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Literature for peptidase S01.156: enteropeptidase

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: A Assay, S Structure, I Inhibitor, K Knockout, P Specificity, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2026
  1. Ashok,Y., Bubb,K.L., Oy,C., Gorjifard,S., Cuperus,J.T., Queitsch,C. and Fields,S.
    Dosa: A method to covalently barcode proteins for high-throughput biochemistry
    Proc Natl Acad Sci U S A (2026) 123, e2529762123-e2529762123. PubMed  Europe PubMed DOI  PMC  EPMC
    2. 2025
  2. Akanksha, Tripathi,M., Mukherjee,K.J. and Pandey,G.
    An effective strategy for soluble bovine enterokinase expression in Escherichia coli
    Biotechnol Lett (2025) 47, 120-120. PubMed  Europe PubMed DOI
    3. 2024
  3. Elia Venanzi,N.A., Basciu,A., Vargiu,A.V., Kiparissides,A., Dalby,P.A. and Dikicioglu,D.
    Machine Learning Integrating Protein Structure, Sequence, and Dynamics to Predict the Enzyme Activity of Bovine Enterokinase Variants
    J Chem Inf Model (2024) 64, 2681-2694. PubMed  Europe PubMed DOI  PMC  EPMC
    4. 2023
  4. Chen,Y., Li,Z., Liu,C. and Wang,S.
    Enterokinase deficiency with novel TMPRSS15 gene mutation masquerading as acrodermatitis enteropathica
    Pediatr Dermatol (2023) 40, 389-391. PubMed  Europe PubMed DOI
  5. Kelly,C.J., Verdegaal,A.A., Anderson,B.W., Shaw,W.L., Bencivenga-Barry,N.A., Folta-Stogniew,E. and Goodman,A.L.
    Metformin inhibits digestive proteases and impairs protein digestion in mice
    J Biol Chem (2023) 299, 105363-105363. PubMed  Europe PubMed DOI  PMC  EPMC
    6. 2022
  6. Ayat,H., Darvishi,O., Moazeni,E. and Momeni Bidezard,A.
    Comparison of Periplasmic and Cytoplasmic Expression of Bovine Enterokinase Light Chain in E. coli
    Protein J (2022) 41, 157-165. PubMed  Europe PubMed DOI
  7. Yang,X., Ding,Z., Peng,L., Song,Q., Zhang,D., Cui,F., Xia,C., Li,K., Yin,H., Li,S., Li,Z. and Huang,H.
    Cryo-EM structures reveal the activation and substrate recognition mechanism of human enteropeptidase
    Nat Commun (2022) 13, 6955-6955. PubMed  Europe PubMed DOI  PMC  EPMC
    8. 2020
  8. Wang,L., Zhang,D., Fan,C., Zhou,X., Liu,Z., Zheng,B., Zhu,L. and Jin,Y.
    Novel Compound Heterozygous TMPRSS15 Gene Variants Cause Enterokinase Deficiency
    Front Genet (2020) 11, 538778-538778. PubMed  Europe PubMed DOI  PMC  EPMC
  9. Willard,F.S., Meredith,T.D., Showalter,A.D., Ma,W., Ho,J.D., Sauder,J.M. and Sloop,K.W.
    Synthetic protease-activated class B GPCRs
    Biochem Biophys Res Commun (2020) 530, 246-251. PubMed  Europe PubMed DOI
    10. 2018
  10. Hayashi,H., Kubo,Y., Izumida,M., Takahashi,E., Kido,H., Sato,K., Yamaya,M., Nishimura,H., Nakayama,K. and Matsuyama,T.
