Literature for peptidase C54.003: autophagin-1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, A Assay, T Target, P Specificity, I Inhibitor, K Knockout, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2025

Cheng,X., Xia,T., Liang,G., Xu,H.D., Liu,X. and Liang,G. Atg4B-Activated Near-Infrared Nanoprobe for Precise Imaging of Autophagy In Vivo Anal Chem (2025) 97, 21181-21186. PubMed Europe PubMed DOI
Ryan,P.J., Guerra,B.C., Uranga,S., Cardin,J.M., Riechman,S.E., Gomes,M.J. and Fluckey,J.D. ATG4B is required for mTORC1-mediated anabolic activity and is associated with clinical outcomes in non-small cell lung cancer FEBS Open Bio (2025) PubMed Europe PubMed DOI
Yang,F., Li,S., Bi,X., Yuan,R. and Xiang,Y. Highly Sensitive and Specific Detection of Autophagy Signaling Pathway in Live Cancer Cells for Chemoresistance Assessment via ZnO-DNA Nanosphere Probes Anal Chem (2025) 97, 24859-24866. PubMed Europe PubMed DOI

2024

Zhang,N., Shen,M.Y., Meng,Q.L., Sun,H.P., Fan,F.Y., Yi,H. and Yang,Y.J. FAT1 inhibits AML autophagy and proliferation via downregulating ATG4B expression Biochim Biophys Acta Gen Subj (2024) 1868, 130519-130519. PubMed Europe PubMed DOI K

2023

Kim,B.H., Kwon,D.H., Kim,L., Yang,W.S. and Song,H.K. In vitro production of N-degron fused proteins and its application Methods Enzymol (2023) 686, 99-123. PubMed Europe PubMed DOI
Pilger,D.R.B., Luft,C. and Ketteler,R. Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase J Vis Exp (2023) PubMed Europe PubMed DOI

2022

Kudo,Y., Endo,S., Fujita,M., Ota,A., Kamatari,Y.O., Tanaka,Y., Ishikawa,T., Ikeda,H., Okada,T., Toyooka,N., Fujimoto,N., Matsunaga,T. and Ikari,A. Discovery and Structure-Based Optimization of Novel Atg4B Inhibitors for the Treatment of Castration-Resistant Prostate Cancer J Med Chem (2022) 65, 4878-4892. PubMed Europe PubMed DOI I
Li,W., Luo,L.X., Zhou,Q.Q., Gong,H.B., Fu,Y.Y., Yan,C.Y., Li,E., Sun,J., Luo,Z., Ding,Z.J., Zhang,Q.Y., Mu,H.L., Cao,Y.F., Ouyang,S.H., Kurihara,H., Li,Y.F., Sun,W.Y., Li,M. and He,R.R. Phospholipid peroxidation inhibits autophagy via stimulating the delipidation of oxidized LC3-PE Redox Biol (2022) 55, 102421-102421. PubMed Europe PubMed DOI PMC EPMC
Liu,P.F., Chen,C.F., Ger,L.P., Tsai,W.L., Tseng,H.H., Lee,C.H., Yang,W.H. and Shu,C.W. MAP3K11 facilitates autophagy activity and is correlated with malignancy of oral squamous cell carcinoma J Cell Physiol (2022) 237, 4275-4291. PubMed Europe PubMed DOI K
Park,N.Y., Jo,D.S. and Cho,D.H. Post-Translational Modifications of ATG4B in the Regulation of Autophagy Cells (2022) 11 PubMed Europe PubMed DOI PMC EPMC
Tang,Y., Kay,A., Jiang,Z. and Arkin,M.R. LC3B Binds to the Autophagy Protease ATG4b with High Affinity Using a Bipartite Interface Biochemistry (2022) 61, 2295-2302. PubMed Europe PubMed DOI PMC EPMC

