Substrates for peptidase M13.014: neprilysin-4 (Drosophila melanogaster)

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
adipokinetic hormone	P61855	23-30	Gln-Leu+Thr-Phe-Ser-Pro-Asp-Trp	P	MS	'-'	'-'	Gln	Leu	Thr	Phe	Ser	Pro	Hallier et al., 2016
adipokinetic hormone	P61855	23-30	Gln-Leu-Thr+Phe-Ser-Pro-Asp-Trp	P	MS	'-'	Gln	Leu	Thr	Phe	Ser	Pro	Asp	Hallier et al., 2016
allatostatin-A	Q9VC44	57-64	Val-Glu-Arg-Tyr-Ala-Phe-Gly+Leu	P	MS	Tyr	Ala	Phe	Gly	Leu	'-'	'-'	'-'	Hallier et al., 2016
allatostatin-A	Q9VC44	57-64	Val-Glu-Arg-Tyr-Ala-Phe+Gly-Leu	P	MS	Arg	Tyr	Ala	Phe	Gly	Leu	'-'	'-'	Hallier et al., 2016
allatostatin-A	Q9VC44	81-88	Leu-Pro-Val-Tyr-Asn-Phe-Gly+Leu	P	MS	Tyr	Asn	Phe	Gly	Leu	'-'	'-'	'-'	Hallier et al., 2016
allatostatin-A	Q9VC44	81-88	Leu-Pro-Val-Tyr-Asn-Phe+Gly-Leu	P	MS	Val	Tyr	Asn	Phe	Gly	Leu	'-'	'-'	Hallier et al., 2016
allatostatin-A	Q9VC44	92-99	Ser-Arg-Pro+Tyr-Ser-Phe-Gly-Leu	P	MS	'-'	Ser	Arg	Pro	Tyr	Ser	Phe	Gly	Hallier et al., 2016
allatostatin-A	Q9VC44	138-148	Thr-Thr-Arg-Pro-Gln-Pro-Phe-Asn-Phe-Gly+Leu	P	MS	Phe	Asn	Phe	Gly	Leu	'-'	'-'	'-'	Hallier et al., 2016
allatostatin-A	Q9VC44	138-148	Thr-Thr-Arg-Pro-Gln-Pro-Phe-Asn+Phe-Gly-Leu	P	MS	Gln	Pro	Phe	Asn	Phe	Gly	Leu	'-'	Hallier et al., 2016
allatostatin-A	Q9VC44	138-148	Thr-Thr-Arg-Pro-Gln-Pro+Phe-Asn-Phe-Gly-Leu	P	MS	Arg	Pro	Gln	Pro	Phe	Asn	Phe	Gly	Hallier et al., 2016
diuretic hormone class 2	Q9VLK4	76-106	peptide-Gly84+Tyr-peptide	P	MS	Leu	Ala	Arg	Gly	Tyr	Ser	Gly	Thr	Hallier et al., 2016
diuretic hormone class 2	Q9VLK4	76-106	peptide-Gly96+Leu-peptide	P	MS	His	Arg	Met	Gly	Leu	Ala	Ala	Ala	Hallier et al., 2016
drosulfakinins	P09040	114-122	Phe-Asp-Asp-Tyr-Gly-His-Met-Arg+Phe	P	MS	Gly	His	Met	Arg	Phe	'-'	'-'	'-'	Hallier et al., 2016
drosulfakinins	P09040	126-139	Gly-Gly-Asp-Asp-Gln-Phe-Asp-Asp-Tyr-Gly-His-Met-Arg+Phe	P	MS	Gly	His	Met	Arg	Phe	'-'	'-'	'-'	Hallier et al., 2016
drosulfakinins	P09040	126-139	Gly-Gly-Asp-Asp-Gln+Phe-Asp-Asp-Tyr-Gly-His-Met-Arg-Phe	P	MS	Gly	Asp	Asp	Gln	Phe	Asp	Asp	Tyr	Hallier et al., 2016
leucokinin	P81829	133-147	Asn-Ser-Val-Val-Leu-Gly-Lys-Lys-Gln-Arg+Phe-His-Ser-Trp-Gly	P	MS	Lys	Lys	Gln	Arg	Phe	His	Ser	Trp	Hallier et al., 2016
