Substrates for peptidase S08.034: subtilisin BPN'

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
Ac-Ala-Ala-Ala-OMe			Ac-Ala-Ala-Ala-OMe	S		Ac	Ala	Ala	Ala	OMe	-	-	-	Narahashi & Yoda, 1977
alpha-2-macroglobulin	P01023	24-1474	peptide-Arg719+Leu-peptide	P		Gly	His	Ala	Arg	Leu	Val	His	Val	Mortensen et al., 1981
alpha-2-macroglobulin	P01023	24-1474	peptide-Leu720+Val-peptide	P		His	Ala	Arg	Leu	Val	His	Val	Glu	Mortensen et al., 1981
Antitrypsin	P22922	17-392	Peptide-Tyr361+Val-Peptide	P	NT	Ser	Lys	Val	Tyr	Val	Arg	Pro	Pro	Sasaki et al., 1990
Glucoamylase	P69328	25-640	Peptide-Val494+Ala-Peptide	N	NT	Pro	Ser	Ile	Val	Ala	Thr	Gly	Gly	Stoffer et al., 1993
Glucoamylase	P69328	25-640	Peptide-Ser467+Ser-Peptide	N	NT	Thr	Ser	Ala	Ser	Ser	Val	Pro	Gly	Stoffer et al., 1993
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Val	Glu	Ala	Leu	Tyr	Leu	Val	Cya	Kobayashi et al., 2007	24154
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr+Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Glu	Ala	Leu	Tyr	Leu	Val	Cya	Gly	Kobayashi et al., 2007	24155
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser+His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Leu	Cya	Gly	Ser	His	Leu	Val	Glu	Kobayashi et al., 2007	24152
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln+His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Phe	Val	Asn	Gln	His	Leu	Cya	Gly	Kobayashi et al., 2007	24151
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe+Tyr-Thr-Pro-Lys-Ala	N	MS	Arg	Gly	Phe	Phe	Tyr	Thr	Pro	Lys	Kobayashi et al., 2007
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu+Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Ala	Leu	Tyr	Leu	Val	Cya	Gly	Glu	Koma et al., 2007	24156
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu+Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala	N	MS	Gly	Ser	His	Leu	Val	Glu	Ala	Leu	Koma et al., 2007	24153
insulin B-chain (oxidized)	P01317	25-54	Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr+Thr-Pro-Lys-Ala	N	MS	Gly	Phe	Phe	Tyr	Thr	Pro	Lys	Ala	Koma et al., 2007	24157
subtilisin BPN' propeptide			peptide-Glu53+Lys-peptide	N		Thr	Leu	Asn	Glu	Lys	Ala	Val	Lys	Kojima et al., 1997
subtilisin BPN' propeptide			peptide-Thr17+Met-peptide	N		Phe	Lys	Gln	Thr	Met	Ser	Thr	Met	Kojima et al., 1997
subtilisin BPN' propeptide			peptide-Met21+Ser-peptide	N		Met	Ser	Thr	Met	Ser	Ala	Ala	Lys	Kojima et al., 1997