$VAR1 = undef;

Summary for peptidase C30.005: SARS coronavirus picornain 3C-like peptidase

Names
MEROPS Name	SARS coronavirus picornain 3C-like peptidase
Other names	M(pro) (SARS coronavirus), SARS virus 3C-like peptidase, SARS virus chymotrypsin-like cysteine peptidase, SARS-CoV main proteinase, SARS-CoV M(pro), severe acute respiratory syndrome coronavirus main protease

Domain architecture

MEROPS Classification
Classification	Clan PA >> Subclan PA(C) >> Family C30 >> Subfamily (none) >> C30.005
Holotype	SARS coronavirus picornain 3C-like peptidase, Uniprot accession P0C6U8 (peptidase unit: 3241-3546), MERNUM MER0022793
History	Identifier created: MEROPS 6.5 (22 December 2003)

Activity
Catalytic type	Cysteine
Peplist	Included in the Peplist with identifier PL00434
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.69
Enzymology	BRENDA database
Structure	Active in vitro only as the homodimer (Fan et al., 2003).
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	Lav/-/GL/QGasg/-/-/a (based on 27 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	0	1	13	13	2	3	1	0
Pro	2	0	0	0	0	0	0	0
Ala	6	1	0	0	5	2	1	4
Val	4	3	1	0	1	1	0	1
Leu	13	2	10	0	0	1	0	1
Ile	0	0	0	0	0	1	0	0
Met	0	0	1	0	0	0	1	0
Phe	0	1	2	0	0	0	4	0
Tyr	0	1	0	0	0	0	1	0
Trp	0	1	0	0	0	0	0	0
Ser	0	1	0	0	5	1	0	2
Thr	2	5	0	0	0	0	0	0
Cys	0	0	0	0	0	0	0	0
Asn	0	1	0	0	1	1	2	1
Gln	0	2	0	14	0	0	1	0
Asp	0	0	0	0	0	0	1	0
Glu	0	0	0	0	0	1	2	0
Lys	0	3	0	0	0	3	0	2
Arg	0	3	0	0	0	0	0	2
His	0	1	0	0	0	0	0	1

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
West Nile virus	recombinant	L	K/R/L/broad	G	G	-	-	-	-	LKGG	ACC	Drag et al., 2008
SARS coronavirus	recombinant	V/T/A/S	V/W/K	L	H/Q	-	-	-	-	VVLH	ACC	Goetz et al., 2007