Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 2 |
unknown peptidase |
1-377 |
P |
|
NT |
<%Agarwal et al., 2012[]%> |
| 19 |
HtrA2 peptidase |
3-377 |
P |
|
|
Vande Walle et al., 2007 |
| 20 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 30 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 41 |
thrombin |
27-59 |
N |
|
MS |
Schilling & Overall, 2008 |
| 42 |
matrix metallopeptidase-2 |
27-59 |
N |
|
MS |
Schilling & Overall, 2008 |
| 49 |
matrix metallopeptidase-2 |
33-67 |
N |
|
MS |
Schilling & Overall, 2008 |
| 51 |
peptidyl-Lys metallopeptidase |
1-377 |
N |
|
MS |
|
| 52 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 59 |
glutamyl endopeptidase I |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 62 |
peptidyl-Lys metallopeptidase |
1-377 |
N |
|
MS |
|
| 63 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 64 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 65 |
matrix metallopeptidase-2 |
64-74 |
N |
|
MS |
Schilling & Overall, 2008 |
| 67 |
chymotrypsin A (cattle-type) |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 69 |
peptidyl-Lys metallopeptidase |
1-377 |
N |
|
MS |
|
| 70 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 74 |
glutamyl endopeptidase I |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 85 |
peptidyl-Lys metallopeptidase |
1-377 |
N |
|
MS |
|
| 86 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 107 |
HtrA2 peptidase |
3-377 |
P |
|
|
Vande Walle et al., 2007 |
| 108 |
HtrA2 peptidase |
3-377 |
P |
|
|
Vande Walle et al., 2007 |
| 185 |
meprin alpha subunit |
3-377 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 185 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 193 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 221 |
HtrA2 peptidase |
3-377 |
P |
|
|
Vande Walle et al., 2007 |
| 228 |
glutamyl endopeptidase I |
227-240 |
N |
|
MS |
Schilling & Overall, 2008 |
| 229 |
HIV-1 retropepsin |
227-240 |
N |
|
MS |
Schilling & Overall, 2008 |
| 229 |
cathepsin G |
227-240 |
N |
|
MS |
Schilling & Overall, 2008 |
| 240 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 243 |
chymotrypsin A (cattle-type) |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 246 |
caspase-3 |
243-256 |
N |
|
MS |
Schilling & Overall, 2008 |
| 246 |
caspase-7 |
243-256 |
N |
|
MS |
Schilling & Overall, 2008 |
| 246 |
matrix metallopeptidase-2 |
243-257 |
N |
|
MS |
Schilling & Overall, 2008 |
| 246 |
glutamyl endopeptidase I |
243-256 |
N |
|
MS |
Schilling & Overall, 2008 |
| 256 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 257 |
chymotrypsin A (cattle-type) |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 301 |
cathepsin L |
294-314 |
N |
|
MS |
Biniossek et al., 2011 |
| 301 |
cathepsin B |
294-314 |
N |
|
MS |
Biniossek et al., 2011 |
| 303 |
astacin |
300-314 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 304 |
astacin |
301-314 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 306 |
cathepsin L |
294-314 |
N |
|
MS |
Biniossek et al., 2011 |
| 306 |
cathepsin S |
294-314 |
N |
|
MS |
Biniossek et al., 2011 |
| 316 |
peptidyl-Lys metallopeptidase |
1-377 |
N |
|
MS |
|
| 317 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
| 325 |
meprin alpha subunit |
3-377 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 325 |
meprin beta subunit |
3-377 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 327 |
peptidyl-Lys metallopeptidase |
1-377 |
N |
|
MS |
|
| 328 |
trypsin 1 |
3-377 |
N |
|
MS |
Schilling & Overall, 2008 |
