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Literature for peptidase S08.034: subtilisin BPN'

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, P Specificity, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Zhou,C., Kong,Y., Zhang,N., Zhang,X., Qin,W., Zhang,L., Zhang,H., Yang,G. and Lu,F.<br>Transcriptomic analysis of Bacillus licheniformis 2709 reveals the molecular mechanism of alkaline protease biosynthesis regulated by the DegS/DegU two-component system<br>Int J Biol Macromol (2025) 306, 140868-140868. PubMed  Europe PubMed DOI
    2. 2022
  2. Ishida,K., Shimizu,M., Wakasugi,A., Matsui,Y., Nakamura,A. and Kojima,S.<br>Development of a novel peptide inhibitor of subtilisin BPN'<br>FEBS Open Bio (2022) 12, 2057-2064. PubMed  Europe PubMed DOI  PMC  EPMC  I
    3. 2021
  3. Chen,Y., Toth,E.A., Ruan,B., Choi,E.J., Simmerman,R., Chen,Y., He,Y., Wang,R., Godoy-Ruiz,R., King,H., Custer,G., Travis Gallagher,D., Rozak,D.A., Solomon,M., Muro,S., Weber,D.J., Orban,J., Fuerst,T.R. and Bryan,P.N.<br>Engineering subtilisin proteases that specifically degrade active RAS<br>Commun Biol (2021) 4, 299-299. PubMed  Europe PubMed DOI  PMC  EPMC
  4. Weeks,A.M., Byrnes,J.R., Lui,I. and Wells,J.A.<br>Mapping proteolytic neo-N termini at the surface of living cells<br>Proc Natl Acad Sci U S A (2021) 118 PubMed  Europe PubMed DOI
    5. 2020
  5. Che,Z., Cao,X., Chen,G. and Liang,Z.<br>An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)<br>BMC Biotechnol (2020) 20, 63-63. PubMed  Europe PubMed DOI  PMC  EPMC
    6. 2015
  6. Iqbal,I., Aftab,M.N., Afzal,M., Ur-Rehman,A., Aftab,S., Zafar,A., Ud-Din,Z., Khuharo,A.R., Iqbal,J. and Ul-Haq,I.<br>Purification and characterization of cloned alkaline protease gene of Geobacillus stearothermophilus<br>J Basic Microbiol (2015) 55, 160-171. PubMed  Europe PubMed DOI  E
    7. 2012
  7. Graycar,T.P., Bott,R.R., Power,S.D. and Estell,D.A.<br>Subtilisins<br>[ISSN:978-0-12-407742-3] (2012) 3, 3148-3155. DOI
    8. 2011
  8. Jo,H.D., Lee,H.A., Jeong,S.J. and Kim,J.H.<br>Purification and characterization of a major fibrinolytic enzyme from Bacillus amyloliquefaciens MJ5-41 isolated from meju<br>J Microbiol Biotechnol (2011) 21, 1166-1173. PubMed  Europe PubMed
  9. Niu,Q., Tian,Y., Zhang,L., Xu,X., Niu,X., Xia,Z., Lei,L., Zhang,K.Q. and Huang,X.<br>Overexpression of the key virulence proteases Bace16 and Bae16 in Bacillus nematocida B16 to improve its nematocidal activity<br>J Mol Microbiol Biotechnol (2011) 21, 130-137. PubMed  Europe PubMed DOI
    10. 2010
  10. Huang,W., Tian,B.Y., Guo,J., Cai,W.L. and Huang,J.Z.<br>Production of an in vitro-derived deletion mutation of Brevibacillus laterosporus by constructing a homology-driven integration vector<br>Curr Microbiol (2010) 61, 401-406. PubMed  Europe PubMed DOI
    11. 2009
  11. Gallagher,T., Ruan,B., London,M., Bryan,M.A. and Bryan,P.N.<br>Structure of a switchable subtilisin complexed with a substrate and with the activator azide<br>Biochemistry (2009) 48, 10389-10394. PubMed  Europe PubMed DOI  S
  12. Tian,B., Huang,W., Huang,J., Jiang,X. and Qin,L.<br>Investigation of protease-mediated cuticle-degradation of nematodes by using an improved immunofluorescence-localization method<br>J Invertebr Pathol (2009) 101, 143-146. PubMed  Europe PubMed DOI
    13. 2007