    Enterokinase enhances influenza A virus infection by activating trypsinogen in human cell lines
    Front Cell Infect Microbiol (2018) 8, 91-91. PubMed  Europe PubMed DOI  PMC  EPMC
  11. Wang,H., Li,S., Wang,J., Chen,S., Sun,X.L. and Wu,Q.
    N-glycosylation in the protease domain of trypsin-like serine proteases mediates calnexin-assisted protein folding
    elife (2018) 7 PubMed  Europe PubMed DOI
  12. Wang,Z., Guo,C., Liu,L. and Huang,H.
    Effects of N-glycosylation on the biochemical properties of recombinant bEKL expressed in Pichia pastoris
    Enzyme Microb Technol (2018) 114, 40-47. PubMed  Europe PubMed DOI  E
    13. 2016
  13. Luengo-Gil,G., Calvo,M.I., Martin-Villar,E., Aguila,S., Bohdan,N., Anton,A.I., Espin,S., Ayala de la Pena,F., Vicente,V., Corral,J., Quintanilla,M. and Martinez-Martinez,I.
    Antithrombin controls tumor migration, invasion and angiogenesis by inhibition of enteropeptidase
    Sci Rep (2016) 6, 27544-27544. PubMed  Europe PubMed DOI  PMC  EPMC
    14. 2014
  14. Xu,J., Hu,S., Wang,X., Zhao,Z., Zhang,X., Wang,H., Zhang,D. and Guo,Y.
    Structure basis for the unique specificity of medaka enteropeptidase light chain
    Protein Cell (2014) 5, 178-181. PubMed  Europe PubMed DOI  PMC  EPMC
    15. 2013
  15. Chen,Z., Han,S., Cao,Z., Wu,Y., Zhuo,R. and Li,W.
    Fusion expression and purification of four disulfide-rich peptides reveals enterokinase secondary cleavage sites in animal toxins
    Peptides (2013) 39, 145-151. PubMed  Europe PubMed DOI  P
  16. Skala,W., Goettig,P. and Brandstetter,H.
    Do-it-yourself histidine-tagged bovine enterokinase: A handy member of the protein engineer's toolbox
    J Biotechnol (2013) 168, 421-425. PubMed  Europe PubMed DOI  E
  17. Smith,E.T. and Johnson,D.A.
    Human enteropeptidase light chain: Bioengineering of recombinants and kinetic investigations of structure and function
    Protein Sci (2013) 22, 577-585. PubMed  Europe PubMed DOI  E
    18. 2012
  18. Pepeliaev,S., Krahulec,J., Tlusta,M., Cerny,Z. and Jilkova,J.
    Expression and purification of the light chain of human enteropeptidase in E. coli
    Minerva Biotecnol (2012) 24, 42-52.  E
  19. Prohaska,T.A., Wahlmuller,F.C., Furtmuller,M. and Geiger,M.
    Interaction of protein C inhibitor with the type II transmembrane serine protease enteropeptidase
    PLoS ONE (2012) 7, e39262-e39262. PubMed  Europe PubMed DOI  PMC  EPMC
  20. Sadler,J.E.
    Enteropeptidase
    [ISSN:978-0-12-407742-3] (2012) 3, 2648-2653. DOI
  21. Simeonov,P., Zahn,M., Strater,N. and Zuchner,T.
    Crystal structure of a supercharged variant of the human enteropeptidase light chain
    Proteins (2012) 80, 1907-1910. PubMed  Europe PubMed DOI  S
    22. 2011
  22. Antalis,T.M., Bugge,T.H. and Wu,Q.
    Membrane-anchored serine proteases in health and disease
    Prog Mol Biol Transl Sci (2011) 99, 1-50. PubMed  Europe PubMed DOI  V
  23. Choi,M.G., Lee,E., Chung,H.S., Jang,S.H. and Lee,C.
    A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin
    BMB Rep (2011) 44, 458-461. PubMed  Europe PubMed DOI  A
  24. Chun,H., Joo,K., Lee,J. and Shin,H.C.
    Design and efficient production of bovine enterokinase light chain with higher specificity in E. coli
