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Literature for peptidase S08.034: subtilisin BPN'

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, P Specificity, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Zhou,C., Kong,Y., Zhang,N., Zhang,X., Qin,W., Zhang,L., Zhang,H., Yang,G. and Lu,F.
    Transcriptomic analysis of Bacillus licheniformis 2709 reveals the molecular mechanism of alkaline protease biosynthesis regulated by the DegS/DegU two-component system
    Int J Biol Macromol (2025) 306, 140868-140868. PubMed  Europe PubMed DOI
    2. 2022
  2. Ishida,K., Shimizu,M., Wakasugi,A., Matsui,Y., Nakamura,A. and Kojima,S.
    Development of a novel peptide inhibitor of subtilisin BPN'
    FEBS Open Bio (2022) 12, 2057-2064. PubMed  Europe PubMed DOI  PMC  EPMC  I
    3. 2021
  3. Chen,Y., Toth,E.A., Ruan,B., Choi,E.J., Simmerman,R., Chen,Y., He,Y., Wang,R., Godoy-Ruiz,R., King,H., Custer,G., Travis Gallagher,D., Rozak,D.A., Solomon,M., Muro,S., Weber,D.J., Orban,J., Fuerst,T.R. and Bryan,P.N.
    Engineering subtilisin proteases that specifically degrade active RAS
    Commun Biol (2021) 4, 299-299. PubMed  Europe PubMed DOI  PMC  EPMC
  4. Weeks,A.M., Byrnes,J.R., Lui,I. and Wells,J.A.
    Mapping proteolytic neo-N termini at the surface of living cells
    Proc Natl Acad Sci U S A (2021) 118 PubMed  Europe PubMed DOI
    5. 2020
  5. Che,Z., Cao,X., Chen,G. and Liang,Z.
    An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
    BMC Biotechnol (2020) 20, 63-63. PubMed  Europe PubMed DOI  PMC  EPMC
    6. 2015
  6. Iqbal,I., Aftab,M.N., Afzal,M., Ur-Rehman,A., Aftab,S., Zafar,A., Ud-Din,Z., Khuharo,A.R., Iqbal,J. and Ul-Haq,I.
    Purification and characterization of cloned alkaline protease gene of Geobacillus stearothermophilus
    J Basic Microbiol (2015) 55, 160-171. PubMed  Europe PubMed DOI  E
    7. 2012
  7. Graycar,T.P., Bott,R.R., Power,S.D. and Estell,D.A.
    Subtilisins
    [ISSN:978-0-12-407742-3] (2012) 3, 3148-3155. DOI
    8. 2011
  8. Jo,H.D., Lee,H.A., Jeong,S.J. and Kim,J.H.
    Purification and characterization of a major fibrinolytic enzyme from Bacillus amyloliquefaciens MJ5-41 isolated from meju
    J Microbiol Biotechnol (2011) 21, 1166-1173. PubMed  Europe PubMed
  9. Niu,Q., Tian,Y., Zhang,L., Xu,X., Niu,X., Xia,Z., Lei,L., Zhang,K.Q. and Huang,X.
    Overexpression of the key virulence proteases Bace16 and Bae16 in Bacillus nematocida B16 to improve its nematocidal activity
    J Mol Microbiol Biotechnol (2011) 21, 130-137. PubMed  Europe PubMed DOI
    10. 2010
  10. Huang,W., Tian,B.Y., Guo,J., Cai,W.L. and Huang,J.Z.
    Production of an in vitro-derived deletion mutation of Brevibacillus laterosporus by constructing a homology-driven integration vector
    Curr Microbiol (2010) 61, 401-406. PubMed  Europe PubMed DOI
    11. 2009
  11. Gallagher,T., Ruan,B., London,M., Bryan,M.A. and Bryan,P.N.
    Structure of a switchable subtilisin complexed with a substrate and with the activator azide
    Biochemistry (2009) 48, 10389-10394. PubMed  Europe PubMed DOI  S
  12. Tian,B., Huang,W., Huang,J., Jiang,X. and Qin,L.
    Investigation of protease-mediated cuticle-degradation of nematodes by using an improved immunofluorescence-localization method
