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Literature for peptidase C54.003: autophagin-1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, A Assay, T Target, P Specificity, I Inhibitor, K Knockout, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Cheng,X., Xia,T., Liang,G., Xu,H.D., Liu,X. and Liang,G.
    Atg4B-Activated Near-Infrared Nanoprobe for Precise Imaging of Autophagy In Vivo
    Anal Chem (2025) 97, 21181-21186. PubMed  Europe PubMed DOI
  2. Ryan,P.J., Guerra,B.C., Uranga,S., Cardin,J.M., Riechman,S.E., Gomes,M.J. and Fluckey,J.D.
    ATG4B is required for mTORC1-mediated anabolic activity and is associated with clinical outcomes in non-small cell lung cancer
    FEBS Open Bio (2025) PubMed  Europe PubMed DOI
  3. Yang,F., Li,S., Bi,X., Yuan,R. and Xiang,Y.
    Highly Sensitive and Specific Detection of Autophagy Signaling Pathway in Live Cancer Cells for Chemoresistance Assessment via ZnO-DNA Nanosphere Probes
    Anal Chem (2025) 97, 24859-24866. PubMed  Europe PubMed DOI
    4. 2024
  4. Zhang,N., Shen,M.Y., Meng,Q.L., Sun,H.P., Fan,F.Y., Yi,H. and Yang,Y.J.
    FAT1 inhibits AML autophagy and proliferation via downregulating ATG4B expression
    Biochim Biophys Acta Gen Subj (2024) 1868, 130519-130519. PubMed  Europe PubMed DOI  K
    5. 2023
  5. Kim,B.H., Kwon,D.H., Kim,L., Yang,W.S. and Song,H.K.
    In vitro production of N-degron fused proteins and its application
    Methods Enzymol (2023) 686, 99-123. PubMed  Europe PubMed DOI
  6. Pilger,D.R.B., Luft,C. and Ketteler,R.
    Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase
    J Vis Exp (2023) PubMed  Europe PubMed DOI
    7. 2022
  7. Kudo,Y., Endo,S., Fujita,M., Ota,A., Kamatari,Y.O., Tanaka,Y., Ishikawa,T., Ikeda,H., Okada,T., Toyooka,N., Fujimoto,N., Matsunaga,T. and Ikari,A.
    Discovery and Structure-Based Optimization of Novel Atg4B Inhibitors for the Treatment of Castration-Resistant Prostate Cancer
    J Med Chem (2022) 65, 4878-4892. PubMed  Europe PubMed DOI  I
  8. Li,W., Luo,L.X., Zhou,Q.Q., Gong,H.B., Fu,Y.Y., Yan,C.Y., Li,E., Sun,J., Luo,Z., Ding,Z.J., Zhang,Q.Y., Mu,H.L., Cao,Y.F., Ouyang,S.H., Kurihara,H., Li,Y.F., Sun,W.Y., Li,M. and He,R.R.
    Phospholipid peroxidation inhibits autophagy via stimulating the delipidation of oxidized LC3-PE
    Redox Biol (2022) 55, 102421-102421. PubMed  Europe PubMed DOI  PMC  EPMC
  9. Liu,P.F., Chen,C.F., Ger,L.P., Tsai,W.L., Tseng,H.H., Lee,C.H., Yang,W.H. and Shu,C.W.
    MAP3K11 facilitates autophagy activity and is correlated with malignancy of oral squamous cell carcinoma
    J Cell Physiol (2022) 237, 4275-4291. PubMed  Europe PubMed DOI  K
  10. Park,N.Y., Jo,D.S. and Cho,D.H.
    Post-Translational Modifications of ATG4B in the Regulation of Autophagy
    Cells (2022) 11 PubMed  Europe PubMed DOI  PMC  EPMC
  11. Tang,Y., Kay,A., Jiang,Z. and Arkin,M.R.
    LC3B Binds to the Autophagy Protease ATG4b with High Affinity Using a Bipartite Interface