    Biotechnol Lett (2011) 33, 1227-1232. PubMed  Europe PubMed DOI  E
  25. Gasparian,M.E., Bychkov,M.L., Dolgikh,D.A. and Kirpichnikov,M.P.
    Strategy for improvement of enteropeptidase efficiency in tag removal processes
    Protein Expr Purif (2011) 79, 191-196. PubMed  Europe PubMed DOI
  26. Pepeliaev,S., Krahulec,J., Cerny,Z., Jilkova,J., Tlusta,M. and Dostalova,J.
    High level expression of human enteropeptidase light chain in Pichia pastoris
    J Biotechnol (2011) 156, 67-75. PubMed  Europe PubMed DOI  E
  27. Schopfel,M., Tziridis,A., Arnold,U. and Stubbs,M.T.
    Towards a restriction proteinase: construction of a self-activating enzyme
    Chembiochem (2011) 12, 1523-1527. PubMed  Europe PubMed DOI
  28. Simeonov,P., Berger-Hoffmann,R., Hoffmann,R., Strater,N. and Zuchner,T.
    Surface supercharged human enteropeptidase light chain shows improved solubility and refolding yield
    Protein Eng Des Sel (2011) 24, 261-268. PubMed  Europe PubMed DOI  E
  29. Zauner,T., Berger-Hoffmann,R., Muller,K., Hoffmann,R. and Zuchner,T.
    Highly adaptable and sensitive protease assay based on fluorescence resonance energy transfer
    Anal Chem (2011) 83, 7356-7363. PubMed  Europe PubMed DOI  A
    30. 2009
  30. Zheng,X.L., Kitamoto,Y. and Sadler,J.E.
    Enteropeptidase, a type II transmembrane serine protease
    Front Biosci (Elite Ed) (2009) 1, 242-249. PubMed  Europe PubMed  V
    31. 2008
  31. Cui,T., Gao,Y., Ang,C.X., Puah,C.M., Gutte,B. and Lam,Y.
    Hydrogen peroxide enhances enterokinase-catalysed proteolytic cleavage of fusion protein
    Recent Pat Biotechnol (2008) 2, 189-190. PubMed  Europe PubMed
  32. Mikhailova,A.G., Likhareva,V.V. and Rumsh,L.D.
    A rate-limiting step of enteropeptidase hydrolysis
    Russ J Bioorganic Chem (2008) 34, 186-191. DOI
    33. 2007
  33. Ogiwara,K. and Takahashi,T.
    Specificity of the medaka enteropeptidase serine protease and its usefulness as a biotechnological tool for fusion-protein cleavage
    Proc Natl Acad Sci U S A (2007) 104, 7021-7026. PubMed  Europe PubMed DOI  PMC  EPMC
    34. 2006
  34. [YEAR:3-5-2006]Boulware,K.T. and Daugherty,P.S.
    Protease specificity determination by using cellular libraries of peptide substrates (CLiPS)
    Proc Natl Acad Sci U S A (3-5-2006) 103, 7583-7588. PubMed  Europe PubMed DOI  PMC  EPMC  P
    35. 2004
  35. Mikhailova,A.G., Likhareva,V.V., Vaskovsky,B.V., Garanin,S.K., Onoprienko,L.V., Prudchenko,I.A., Chikin,L.D. and Rumsh,L.D.
    Study of secondary specificity of enteropeptidase in comparison with trypsin
    Biochemistry (Mosc) (2004) 69, 909-917. PubMed  Europe PubMed DOI  P
  36. Sadler,J.E.
    Enteropeptidase
    [ISSN:0-12-079610-4] (2004) 2, 1513-1517.  V
    37. 2003
  37. Gasparian,M.E., Ostapchenko,V.G., Schulga,A.A., Dolgikh,D.A. and Kirpichnikov,M.P.
    Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli
    Protein Expr Purif (2003) 31, 133-139. PubMed  Europe PubMed DOI  E
  38. Imamura,T. and Kitamoto,Y.
    Expression of enteropeptidase in differentiated enterocytes, goblet cells, and the tumor cells in human duodenum
    Am J Physiol Gastrointest Liver Physiol (2003) 285, G1235-G1241. PubMed  Europe PubMed DOI
  39. Szabo,R., Wu,Q., Dickson,R.B., Netzel-Arnett,S., Antalis,T.M. and Bugge,T.H.
    Type II transmembrane serine proteases
    Thromb Haemost (2003) 90, 185-193. PubMed  Europe PubMed DOI  V
    40. 2002
  40. Holzinger,A., Maier,E.M., Buck,C., Mayerhofer,P.U., Kappler,M., Haworth,J.C., Moroz,S.P., Hadorn,H.B., Sadler,J.E. and Roscher,A.A.
