Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 52 |
cathepsin S |
43-64 |
N |
|
MS |
Biniossek et al., 2011 |
| 54 |
cathepsin B |
43-64 |
N |
|
MS |
Biniossek et al., 2011 |
| 54 |
matrix metallopeptidase-2 |
42-64 |
N |
|
MS |
Schilling & Overall, 2008 |
| 54 |
astacin |
51-64 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 54 |
meprin alpha subunit |
51-64 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 64 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 101 |
matrix metallopeptidase-2 |
92-118 |
N |
|
MS |
Schilling & Overall, 2008 |
| 118 |
glutamyl endopeptidase I |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 131 |
HIV-1 retropepsin |
127-148 |
N |
|
MS |
Schilling & Overall, 2008 |
| 148 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 209 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 220 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 232 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 244 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 249 |
LAST_MAM peptidase (Limulus-type) |
246-259 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 249 |
glutamyl endopeptidase I |
244-259 |
N |
|
MS |
Schilling & Overall, 2008 |
| 259 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 265 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 272 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 321 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 329 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 379 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 389 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 418 |
cathepsin B |
397-430 |
N |
|
MS |
Biniossek et al., 2011 |
| 421 |
cathepsin L |
397-445 |
N |
|
MS |
Biniossek et al., 2011 |
| 440 |
cathepsin S |
423-452 |
N |
|
MS |
Biniossek et al., 2011 |
| 440 |
cathepsin B |
397-452 |
N |
|
MS |
Biniossek et al., 2011 |
| 469 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 483 |
granzyme A |
1-1101 |
N |
|
|
Van Damme et al., 2010 |
| 483 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 521 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 528 |
HIV-1 retropepsin |
521-538 |
N |
|
MS |
Schilling & Overall, 2008 |
| 528 |
matrix metallopeptidase-2 |
521-538 |
N |
|
MS |
Schilling & Overall, 2008 |
| 538 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 592 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 607 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 614 |
matrix metallopeptidase-2 |
609-634 |
N |
|
MS |
Schilling & Overall, 2008 |
| 624 |
cathepsin B |
617-635 |
N |
|
MS |
Biniossek et al., 2011 |
| 624 |
meprin alpha subunit |
621-635 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 625 |
cathepsin B |
617-635 |
N |
|
MS |
Biniossek et al., 2011 |
| 625 |
matrix metallopeptidase-2 |
616-635 |
N |
|
MS |
Schilling & Overall, 2008 |
| 627 |
cathepsin L |
617-635 |
N |
|
MS |
Biniossek et al., 2011 |
| 627 |
cathepsin S |
617-635 |
N |
|
MS |
Biniossek et al., 2011 |
| 634 |
chymotrypsin A (cattle-type) |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 635 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 650 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 662 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 672 |
matrix metallopeptidase-2 |
669-683 |
N |
|
MS |
Schilling & Overall, 2008 |
| 676 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 683 |
glutamyl endopeptidase I |
676-703 |
N |
|
MS |
Schilling & Overall, 2008 |
| 686 |
cathepsin L |
677-697 |
N |
|
MS |
Biniossek et al., 2011 |
| 686 |
cathepsin S |
677-697 |
N |
|
MS |
Biniossek et al., 2011 |
| 703 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 716 |
cathepsin L |
712-726 |
N |
|
MS |
Biniossek et al., 2011 |
| 758 |
cathepsin G |
738-775 |
N |
|
MS |
Schilling & Overall, 2008 |
| 759 |
cathepsin L |
739-775 |
N |
|
MS |
Biniossek et al., 2011 |
| 759 |
cathepsin S |
739-775 |
N |
|
MS |
Biniossek et al., 2011 |
| 759 |
cathepsin B |
739-775 |
N |
|
MS |
Biniossek et al., 2011 |
| 775 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 835 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 839 |
matrix metallopeptidase-2 |
835-848 |
N |
|
MS |
Schilling & Overall, 2008 |
| 848 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 864 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 905 |
cathepsin S |
885-915 |
N |
|
MS |
Biniossek et al., 2011 |
| 918 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 923 |
matrix metallopeptidase-2 |
918-934 |
N |
|
MS |
Schilling & Overall, 2008 |
| 934 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 944 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 947 |
peptidyl-Lys metallopeptidase |
1-1101 |
N |
|
MS |
|
| 948 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 951 |
astacin |
948-962 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 961 |
peptidyl-Lys metallopeptidase |
1-1101 |
N |
|
MS |
|
| 962 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1002 |
matrix metallopeptidase-2 |
995-1012 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1012 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1056 |
matrix metallopeptidase-2 |
1045-1065 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1065 |
trypsin 1 |
1-1101 |
N |
|
MS |
Schilling & Overall, 2008 |
| 1068 |
cathepsin B |
1066-1077 |
N |
|
MS |
Biniossek et al., 2011 |