  13. Knight,Z.A., Garrison,J.L., Chan,K., King,D.S. and Shokat,K.M.<br>A remodelled protease that cleaves phosphotyrosine substrates<br>J Am Chem Soc (2007) 129, 11672-11673. PubMed  Europe PubMed DOI
  14. Lian,L.H., Tian,B.Y., Xiong,R., Zhu,M.Z., Xu,J. and Zhang,K.Q.<br>Proteases from Bacillus: a new insight into the mechanism of action for rhizobacterial suppression of nematode populations<br>Lett Appl Microbiol (2007) 45, 262-269. PubMed  Europe PubMed DOI
  15. Niu,Q., Huang,X., Zhang,L., Lian,L., Li,Y., Li,J., Yang,J. and Zhang,K.<br>Functional identification of the gene bace16 from nematophagous bacterium Bacillus nematocida<br>Appl Microbiol Biotechnol (2007) 75, 141-148. PubMed  Europe PubMed DOI
  16. Tian,B., Yang,J., Lian,L., Wang,C., Li,N. and Zhang,K.Q.<br>Role of an extracellular neutral protease in infection against nematodes by Brevibacillus laterosporus strain G4<br>Appl Microbiol Biotechnol (2007) 74, 372-380. PubMed  Europe PubMed DOI
    17. 2006
  17. Taniguchi,M., Atiwetin,P., Hirai,T., Itoh,M., Harada,S., Hara,S. and Kamei,K.<br>Interaction of subtilisin BPN' and recombinant fungal protease inhibitor F from silkworm with substituted P1 site residues<br>Biosci Biotechnol Biochem (2006) 70, 1262-1264. PubMed  Europe PubMed DOI
  18. [YEAR:3-8-2006]Tian,B., Li,N., Lian,L., Liu,J., Yang,J. and Zhang,K.Q.<br>Cloning, expression and deletion of the cuticle-degrading protease BLG4 from nematophagous bacterium Brevibacillus laterosporus G4<br>Arch Microbiol (3-8-2006) 186, 297-305. PubMed  Europe PubMed DOI
    19. 2005
  19. Huang,X., Tian,B., Niu,Q., Yang,J., Zhang,L. and Zhang,K.<br>An extracellular protease from Brevibacillus laterosporus G4 without parasporal crystals can serve as a pathogenic factor in infection of nematodes<br>Res Microbiol (2005) 156, 719-727. PubMed  Europe PubMed DOI
    20. 2004
  20. [YEAR:15-7-2004]Choi,N.S., Chang,K.T., Jae Maeng,P. and Kim,S.H.<br>Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4<br>FEMS Microbiol Lett (15-7-2004) 236, 325-331. PubMed  Europe PubMed DOI
  21. Ganz,P.J., Bauer,M.D., Sun,Y., Fieno,A.M., Grant,R.A., Correa,P.E., Laskowski,M., Jr. and Saunders,C.W.<br>Stabilized variant of Streptomyces subtilisin inhibitor and its use in stabilizing subtilisin BPN'<br>Protein Eng Des Sel (2004) 17, 333-339. PubMed  Europe PubMed DOI
  22. Graycar,T.P., Ballinger,M.D. and Wells,J.A.<br>Subtilisins<br>[ISSN:0-12-079610-4] (2004) 2, 1786-1792.  V
    23. 2003
  23. Day,R.M., Thalhauser,C.J., Sudmeier,J.L., Vincent,M.P., Torchilin,E.V., Sanford,D.G., Bachovchin,C.W. and Bachovchin,W.W.<br>Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN' by NMR<br>Protein Sci (2003) 12, 794-810. PubMed  Europe PubMed DOI  PMC  EPMC
    24. 2002
  24. Almog,O., Gallagher,D.T., Ladner,J.E., Strausberg,S., Alexander,P., Bryan,P. and Gilliland,G.L.<br>Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'<br>J Biol Chem (2002) 277, 27553-27558. PubMed  Europe PubMed DOI  S
    25. 2001
  25. Alexander,P.A., Ruan,B. and Bryan,P.N.<br>Cation-dependent stability of subtilisin<br>Biochemistry (2001) 40, 10634-10639. PubMed  Europe PubMed DOI
  26. Kojima,S., Yanai,H. and Miura,K.<br>Accelerated refolding of subtilisin BPN' by tertiary-structure-forming mutants of its propeptide<br>J Biochem (2001) 130, 471-474. PubMed  Europe PubMed
    27. 2000