    Mutations in the proenteropeptidase gene are the molecular cause of congenital enteropeptidase deficiency
    Am J Hum Genet (2002) 70, 20-25. PubMed  Europe PubMed DOI  PMC  EPMC  K
  41. [YEAR:1-4-2002]Song,H.W., Choi,S.I. and Seong,B.L.
    Engineered recombinant enteropeptidase catalytic subunit: Effect of N-terminal modification
    Arch Biochem Biophys (1-4-2002) 400, 1-6. PubMed  Europe PubMed DOI
  42. [YEAR:1-3-2002]Zheng,X. and Sadler,J.E.
    Mucin-like domain of enteropeptidase directs apical targeting in Madin-Darby canine kidney cells
    J Biol Chem (1-3-2002) 277, 6858-6863. PubMed  Europe PubMed DOI
    43. 2001
  43. Moroz,S.P., Hadorn,B., Rossi,T.M. and Haworth,J.C.
    Celiac disease in a patient with a congenital deficiency of intestinal enteropeptidase
    Am J Gastroenterol (2001) 96, 2251-2254. PubMed  Europe PubMed DOI
  44. Safi,W., Maiorano,J.N. and Davidson,W.S.
    A proteolytic method for distinguishing between lipid-free and lipid-bound apolipoprotein A-I
    J Lipid Res (2001) 42, 864-872. PubMed  Europe PubMed
    45. 2000
  45. Mikhailova,A.G. and Rumsh,L.D.
    Enteropeptidase - structure, function, and application in biotechnology
    Appl Biochem Biotechnol (2000) 88, 159-174.
  46. [YEAR:28-1-2000]Zamolodchikova,T.S., Sokolova,E.A., Lu,D. and Sadler,J.E.
    Activation of recombinant proenteropeptidase by duodenase
    FEBS Lett (28-1-2000) 466, 295-299. PubMed  Europe PubMed DOI
    47. 1999
  47. [YEAR:10-4-1999]Hosfield,T. and Lu,Q.
    Influence of the amino acid residue downstream of (Asp)4Lys on enterokinase cleavage of a fusion protein
    Anal Biochem (10-4-1999) 269, 10-16. PubMed  Europe PubMed DOI
  48. [YEAR:17-9-1999]Lu,D., Futterer,K., Korolev,S., Zheng,X., Tan,K., Waksman,G. and Sadler,J.E.
    Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide
    J Mol Biol (17-9-1999) 292, 361-373. PubMed  Europe PubMed DOI  S
  49. Matsushima,M., Ichinose,M., Yahagi,N., Tsukada-Kato,S., Miki,K., Omata,M., Kim,Y.T., Ito,H., Takahashi,T., Sakurai,Y., Tsuchiya,Y., Athauda,S.B., Inoue,H. and Takahashi,K.
    Purification and further characterization of enteropeptidase from porcine duodenum
    J Biochem (1999) 125, 947-951. PubMed  Europe PubMed  I
  50. [YEAR:15-1-1999]Mikhailova,A.G. and Rumsh,L.D.
    Autolysis of bovine enteropeptidase heavy chain: evidence of fragment 118-465 involvement in trypsinogen activation
    FEBS Lett (15-1-1999) 442, 226-230. PubMed  Europe PubMed DOI
  51. [YEAR:15-1-1999]Zheng,X., Lu,D. and Sadler,J.E.
    Apical sorting of bovine enteropeptidase does not involve detergent-resistant association with sphingolipid-cholesterol rafts
    J Biol Chem (15-1-1999) 274, 1596-1605. PubMed  Europe PubMed DOI
    52. 1998
  52. Yuan,X., Zheng,X., Lu,D., Rubin,D.C., Pung,C.Y. and Sadler,J.E.
    Structure of murine enterokinase (enteropeptidase) and expression in small intestine during development
    Am J Physiol (1998) 274, G342-G349. PubMed  Europe PubMed
    53. 1997
  53. [YEAR:12-12-1997]Lu,D., Yuan,X., Zheng,X. and Sadler,J.E.
