Substrates for peptidase C19.010: ubiquitin-specific peptidase 4

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
polyubiquitin	P0CG47/P0CG47	1-76	peptide-Gly76+Met1-peptide	N	Leu	Arg	Gly	Gly	Met	Gln	Ile	Phe	Baker, 2004
polyubiquitinated adenosine receptor A2a	P0CG47/P29274	1-76	peptide-Gly76+peptide	P	Leu	Arg	Gly	Gly	Xaa	Xaa	Xaa	Xaa	Milojevic et al., 2006
polyubiquitinated E3 ubiquitin-protein ligase TRIM21	P0CG47/P19474	1-76	peptide-Gly76+Lys-peptide	P	Leu	Arg	Gly	Gly	Xaa	Xaa	Xaa	Xaa	Wada et al., 2006
Ub-(epsilonN)-Lys	P62988	1-76	peptide-Gly76+(epsilonN)-Lys	S	Leu	Arg	Gly	Gly	Lys	-	-	-	Layfield et al., 1999
UBICEP52	P0CG47	1-76	peptide-Gly76+Ile1-peptide	N	Leu	Arg	Gly	Gly	Ile	Ile	Glu	Pro	Layfield et al., 1999
ubiquitin-beta-galactosidase fusion protein	P0CG47	1-76	peptide-Gly76+Arg1-peptide	N	Leu	Arg	Gly	Gly	Arg	His	Gly	Ser	Baker, 2004
ubiquitin-glutathione S-transferase P fusion protein	P0CG47	1-76	peptide-Gly76+Pro-peptide	N	Leu	Arg	Gly	Gly	Pro	-	-	-	Baker, 2004
ubiquitinated 3-phosphoinositide-dependent protein kinase 1	P0CG47/O15530	1-76	peptide-Gly76+Lys-peptide	P	Leu	Arg	Gly	Gly	Xaa	Xaa	Xaa	Xaa	Uras et al., 2012
ubiquitinated glutathione S-transferase P	P0CG47	1-76	peptide-Gly76+Met-peptide	N	Leu	Arg	Gly	Gly	Met	Pro	Pro	Tyr	Baker, 2004
ubiquitinated UbCEP52	P0CG47	1-76	peptide-Gly76+Ile1-peptide	N	Leu	Arg	Gly	Gly	Ile	Ile	Glu	Pro	Baker, 2004
ubiquitinated Ubi2p ({Saccharomyces cerevisiae})		1-76	peptide-Gly76+Ile-peptide	N	Leu	Arg	Gly	Gly	Ile	Ile	Glu	Pro	Baker, 2004
ubiquitinated Ubi3p ({Saccharomyces cerevisiae})		1-76	peptide-Gly76+Gly-peptide	N	Leu	Arg	Gly	Gly	Gly	Lys	Lys	Arg	Baker, 2004