Summary for peptidase M12.005: procollagen C-peptidase

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Nameprocollagen C-peptidase
Other namesbone morphogenetic protein 1, BMP1 g.p. (Homo sapiens), mammalian tolloid, mTLD, procollagen C-proteinase, XTLD g.p. (Xenopus laevis)
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(M) >> Family M12 >> Subfamily A >> M12.005
Holotypeprocollagen C-peptidase (Homo sapiens), Uniprot accession P13497 (peptidase unit: 127-317), MERNUM MER0001113
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00176
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.19
EnzymologyBRENDA database
Proteolytic eventsCutDB database (19 cleavages)
Activity statushuman: active (Kessler, 2004)
mouse: active (Scott et al., 2000)
PhysiologyCleaves C-terminal propeptide in maturation of type 1 procollagen.
KnockoutMice homozygous null for procollagen C-proteinase and mammalian tolloid-like 1 protein have been described (Pappano et al., 2003).
Pharmaceutical relevanceConsidered to be a drug target for fibrosis (Robinson et al., 2003).
Other databases TREEFAMhttp://www.treefam.org/family/TF314351
PANTHERhttp://www.pantherdb.org/panther/familyList.do?searchType=basic&fieldName=all&listType=6&fieldValue=PTHR43978:SF3
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/m/q/gansScissile bondD/deq/pa/- (based on 41 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 2 1 1 10 0 2 1 7
Pro 2 3 1 0 0 0 14 3
Ala 1 3 1 9 1 3 9 5
Val 4 1 4 0 0 1 1 0
Leu 3 2 2 1 0 0 1 3
Ile 1 2 1 0 0 0 0 1
Met 0 11 3 0 0 0 1 1
Phe 5 4 0 0 0 0 1 0
Tyr 6 7 2 0 0 0 0 2
Trp 0 1 0 0 0 0 1 0
Ser 5 3 4 6 1 2 1 3
Thr 1 0 1 0 1 2 4 1
Cys 1 0 1 0 0 0 0 0
Asn 1 0 0 6 0 0 2 5
Gln 2 1 13 4 3 6 0 4
Asp 2 0 0 1 35 13 1 0
Glu 2 1 1 2 0 10 4 2
Lys 0 0 0 1 0 0 0 1
Arg 3 1 5 1 0 2 0 2
His 0 0 1 0 0 0 0 1
Relevant inhibitors marimastat
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
BMP1 8p21 ENSG00000168487 649 BMP1 112264
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Bmp1 14:D1 ENSMUSG00000022098 12153 MGI:88176