    Biochemistry (2022) 61, 2295-2302. PubMed  Europe PubMed DOI  PMC  EPMC
    12. 2021
  12. Ligeon,L.A., Pena-Francesch,M., Vanoaica,L.D., Nunez,N.G., Talwar,D., Dick,T.P. and Munz,C.
    Oxidation inhibits autophagy protein deconjugation from phagosomes to sustain MHC class II restricted antigen presentation
    Nat Commun (2021) 12, 1508-1508. PubMed  Europe PubMed DOI  PMC  EPMC
  13. Martinez-Garcia,G.G., Perez,R.F., Fernandez,A.F., Durand,S., Kroemer,G. and Marino,G.
    Autophagy Deficiency by Atg4B Loss Leads to Metabolomic Alterations in Mice
    Metabolites (2021) 11 PubMed  Europe PubMed DOI  PMC  EPMC
    14. 2020
  14. Fu,Y., Gu,Q., Luo,L., Xu,J., Luo,Y., Xia,F., Han,F., Hong,L., Yin,X.M., Huang,Z. and Li,M.
    New Anti-Cancer Strategy to Suppress Colorectal Cancer Growth Through Inhibition of ATG4B and Lysosome Function
    Cancers (Basel) (2020) 12 PubMed  Europe PubMed DOI  PMC  EPMC
  15. Li,Y., Wang,B., Zhu,Y., Tian,Z., Yang,Z., Duan,J. and Wang,Z.
    The cysteine protease ATG4B of Trichinella spiralis promotes larval invasion into the intestine of the host
    Vet Res (2020) 51, 69-69. PubMed  Europe PubMed DOI  PMC  EPMC
  16. Zheng,X., Yang,Z., Gu,Q., Xia,F., Fu,Y., Liu,P., Yin,X.M. and Li,M.
    The protease activity of human ATG4B is regulated by reversible oxidative modification
    Autophagy (2020) , 1-13. PubMed  Europe PubMed DOI
    17. 2019
  17. Agrotis,A. and Ketteler,R.
    On ATG4B as drug target for treatment of solid tumours - the knowns and the unknowns
    Cells (2019) 9 PubMed  Europe PubMed DOI  T
  18. Fu,Y., Hong,L., Xu,J., Zhong,G., Gu,Q., Gu,Q., Guan,Y., Zheng,X., Dai,Q., Luo,X., Liu,C., Huang,Z., Yin,X.M., Liu,P. and Li,M.
    Discovery of a small molecule targeting autophagy via ATG4B inhibition and cell death of colorectal cancer cells in vitro and in vivo
    Autophagy (2019) 15, 295-311. PubMed  Europe PubMed DOI  T
  19. Shin,J.H., Park,S.J., Jo,D.S., Park,N.Y., Kim,J.B., Bae,J.E., Jo,Y.K., Hwang,J.J., Lee,J.A., Jo,D.G., Kim,J.C., Jung,Y.K., Koh,J.Y. and Cho,D.H.
    Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation
    Autophagy (2019) 15, 1495-1505. PubMed  Europe PubMed DOI
  20. Sun,Y., Zheng,Q., Wang,Y., Pang,Z., Liu,J., Yin,Z. and Lou,Z.
    Activity-based protein profiling identifies ATG4B as a key host factor for EV71 proliferation
    J Virol (2019) 93, e01092-19-e01092-19. PubMed  Europe PubMed DOI
  21. Tanc,M., Cleenewerck,M., Kurdi,A., Roelandt,R., Declercq,W., De Meyer,G., Augustyns,K., Martinet,W. and Van der Veken,P.
    Synthesis and evaluation of novel benzotropolones as Atg4B inhibiting autophagy blockers
    Bioorg Chem (2019) 87, 163-168. PubMed  Europe PubMed DOI  I
    22. 2018
  22. Bosc,D., Vezenkov,L., Bortnik,S., An,J., Xu,J., Choutka,C., Hannigan,A.M., Kovacic,S., Loo,S., Clark,P.G.K., Chen,G., Guay-Ross,R.N., Yang,K., Dragowska,W.H., Zhang,F., Go,N.E., Leung,A., Honson,N.S., Pfeifer,T.A., Gleave,M., Bally,M., Jones,S.J., Gorski,S.M. and Young,R.N.