    J Invertebr Pathol (2009) 101, 143-146. PubMed  Europe PubMed DOI
    13. 2007
  13. Knight,Z.A., Garrison,J.L., Chan,K., King,D.S. and Shokat,K.M.
    A remodelled protease that cleaves phosphotyrosine substrates
    J Am Chem Soc (2007) 129, 11672-11673. PubMed  Europe PubMed DOI
  14. Lian,L.H., Tian,B.Y., Xiong,R., Zhu,M.Z., Xu,J. and Zhang,K.Q.
    Proteases from Bacillus: a new insight into the mechanism of action for rhizobacterial suppression of nematode populations
    Lett Appl Microbiol (2007) 45, 262-269. PubMed  Europe PubMed DOI
  15. Niu,Q., Huang,X., Zhang,L., Lian,L., Li,Y., Li,J., Yang,J. and Zhang,K.
    Functional identification of the gene bace16 from nematophagous bacterium Bacillus nematocida
    Appl Microbiol Biotechnol (2007) 75, 141-148. PubMed  Europe PubMed DOI
  16. Tian,B., Yang,J., Lian,L., Wang,C., Li,N. and Zhang,K.Q.
    Role of an extracellular neutral protease in infection against nematodes by Brevibacillus laterosporus strain G4
    Appl Microbiol Biotechnol (2007) 74, 372-380. PubMed  Europe PubMed DOI
    17. 2006
  17. Taniguchi,M., Atiwetin,P., Hirai,T., Itoh,M., Harada,S., Hara,S. and Kamei,K.
    Interaction of subtilisin BPN' and recombinant fungal protease inhibitor F from silkworm with substituted P1 site residues
    Biosci Biotechnol Biochem (2006) 70, 1262-1264. PubMed  Europe PubMed DOI
  18. [YEAR:3-8-2006]Tian,B., Li,N., Lian,L., Liu,J., Yang,J. and Zhang,K.Q.
    Cloning, expression and deletion of the cuticle-degrading protease BLG4 from nematophagous bacterium Brevibacillus laterosporus G4
    Arch Microbiol (3-8-2006) 186, 297-305. PubMed  Europe PubMed DOI
    19. 2005
  19. Huang,X., Tian,B., Niu,Q., Yang,J., Zhang,L. and Zhang,K.
    An extracellular protease from Brevibacillus laterosporus G4 without parasporal crystals can serve as a pathogenic factor in infection of nematodes
    Res Microbiol (2005) 156, 719-727. PubMed  Europe PubMed DOI
    20. 2004
  20. [YEAR:15-7-2004]Choi,N.S., Chang,K.T., Jae Maeng,P. and Kim,S.H.
    Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4
    FEMS Microbiol Lett (15-7-2004) 236, 325-331. PubMed  Europe PubMed DOI
  21. Ganz,P.J., Bauer,M.D., Sun,Y., Fieno,A.M., Grant,R.A., Correa,P.E., Laskowski,M., Jr. and Saunders,C.W.
    Stabilized variant of Streptomyces subtilisin inhibitor and its use in stabilizing subtilisin BPN'
    Protein Eng Des Sel (2004) 17, 333-339. PubMed  Europe PubMed DOI
  22. Graycar,T.P., Ballinger,M.D. and Wells,J.A.
    Subtilisins
    [ISSN:0-12-079610-4] (2004) 2, 1786-1792.  V
    23. 2003
  23. Day,R.M., Thalhauser,C.J., Sudmeier,J.L., Vincent,M.P., Torchilin,E.V., Sanford,D.G., Bachovchin,C.W. and Bachovchin,W.W.
    Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN' by NMR
    Protein Sci (2003) 12, 794-810. PubMed  Europe PubMed DOI  PMC  EPMC
    24. 2002
  24. Almog,O., Gallagher,D.T., Ladner,J.E., Strausberg,S., Alexander,P., Bryan,P. and Gilliland,G.L.
    Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'
    J Biol Chem (2002) 277, 27553-27558. PubMed  Europe PubMed DOI  S
    25. 2001
  25. Alexander,P.A., Ruan,B. and Bryan,P.N.
    Cation-dependent stability of subtilisin
    Biochemistry (2001) 40, 10634-10639. PubMed  Europe PubMed DOI
  26. Kojima,S., Yanai,H. and Miura,K.
    Accelerated refolding of subtilisin BPN' by tertiary-structure-forming mutants of its propeptide
