Structure for family M27 unassigned peptidases

Summary Sequences Sequence features Distribution Structure Literature Substrates Pharma

TERTIARY STRUCTURE DATA
Comment	Resolution	PDB	PDBe	SCOP	CATH	PDBSum	Proteopedia	Reference
Clostridium botulinum
light chain only	1.80 Å	1E1H	1E1H	1E1H	1E1H	1E1H	1E1H	Segelke et al., 2004
mature peptidase	1.80 Å	1E1H	1E1H	1E1H	1E1H	1E1H	1E1H	Segelke et al., 2004
catalytic domain	2.16 Å	1T3A	1T3A	1T3A	1T3A	1T3A	1T3A	Agarwal et al., 2004
clostridium botulinum type e catalytic domain e212q mutant	1.90 Å	1T3C	1T3C	1T3C	1T3C	1T3C	1T3C	Agarwal et al., 2004
mature peptidase	0.00 Å	1UEE	1UEE	1UEE	1UEE	1UEE	1UEE
Glu224Gln, Tyr366Phe double mutant	2.20 Å	1XTF	1XTF	1XTF	1XTF	1XTF	1XTF	Breidenbach & Brunger, 2004
complex with synaptosomal-associated protein 25	2.10 Å	1XTG	1XTG	1XTG	1XTG	1XTG	1XTG	Breidenbach & Brunger, 2004
Arg347Ala mutant; catalytic domain	2.17 Å	1ZKW	1ZKW	1ZKW	1ZKW	1ZKW	1ZKW	Agarwal et al., 2005
Glu158Ala/Thr159Ala/Asn160Ala triple mutant; catalytic domain	2.52 Å	1ZKX	1ZKX	1ZKX	1ZKX	1ZKX	1ZKX	Agarwal et al., 2005
Glu335Gln mutant; catalytic domain	2.60 Å	1ZL5	1ZL5	1ZL5	1ZL5	1ZL5	1ZL5	Agarwal et al., 2005
Tyr350Ala mutant; catalytic domain	2.40 Å	1ZL6	1ZL6	1ZL6	1ZL6	1ZL6	1ZL6	Agarwal et al., 2005
Glu335Ala mutant	2.60 Å	1ZN3	1ZN3	1ZN3	1ZN3	1ZN3	1ZN3	Agarwal et al., 2005
light chain	2.00 Å	2A8A	2A8A	2A8A	2A8A	2A8A	2A8A	Agarwal et al., 2005
catalytic domain	1.80 Å	2A97	2A97	2A97	2A97	2A97	2A97	Agarwal et al., 2005
light chain only	1.65 Å	2FPQ	2FPQ	2FPQ	2FPQ	2FPQ	2FPQ	Arndt et al., 2006
structure of the light chain of botulinum neurotoxin, serotype a bound to small molecule inhibitors	2.80 Å	2G7K	2G7K	2G7K	2G7K	2G7K	2G7K	Fu et al., 2006
structure of the light chain of botulinum neurotoxin serotype a bound to small molecule inhibitors	1.90 Å	2G7N	2G7N	2G7N	2G7N	2G7N	2G7N
structure of the light chain of botulinum neurotoxin serotype a bound to small molecule inhibitors	2.30 Å	2G7P	2G7P	2G7P	2G7P	2G7P	2G7P	Fu et al., 2006
structure of the light chain of botulinum neurotoxin serotype a bound to small molecule inhibitors	2.41 Å	2G7Q	2G7Q	2G7Q	2G7Q	2G7Q	2G7Q	Fu et al., 2006
clostridium botulinum serotype a light chain inhibited by 4- chlorocinnamic hydroxamate	1.90 Å	2ILP	2ILP	2ILP	2ILP	2ILP	2ILP	Silvaggi et al., 2007
clostridium botulinum neurotoxin serotype a light chain inhibited by 2,4-dichlorocinnamic hydroxamate	1.94 Å	2IMA	2IMA	2IMA	2IMA	2IMA	2IMA	Silvaggi et al., 2007
clostridium botulinum neurotoxin serotype a light chain inhibited by l-arginine hydroxamate	2.41 Å	2IMB	2IMB	2IMB	2IMB	2IMB	2IMB	Silvaggi et al., 2007
light chain only	2.00 Å	2IMC	2IMC	2IMC	2IMC	2IMC	2IMC	Silvaggi et al., 2007
light chain; crystal form A	2.20 Å	2ISE	2ISE	2ISE	2ISE	2ISE	2ISE	Burnett et al., 2007
light chain; crystal form B	2.00 Å	2ISG	2ISG	2ISG	2ISG	2ISG	2ISG	Burnett et al., 2007
light chain; crystal form c	2.00 Å	2ISH	2ISH	2ISH	2ISH	2ISH	2ISH	Burnett et al., 2007
complex with monoclonal antibody CR1	2.61 Å	2NYY	2NYY	2NYY	2NYY	2NYY	2NYY	Garcia-Rodriguez et al., 2007
complex with monoclonal antibody AR2	3.79 Å	2NZ9	2NZ9	2NZ9	2NZ9	2NZ9	2NZ9	Garcia-Rodriguez et al., 2007