    A new quinoline-based chemical probe inhibits the autophagy-related cysteine protease ATG4B
    Sci Rep (2018) 8, 11653-11653. PubMed  Europe PubMed DOI  PMC  EPMC  I
  23. Chu,J., Fu,Y., Xu,J., Zheng,X., Gu,Q., Luo,X., Dai,Q., Zhang,S., Liu,P., Hong,L. and Li,M.
    ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition
    Arch Biochem Biophys (2018) 644, 29-36. PubMed  Europe PubMed DOI  I
  24. Kauffman,K.J., Yu,S., Jin,J., Mugo,B., Nguyen,N., O'Brien,A., Nag,S., Lystad,A.H. and Melia,T.J.
    Delipidation of mammalian Atg8-family proteins by each of the four ATG4 proteases
    Autophagy (2018) 14, 992-1010. PubMed  Europe PubMed DOI
    25. 2017
  25. Kjos,I., Borg Distefano,M., Saetre,F., Repnik,U., Holland,P., Jones,A.T., Engedal,N., Simonsen,A., Bakke,O. and Progida,C.
    Rab7b modulates autophagic flux by interacting with Atg4B
    EMBO Rep (2017) 18, 1727-1739. PubMed  Europe PubMed DOI
  26. Liu,P.F., Hsu,C.J., Tsai,W.L., Cheng,J.S., Chen,J.J., Huang,I.F., Tseng,H.H., Chang,H.W. and Shu,C.W.
    Ablation of ATG4B suppressed autophagy and activated AMPK for cell cycle arrest in cancer cells
    Cell Physiol Biochem (2017) 44, 728-740. PubMed  Europe PubMed DOI  K
  27. Maruyama,T. and Noda,N.N.
    Autophagy-regulating protease Atg4: structure, function, regulation and inhibition
    J Antibiot (Tokyo) (2017) 71, 72-78. PubMed  Europe PubMed DOI
  28. Skytte Rasmussen,M., Mouilleron,S., Kumar Shrestha,B., Wirth,M., Lee,R., Bowitz Larsen,K., Abudu Princely,Y., O'Reilly,N., Sjottem,E., Tooze,S.A., Lamark,T. and Johansen,T.
    ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8
    Autophagy (2017) 13, 834-853. PubMed  Europe PubMed DOI
  29. Xu,D., Xu,Z., Han,L., Liu,C., Zhou,Z., Qiu,Z., Lin,X., Tang,G., Shen,H., Aebi,J., Riemer,C., Kuhn,B., Stahl,M., Mark,D., Qin,N. and Ding,H.
    Identification of new ATG4B inhibitors based on a novel high-throughput screening platform
    J Biomol Screen (2017) 22, 338-347. PubMed  Europe PubMed DOI  A  I
    30. 2016
  30. Bortnik,S., Choutka,C., Horlings,H.M., Leung,S., Baker,J.H., Lebovitz,C., Dragowska,W.H., Go,N.E., Bally,M.B., Minchinton,A.I., Gelmon,K.A. and Gorski,S.M.
    Identification of breast cancer cell subtypes sensitive to ATG4B inhibition
    Oncotarget (2016) 7, 66970-66988. PubMed  Europe PubMed DOI  T
  31. Qiu,Z., Kuhn,B., Aebi,J., Lin,X., Ding,H., Zhou,Z., Xu,Z., Xu,D., Han,L., Liu,C., Qiu,H., Zhang,Y., Haap,W., Riemer,C., Stahl,M., Qin,N., Shen,H.C. and Tang,G.
    Discovery of fluoromethylketone-based peptidomimetics as covalent ATG4B (autophagin-1) inhibitors
    ACS Med Chem Lett (2016) 7, 802-806. PubMed  Europe PubMed DOI  PMC  EPMC  I
  32. Seo,E., Woo,J., Park,E., Bertolani,S.J., Siegel,J.B., Choi,D. and Dinesh-Kumar,S.P.
    Comparative analyses of ubiquitin-like ATG8 and cysteine protease ATG4 autophagy genes in the plant lineage and cross-kingdom processing of ATG8 by ATG4