    Bovine proenteropeptidase is activated by trypsin, and the specificity of enteropeptidase depends on the heavy chain
    J Biol Chem (12-12-1997) 272, 31293-31300. PubMed  Europe PubMed DOI
  54. Zamolodchikova,T.S., Sokolova,E.A., Alexandrov,S.L., Mikhaleva,I.I., Prudchenko,I.A., Morozov,I.A., Kononenko,N.V., Mirgorodskaya,O.A., Da,U., Larionova,N.I., Pozdnev,V.F., Ghosh,D., Duax,W.L. and Vorotyntseva,T.I.
    Subcellular localization, substrate specificity and crystallization of duodenase, a potential activator of enteropeptidase
    Eur J Biochem (1997) 249, 612-621. PubMed  Europe PubMed
    55. 1996
  55. Yahagi,N., Ichinose,M., Matsushima,M., Matsubara,Y., Miki,K., Kurokawa,K., Fukamachi,H., Tashiro,K., Shiokawa,K., Kageyama,T., Takahashi,T., Inoue,H. and Takahashi,K.
    Complementary DNA cloning and sequencing of rat enteropeptidase and tissue distribution of its mRNA
    Biochem Biophys Res Commun (1996) 219, 806-812. PubMed  Europe PubMed DOI
    56. 1995
  56. Kitamoto,Y., Veile,R.A., Donis-Keller,H. and Sadler,J.E.
    cDNA sequence and chromosomal localization of human enterokinase, the proteolytic activator of trypsinogen
    Biochemistry (1995) 34, 4562-4568. PubMed  Europe PubMed
    57. 1994
  57. Kitamoto,Y., Yuan,X., Wu,Q., McCourt,D.W. and Sadler,J.E.
    Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains
    Proc Natl Acad Sci U S A (1994) 91, 7588-7592. PubMed  Europe PubMed DOI  PMC  EPMC
  58. Mann,N.S. and Mann,S.K.
    Enterokinase
    Proc Soc Exp Biol Med (1994) 206, 114-118. PubMed  Europe PubMed  K
  59. Matsushima,M., Ichinose,M., Yahagi,N., Kakei,N., Tsukada,S., Miki,K., Kurokawa,K., Tashiro,K., Shiokawa,K. and Shinomiya,K.
    Structural characterization of porcine enteropeptidase
    J Biol Chem (1994) 269, 19976-19982. PubMed  Europe PubMed
    60. 1993
  60. LaVallie,E.R., Rehemtulla,A., Racie,L.A., DiBlasio,E.A., Ferenz,C., Grant,K.L., Light,A. and McCoy,J.M.
    Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase
    J Biol Chem (1993) 268, 23311-23317. PubMed  Europe PubMed
    61. 1991
  61. Light,A. and Janska,H.
    The amino-terminal sequence of the catalytic subunit of bovine enterokinase
    J Protein Chem (1991) 10, 475-480. PubMed  Europe PubMed
    62. 1989
  62. Light,A. and Janska,H.
    Enterokinase (enteropeptidase): comparative aspects
    Trends Biochem Sci (1989) 14, 110-112. PubMed  Europe PubMed
    63. 1987
  63. Jeno,P., Green,J.R. and Lentze,M.J.
    Specificity studies on enteropeptidase substrates related to the N-terminus of trypsinogen
    Biochem J (1987) 241, 721-727. PubMed  Europe PubMed  PMC  EPMC
    64. 1983
  64. Ghishan,F.K., Lee,P.C., Lebenthal,E., Johnson,P., Bradley,C.A. and Greene,H.L.
    Isolated congenital enterokinase deficiency. Recent findings and review of the literature
    Gastroenterology (1983) 85, 727-731. PubMed  Europe PubMed  K
    65. 1981
  65. Magee,A.I., Grant,D.A. and Taylor,J.H.
    Further studies on the subunit structure and oligosaccharide moiety of human enterokinase
