Substrates for peptidase C01.144: cathepsin B-like cysteine peptidase (Raphanus sativus)

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
Abz-Phe-Arg-NPh			Abz-Phe-Arg+NPh	S	-	Abz	Phe	Arg	NPh	-	-	-	Tsuji et al., 2008
beta-casein	P02666	16-224	peptide-Gln69+Thr-peptide	N	Pro	Phe	Ala	Gln	Thr	Gln	Ser	Leu	Tsuji et al., 2008
beta-casein	P02666	16-224	peptide-Ser72+Leu-peptide	N	Gln	Thr	Gln	Ser	Leu	Val	Tyr	Pro	Tsuji et al., 2008
beta-casein	P02666	16-224	peptide-Gly109+Val-peptide	N	Glu	Val	Met	Gly	Val	Ser	Lys	Val	Tsuji et al., 2008
beta-casein	P02666	16-224	peptide-Lys114+Glu-peptide	N	Ser	Lys	Val	Lys	Glu	Ala	Met	Ala	Tsuji et al., 2008
Boc-Gln-Arg-Arg-NHMec			Boc-Gln-Arg-Arg+NHMec	S	Boc	Gln	Arg	Arg	AMC	-	-	-	Tsuji et al., 2008
Boc-Leu-Arg-Arg-NHMec			Boc-Leu-Arg-Arg+NHMec	S	Boc	Leu	Arg	Arg	AMC	-	-	-	Tsuji et al., 2008
Corticotropin	P01189	138-176	peptide-His143+Phe-peptide	N	Ser	Met	Glu	His	Phe	Arg	Trp	Gly	Tsuji et al., 2008
Corticotropin	P01189	138-176	peptide-Phe144+Arg-peptide	N	Met	Glu	His	Phe	Arg	Trp	Gly	Lys	Tsuji et al., 2008
Corticotropin	P01189	138-176	peptide-Arg145+Trp-peptide	N	Glu	His	Phe	Arg	Trp	Gly	Lys	Pro	Tsuji et al., 2008
Corticotropin	P01189	138-176	peptide-Gly151+Lys-peptide	N	Lys	Pro	Val	Gly	Lys	Lys	Arg	Arg	Tsuji et al., 2008
Corticotropin	P01189	138-176	peptide-Val157+Lys-peptide	N	Arg	Arg	Pro	Val	Lys	Val	Tyr	Pro	Tsuji et al., 2008
Corticotropin	P01189	138-176	peptide-Lys158+Val-peptide	N	Arg	Pro	Val	Lys	Val	Tyr	Pro	Asn	Tsuji et al., 2008
Corticotropin	P01189	138-176	peptide-Val159+Tyr-peptide	N	Pro	Val	Lys	Val	Tyr	Pro	Asn	Gly	Tsuji et al., 2008
glucagon	P01275	53-81	peptide-Gln76+Trp-peptide	N	Asp	Phe	Val	Gln	Trp	Leu	Met	Asn	Tsuji et al., 2008
glucagon	P01275	53-81	peptide-Met79+Asn-Thr	N	Gln	Trp	Leu	Met	Asn	Thr	-	-	Tsuji et al., 2008
glucagon	P01275	53-81	peptide-Asp73+Phe-peptide	N	Arg	Ala	Gln	Asp	Phe	Val	Gln	Trp	Tsuji et al., 2008
glucagon	P01275	53-81	peptide-Val75+Gln-peptide	N	Gln	Asp	Phe	Val	Gln	Trp	Leu	Met	Tsuji et al., 2008
glucagon	P01275	53-81	peptide-Lys64+Tyr-peptide	N	Asp	Tyr	Ser	Lys	Tyr	Leu	Asp	Ser	Tsuji et al., 2008
glucagon	P01275	53-81	peptide-Leu66+Asp-peptide	N	Ser	Lys	Tyr	Leu	Asp	Ser	Arg	Arg	Tsuji et al., 2008
glucagon	P01275	53-81	peptide-Arg70+Ala-peptide	N	Asp	Ser	Arg	Arg	Ala	Gln	Asp	Phe	Tsuji et al., 2008
Pyr-Arg-Thr-Lys-Arg-NHMec			Pyr-Arg-Thr-Lys-Arg+NHMec	S	Arg	Thr	Lys	Arg	AMC	-	-	-	Tsuji et al., 2008
Z-Arg-Arg-NHMec			Z-Arg-Arg-NHMec	S	-	Z	Arg	Arg	AMC	-	-	-	Tsuji et al., 2008
Z-Phe-Arg-NHMec			Z-Phe-Arg-NHMec	S	-	Z	Phe	Arg	AMC	-	-	-	Tsuji et al., 2008