    Autophagy (2016) 12, 2054-2068. PubMed  Europe PubMed DOI  P
    33. 2015
  33. Cabrera,S., Maciel,M., Herrera,I., Nava,T., Vergara,F., Gaxiola,M., Lopez-Otin,C., Selman,M. and Pardo,A.
    Essential role for the ATG4B protease and autophagy in bleomycin-induced pulmonary fibrosis
    Autophagy (2015) 11, 670-684. PubMed  Europe PubMed DOI  K
  34. Fernandez,AF. and Lopez-Otin,C.
    The functional and pathologic relevance of autophagy proteases
    J Clin Invest (2015) 125, 33-41. PubMed  Europe PubMed DOI  V
  35. Vezenkov,L., Honson,N.S., Kumar,N.S., Bosc,D., Kovacic,S., Nguyen,T.G., Pfeifer,T.A. and Young,R.N.
    Development of fluorescent peptide substrates and assays for the key autophagy-initiating cysteine protease enzyme, ATG4B
    Bioorg Med Chem (2015) 23, 3237-3247. PubMed  Europe PubMed DOI  A
  36. Yang,Z., Wilkie-Grantham,R.P., Yanagi,T., Shu,C.W., Matsuzawa,S. and Reed,J.C.
    ATG4B (autophagin-1) phosphorylation modulates autophagy
    J Biol Chem (2015) 290, 26549-26561. PubMed  Europe PubMed DOI
    37. 2014
  37. Akin,D., Wang,S.K., Habibzadegah-Tari,P., Law,B., Ostrov,D., Li,M., Yin,X.M., Kim,J.S., Horenstein,N. and Dunn,W.A., Jr.
    A novel ATG4B antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors
    Autophagy (2014) 10, 2021-2035. PubMed  Europe PubMed DOI  I
  38. Nguyen,T.G., Honson,N.S., Arns,S., Davis,T.L., Dhe-Paganon,S., Kovacic,S., Kumar,N.S., Pfeifer,T.A. and Young,R.N.
    Development of fluorescent substrates and assays for the key autophagy-related cysteine protease enzyme, ATG4B
    Assay Drug Dev Technol (2014) 12, 176-189. PubMed  Europe PubMed DOI  A  P
    39. 2013
  39. Cabrera,S., Fernandez,A.F., Marino,G., Aguirre,A., Suarez,M.F., Espanol,Y., Vega,J.A., Laura,R., Fueyo,A., Soledad Fernandez-Garcia,M., Freije,J.M.P., Kroemer,G. and Lopez-Otin,C.
    ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis
    Autophagy (2013) 9, 1188-1200. PubMed  Europe PubMed DOI  K
  40. Liu,C., Ma,H., Wu,J., Huang,Q., Liu,J.O. and Yu,L.
    Arginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins
    BMC Cell Biol (2013) 14, 27-27. PubMed  Europe PubMed DOI  P
    41. 2011
  41. Li,M., Hou,Y., Wang,J., Chen,X., Shao,Z.M. and Yin,X.M.
    Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates
    J Biol Chem (2011) 286, 7327-7338. PubMed  Europe PubMed DOI  P
  42. Read,R., Savelieva,K., Baker,K., Hansen,G. and Vogel,P.
    Histopathological and neurological features of Atg4b knockout mice
    Vet Pathol (2011) 48, 486-494. PubMed  Europe PubMed DOI  K
  43. Shu,C.W., Madiraju,C., Zhai,D., Welsh,K., Diaz,P., Sergienko,E., Sano,R. and Reed,J.C.
    High-throughput fluorescence assay for small-molecule inhibitors of autophagins/Atg4
    J Biomol Screen (2011) 16, 174-182. PubMed  Europe PubMed DOI  A
    44. 2010
  44. Marino,G., Fernandez,A.F., Cabrera,S., Lundberg,Y.W., Cabanillas,R., Rodriguez,F., Salvador-Montoliu,N., Vega,J.A., Germana,A., Fueyo,A., Freije,J.M. and Lopez-Otin,C.