    J Biochem (2001) 130, 471-474. PubMed  Europe PubMed
    27. 2000
  27. Yokota,M., Tsuda,I. and Tatsumi,N.
    Availability of Nagarse for DNA analysis as a substitute for proteinase K
    J Clin Lab Anal (2000) 14, 97-100. PubMed  Europe PubMed DOI
    28. 1999
  28. Dinakarpandian,D., Shenoy,B.C., Hilvert,D., McRee,D.E., McTigue,M. and Carey,P.R.
    Electric fields in active sites: substrate switching from null to strong fields in thiol- and selenol-subtilisins
    Biochemistry (1999) 38, 6659-6667. PubMed  Europe PubMed DOI
    29. 1998
  29. Almog,O., Gallagher,T., Tordova,M., Hoskins,J., Bryan,P. and Gilliland,G.L.
    Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain
    Proteins (1998) 31, 21-32. PubMed  Europe PubMed DOI  S
    30. 1997
  30. Huang,H.W., Chen,W.C., Wu,C.Y., Yu,H.C., Lin,W.Y., Chen,S.T. and Wang,K.T.
    Kinetic studies of the inhibitory effects of propeptides subtilisin BPN' and Carlsberg to bacterial serine proteases
    Protein Eng (1997) 10, 1227-1233. PubMed  Europe PubMed
    31. 1996
  31. Ballinger,M.D., Tom,J. and Wells,J.A.
    Furilisin: a variant of subtilisin BPN' engineered for cleaving tribasic substrates
    Biochemistry (1996) 35, 13579-13585. PubMed  Europe PubMed DOI
  32. Gallagher,T., Oliver,J., Bott,R., Betzel,C. and Gilliland,G.L.
    Subtilisin BPN' at 1.6 A resolution: analysis for discrete disorder and comparison of crystal forms
    Acta Crystallogr D Biol Crystallogr (1996) 52, 1125-1135. PubMed  Europe PubMed DOI  S
    33. 1995
  33. [YEAR:17-10-1995]Ballinger,M.D., Tom,J. and Wells,J.A.
    Designing subtilisin BPN' to cleave substrates containing dibasic residues
    Biochemistry (17-10-1995) 34, 13312-13319. PubMed  Europe PubMed DOI
  34. Gallagher,T., Gilliland,G., Wang,L. and Bryan,P.
    The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'
    Structure (1995) 3, 907-914. PubMed  Europe PubMed
  35. Genov,N., Filippi,B., Dolashka,P., Wilson,K.S. and Betzel,C.
    Stability of subtilisins and related proteinases (subtilases)
    Int J Pept Protein Res (1995) 45, 391-400. PubMed  Europe PubMed
  36. Strausberg,S.L., Alexander,P.A., Gallagher,D.T., Gilliland,G.L., Barnett,B.L. and Bryan,P.N.
    Directed evolution of a subtilisin with calcium-independent stability
    Biotechnology (N Y) (1995) 13, 669-673. PubMed  Europe PubMed
    37. 1994
  37. [YEAR:11-1-1994]Rheinnecker,M., Eder,J., Pandey,P.S. and Fersht,A.R.
    Variants of subtilisin BPN' with altered specificity profiles
    Biochemistry (11-1-1994) 33, 221-225. PubMed  Europe PubMed
    38. 1993
  38. Eder,J., Rheinnecker,M. and Fersht,A.R.
    Folding of subtilisin BPN': role of the pro-sequence
    J Mol Biol (1993) 233, 293-304. PubMed  Europe PubMed DOI
  39. [YEAR:13-12-1993]Eder,J., Rheinnecker,M. and Fersht,A.R.
    Hydrolysis of small peptide substrates parallels binding of chymotrypsin inhibitor 2 for mutants of subtilisin BPN'
    FEBS Lett (13-12-1993) 335, 349-352. PubMed  Europe PubMed DOI
  40. [YEAR:21-12-1993]Jackson,S.E. and Fersht,A.R.
    Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'
    Biochemistry (21-12-1993) 32, 13909-13916. PubMed  Europe PubMed  I
  41. Stoffer,B., Frandsen,T.P., Busk,P.K., Schneider,P., Svendsen,I. and Svensson,B.
    Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger
    Biochem J (1993) 292, 197-202. PubMed  Europe PubMed  PMC  EPMC  P
    42. 1992
  42. [YEAR:1-9-1992]Braxton,S. and Wells,J.A.
