 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 227 |
caspase-6 |
2-732 |
P |
|
MS |
Klaiman et al., 2008 |
| 233 |
caspase-6 |
2-732 |
P |
|
MS |
Klaiman et al., 2008 |
| 250 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 264 |
caspase-6 |
2-732 |
P |
|
MS |
Klaiman et al., 2008 |
| 274 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 280 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 281 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 282 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 283 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 291 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 292 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 299 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 313 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 314 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 317 |
cathepsin L |
315-327 |
N |
|
MS |
Biniossek et al., 2011 |
| 317 |
cathepsin S |
315-327 |
N |
|
MS |
Biniossek et al., 2011 |
| 326 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 327 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 367 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 386 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 400 |
trypsin 1 |
2-732 |
N |
|
|
Nemoto et al., 1997 |
| 407 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 418 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 424 |
cathepsin L |
420-435 |
N |
|
MS |
Biniossek et al., 2011 |
| 424 |
astacin |
421-436 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 424 |
meprin alpha subunit |
421-436 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 425 |
cathepsin S |
420-434 |
N |
|
MS |
Biniossek et al., 2011 |
| 430 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 435 |
lysyl endopeptidase (bacteria) |
1-732 |
N |
|
MS |
|
| 436 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 443 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 464 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 467 |
cathepsin L |
465-478 |
N |
|
MS |
Biniossek et al., 2011 |
| 467 |
cathepsin S |
465-478 |
N |
|
MS |
Biniossek et al., 2011 |
| 478 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 488 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 498 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 499 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 510 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 545 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 546 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 550 |
cathepsin S |
547-559 |
N |
|
MS |
Biniossek et al., 2011 |
| 557 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 558 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 559 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 573 |
trypsin 1 |
2-732 |
N |
|
|
Nemoto et al., 1997 |
| 576 |
meprin alpha subunit |
2-732 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 576 |
meprin beta subunit |
2-732 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 614 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 615 |
trypsin 1 |
2-732 |
N |
|
|
Nemoto et al., 1997 |
| 620 |
trypsin 1 |
2-732 |
N |
|
|
Nemoto et al., 1997 |
| 630 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 631 |
trypsin 1 |
2-732 |
N |
|
MS |
Schilling & Overall, 2008 |
| 633 |
meprin alpha subunit |
2-732 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 635 |
LAST_MAM peptidase (Limulus-type) |
632-647 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 638 |
meprin alpha subunit |
635-647 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 639 |
meprin alpha subunit |
636-647 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 639 |
granzyme B (Homo sapiens-type) |
2-732 |
P |
|
MS |
Hostetter et al., 2007 |
| 648 |
peptidyl-Lys metallopeptidase |
1-732 |
N |
|
MS |
|
| 657 |
lysyl endopeptidase (bacteria) |
1-732 |
N |
|
MS |
|
| 670 |
matrix metallopeptidase-11 |
2-732 |
N |
|
|
Kleifeld et al., 2010 |
| 677 |
cathepsin L |
661-687 |
N |
|
MS |
Biniossek et al., 2011 |
| 677 |
cathepsin S |
661-687 |
N |
|
MS |
Biniossek et al., 2011 |
| 701 |
caspase-6 |
2-732 |
P |
|
MS |
Klaiman et al., 2008 |
| 701 |
granzyme B (Homo sapiens-type) |
2-732 |
P |
|
MS |
Hostetter et al., 2007 |
| 702 |
caspase-6 |
2-732 |
P |
|
MS |
Klaiman et al., 2008 |
| 722 |
caspase-6 |
2-732 |
P |
|
MS |
Klaiman et al., 2008 |
| 722 |
granzyme B (Homo sapiens-type) |
2-732 |
N |
|
MS |
Van Damme et al., 2010 |