structure of botulinum neurotoxin serotype c1 light chain protease	1.75 Å	2QN0	2QN0	2QN0	2QN0	2QN0	2QN0	Jin et al., 2007
mature peptidase	1.60 Å	2VU9	2VU9	2VU9	2VU9	2VU9	2VU9	Stenmark et al., 2008
mature peptidase	1.70 Å	2VUA	2VUA	2VUA	2VUA	2VUA	2VUA	Stenmark et al., 2008
mature peptidase	2.59 Å	2W2D	2W2D	2W2D	2W2D	2W2D	2W2D	Masuyer et al., 2009
apo-enzyme	1.25 Å	3BOK	3BOK	3BOK	3BOK	3BOK	3BOK	Silvaggi et al., 2008
with zinc bound	1.20 Å	3BON	3BON	3BON	3BON	3BON	3BON	Silvaggi et al., 2008
complex with inhibitory peptide	1.40 Å	3BOO	3BOO	3BOO	3BOO	3BOO	3BOO	Silvaggi et al., 2008
mature peptidase	3.20 Å	3BTA	3BTA	3BTA	3BTA	3BTA	3BTA	Lacy et al., 1998
mature peptidase	1.70 Å	3BWI	3BWI	3BWI	3BWI	3BWI	3BWI	Kumaran et al., 2008
catalytic domain from serotype A; complex with inhibitory peptide RRGC	1.60 Å	3C88	3C88	3C88	3C88	3C88	3C88
catalytic domain from serotype A; complex with inhibitory peptide RRGM	1.58 Å	3C89	3C89	3C89	3C89	3C89	3C89
mature peptidase	1.52 Å	3C8A	3C8A	3C8A	3C8A	3C8A	3C8A	Kumaran et al., 2008
mature peptidase	1.47 Å	3C8B	3C8B	3C8B	3C8B	3C8B	3C8B	Kumaran et al., 2008
mature peptidase	1.50 Å	3DDA	3DDA	3DDA	3DDA	3DDA	3DDA	Kumaran et al., 2008
mature peptidase	1.60 Å	3DDB	3DDB	3DDB	3DDB	3DDB	3DDB	Kumaran et al., 2008
type C; apo form (zinc removed)	1.95 Å	3DEB	3DEB	3DEB	3DEB	3DEB	3DEB
mature peptidase	1.76 Å	3DS9	3DS9	3DS9	3DS9	3DS9	3DS9	Zuniga et al., 2008
a potent peptidomimetic inhibitor of botulinum neurotoxin serotype a has a very different conformation than snap-25 substrate	1.90 Å	3DSE	3DSE	3DSE	3DSE	3DSE	3DSE
domain organization in clostridium butulinum neurotoxin type e is unique: its implication in faster translocation	2.65 Å	3FFZ	3FFZ	3FFZ	3FFZ	3FFZ	3FFZ	Kumaran et al., 2009
mature peptidase	2.10 Å	3FIE	3FIE	3FIE	3FIE	3FIE	3FIE	Agarwal et al., 2009
mature peptidase	2.17 Å	3FII	3FII	3FII	3FII	3FII	3FII	Agarwal et al., 2009
mature peptidase	1.80 Å	3FUO	3FUO	3FUO	3FUO	3FUO	3FUO	Fu et al., 2009
catalytic domain; complex with a llama antibody	2.60 Å	3K3Q	3K3Q	3K3Q	3K3Q	3K3Q	3K3Q
mature peptidase	2.00 Å	3N7J	3N7J	3N7J	3N7J	3N7J	3N7J	Karalewitz et al., 2010
mature peptidase	2.50 Å	3N7K	3N7K	3N7K	3N7K	3N7K	3N7K	Karalewitz et al., 2010
crystal structure of bont/a lc with jth-nb-7239 peptide	2.40 Å	3NF3	3NF3	3NF3	3NF3	3NF3	3NF3
mature peptidase	1.72 Å	3OBR	3OBR	3OBR	3OBR	3OBR	3OBR	Strotmeier et al., 2010
mature peptidase	2.00 Å	3OBT	3OBT	3OBT	3OBT	3OBT	3OBT	Strotmeier et al., 2010
mature peptidase	1.65 Å	3OGG	3OGG	3OGG	3OGG	3OGG	3OGG	Zhang et al., 2010
crystal structure of bont/a lc with zinc bound	2.41 Å	3QIX	3QIX	3QIX	3QIX	3QIX	3QIX
complex with hydroxamate-based inhibitor pt-1	2.30 Å	3QIY	3QIY	3QIY	3QIY	3QIY	3QIY
complex with hydroxamate-based inhibitor pt-2	2.00 Å	3QIZ	3QIZ	3QIZ	3QIZ	3QIZ	3QIZ
complex with hydroxamate-based inhibitor pt-3	2.30 Å	3QJ0	3QJ0	3QJ0	3QJ0	3QJ0	3QJ0
crystal structure of the protease domain of botulinum neurotoxin serotype a with a peptide inhibitor rrgf	1.60 Å	3QW5	3QW5	3QW5	3QW5	3QW5	3QW5