leucokinin	P81829	133-147	Asn-Ser-Val-Val-Leu-Gly-Lys-Lys-Gln-Arg-Phe-His+Ser-Trp-Gly	P	MS	Gln	Arg	Phe	His	Ser	Trp	Gly	'-'	Hallier et al., 2016
leucokinin	P81829	133-147	Asn-Ser-Val-Val-Leu-Gly-Lys-Lys-Gln-Arg-Phe-His-Ser+Trp-Gly	P	MS	Arg	Phe	His	Ser	Trp	Gly	'-'	'-'	Hallier et al., 2016
pro-corazonin	Q26377	20-30	Gln-Thr+Phe-Gln-Tyr-Ser-Arg-Gly-Trp-Thr-Asn	P	MS	'-'	'-'	Gln	Thr	Phe	Gln	Tyr	Ser	Hallier et al., 2016
pro-corazonin	Q26377	20-30	Gln-Thr-Phe-Gln-Tyr-Ser-Arg-Gly+Trp-Thr-Asn	P	MS	Tyr	Ser	Arg	Gly	Trp	Thr	Asn	'-'	Hallier et al., 2016
protachykinin-1	P20366	58-68	Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe+Gly-Leu-Met	N		Gln	Gln	Phe	Phe	Gly	Leu	Met	-	Meyer et al., 2009
protachykinin-1	P20366	58-68	Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly+Leu-Met	N		Gln	Phe	Phe	Gly	Leu	Met	-	-	Meyer et al., 2009
short neuropeptide F	Q9VIQ0	104-111	Ser-Pro-Ser-Leu-Arg-Leu-Arg+Phe	P	MS	Leu	Arg	Leu	Arg	Phe	'-'	'-'	'-'	Hallier et al., 2016
short neuropeptide F	Q9VIQ0	67-77	Ala-Gln-Arg-Ser-Pro-Ser-Leu-Arg-Leu+Arg-Phe	P	MS	Ser	Leu	Arg	Leu	Arg	Phe	'-'	'-'	Hallier et al., 2016
tachykinins	Q9VGE8	50-59	Ala-Pro-Thr-Ser-Ser+Phe-Ile-Gly-Met-Arg	P	MS	Pro	Thr	Ser	Ser	Phe	Ile	Gly	Met	Hallier et al., 2016
tachykinins	Q9VGE8	50-59	Ala-Pro-Thr-Ser-Ser-Phe-Ile-Gly+Met-Arg	P	MS	Ser	Phe	Ile	Gly	Met	Arg	'-'	'-'	Hallier et al., 2016
tachykinins	Q9VGE8	102-110	Ala-Pro+Leu-Ala-Phe-Val-Gly-Leu-Arg	P	MS	'-'	'-'	Ala	Pro	Leu	Ala	Phe	Val	Hallier et al., 2016
tachykinins	Q9VGE8	102-110	Ala-Pro-Leu-Ala+Phe-Val-Gly-Leu-Arg	P	MS	Ala	Pro	Leu	Ala	Phe	Val	Gly	Leu	Hallier et al., 2016
tachykinins	Q9VGE8	102-110	Ala-Pro-Leu-Ala-Phe+Val-Gly-Leu-Arg	P	MS	Pro	Leu	Ala	Phe	Val	Gly	Leu	Arg	Hallier et al., 2016
tachykinins	Q9VGE8	102-110	Ala-Pro-Leu-Ala-Phe-Val-Gly+Leu-Arg	P	MS	Ala	Phe	Val	Gly	Leu	Arg	'-'	'-'	Hallier et al., 2016
tachykinins	Q9VGE8	191-200	Ala-Pro-Val-Asn-Ser-Phe-Val-Gly+Met-Arg	P	MS	Ser	Phe	Val	Gly	Met	Arg	'-'	'-'	Hallier et al., 2016
tachykinins	Q9VGE8	273-281	Ala-Pro-Asn-Gly+Phe-Leu-Gly-Met-Arg	P	MS	Ala	Pro	Asn	Gly	Phe	Leu	Gly	Met	Hallier et al., 2016