Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-556 |
P |
release of an initiating methionine |
MS |
Gevaert et al., 2006 |
| 18 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 23 |
cathepsin G |
18-33 |
N |
|
MS |
Schilling & Overall, 2008 |
| 30 |
matrix metallopeptidase-2 |
15-42 |
N |
|
MS |
Schilling & Overall, 2008 |
| 33 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 41 |
chymotrypsin A (cattle-type) |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 42 |
glutamyl endopeptidase I |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 43 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 62 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 63 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 72 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 73 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 83 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 84 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 101 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 102 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 110 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 111 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 116 |
matrix metallopeptidase-2 |
106-127 |
N |
|
MS |
Schilling & Overall, 2008 |
| 122 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 125 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 127 |
glutamyl endopeptidase I |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 130 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 135 |
matrix metallopeptidase-2 |
131-148 |
N |
|
MS |
Schilling & Overall, 2008 |
| 145 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 148 |
chymotrypsin A (cattle-type) |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 208 |
cathepsin B |
204-227 |
N |
|
MS |
Biniossek et al., 2011 |
| 218 |
cathepsin B |
204-227 |
N |
|
MS |
Biniossek et al., 2011 |
| 232 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 233 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 242 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 243 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 247 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 251 |
cathepsin L |
248-264 |
N |
|
MS |
Biniossek et al., 2011 |
| 253 |
elastase-2 |
247-264 |
N |
|
MS |
Schilling & Overall, 2008 |
| 264 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 298 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 309 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 342 |
HIV-1 retropepsin |
321-355 |
N |
|
MS |
Schilling & Overall, 2008 |
| 343 |
matrix metallopeptidase-2 |
321-355 |
N |
|
MS |
Schilling & Overall, 2008 |
| 346 |
cathepsin S |
322-355 |
N |
|
MS |
Biniossek et al., 2011 |
| 355 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 365 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 406 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 433 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 443 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 455 |
cathepsin L |
444-466 |
N |
|
MS |
Biniossek et al., 2011 |
| 455 |
cathepsin S |
444-466 |
N |
|
MS |
Biniossek et al., 2011 |
| 466 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 471 |
cathepsin L |
469-481 |
N |
|
MS |
Biniossek et al., 2011 |
| 471 |
cathepsin S |
469-481 |
N |
|
MS |
Biniossek et al., 2011 |
| 476 |
meprin beta subunit |
473-481 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 483 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 487 |
HIV-1 retropepsin |
484-496 |
N |
|
MS |
Schilling & Overall, 2008 |
| 487 |
cathepsin S |
485-496 |
N |
|
MS |
Biniossek et al., 2011 |
| 496 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 497 |
matrix metallopeptidase-2 |
490-514 |
N |
|
MS |
Schilling & Overall, 2008 |
| 498 |
peptidyl-Lys metallopeptidase |
1-556 |
N |
|
MS |
|
| 514 |
chymotrypsin A (cattle-type) |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 515 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
| 526 |
trypsin 1 |
1-556 |
N |
|
MS |
Schilling & Overall, 2008 |