    Autophagy is essential for mouse sense of balance
    J Clin Invest (2010) 120, 2331-2344. PubMed  Europe PubMed DOI  K
  45. Shu,C.W., Drag,M., Bekes,M., Zhai,D., Salvesen,G.S. and Reed,J.C.
    Synthetic substrates for measuring activity of autophagy proteases: autophagins (Atg4)
    Autophagy (2010) 6, 936-947. PubMed  Europe PubMed DOI  A
  46. Till,A. and Subramani,S.
    A balancing act for autophagin
    J Clin Invest (2010) 120, 2273-2276. PubMed  Europe PubMed DOI  V
    47. 2009
  47. Fujita,N., Noda,T. and Yoshimori,T.
    Atg4B(C74A) hampers autophagosome closure: a useful protein for inhibiting autophagy
    Autophagy (2009) 5, 88-89. PubMed  Europe PubMed
  48. Satoo,K., Noda,N.N., Kumeta,H., Fujioka,Y., Mizushima,N., Ohsumi,Y. and Inagaki,F.
    The structure of Atg4B-LC3 complex reveals the mechanism of LC3 processing and delipidation during autophagy
    EMBO J (2009) 28, 1341-1350. PubMed  Europe PubMed DOI  PMC  EPMC  S
    49. 2008
  49. Fujita,N., Hayashi-Nishino,M., Fukumoto,H., Omori,H., Yamamoto,A., Noda,T. and Yoshimori,T.
    An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure
    Mol Biol Cell (2008) 19, 4651-4659. PubMed  Europe PubMed DOI  PMC  EPMC
    50. 2007
  50. [YEAR:1-2-2007]Satoo,K., Suzuki,N.N., Fujioka,Y., Mizushima,N., Ohsumi,Y. and Inagaki,F.
    Crystallization and preliminary crystallographic analysis of human Atg4B-LC3 complex
    Acta Crystallograph Sect F Struct Biol Cryst Commun (1-2-2007) 63, 99-102. PubMed  Europe PubMed DOI  PMC  EPMC
    51. 2006
  51. [YEAR:28-11-2006]Kumanomidou,T., Mizushima,T., Komatsu,M., Suzuki,A., Tanida,I., Sou,Y.S., Ueno,T., Kominami,E., Tanaka,K. and Yamane,T.
    The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers
    J Mol Biol (28-11-2006) 355, 612-618. PubMed  Europe PubMed DOI  S
  52. Yoshimura,K., Shibata,M., Koike,M., Gotoh,K., Fukaya,M., Watanabe,M. and Uchiyama,Y.
    Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues
    Autophagy (2006) 2, 200-208. PubMed  Europe PubMed  K
    53. 2005
  53. [YEAR:23-9-2005]Sugawara,K., Suzuki,N.N., Fujioka,Y., Mizushima,N., Ohsumi,Y. and Inagaki,F.
    Structural basis for the specificity and catalysis of human Atg4B responsible for mammalian autophagy
    J Biol Chem (23-9-2005) 280, 40058-40065. PubMed  Europe PubMed DOI  S
    54. 2004
  54. Fricke,J., Voss,C., Thumm,M. and Meyers,G.
    Processing of a pestivirus protein by a cellular protease specific for light chain 3 of microtubule-associated proteins
    J Virol (2004) 78, 5900-5912. PubMed  Europe PubMed DOI  PMC  EPMC
  55. [YEAR:8-6-2004]Tanida,I., Sou,Y.S., Ezaki,J., Minematsu-Ikeguchi,N., Ueno,T. and Kominami,E.
    HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates
    J Biol Chem (8-6-2004) 279, 36268-36276. PubMed  Europe PubMed DOI
    56. 2003
  56. [YEAR:19-12-2003]Hemelaar,J., Lelyveld,V.S., Kessler,B.M. and Ploegh,H.L.
    A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L
    J Biol Chem (19-12-2003) 278, 51841-51850. PubMed  Europe PubMed DOI
  57. [YEAR:7-2-2003]Marino,G., Uria,J.A., Puente,X.S., Quesada,V., Bordallo,J. and Lopez-Otin,C.
    Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy
    J Biol Chem (7-2-2003) 278, 3671-3678. PubMed  Europe PubMed DOI
    58. 2001
  58. Thumm,M. and Kadowaki,T.
    The loss of Drosophila APG4/AUT2 function modifies the phenotypes of cut and Notch signaling pathway mutants
    Mol Genet Genomics (2001) 266, 657-663. PubMed  Europe PubMed DOI

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