    Clin Chim Acta (1981) 115, 241-254. PubMed  Europe PubMed
    66. 1978
  66. Grant,D.A., Magee,A.I. and Hermon-Taylor,J.
    Optimisation of conditions for the affinity chromatography of human enterokinase on immobilised p-aminobenzamidine. Improvement of the preparative procedure by inclusion of negative affinity chromatography with glycylglycyl-aniline
    Eur J Biochem (1978) 88, 183-189. PubMed  Europe PubMed  I
    67. 1976
  67. Grant,D.A. and Hermon-Taylor,J.
    The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme
    Biochem J (1976) 155, 243-254. PubMed  Europe PubMed  PMC  EPMC
    68. 1975
  68. Hadorn,B., Haworth,J.C., Gourley,B., Prasad,A. and Troesch,V.
    Intestinal enterokinase deficiency. Occurrence in two sibs and age dependency of clinical expression
    Arch Dis Child (1975) 50, 277-282. PubMed  Europe PubMed  PMC  EPMC  K
    69. 1974
  69. Rinderknecht,H., Engeling,E.R., Bunnell,M.J. and Geokas,M.C.
    A sensitive assay for human enterokinase and some properties of the enzyme
    Clin Chim Acta (1974) 54, 145-160. PubMed  Europe PubMed
    70. 1973
  70. Baratti,J., Maroux,S. and Louvard,D.
    Effect of ionic strength and calcium ions on the activation of trypsinogen by enterokinase. A modified test for the quantitative evaluation of this enzyme
    Biochim Biophys Acta (1973) 321, 632-638. PubMed  Europe PubMed
    71. 1971
  71. Maroux,S., Baratti,J. and Desnuelle,P.
    Purification and specificity of porcine enterokinase
    J Biol Chem (1971) 246, 5031-5039. PubMed  Europe PubMed
    72. 1970
  72. Nordstrom,C. and Dahlqvist,A.
    The cellular localization of enterokinase
    Biochim Biophys Acta (1970) 198, 621-622. PubMed  Europe PubMed
    73. 1969
  73. [YEAR:19-4-1969]Hadorn,B., Tarlow,M.J., Lloyd,J.K. and Wolff,O.H.
    Intestinal enterokinase deficiency
    Lancet (19-4-1969) 1, 812-813. PubMed  Europe PubMed  K
    74. 1956
  74. Yamashina,I.
    The action of enterokinase on trypsinogen
    Biochim Biophys Acta (1956) 20, 433-434. PubMed  Europe PubMed
    75. 1955
  75. Laskowski,M.
    [3] Trypsinogen and trypsin
    Methods Enzymol (1955) 2, 26-36. DOI  V
    76. 1954
  76. Yamashina,I.
    Studies on enterokinase. I. The purification and general properties
    Ark Kemi (1954) 7, 539-543.
  77. Yamashina,I.
    Studies on enterokinase. II. The further purification
    Ark Kemi (1954) 9, 225-229.
    78. 1939
  78. [YEAR:20-3-1939]Kunitz,M.
    Purification and concentration of enterokinase
    J Gen Physiol (20-3-1939) 22, 447-450. PubMed  Europe PubMed DOI
  79. [YEAR:20-3-1939]Kunitz,M.
    Formation of trypsin from crystalline trypsinogen by means of enterokinase
    J Gen Physiol (20-3-1939) 22, 429-446. PubMed  Europe PubMed DOI
    80. 1924
  80. Waldschmidt-Leitz,E.
    Uber Enterokinase
    Naturwissenschaften (1924) 12, 133-137. DOI
    81. 1913
  81. Mellanby,J. and Woolley,V.J.
    The ferments of the pancreas: Part III. The properties of trypsin, trypsinogen and enterokinase
    J Physiol (1913) 47, 339-360. PubMed  Europe PubMed DOI
    82. 1912
  82. Mellanby,J. and Woolley,V.J.
    The ferments of the pancreas: part I. The generation of trypsin from trypsinogen by enterokinase
    J Physiol (1912) 45, 370-388. PubMed  Europe PubMed DOI
    83. 1906
  83. Hamill,J.M.
    On the mechanism of protection of intestinal worms, and its bearing on the relation of enterokinase to trypsin
    J Physiol (1906) 33, 479-492. PubMed  Europe PubMed DOI

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