2021

Ligeon,L.A., Pena-Francesch,M., Vanoaica,L.D., Nunez,N.G., Talwar,D., Dick,T.P. and Munz,C. Oxidation inhibits autophagy protein deconjugation from phagosomes to sustain MHC class II restricted antigen presentation Nat Commun (2021) 12, 1508-1508. PubMed Europe PubMed DOI PMC EPMC
Martinez-Garcia,G.G., Perez,R.F., Fernandez,A.F., Durand,S., Kroemer,G. and Marino,G. Autophagy Deficiency by Atg4B Loss Leads to Metabolomic Alterations in Mice Metabolites (2021) 11 PubMed Europe PubMed DOI PMC EPMC

2020

Fu,Y., Gu,Q., Luo,L., Xu,J., Luo,Y., Xia,F., Han,F., Hong,L., Yin,X.M., Huang,Z. and Li,M. New Anti-Cancer Strategy to Suppress Colorectal Cancer Growth Through Inhibition of ATG4B and Lysosome Function Cancers (Basel) (2020) 12 PubMed Europe PubMed DOI PMC EPMC
Li,Y., Wang,B., Zhu,Y., Tian,Z., Yang,Z., Duan,J. and Wang,Z. The cysteine protease ATG4B of Trichinella spiralis promotes larval invasion into the intestine of the host Vet Res (2020) 51, 69-69. PubMed Europe PubMed DOI PMC EPMC
Zheng,X., Yang,Z., Gu,Q., Xia,F., Fu,Y., Liu,P., Yin,X.M. and Li,M. The protease activity of human ATG4B is regulated by reversible oxidative modification Autophagy (2020) , 1-13. PubMed Europe PubMed DOI

2019

Agrotis,A. and Ketteler,R. On ATG4B as drug target for treatment of solid tumours - the knowns and the unknowns Cells (2019) 9 PubMed Europe PubMed DOI T
Fu,Y., Hong,L., Xu,J., Zhong,G., Gu,Q., Gu,Q., Guan,Y., Zheng,X., Dai,Q., Luo,X., Liu,C., Huang,Z., Yin,X.M., Liu,P. and Li,M. Discovery of a small molecule targeting autophagy via ATG4B inhibition and cell death of colorectal cancer cells in vitro and in vivo Autophagy (2019) 15, 295-311. PubMed Europe PubMed DOI T
Shin,J.H., Park,S.J., Jo,D.S., Park,N.Y., Kim,J.B., Bae,J.E., Jo,Y.K., Hwang,J.J., Lee,J.A., Jo,D.G., Kim,J.C., Jung,Y.K., Koh,J.Y. and Cho,D.H. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation Autophagy (2019) 15, 1495-1505. PubMed Europe PubMed DOI
Sun,Y., Zheng,Q., Wang,Y., Pang,Z., Liu,J., Yin,Z. and Lou,Z. Activity-based protein profiling identifies ATG4B as a key host factor for EV71 proliferation J Virol (2019) 93, e01092-19-e01092-19. PubMed Europe PubMed DOI
Tanc,M., Cleenewerck,M., Kurdi,A., Roelandt,R., Declercq,W., De Meyer,G., Augustyns,K., Martinet,W. and Van der Veken,P. Synthesis and evaluation of novel benzotropolones as Atg4B inhibiting autophagy blockers Bioorg Chem (2019) 87, 163-168. PubMed Europe PubMed DOI I

