Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 19 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 33 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 51 |
matrix metallopeptidase-2 |
41-67 |
N |
|
MS |
Schilling & Overall, 2008 |
| 67 |
chymotrypsin A (cattle-type) |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 73 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 89 |
meprin beta subunit |
86-102 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 90 |
glutamyl endopeptidase I |
84-102 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
cathepsin L |
85-102 |
N |
|
MS |
Biniossek et al., 2011 |
| 102 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 110 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 124 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 184 |
cathepsin G |
169-196 |
N |
|
MS |
Schilling & Overall, 2008 |
| 196 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 221 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 224 |
cathepsin S |
222-234 |
N |
|
MS |
Biniossek et al., 2011 |
| 234 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 240 |
HIV-1 retropepsin |
235-250 |
N |
|
MS |
Schilling & Overall, 2008 |
| 250 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 275 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 303 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 305 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 308 |
cathepsin S |
306-316 |
N |
|
MS |
Biniossek et al., 2011 |
| 316 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 334 |
meprin beta subunit |
330-346 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 342 |
cathepsin L |
333-352 |
N |
|
MS |
Biniossek et al., 2011 |
| 360 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 374 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 381 |
matrix metallopeptidase-2 |
378-392 |
N |
|
MS |
Schilling & Overall, 2008 |
| 388 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 390 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 392 |
chymotrypsin A (cattle-type) |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 403 |
matrix metallopeptidase-2 |
396-412 |
N |
|
MS |
Schilling & Overall, 2008 |
| 405 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 412 |
glutamyl endopeptidase I |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 499 |
cathepsin L |
484-517 |
N |
|
MS |
Biniossek et al., 2011 |
| 521 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 532 |
cathepsin S |
522-543 |
N |
|
MS |
Biniossek et al., 2011 |
| 541 |
matrix metallopeptidase-2 |
521-559 |
N |
|
MS |
Schilling & Overall, 2008 |
| 543 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 557 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 559 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 595 |
meprin alpha subunit |
1-840 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 595 |
meprin beta subunit |
1-840 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 651 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 668 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 697 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 704 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 717 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 723 |
LAST_MAM peptidase (Limulus-type) |
720-733 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 727 |
unknown peptidase |
|
P |
|
MS |
Mahrus et al., 2008 |
| 733 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 735 |
peptidyl-Lys metallopeptidase |
1-840 |
N |
|
MS |
|
| 736 |
lysyl endopeptidase (bacteria) |
1-840 |
N |
|
MS |
|
| 747 |
peptidyl-Lys metallopeptidase |
1-840 |
N |
|
MS |
|
| 748 |
lysyl endopeptidase (bacteria) |
1-840 |
N |
|
MS |
|
| 750 |
matrix metallopeptidase-2 |
743-767 |
N |
|
MS |
Schilling & Overall, 2008 |
| 754 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 764 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 767 |
glutamyl endopeptidase I |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 774 |
glutamyl endopeptidase I |
773-789 |
N |
|
MS |
Schilling & Overall, 2008 |
| 781 |
matrix metallopeptidase-2 |
774-796 |
N |
|
MS |
Schilling & Overall, 2008 |
| 789 |
trypsin 1 |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
| 796 |
glutamyl endopeptidase I |
1-840 |
N |
|
MS |
Schilling & Overall, 2008 |
