$VAR1 = undef;

Summary for peptidase M27.002: bontoxilysin

Names
MEROPS Name	bontoxilysin
Other names	BoNT/A, BoNT/B, BoNT/C, BoNT/D, BoNT/E, BoNT/F, BoNT/G, BoNT/X, botox A, botulinum neurotoxin type A, botulinum neurotoxin type B, botulinum neurotoxin type C, botulinum neurotoxin type D, botulinum neurotoxin type E, botulinum neurotoxin type F, botulinum neurotoxin type G, botulinum neurotoxin type H, botulinum toxin, Dysport, myobloc

Domain architecture

MEROPS Classification
Classification	Clan MA >> Subclan MA(E) >> Family M27 >> Subfamily (none) >> M27.002
Holotype	bontoxilysin (Clostridium botulinum), Uniprot accession P10844 (peptidase unit: 118-450), MERNUM MER0001176
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Metallo
Peplist	Included in the Peplist with identifier PL00244
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.69
Enzymology	BRENDA database
Proteolytic events	CutDB database (8 cleavages)
Pharmaceutical relevance	As Botox, has therapeutic use for local paralysis of neuromuscular function, as in strabismus (Naumann & Jankovic, 2004).
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	e/a/dsq/qrka/-/etk/k (based on 10 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	2	0	0	0	0	0	0	0
Pro	0	0	0	0	0	0	0	0
Ala	1	3	0	1	3	1	0	0
Val	0	0	0	0	0	1	0	0
Leu	0	0	0	0	1	1	1	1
Ile	0	2	0	0	2	0	0	0
Met	0	0	0	0	0	2	0	1
Phe	0	0	0	0	2	0	0	0
Tyr	0	0	0	0	0	0	0	1
Trp	0	0	0	0	0	0	0	0
Ser	0	0	3	0	0	1	1	2
Thr	0	2	0	0	0	1	3	0
Cys	0	0	0	0	0	0	0	0
Asn	0	1	1	0	0	0	0	0
Gln	2	0	2	4	0	0	0	0
Asp	1	1	3	0	0	0	0	0
Glu	3	0	0	0	0	2	3	1
Lys	0	0	1	2	1	1	2	4
Arg	1	1	0	3	1	0	0	0
His	0	0	0	0	0	0	0	0