  27. Yokota,M., Tsuda,I. and Tatsumi,N.<br>Availability of Nagarse for DNA analysis as a substitute for proteinase K<br>J Clin Lab Anal (2000) 14, 97-100. PubMed  Europe PubMed DOI
    28. 1999
  28. Dinakarpandian,D., Shenoy,B.C., Hilvert,D., McRee,D.E., McTigue,M. and Carey,P.R.<br>Electric fields in active sites: substrate switching from null to strong fields in thiol- and selenol-subtilisins<br>Biochemistry (1999) 38, 6659-6667. PubMed  Europe PubMed DOI
    29. 1998
  29. Almog,O., Gallagher,T., Tordova,M., Hoskins,J., Bryan,P. and Gilliland,G.L.<br>Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain<br>Proteins (1998) 31, 21-32. PubMed  Europe PubMed DOI  S
    30. 1997
  30. Huang,H.W., Chen,W.C., Wu,C.Y., Yu,H.C., Lin,W.Y., Chen,S.T. and Wang,K.T.<br>Kinetic studies of the inhibitory effects of propeptides subtilisin BPN' and Carlsberg to bacterial serine proteases<br>Protein Eng (1997) 10, 1227-1233. PubMed  Europe PubMed
    31. 1996
  31. Ballinger,M.D., Tom,J. and Wells,J.A.<br>Furilisin: a variant of subtilisin BPN' engineered for cleaving tribasic substrates<br>Biochemistry (1996) 35, 13579-13585. PubMed  Europe PubMed DOI
  32. Gallagher,T., Oliver,J., Bott,R., Betzel,C. and Gilliland,G.L.<br>Subtilisin BPN' at 1.6 A resolution: analysis for discrete disorder and comparison of crystal forms<br>Acta Crystallogr D Biol Crystallogr (1996) 52, 1125-1135. PubMed  Europe PubMed DOI  S
    33. 1995
  33. [YEAR:17-10-1995]Ballinger,M.D., Tom,J. and Wells,J.A.<br>Designing subtilisin BPN' to cleave substrates containing dibasic residues<br>Biochemistry (17-10-1995) 34, 13312-13319. PubMed  Europe PubMed DOI
  34. Gallagher,T., Gilliland,G., Wang,L. and Bryan,P.<br>The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'<br>Structure (1995) 3, 907-914. PubMed  Europe PubMed
  35. Genov,N., Filippi,B., Dolashka,P., Wilson,K.S. and Betzel,C.<br>Stability of subtilisins and related proteinases (subtilases)<br>Int J Pept Protein Res (1995) 45, 391-400. PubMed  Europe PubMed
  36. Strausberg,S.L., Alexander,P.A., Gallagher,D.T., Gilliland,G.L., Barnett,B.L. and Bryan,P.N.<br>Directed evolution of a subtilisin with calcium-independent stability<br>Biotechnology (N Y) (1995) 13, 669-673. PubMed  Europe PubMed
    37. 1994
  37. [YEAR:11-1-1994]Rheinnecker,M., Eder,J., Pandey,P.S. and Fersht,A.R.<br>Variants of subtilisin BPN' with altered specificity profiles<br>Biochemistry (11-1-1994) 33, 221-225. PubMed  Europe PubMed
    38. 1993
  38. Eder,J., Rheinnecker,M. and Fersht,A.R.<br>Folding of subtilisin BPN': role of the pro-sequence<br>J Mol Biol (1993) 233, 293-304. PubMed  Europe PubMed DOI
  39. [YEAR:13-12-1993]Eder,J., Rheinnecker,M. and Fersht,A.R.<br>Hydrolysis of small peptide substrates parallels binding of chymotrypsin inhibitor 2 for mutants of subtilisin BPN'<br>FEBS Lett (13-12-1993) 335, 349-352. PubMed  Europe PubMed DOI
  40. [YEAR:21-12-1993]Jackson,S.E. and Fersht,A.R.<br>Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'<br>Biochemistry (21-12-1993) 32, 13909-13916. PubMed  Europe PubMed  I
  41. Stoffer,B., Frandsen,T.P., Busk,P.K., Schneider,P., Svendsen,I. and Svensson,B.<br>Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger<br>Biochem J (1993) 292, 197-202. PubMed  Europe PubMed  PMC  EPMC  P
    42. 1992
  42. [YEAR:1-9-1992]Braxton,S. and Wells,J.A.<br>Incorporation of a stabilizing Ca(2+)-binding loop into subtilisin BPN'<br>Biochemistry (1-9-1992) 31, 7796-7801. PubMed  Europe PubMed
