Summary for peptidase S01.151: trypsin 1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Nametrypsin 1
Other namespseudotrypsin, psi-trypsin, trypsin 7 (Mus musculus), trypsin III (cationic) (Rattus norvegicus), trypsin 1 (Sus scrofa), trypsin 1 (tetrapod, non-Homo sapiens)
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.151
Holotypetrypsin 1 (Bos taurus), Uniprot accession P00760 (peptidase unit: 24-246), MERNUM MER0000024
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00281
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.4
EnzymologyBRENDA database
Proteolytic eventsCutDB database (29 cleavages)
PhysiologyMajor role in intestinal digestion of food proteins in mammals, and also in the activation of zymogens of other digestive peptidases.
Pathways KEGGInfluenza A
KEGGNeuroactive ligand-receptor interaction
KEGGPancreatic secretion
KEGGProtein digestion and absorption
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Trypsin_1
TREEFAMhttp://www.treefam.org/family/TF331065
PANTHERhttp://www.pantherdb.org/panther/familyList.do?searchType=basic&fieldName=all&listType=6&fieldValue=PTHR24264:SF39
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/KRScissile bond-/-/-/- (based on 21933 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1406 1564 1711 10 1476 1640 1663 1408
Pro 1264 970 1370 3 74 1053 1329 1392
Ala 1785 1806 1802 21 1994 1870 1803 1678
Val 1363 1600 1451 9 1870 1758 1607 1517
Leu 2012 2561 2388 20 2391 2819 2009 2039
Ile 1176 1340 1259 7 1532 1488 1224 1239
Met 448 525 482 4 578 534 428 426
Phe 758 946 864 32 871 929 947 759
Tyr 565 685 632 19 700 654 579 494
Trp 177 205 222 4 194 204 154 232
Ser 1383 1353 1430 6 1448 1358 1447 1497
Thr 1109 1197 1096 8 1449 1195 1251 1305
Cys 252 294 303 2 225 298 316 280
Asn 952 844 877 8 971 750 824 838
Gln 1077 974 1098 7 844 970 1129 1183
Asp 1519 1143 832 7 1234 1079 1417 1574
Glu 2077 1563 1831 8 1678 1180 2132 2379
Lys 1270 1202 1215 12492 1176 1061 747 689
Arg 829 707 670 9258 665 635 480 494
His 430 410 371 8 525 407 366 396
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Sus scrofa recombinant broad broad broad R - - - - bbbR AMC Furlong et al., 2002
Homo sapiens recombinant nd nd nd R - - - - R ACC Harris et al., 2000
Bos taurus wild type - L P R H L P - xLPR+HLPx Abz-Dnp Alves et al., 2007
Homo sapiens recombinant Q K G R - - - - QKGR+xxxx Abz-Tyr(NO2) Janssen et al., 2004
Homo sapiens recombinant - M/K/Q R/K - R/S V/I/R G/P/S/D - xMRx+RVGx Abz-EDDnp Bianchini et al., 2002
Bos taurus wild type - K A/broad K - - - - xKAK+xxxx ACC-Dabcyl Sun et al., 2007
Relevant inhibitors antipain, benzamidine, dabigatran, DCI, DFP, DPC423, DX-9065a, E64, leupeptin, MUGB, MUTMAC, nafamostat mesilate, NPGB, PMSF, sepimostat mesilate, TLCK, tranexamic acid
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
2210010C04Rik 6:B1 ENSMUSG00000029882 67373 MGI:1914623