2018

Bosc,D., Vezenkov,L., Bortnik,S., An,J., Xu,J., Choutka,C., Hannigan,A.M., Kovacic,S., Loo,S., Clark,P.G.K., Chen,G., Guay-Ross,R.N., Yang,K., Dragowska,W.H., Zhang,F., Go,N.E., Leung,A., Honson,N.S., Pfeifer,T.A., Gleave,M., Bally,M., Jones,S.J., Gorski,S.M. and Young,R.N. A new quinoline-based chemical probe inhibits the autophagy-related cysteine protease ATG4B Sci Rep (2018) 8, 11653-11653. PubMed Europe PubMed DOI PMC EPMC I
Chu,J., Fu,Y., Xu,J., Zheng,X., Gu,Q., Luo,X., Dai,Q., Zhang,S., Liu,P., Hong,L. and Li,M. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition Arch Biochem Biophys (2018) 644, 29-36. PubMed Europe PubMed DOI I
Kauffman,K.J., Yu,S., Jin,J., Mugo,B., Nguyen,N., O'Brien,A., Nag,S., Lystad,A.H. and Melia,T.J. Delipidation of mammalian Atg8-family proteins by each of the four ATG4 proteases Autophagy (2018) 14, 992-1010. PubMed Europe PubMed DOI

2017

Kjos,I., Borg Distefano,M., Saetre,F., Repnik,U., Holland,P., Jones,A.T., Engedal,N., Simonsen,A., Bakke,O. and Progida,C. Rab7b modulates autophagic flux by interacting with Atg4B EMBO Rep (2017) 18, 1727-1739. PubMed Europe PubMed DOI
Liu,P.F., Hsu,C.J., Tsai,W.L., Cheng,J.S., Chen,J.J., Huang,I.F., Tseng,H.H., Chang,H.W. and Shu,C.W. Ablation of ATG4B suppressed autophagy and activated AMPK for cell cycle arrest in cancer cells Cell Physiol Biochem (2017) 44, 728-740. PubMed Europe PubMed DOI K
Maruyama,T. and Noda,N.N. Autophagy-regulating protease Atg4: structure, function, regulation and inhibition J Antibiot (Tokyo) (2017) 71, 72-78. PubMed Europe PubMed DOI
Skytte Rasmussen,M., Mouilleron,S., Kumar Shrestha,B., Wirth,M., Lee,R., Bowitz Larsen,K., Abudu Princely,Y., O'Reilly,N., Sjottem,E., Tooze,S.A., Lamark,T. and Johansen,T. ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8 Autophagy (2017) 13, 834-853. PubMed Europe PubMed DOI
Xu,D., Xu,Z., Han,L., Liu,C., Zhou,Z., Qiu,Z., Lin,X., Tang,G., Shen,H., Aebi,J., Riemer,C., Kuhn,B., Stahl,M., Mark,D., Qin,N. and Ding,H. Identification of new ATG4B inhibitors based on a novel high-throughput screening platform J Biomol Screen (2017) 22, 338-347. PubMed Europe PubMed DOI A I

2016

Bortnik,S., Choutka,C., Horlings,H.M., Leung,S., Baker,J.H., Lebovitz,C., Dragowska,W.H., Go,N.E., Bally,M.B., Minchinton,A.I., Gelmon,K.A. and Gorski,S.M. Identification of breast cancer cell subtypes sensitive to ATG4B inhibition Oncotarget (2016) 7, 66970-66988. PubMed Europe PubMed DOI T
Qiu,Z., Kuhn,B., Aebi,J., Lin,X., Ding,H., Zhou,Z., Xu,Z., Xu,D., Han,L., Liu,C., Qiu,H., Zhang,Y., Haap,W., Riemer,C., Stahl,M., Qin,N., Shen,H.C. and Tang,G. Discovery of fluoromethylketone-based peptidomimetics as covalent ATG4B (autophagin-1) inhibitors ACS Med Chem Lett (2016) 7, 802-806. PubMed Europe PubMed DOI PMC EPMC I
Seo,E., Woo,J., Park,E., Bertolani,S.J., Siegel,J.B., Choi,D. and Dinesh-Kumar,S.P. Comparative analyses of ubiquitin-like ATG8 and cysteine protease ATG4 autophagy genes in the plant lineage and cross-kingdom processing of ATG8 by ATG4 Autophagy (2016) 12, 2054-2068. PubMed Europe PubMed DOI P

