Substrates for peptidase C65.001: otubain-1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
Ac-Leu-Arg-Gly-Gly-AFC			Ac-Leu-Arg-Gly-Gly+AFC	S		Leu	Arg	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Lys-Arg-Gly-Gly-AFC			Ac-Lys-Arg-Gly-Gly+AFC	S		Lys	Arg	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Nle-Arg-Gly-Gly-AFC			Ac-Nle-Arg-Gly-Gly+AFC	S		Nle	Arg	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Phe-Arg-Gly-Gly-AFC			Ac-Phe-Arg-Gly-Gly+AFC	S		Phe	Arg	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Trp-Arg-Gly-Gly-AFC			Ac-Trp-Arg-Gly-Gly+AFC	S		Trp	Arg	Gly	Gly	AFC	-	-	-	Drag et al., 2008
Ac-Tyr-Arg-Gly-Gly-AFC			Ac-Tyr-Arg-Gly-Gly+AFC	S		Tyr	Arg	Gly	Gly	AFC	-	-	-	Drag et al., 2008
diubiquitin	P0CG47	1-76	peptide-Gly76+Lys48-peptide	P		Leu	Arg	Gly	Gly	Lys	Gln	Leu	Glu	Mevissen et al., 2013
diubiquitin	P0CG47	1-76	peptide-Gly76+Lys48-peptide	P		Leu	Arg	Gly	Gly	Lys	Gln	Leu	Glu	Mevissen et al., 2013
Lys48-linked tetra-ubiquitin	P0CG47	1-76	Peptide-Gly76+Lys48-Peptide	P	MS	Leu	Arg	Gly	Gly	Lys	Gln	Leu	Glu	Edelmann et al., 2009
Lys63-linked tetra-ubiquitin	P0CG47	1-76	Peptide-Gly76+Lys63-Peptide	P	MS	Leu	Arg	Gly	Gly	Lys	Glu	Ser	Thr	Edelmann et al., 2009
NEDD8-(Biotin)-Val-Lys-Ala-Lys-Ile-Gln-Asp conjugate			Peptide-Gly76+Lys-Ile-Gln-Asp	S	AB	Leu	Arg	Gly	Gly	Lys	Ile	Gln	Asp	Edelmann et al., 2009
polyubiquitinated estrogen receptor	P0CG47/P03372	1-76	peptide-Gly76+Lys-peptide	P		Leu	Arg	Gly	Gly	Lys	Xaa	Xaa	Xaa	Stanisic et al., 2009
polyubiquitinated TNF receptor-associated factor 3	P0CG47/Q13114	1-76	Peptide-Gly76+Lys63-Peptide	P		Leu	Arg	Gly	Gly	Lys	Glu	Ser	Thr	Li et al., 2010
Ubiquitin-(Biotin)-Val-Lys-Ala-Lys-Ile-Gln-Asp conjugate			Peptide-Gly76+Lys-Ile-Gln-Asp	S	AB	Leu	Arg	Gly	Gly	Lys	Ile	Gln	Asp	Edelmann et al., 2009