Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 22 |
glutamyl endopeptidase I |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 25 |
HIV-1 retropepsin |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 26 |
matrix metallopeptidase-2 |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 27 |
glutamyl endopeptidase I |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 33 |
elastase-2 |
19-46 |
N |
|
MS |
Schilling & Overall, 2008 |
| 46 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 62 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 63 |
matrix metallopeptidase-2 |
59-81 |
N |
|
MS |
Schilling & Overall, 2008 |
| 77 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 81 |
chymotrypsin A (cattle-type) |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 82 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 83 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 84 |
elastase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 89 |
matrix metallopeptidase-2 |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 89 |
cathepsin G |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 90 |
cathepsin G |
77-103 |
N |
|
MS |
Schilling & Overall, 2008 |
| 103 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 108 |
cathepsin K |
104-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 108 |
glutamyl endopeptidase I |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 109 |
HIV-1 retropepsin |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
HIV-1 retropepsin |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
matrix metallopeptidase-2 |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 111 |
glutamyl endopeptidase I |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 112 |
HIV-1 retropepsin |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 112 |
cathepsin G |
104-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 113 |
elastase-2 |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
HIV-1 retropepsin |
104-121 |
N |
|
MS |
Schilling & Overall, 2008 |
| 114 |
caspase-3 |
104-122 |
N |
|
MS |
Schilling & Overall, 2008 |
| 121 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 122 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 136 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 137 |
cathepsin G |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 138 |
matrix metallopeptidase-2 |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 139 |
HtrA2 peptidase |
1-444 |
P |
|
|
Vande Walle et al., 2007 |
| 144 |
glutamyl endopeptidase I |
122-154 |
N |
|
MS |
Schilling & Overall, 2008 |
| 154 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 162 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 173 |
matrix metallopeptidase-2 |
172-183 |
N |
|
MS |
Schilling & Overall, 2008 |
| 174 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 183 |
chymotrypsin A (cattle-type) |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 186 |
matrix metallopeptidase-2 |
177-198 |
N |
|
MS |
Schilling & Overall, 2008 |
| 198 |
glutamyl endopeptidase I |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 224 |
glutamyl endopeptidase I |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 227 |
matrix metallopeptidase-2 |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 231 |
matrix metallopeptidase-2 |
223-241 |
N |
|
MS |
Schilling & Overall, 2008 |
| 241 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 251 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 252 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 262 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 282 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 297 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 309 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 318 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 324 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 335 |
peptidyl-Lys metallopeptidase |
1-444 |
N |
|
MS |
|
| 336 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 339 |
matrix metallopeptidase-2 |
337-350 |
N |
|
MS |
Schilling & Overall, 2008 |
| 349 |
peptidyl-Lys metallopeptidase |
1-444 |
N |
|
MS |
|
| 350 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 359 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 380 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 390 |
trypsin 1 |
1-444 |
N |
|
MS |
Schilling & Overall, 2008 |
| 392 |
lysyl endopeptidase (bacteria) |
1-444 |
N |
|
MS |
|
