Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 7 |
cathepsin S |
1-18 |
N |
|
MS |
Biniossek et al., 2011 |
| 8 |
meprin alpha subunit |
5-23 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 11 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 23 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 33 |
meprin alpha subunit |
30-42 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 33 |
LAST_MAM peptidase (Limulus-type) |
30-42 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 35 |
HIV-1 retropepsin |
24-42 |
N |
|
MS |
Schilling & Overall, 2008 |
| 42 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 52 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 100 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 107 |
cathepsin L |
101-126 |
N |
|
MS |
Biniossek et al., 2011 |
| 113 |
cathepsin L |
101-126 |
N |
|
MS |
Biniossek et al., 2011 |
| 113 |
cathepsin S |
101-126 |
N |
|
MS |
Biniossek et al., 2011 |
| 125 |
glutamyl endopeptidase I |
101-135 |
N |
|
MS |
Schilling & Overall, 2008 |
| 126 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 135 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 149 |
cathepsin K |
145-158 |
N |
|
MS |
Schilling & Overall, 2008 |
| 158 |
astacin |
155-168 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 158 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 168 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 247 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 253 |
matrix metallopeptidase-2 |
250-263 |
N |
|
MS |
Schilling & Overall, 2008 |
| 256 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 263 |
glutamyl endopeptidase I |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 266 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 275 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 288 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 297 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 325 |
astacin |
322-326 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 326 |
cathepsin K |
321-336 |
N |
|
MS |
Schilling & Overall, 2008 |
| 336 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 338 |
glutamyl endopeptidase I |
337-350 |
N |
|
MS |
Schilling & Overall, 2008 |
| 350 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 353 |
cathepsin L |
351-364 |
N |
|
MS |
Biniossek et al., 2011 |
| 364 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 372 |
matrix metallopeptidase-2 |
364-381 |
N |
|
MS |
Schilling & Overall, 2008 |
| 376 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 384 |
glutamyl endopeptidase I |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 398 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 408 |
cathepsin B |
405-418 |
N |
|
MS |
Biniossek et al., 2011 |
| 411 |
cathepsin S |
405-420 |
N |
|
MS |
Biniossek et al., 2011 |
| 411 |
cathepsin B |
405-418 |
N |
|
MS |
Biniossek et al., 2011 |
| 504 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 518 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 522 |
HIV-1 retropepsin |
519-530 |
N |
|
MS |
Schilling & Overall, 2008 |
| 530 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 547 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 557 |
HIV-1 retropepsin |
548-573 |
N |
|
MS |
Schilling & Overall, 2008 |
| 573 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 587 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 594 |
trypsin 1 |
1-630 |
N |
|
MS |
Schilling & Overall, 2008 |
| 602 |
cathepsin L |
600-613 |
N |
|
MS |
Biniossek et al., 2011 |
| 602 |
meprin alpha subunit |
599-613 |
N |
|
MS |
Becker-Pauly et al., 2011 |