2015

Cabrera,S., Maciel,M., Herrera,I., Nava,T., Vergara,F., Gaxiola,M., Lopez-Otin,C., Selman,M. and Pardo,A. Essential role for the ATG4B protease and autophagy in bleomycin-induced pulmonary fibrosis Autophagy (2015) 11, 670-684. PubMed Europe PubMed DOI K
Fernandez,AF. and Lopez-Otin,C. The functional and pathologic relevance of autophagy proteases J Clin Invest (2015) 125, 33-41. PubMed Europe PubMed DOI V
Vezenkov,L., Honson,N.S., Kumar,N.S., Bosc,D., Kovacic,S., Nguyen,T.G., Pfeifer,T.A. and Young,R.N. Development of fluorescent peptide substrates and assays for the key autophagy-initiating cysteine protease enzyme, ATG4B Bioorg Med Chem (2015) 23, 3237-3247. PubMed Europe PubMed DOI A
Yang,Z., Wilkie-Grantham,R.P., Yanagi,T., Shu,C.W., Matsuzawa,S. and Reed,J.C. ATG4B (autophagin-1) phosphorylation modulates autophagy J Biol Chem (2015) 290, 26549-26561. PubMed Europe PubMed DOI

2014

Akin,D., Wang,S.K., Habibzadegah-Tari,P., Law,B., Ostrov,D., Li,M., Yin,X.M., Kim,J.S., Horenstein,N. and Dunn,W.A., Jr. A novel ATG4B antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors Autophagy (2014) 10, 2021-2035. PubMed Europe PubMed DOI I
Nguyen,T.G., Honson,N.S., Arns,S., Davis,T.L., Dhe-Paganon,S., Kovacic,S., Kumar,N.S., Pfeifer,T.A. and Young,R.N. Development of fluorescent substrates and assays for the key autophagy-related cysteine protease enzyme, ATG4B Assay Drug Dev Technol (2014) 12, 176-189. PubMed Europe PubMed DOI A P

2013

Cabrera,S., Fernandez,A.F., Marino,G., Aguirre,A., Suarez,M.F., Espanol,Y., Vega,J.A., Laura,R., Fueyo,A., Soledad Fernandez-Garcia,M., Freije,J.M.P., Kroemer,G. and Lopez-Otin,C. ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis Autophagy (2013) 9, 1188-1200. PubMed Europe PubMed DOI K
Liu,C., Ma,H., Wu,J., Huang,Q., Liu,J.O. and Yu,L. Arginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins BMC Cell Biol (2013) 14, 27-27. PubMed Europe PubMed DOI P

2011

Li,M., Hou,Y., Wang,J., Chen,X., Shao,Z.M. and Yin,X.M. Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates J Biol Chem (2011) 286, 7327-7338. PubMed Europe PubMed DOI P
Read,R., Savelieva,K., Baker,K., Hansen,G. and Vogel,P. Histopathological and neurological features of Atg4b knockout mice Vet Pathol (2011) 48, 486-494. PubMed Europe PubMed DOI K
Shu,C.W., Madiraju,C., Zhai,D., Welsh,K., Diaz,P., Sergienko,E., Sano,R. and Reed,J.C. High-throughput fluorescence assay for small-molecule inhibitors of autophagins/Atg4 J Biomol Screen (2011) 16, 174-182. PubMed Europe PubMed DOI A

2010

Marino,G., Fernandez,A.F., Cabrera,S., Lundberg,Y.W., Cabanillas,R., Rodriguez,F., Salvador-Montoliu,N., Vega,J.A., Germana,A., Fueyo,A., Freije,J.M. and Lopez-Otin,C. Autophagy is essential for mouse sense of balance J Clin Invest (2010) 120, 2331-2344. PubMed Europe PubMed DOI K
Shu,C.W., Drag,M., Bekes,M., Zhai,D., Salvesen,G.S. and Reed,J.C. Synthetic substrates for measuring activity of autophagy proteases: autophagins (Atg4) Autophagy (2010) 6, 936-947. PubMed Europe PubMed DOI A
Till,A. and Subramani,S. A balancing act for autophagin J Clin Invest (2010) 120, 2273-2276. PubMed Europe PubMed DOI V