crystal structure of the protease domain of botulinum neurotoxin serotype a with a peptide inhibitor rygc	1.60 Å	3QW6	3QW6	3QW6	3QW6	3QW6	3QW6
crystal structure of the protease domain of botulinum neurotoxin serotype a with a peptide inhibitor rrfc	1.50 Å	3QW7	3QW7	3QW7	3QW7	3QW7	3QW7
crystal structure of the protease domain of botulinum neurotoxin serotype a with a peptide inhibitor crgc	1.60 Å	3QW8	3QW8	3QW8	3QW8	3QW8	3QW8
mature peptidase	2.15 Å	3R4S	3R4S	3R4S	3R4S	3R4S	3R4S	Strotmeier et al., 2011
mature peptidase	2.20 Å	3R4U	3R4U	3R4U	3R4U	3R4U	3R4U	Strotmeier et al., 2011
mature peptidase	2.75 Å	3RMX	3RMX	3RMX	3RMX	3RMX	3RMX	Kroken et al., 2011
mature peptidase	2.30 Å	3RMY	3RMY	3RMY	3RMY	3RMY	3RMY	Kroken et al., 2011
mature peptidase	2.70 Å	3V0A	3V0A	3V0A	3V0A	3V0A	3V0A	Gu et al., 2012
mature peptidase	3.90 Å	3V0B	3V0B	3V0B	3V0B	3V0B	3V0B	Gu et al., 2012
mature peptidase	4.30 Å	3V0C	3V0C	3V0C	3V0C	3V0C	3V0C	Gu et al., 2012
mature peptidase	2.71 Å	3ZUR	3ZUR	3ZUR	3ZUR	3ZUR	3ZUR	Masuyer et al., 2011
mature peptidase	2.95 Å	3ZUS	3ZUS	3ZUS	3ZUS	3ZUS	3ZUS	Masuyer et al., 2011
mature peptidase	1.87 Å	4EJ5	4EJ5	4EJ5	4EJ5	4EJ5	4EJ5	Stura et al., 2012
mature peptidase	1.20 Å	4EL4	4EL4	4EL4	4EL4	4EL4	4EL4	Stura et al., 2012
mature peptidase	1.80 Å	4ELC	4ELC	4ELC	4ELC	4ELC	4ELC	Stura et al., 2012
mature peptidase	2.50 Å	4HEV	4HEV	4HEV	4HEV	4HEV	4HEV	Silhar et al., 2013
mature peptidase	2.30 Å	4IQP	4IQP	4IQP	4IQP	4IQP	4IQP	Przedpelski et al., 2013
mature peptidase	2.30 Å	4JRA	4JRA	4JRA	4JRA	4JRA	4JRA	Benoit et al., 2014
crystal structure of the catalytic domain of botulinum neurotoxin bont/a c134s mutant with covalent inhibitor that modifies cys-165 causing disorder in 166-174 stretch	1.93 Å	4KS6	4KS6	4KS6	4KS6	4KS6	4KS6
crystal structure of the catalytic domain of botulinum neurotoxin bont/a c134s mutant with covalent inhibitor that modifies cys-165 causing disorder in 167-174 stretch	2.59 Å	4KTX	4KTX	4KTX	4KTX	4KTX	4KTX
crystal structure of the catalytic domain of botulinum neurotoxin bont/a c134 mutant with mtsea modified cys-165 causing stretch disorder	1.70 Å	4KUF	4KUF	4KUF	4KUF	4KUF	4KUF
crystal structure of the catalytic domain of botulinum neurotoxin serotype a with a novel cyclic peptide inhibitor	1.94 Å	4ZJX	4ZJX	4ZJX	4ZJX	4ZJX	4ZJX
mature peptidase	3.10 Å	5BQM	5BQM	5BQM	5BQM	5BQM	5BQM	Masuyer et al., 2015
mature peptidase	2.30 Å	5BQN	5BQN	5BQN	5BQN	5BQN	5BQN	Masuyer et al., 2015
small molecule inhibitor abs-143 bound to the botulinum neurotoxin serotype a light chain	2.50 Å	5V8P	5V8P	5V8P	5V8P	5V8P	5V8P
small molecule inhibitor abs-143 bound to the botulinum neurotoxin serotype a light chain	1.90 Å	5V8R	5V8R	5V8R	5V8R	5V8R	5V8R
small molecule inhibitor abs-143 bound to the botulinum neurotoxin serotype a light chain	2.05 Å	5V8U	5V8U	5V8U	5V8U	5V8U	5V8U
structure of the c. botulinum neurotoxin serotype a with cu bound	2.60 Å	5VGV	5VGV	5VGV	5VGV	5VGV	5VGV
structure of the c. botulinum neurotoxin serotype a with hg bound	2.15 Å	5VGX	5VGX	5VGX	5VGX	5VGX	5VGX
Clostridium butyricum
serotype E catalytic domain; complex with snap-25 substrate peptide	2.25 Å	3D3X	3D3X	3D3X	3D3X	3D3X	3D3X	Agarwal & Swaminathan, 2008