$VAR1 = undef;

Summary for peptidase M12.221: ADAMTS4 peptidase

Names
MEROPS Name	ADAMTS4 peptidase
Other names	ADAMTS4 g.p. (Homo sapiens), aggrecanase-1, ADAMTS-2 (Tang), ADAMTS-4, ADMP-1, KIAA0688 protein

Domain architecture

MEROPS Classification
Classification	Clan MA >> Subclan MA(M) >> Family M12 >> Subfamily B >> M12.221
Holotype	ADAMTS4 peptidase (Homo sapiens), Uniprot accession O75173 (peptidase unit: 213-428), MERNUM MER0005101
History	Identifier created: MEROPS 3.21 (12 May 1999)

Activity
Catalytic type	Metallo
Peplist	Included in the Peplist with identifier PL00196
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.82
Enzymology	BRENDA database
Proteolytic events	CutDB database (53 cleavages)
Activity status	human: active (Hughes et al., 2004) mouse: active (by similarity)
Knockout	Mice in which the ADAMTS4 gene was distrupted exhibited no gross or histologic abnormalities, and indicate that, in the mouse, ADAMTS-4 is not the primary enzyme responsible for aggrecan degradation at the TEGE(373-374)ARGS site Glasson et al., 2004.
Pharmaceutical relevance	Like ADAM-TS5, ADAM-TS4 is a potential drug target for the treatment of osteoarthritis (Malfait et al., 2002).
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/ADAMTS4
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/-/-/ea/-/ga/- (based on 506 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	47	35	9	30	22	25	155	50
Pro	15	31	19	15	0	7	2	21
Ala	41	70	99	73	99	43	102	57
Val	46	30	15	3	72	45	8	37
Leu	45	55	73	14	94	72	8	37
Ile	30	21	13	2	17	49	6	26
Met	10	10	38	17	18	19	3	10
Phe	11	55	13	22	43	9	5	14
Tyr	14	42	10	50	25	13	6	15
Trp	3	1	9	1	0	1	1	5
Ser	29	20	35	26	30	20	42	40
Thr	39	31	13	19	21	38	18	15
Cys	3	1	9	2	4	6	2	7
Asn	24	17	9	11	5	18	17	21
Gln	26	5	57	26	19	27	27	36
Asp	23	17	14	11	7	5	21	24
Glu	29	34	44	159	7	37	34	38
Lys	26	16	6	2	1	6	6	6
Arg	37	11	13	12	17	56	38	23
His	7	4	8	11	5	10	5	24