2009

Fujita,N., Noda,T. and Yoshimori,T. Atg4B(C74A) hampers autophagosome closure: a useful protein for inhibiting autophagy Autophagy (2009) 5, 88-89. PubMed Europe PubMed
Satoo,K., Noda,N.N., Kumeta,H., Fujioka,Y., Mizushima,N., Ohsumi,Y. and Inagaki,F. The structure of Atg4B-LC3 complex reveals the mechanism of LC3 processing and delipidation during autophagy EMBO J (2009) 28, 1341-1350. PubMed Europe PubMed DOI PMC EPMC S

2008

Fujita,N., Hayashi-Nishino,M., Fukumoto,H., Omori,H., Yamamoto,A., Noda,T. and Yoshimori,T. An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure Mol Biol Cell (2008) 19, 4651-4659. PubMed Europe PubMed DOI PMC EPMC

2007

[YEAR:1-2-2007]Satoo,K., Suzuki,N.N., Fujioka,Y., Mizushima,N., Ohsumi,Y. and Inagaki,F. Crystallization and preliminary crystallographic analysis of human Atg4B-LC3 complex Acta Crystallograph Sect F Struct Biol Cryst Commun (1-2-2007) 63, 99-102. PubMed Europe PubMed DOI PMC EPMC

2006

[YEAR:28-11-2006]Kumanomidou,T., Mizushima,T., Komatsu,M., Suzuki,A., Tanida,I., Sou,Y.S., Ueno,T., Kominami,E., Tanaka,K. and Yamane,T. The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers J Mol Biol (28-11-2006) 355, 612-618. PubMed Europe PubMed DOI S
Yoshimura,K., Shibata,M., Koike,M., Gotoh,K., Fukaya,M., Watanabe,M. and Uchiyama,Y. Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues Autophagy (2006) 2, 200-208. PubMed Europe PubMed K

2005

[YEAR:23-9-2005]Sugawara,K., Suzuki,N.N., Fujioka,Y., Mizushima,N., Ohsumi,Y. and Inagaki,F. Structural basis for the specificity and catalysis of human Atg4B responsible for mammalian autophagy J Biol Chem (23-9-2005) 280, 40058-40065. PubMed Europe PubMed DOI S

2004

Fricke,J., Voss,C., Thumm,M. and Meyers,G. Processing of a pestivirus protein by a cellular protease specific for light chain 3 of microtubule-associated proteins J Virol (2004) 78, 5900-5912. PubMed Europe PubMed DOI PMC EPMC
[YEAR:8-6-2004]Tanida,I., Sou,Y.S., Ezaki,J., Minematsu-Ikeguchi,N., Ueno,T. and Kominami,E. HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates J Biol Chem (8-6-2004) 279, 36268-36276. PubMed Europe PubMed DOI

2003

[YEAR:19-12-2003]Hemelaar,J., Lelyveld,V.S., Kessler,B.M. and Ploegh,H.L. A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L J Biol Chem (19-12-2003) 278, 51841-51850. PubMed Europe PubMed DOI
[YEAR:7-2-2003]Marino,G., Uria,J.A., Puente,X.S., Quesada,V., Bordallo,J. and Lopez-Otin,C. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy J Biol Chem (7-2-2003) 278, 3671-3678. PubMed Europe PubMed DOI

2001

Thumm,M. and Kadowaki,T. The loss of Drosophila APG4/AUT2 function modifies the phenotypes of cut and Notch signaling pathway mutants Mol Genet Genomics (2001) 266, 657-663. PubMed Europe PubMed DOI