  43. Bryan,P., Alexander,P., Strausberg,S., Schwarz,F., Lan,W., Gilliland,G. and Gallagher,D.T.<br>Energetics of folding subtilisin BPN'<br>Biochemistry (1992) 31, 4937-4945. PubMed  Europe PubMed DOI
  44. Gron,H., Meldal,M. and Breddam,K.<br>Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching<br>Biochemistry (1992) 31, 6011-6018. PubMed  Europe PubMed DOI
    45. 1991
  45. [YEAR:25-6-1991]Braxton,S. and Wells,J.A.<br>The importance of a distal hydrogen bonding group in stabilizing the transition state in subtilisin BPN'<br>J Biol Chem (25-6-1991) 266, 11797-11800. PubMed  Europe PubMed
  46. [YEAR:25-6-1991]Carter,P., Abrahmsen,L. and Wells,J.A.<br>Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'<br>Biochemistry (25-6-1991) 30, 6142-6148. PubMed  Europe PubMed
  47. Siezen,R.J., de Vos,W.M., Leunissen,J.A. and Dijkstra,B.W.<br>Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases<br>Protein Eng (1991) 4, 719-737. PubMed  Europe PubMed
  48. Takeuchi,Y., Satow,Y., Nakamura,K.T. and Mitsui,Y.<br>Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8 A resolution<br>J Mol Biol (1991) 221, 309-325. PubMed  Europe PubMed
  49. Takeuchi,Y., Noguchi,S., Satow,Y., Kojima,S., Kumagai,I., Miura,K., Nakamura,K.T. and Mitsui,Y.<br>Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor)<br>Protein Eng (1991) 4, 501-508. PubMed  Europe PubMed  S
    50. 1990
  50. Carter,P. and Wells,J.A.<br>Functional interaction among catalytic residues in subtilisin BPN'<br>Proteins (1990) 7, 335-342. PubMed  Europe PubMed DOI
  51. Erwin,C.R., Barnett,B.L., Oliver,J.D. and Sullivan,J.F.<br>Effects of engineered salt bridges on the stability of subtilisin BPN'<br>Protein Eng (1990) 4, 87-97. PubMed  Europe PubMed  S
    52. 1989
  52. Carter,P., Nilsson,B., Burnier,J.P., Burdick,D. and Wells,J.A.<br>Engineering subtilisin BPN' for site-specific proteolysis<br>Proteins (1989) 6, 240-248. PubMed  Europe PubMed DOI
  53. [YEAR:5-9-1989]Pantoliano,M.W., Whitlow,M., Wood,J.F., Dodd,S.W., Hardman,K.D., Rollence,M.L. and Bryan,P.N.<br>Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding<br>Biochemistry (5-9-1989) 28, 7205-7213. PubMed  Europe PubMed
    54. 1988
  54. [YEAR:5-6-1988]Bott,R., Ultsch,M., Kossiakoff,A., Graycar,T., Katz,B. and Power,S.<br>The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation<br>J Biol Chem (5-6-1988) 263, 7895-7906. PubMed  Europe PubMed  S
  55. McPhalen,C.A. and James,M.N.<br>Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo<br>Biochemistry (1988) 27, 6582-6598. PubMed  Europe PubMed  S
    56. 1987
  56. [YEAR:24-7-1987]Carter,P. and Wells,J.A.<br>Engineering enzyme specificity by "substrate-assisted catalysis"<br>Science (24-7-1987) 237, 394-399. PubMed  Europe PubMed
    57. 1986
  57. [YEAR:1-2-1986]Bromme,D., Peters,K., Fink,S. and Fittkau,S.<br>Enzyme-substrate interactions in the hydrolysis of peptide substrates by thermitase, subtilisin BPN', and proteinase K<br>Arch Biochem Biophys (1-2-1986) 244, 439-446. PubMed  Europe PubMed
    58. 1985
  58. Mitsui,Y., Akagawa,H., Onishi,H., Itoh,S., Nakamura,K.T. and Iitaka,Y.<br>Crystal structure of subtilisin complexed with its trapped substrate Streptomyces subtilisin inhibitor - protein-protein interaction and evolution of serine proteinases and their proteinaceous inhibitors<br>J Biosci (1985) 8, 481-489. DOI  S