    Incorporation of a stabilizing Ca(2+)-binding loop into subtilisin BPN'
    Biochemistry (1-9-1992) 31, 7796-7801. PubMed  Europe PubMed
  43. Bryan,P., Alexander,P., Strausberg,S., Schwarz,F., Lan,W., Gilliland,G. and Gallagher,D.T.
    Energetics of folding subtilisin BPN'
    Biochemistry (1992) 31, 4937-4945. PubMed  Europe PubMed DOI
  44. Gron,H., Meldal,M. and Breddam,K.
    Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching
    Biochemistry (1992) 31, 6011-6018. PubMed  Europe PubMed DOI
    45. 1991
  45. [YEAR:25-6-1991]Braxton,S. and Wells,J.A.
    The importance of a distal hydrogen bonding group in stabilizing the transition state in subtilisin BPN'
    J Biol Chem (25-6-1991) 266, 11797-11800. PubMed  Europe PubMed
  46. [YEAR:25-6-1991]Carter,P., Abrahmsen,L. and Wells,J.A.
    Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'
    Biochemistry (25-6-1991) 30, 6142-6148. PubMed  Europe PubMed
  47. Siezen,R.J., de Vos,W.M., Leunissen,J.A. and Dijkstra,B.W.
    Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases
    Protein Eng (1991) 4, 719-737. PubMed  Europe PubMed
  48. Takeuchi,Y., Satow,Y., Nakamura,K.T. and Mitsui,Y.
    Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8 A resolution
    J Mol Biol (1991) 221, 309-325. PubMed  Europe PubMed
  49. Takeuchi,Y., Noguchi,S., Satow,Y., Kojima,S., Kumagai,I., Miura,K., Nakamura,K.T. and Mitsui,Y.
    Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor)
    Protein Eng (1991) 4, 501-508. PubMed  Europe PubMed  S
    50. 1990
  50. Carter,P. and Wells,J.A.
    Functional interaction among catalytic residues in subtilisin BPN'
    Proteins (1990) 7, 335-342. PubMed  Europe PubMed DOI
  51. Erwin,C.R., Barnett,B.L., Oliver,J.D. and Sullivan,J.F.
    Effects of engineered salt bridges on the stability of subtilisin BPN'
    Protein Eng (1990) 4, 87-97. PubMed  Europe PubMed  S
    52. 1989
  52. Carter,P., Nilsson,B., Burnier,J.P., Burdick,D. and Wells,J.A.
    Engineering subtilisin BPN' for site-specific proteolysis
    Proteins (1989) 6, 240-248. PubMed  Europe PubMed DOI
  53. [YEAR:5-9-1989]Pantoliano,M.W., Whitlow,M., Wood,J.F., Dodd,S.W., Hardman,K.D., Rollence,M.L. and Bryan,P.N.
    Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding
    Biochemistry (5-9-1989) 28, 7205-7213. PubMed  Europe PubMed
    54. 1988
  54. [YEAR:5-6-1988]Bott,R., Ultsch,M., Kossiakoff,A., Graycar,T., Katz,B. and Power,S.
    The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation
    J Biol Chem (5-6-1988) 263, 7895-7906. PubMed  Europe PubMed  S
  55. McPhalen,C.A. and James,M.N.
    Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo
    Biochemistry (1988) 27, 6582-6598. PubMed  Europe PubMed  S
    56. 1987
  56. [YEAR:24-7-1987]Carter,P. and Wells,J.A.
    Engineering enzyme specificity by "substrate-assisted catalysis"
    Science (24-7-1987) 237, 394-399. PubMed  Europe PubMed
    57. 1986
  57. [YEAR:1-2-1986]Bromme,D., Peters,K., Fink,S. and Fittkau,S.
    Enzyme-substrate interactions in the hydrolysis of peptide substrates by thermitase, subtilisin BPN', and proteinase K
    Arch Biochem Biophys (1-2-1986) 244, 439-446. PubMed  Europe PubMed
    58. 1985
  58. Mitsui,Y., Akagawa,H., Onishi,H., Itoh,S., Nakamura,K.T. and Iitaka,Y.
    Crystal structure of subtilisin complexed with its trapped substrate Streptomyces subtilisin inhibitor - protein-protein interaction and evolution of serine proteinases and their proteinaceous inhibitors
    J Biosci (1985) 8, 481-489. DOI  S
  59. Nedkov,P., Oberthur,W. and Braunitzer,G.
    Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN', Carlsberg and amylosacchariticus
    Biol Chem Hoppe Seyler (1985) 366, 421-430. PubMed  Europe PubMed
    60. 1984
  60. [YEAR:15-9-1984]Hirono,S., Akagawa,H., Mitsui,Y. and Iitaka,Y.
