Summary for peptidase S01.151: trypsin 1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Nametrypsin 1
Other namespseudotrypsin, psi-trypsin, trypsin 7 (Mus musculus), trypsin III (cationic) (Rattus norvegicus), trypsin 1 (Sus scrofa), trypsin 1 (tetrapod, non-Homo sapiens)
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.151
Holotypetrypsin 1 (Bos taurus), Uniprot accession P00760 (peptidase unit: 24-246), MERNUM MER0000024
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00281
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.4
EnzymologyBRENDA database
Proteolytic eventsCutDB database (29 cleavages)
PhysiologyMajor role in intestinal digestion of food proteins in mammals, and also in the activation of zymogens of other digestive peptidases.
Pathways KEGGInfluenza A
KEGGNeuroactive ligand-receptor interaction
KEGGPancreatic secretion
KEGGProtein digestion and absorption
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Trypsin_1
TREEFAMhttp://www.treefam.org/family/TF331065
PANTHERhttp://www.pantherdb.org/panther/familyList.do?searchType=basic&fieldName=all&listType=6&fieldValue=PTHR24264:SF39
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/KRScissile bond-/-/-/- (based on 21961 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1407 1565 1711 10 1479 1640 1665 1412
Pro 1268 971 1372 3 74 1053 1330 1393
Ala 1786 1807 1806 21 1994 1870 1805 1678
Val 1366 1602 1454 9 1871 1760 1610 1517
Leu 2012 2565 2389 20 2396 2825 2010 2042
Ile 1179 1343 1261 7 1532 1489 1227 1241
Met 448 525 482 4 579 534 428 428
Phe 759 946 864 32 872 931 951 759
Tyr 566 685 632 19 701 654 579 494
Trp 177 205 222 4 195 204 155 232
Ser 1385 1358 1432 6 1452 1360 1449 1502
Thr 1110 1197 1099 8 1449 1198 1253 1305
Cys 252 295 304 2 225 298 317 280
Asn 952 845 878 8 971 752 825 838
Gln 1078 974 1100 7 845 973 1130 1186
Asp 1522 1149 833 7 1234 1079 1417 1576
Glu 2080 1565 1834 8 1684 1181 2133 2382
Lys 1271 1203 1216 12499 1178 1065 747 690
Arg 832 707 671 9279 666 637 481 496
His 430 410 372 8 526 407 368 396
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Sus scrofa recombinant broad broad broad R - - - - bbbR AMC Furlong et al., 2002
Homo sapiens recombinant nd nd nd R - - - - R ACC Harris et al., 2000
Bos taurus wild type - L P R H L P - xLPR+HLPx Abz-Dnp Alves et al., 2007
Homo sapiens recombinant Q K G R - - - - QKGR+xxxx Abz-Tyr(NO2) Janssen et al., 2004
Homo sapiens recombinant - M/K/Q R/K - R/S V/I/R G/P/S/D - xMRx+RVGx Abz-EDDnp Bianchini et al., 2002
Bos taurus wild type - K A/broad K - - - - xKAK+xxxx ACC-Dabcyl Sun et al., 2007
Relevant inhibitors antipain, benzamidine, dabigatran, DCI, DFP, DPC423, DX-9065a, E64, leupeptin, MUGB, MUTMAC, nafamostat mesilate, NPGB, PMSF, sepimostat mesilate, TLCK, tranexamic acid
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
2210010C04Rik 6:B1 ENSMUSG00000029882 67373 MGI:1914623