  59. Nedkov,P., Oberthur,W. and Braunitzer,G.<br>Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN', Carlsberg and amylosacchariticus<br>Biol Chem Hoppe Seyler (1985) 366, 421-430. PubMed  Europe PubMed
    60. 1984
  60. [YEAR:15-9-1984]Hirono,S., Akagawa,H., Mitsui,Y. and Iitaka,Y.<br>Crystal structure at 2.6 A resolution of the complex of subtilisin BPN' with streptomyces subtilisin inhibitor<br>J Mol Biol (15-9-1984) 178, 389-414. PubMed  Europe PubMed  S
    61. 1983
  61. Wells,J.A., Ferrari,E., Henner,D.J., Estell,D.A. and Chen,E.Y.<br>Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis<br>Nucleic Acids Res (1983) 11, 7911-7925. PubMed  Europe PubMed  PMC  EPMC
    62. 1977
  62. Priest,F.G.<br>Extracellular enzyme synthesis in the genus Bacillus<br>Bacteriol Rev (1977) 41, 711-753. PubMed  Europe PubMed  PMC  EPMC
    63. 1975
  63. [YEAR:25-9-1975]Matthews,D.A., Alden,R.A., Birktoft,J.J., Freer,S.T. and Kraut,J.<br>X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo). A model for the catalytic transition state<br>J Biol Chem (25-9-1975) 250, 7120-7126. PubMed  Europe PubMed  S  I
    64. 1972
  64. Adams,K.R.<br>Investigations on the chemical reactivity of the carboxyl groups in subtilisin type Novo<br>C R Trav Lab Carlsberg [Chim] (1972) 38, 481-498.
  65. [YEAR:27-3-1972]Drenth,J., Hol,W.G., Jansonius,J.N. and Koekoek,R.<br>Subtilisin Novo. The three-dimensional structure and its comparison with subtilisin BPN'<br>Eur J Biochem (27-3-1972) 26, 177-181. PubMed  Europe PubMed  S
  66. [YEAR:20-6-1972]Robertus,J.D., Alden,R.A., Birktoft,J.J., Kraut,J., Powers,J.C. and Wilcox,P.E.<br>An X-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN'<br>Biochemistry (20-6-1972) 11, 2439-2449. PubMed  Europe PubMed  S  I
    67. 1971
  67. Alden,R.A., Birktoft,J.J., Kraut,J., Robertus,J.D. and Wright,C.S.<br>Atomic coordinates for subtilisin BPN' (or Novo)<br>Biochem Biophys Res Commun (1971) 45, 337-344. PubMed  Europe PubMed  S
  68. [YEAR:22-1-1971]Robertus,J.D., Alden,R.A. and Kraut,J.<br>On the identity of subtilisins BPN' and Novo<br>Biochem Biophys Res Commun (22-1-1971) 42, 334-339. PubMed  Europe PubMed
    69. 1970
  69. Morihara,K. and Oka,T.<br>Subtilisin BPN': inactivation by chloromethyl ketone derivates of peptide substrates<br>Arch Biochem Biophys (1970) 138, 526-531. PubMed  Europe PubMed
  70. Ottesen,M. and Svendsen,A.<br>[11] The subtilisins<br>Methods Enzymol (1970) 19, 199-215. DOI  V
    71. 1969
  71. [YEAR:18-1-1969]Wright,C.S., Alden,R.A. and Kraut,J.<br>Structure of subtilisin BPN' at 2.5 angstrom resolution<br>Nature (18-1-1969) 221, 235-242. PubMed  Europe PubMed DOI  S
    72. 1966
  72. Smith,E.L., Markland,F.S., Kasper,C.B., DeLange,R.J., Landon,M. and Evans,W.H.<br>The complete amino acid sequence of two types of subtilisin, BPN' and Carlsberg<br>J Biol Chem (1966) 241, 5974-5976. PubMed  Europe PubMed
    73. 1960
  73. Sanger,F. and Shaw,D.C.<br>Amino-acid sequence about the reactive serine of a proteolytic enzyme from Bacillus subtilis<br>Nature (1960) 187, 872-873. PubMed  Europe PubMed DOI  I
    74. 1956
  74. Okunuki,K., Matsubara,H., Nishimura,S. and Hagihara,B.<br>Specificity of crystalline bacterial proteinase<br>J Biochem (1956) 43, 857-866.  P

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