    Crystal structure at 2.6 A resolution of the complex of subtilisin BPN' with streptomyces subtilisin inhibitor
    J Mol Biol (15-9-1984) 178, 389-414. PubMed  Europe PubMed  S
    61. 1983
  61. Wells,J.A., Ferrari,E., Henner,D.J., Estell,D.A. and Chen,E.Y.
    Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis
    Nucleic Acids Res (1983) 11, 7911-7925. PubMed  Europe PubMed  PMC  EPMC
    62. 1977
  62. Priest,F.G.
    Extracellular enzyme synthesis in the genus Bacillus
    Bacteriol Rev (1977) 41, 711-753. PubMed  Europe PubMed  PMC  EPMC
    63. 1975
  63. [YEAR:25-9-1975]Matthews,D.A., Alden,R.A., Birktoft,J.J., Freer,S.T. and Kraut,J.
    X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo). A model for the catalytic transition state
    J Biol Chem (25-9-1975) 250, 7120-7126. PubMed  Europe PubMed  S  I
    64. 1972
  64. Adams,K.R.
    Investigations on the chemical reactivity of the carboxyl groups in subtilisin type Novo
    C R Trav Lab Carlsberg [Chim] (1972) 38, 481-498.
  65. [YEAR:27-3-1972]Drenth,J., Hol,W.G., Jansonius,J.N. and Koekoek,R.
    Subtilisin Novo. The three-dimensional structure and its comparison with subtilisin BPN'
    Eur J Biochem (27-3-1972) 26, 177-181. PubMed  Europe PubMed  S
  66. [YEAR:20-6-1972]Robertus,J.D., Alden,R.A., Birktoft,J.J., Kraut,J., Powers,J.C. and Wilcox,P.E.
    An X-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN'
    Biochemistry (20-6-1972) 11, 2439-2449. PubMed  Europe PubMed  S  I
    67. 1971
  67. Alden,R.A., Birktoft,J.J., Kraut,J., Robertus,J.D. and Wright,C.S.
    Atomic coordinates for subtilisin BPN' (or Novo)
    Biochem Biophys Res Commun (1971) 45, 337-344. PubMed  Europe PubMed  S
  68. [YEAR:22-1-1971]Robertus,J.D., Alden,R.A. and Kraut,J.
    On the identity of subtilisins BPN' and Novo
    Biochem Biophys Res Commun (22-1-1971) 42, 334-339. PubMed  Europe PubMed
    69. 1970
  69. Morihara,K. and Oka,T.
    Subtilisin BPN': inactivation by chloromethyl ketone derivates of peptide substrates
    Arch Biochem Biophys (1970) 138, 526-531. PubMed  Europe PubMed
  70. Ottesen,M. and Svendsen,A.
    [11] The subtilisins
    Methods Enzymol (1970) 19, 199-215. DOI  V
    71. 1969
  71. [YEAR:18-1-1969]Wright,C.S., Alden,R.A. and Kraut,J.
    Structure of subtilisin BPN' at 2.5 angstrom resolution
    Nature (18-1-1969) 221, 235-242. PubMed  Europe PubMed DOI  S
    72. 1966
  72. Smith,E.L., Markland,F.S., Kasper,C.B., DeLange,R.J., Landon,M. and Evans,W.H.
    The complete amino acid sequence of two types of subtilisin, BPN' and Carlsberg
    J Biol Chem (1966) 241, 5974-5976. PubMed  Europe PubMed
    73. 1960
  73. Sanger,F. and Shaw,D.C.
    Amino-acid sequence about the reactive serine of a proteolytic enzyme from Bacillus subtilis
    Nature (1960) 187, 872-873. PubMed  Europe PubMed DOI  I
    74. 1956
  74. Okunuki,K., Matsubara,H., Nishimura,S. and Hagihara,B.
    Specificity of crystalline bacterial proteinase
    J Biochem (1956) 43, 857-866.  P

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