Substrates for peptidase C30.005: SARS coronavirus picornain 3C-like peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
Ac-Leu-Ala-Gly-Gly-AFC			Ac-Leu-Ala-Gly-Gly+AFC	S		Leu	Ala	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Arg-Gly-Gly-AFC			Ac-Leu-Arg-Gly-Gly+AFC	S		Leu	Arg	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Asn-Gly-Gly-AFC			Ac-Leu-Asn-Gly-Gly+AFC	S		Leu	Asn	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Gln-Gly-Gly-AFC			Ac-Leu-Gln-Gly-Gly+AFC	S		Leu	Gln	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Gly-Gly-Gly-AFC			Ac-Leu-Gly-Gly-Gly+AFC	S		Leu	Gly	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-His-Gly-Gly-AFC			Ac-Leu-His-Gly-Gly+AFC	S		Leu	His	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Leu-Gly-Gly-AFC			Ac-Leu-Leu-Gly-Gly+AFC	S		Leu	Leu	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Lys-Gly-Gly-AFC			Ac-Leu-Lys-Gly-Gly+AFC	S		Leu	Lys	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Nle-Gly-Gly-AFC			Ac-Leu-Nle-Gly-Gly+AFC	S		Leu	Nle	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Phe-Gly-Gly-AFC			Ac-Leu-Phe-Gly-Gly+AFC	S		Leu	Phe	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Ser-Gly-Gly-AFC			Ac-Leu-Ser-Gly-Gly+AFC	S		Leu	Ser	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Trp-Gly-Gly-AFC			Ac-Leu-Trp-Gly-Gly+AFC	S		Leu	Trp	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Leu-Tyr-Gly-Gly-AFC			Ac-Leu-Tyr-Gly-Gly+AFC	S		Leu	Tyr	Gly	Gly	AFC	-	-	-	Drag et al., 2008
nF-kappa-B essential modulator	Q9Y6K9	1-419	peptide-Gln231+Val-peptide	P	MS	Ala	Gln	Leu	Gln	Val	Ala	Tyr	His	Koudelka et al., 2020
P1/P2 cleavage site peptide			Thr-Ser-Ala-Val-Leu-Gln+Ser-Gly-Phe-Arg-Lys-NH2	N		Ala	Val	Leu	Gln	Ser	Gly	Phe	Arg	Fan et al., 2004
P10/P11 cleavage site peptide			Thr-Phe-Thr-Arg-Leu-Gln+Ser-Leu-Glu-Asn-Val	N		Thr	Arg	Leu	Gln	Ser	Leu	Glu	Asn	Fan et al., 2004
P11/P12 cleavage site peptide			Phe-Tyr-Pro-Lys-Leu-Gln+Ala-Ser-Gln-Ala-Trp	N		Pro	Lys	Leu	Gln	Ala	Ser	Gln	Ala	Fan et al., 2004
P2/P3 cleavage site peptide			Ser-Gly-Val-Thr-Phe-Gln+Gly-Lys-Phe-Lys-Lys	N		Val	Thr	Phe	Gln	Gly	Lys	Phe	Lys	Fan et al., 2004
P3/P4 cleavage site peptide			Lys-Val-Ala-Thr-Val-Gln+Ser-Lys-Met-Ser-Asp	N		Ala	Thr	Val	Gln	Ser	Lys	Met	Ser	Fan et al., 2004
P4/P5 cleavage site peptide			Asn-Arg-Ala-Thr-Leu-Gln+Ala-Ile-Ala-Ser-Glu	N		Ala	Thr	Leu	Gln	Ala	Ile	Ala	Ser	Fan et al., 2004
P5/P6 cleavage site peptide			Ser-Ala-Val-Lys-Leu-Gln+Asn-Asn-Glu-Leu-Ser	N		Val	Lys	Leu	Gln	Asn	Asn	Glu	Leu	Fan et al., 2004
P6/P7 cleavage site peptide			Ala-Thr-Val-Arg-Leu-Gln+Ala-Gly-Asn-Ala-Thr	N		Val	Arg	Leu	Gln	Ala	Gly	Asn	Ala	Fan et al., 2004
P7/P8 cleavage site peptide			Arg-Glu-Pro-Leu-Met-Gln+Ser-Ala-Asp-Ala-Ser	N		Pro	Leu	Met	Gln	Ser	Ala	Asp	Ala	Fan et al., 2004
P8/P9 cleavage site peptide			Pro-His-Thr-Val-Leu-Gln+Ala-Val-Gly-Ala-Cys	N		Thr	Val	Leu	Gln	Ala	Val	Gly	Ala	Fan et al., 2004
P9/P10 cleavage site peptide			Asn-Val-Ala-Thr-Leu-Gln+Ala-Glu-Asn-Val-Thr	N		Ala	Thr	Leu	Gln	Ala	Glu	Asn	Val	Fan et al., 2004
SARS coronavirus papain-like peptidase	P59641	1-7073	peptide-Gln3240+Ser-peptide	P		Ala	Val	Leu	Gln	Ser	Gly	Phe	Arg	Hsu et al., 2005
SARS coronavirus papain-like peptidase	P59641	1-7073	peptide-Gln3546+Gly-peptide	P		Val	Thr	Phe	Gln	Gly	Lys	Phe	Lys	Hsu et al., 2005