Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 23 |
HIV-1 retropepsin |
16-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
HIV-1 retropepsin |
16-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
cathepsin K |
2-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
glutamyl endopeptidase I |
2-40 |
N |
|
MS |
Schilling & Overall, 2008 |
| 40 |
granzyme A |
1-451 |
N |
|
|
Van Damme et al., 2010 |
| 40 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 56 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 60 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 64 |
granzyme A |
1-451 |
N |
|
|
Van Damme et al., 2010 |
| 64 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 65 |
matrix metallopeptidase-2 |
53-83 |
N |
|
MS |
Schilling & Overall, 2008 |
| 65 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 66 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 67 |
HIV-1 retropepsin |
64-79 |
N |
|
MS |
Schilling & Overall, 2008 |
| 67 |
cathepsin G |
64-79 |
N |
|
MS |
Schilling & Overall, 2008 |
| 68 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 69 |
granzyme B (Homo sapiens-type) |
1-451 |
N |
|
MS |
Plasman et al., 2011 |
| 69 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 70 |
unknown peptidase |
1-451 |
P |
|
|
Van Damme et al., 2009 |
| 79 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 83 |
chymotrypsin A (cattle-type) |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
granzyme A |
1-451 |
N |
|
|
Van Damme et al., 2010 |
| 90 |
granzyme B (Homo sapiens-type) |
1-451 |
N |
|
MS |
Plasman et al., 2011 |
| 90 |
glutamyl endopeptidase I |
84-105 |
N |
|
MS |
Schilling & Overall, 2008 |
| 91 |
matrix metallopeptidase-2 |
84-105 |
N |
|
MS |
Schilling & Overall, 2008 |
| 92 |
cathepsin G |
84-105 |
N |
|
MS |
Schilling & Overall, 2008 |
| 92 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 93 |
elastase-2 |
84-105 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
cathepsin G |
84-105 |
N |
|
MS |
Schilling & Overall, 2008 |
| 95 |
peptidyl-Lys metallopeptidase |
1-451 |
N |
|
MS |
|
| 96 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 103 |
chymotrypsin A (cattle-type) |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 105 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
peptidyl-Lys metallopeptidase |
1-451 |
N |
|
MS |
|
| 123 |
peptidyl-Lys metallopeptidase |
1-451 |
N |
|
MS |
|
| 137 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 156 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 163 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 199 |
matrix metallopeptidase-2 |
166-214 |
N |
|
MS |
Schilling & Overall, 2008 |
| 200 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 202 |
HIV-1 retropepsin |
166-214 |
N |
|
MS |
Schilling & Overall, 2008 |
| 204 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 214 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 215 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 229 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 243 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 251 |
granzyme B (Homo sapiens-type) |
1-451 |
N |
|
MS |
Van Damme et al., 2009 |
| 252 |
HIV-1 retropepsin |
243-264 |
N |
|
MS |
Schilling & Overall, 2008 |
| 253 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 264 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 269 |
cathepsin G |
264-280 |
N |
|
MS |
Schilling & Overall, 2008 |
| 269 |
granzyme M |
1-451 |
P |
|
|
Bovenschen et al., 2008 |
| 270 |
matrix metallopeptidase-2 |
264-280 |
N |
|
MS |
Schilling & Overall, 2008 |
| 280 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 286 |
cathepsin G |
280-304 |
N |
|
MS |
Schilling & Overall, 2008 |
| 291 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 304 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 311 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 316 |
HIV-1 retropepsin |
311-326 |
N |
|
MS |
Schilling & Overall, 2008 |
| 320 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 325 |
peptidyl-Lys metallopeptidase |
1-451 |
N |
|
MS |
|
| 326 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 328 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 336 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 337 |
peptidyl-Lys metallopeptidase |
1-451 |
N |
|
MS |
|
| 339 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 342 |
HIV-1 retropepsin |
341-352 |
N |
|
MS |
Schilling & Overall, 2008 |
| 343 |
cathepsin G |
341-352 |
N |
|
MS |
Schilling & Overall, 2008 |
| 351 |
peptidyl-Lys metallopeptidase |
1-451 |
N |
|
MS |
|
| 352 |
granzyme A |
1-451 |
N |
|
|
Van Damme et al., 2010 |
| 352 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 353 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 355 |
elastase-2 |
352-370 |
N |
|
MS |
Schilling & Overall, 2008 |
| 369 |
peptidyl-Lys metallopeptidase |
1-451 |
N |
|
MS |
|
| 370 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 373 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 377 |
matrix metallopeptidase-2 |
373-390 |
N |
|
MS |
Schilling & Overall, 2008 |
| 390 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 394 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 401 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 407 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 413 |
HtrA2 peptidase |
1-451 |
P |
|
|
Vande Walle et al., 2007 |
| 422 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 430 |
trypsin 1 |
1-451 |
N |
|
MS |
Schilling & Overall, 2008 |
| 438 |
granzyme B (Homo sapiens-type) |
1-451 |
P |
|
|
Adrain et al., 2006 |
