Merops Switch to
Inhibitors

Peptidase

Family Clan SEARCHES BLAST
MEROPS SUBMISSIONS

Other
information

Literature for peptidase C01.062: cathepsin B-like peptidase (platyhelminth)

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, K Knockout, P Specificity, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Holub,J., Jilkova,A., Lemke,C., Cianni,L., Spiwokova,P., Horn,M., Breuer,C., Leontovyc,A., Brynda,J., Mertlikova-Kaiserova,H., Chanova,M., Dos Reis Rocho,F., Montanari,C.A., El-Sakkary,N., Caffrey,C.R., Gutschow,M., Hnyk,D., Mares,M. and Fanfrlik,J.
    Fat or flat? The impact of dipole moment vectors on non-covalent interactions between aromatic tags and macromolecules
    Inorg Chem Front (2025) PubMed  Europe PubMed DOI  PMC  EPMC
    2. 2024
  2. Fuchs,N., Zimmermann,R.A., Schwickert,M., Gunkel,A., Zimmer,C., Meta,M., Schwickert,K., Keiser,J., Haeberli,C., Kiefer,W. and Schirmeister,T.
    Dual Strategy to Design New Agents Targeting Schistosoma mansoni: Advancing Phenotypic and SmCB1 Inhibitors for Improved Efficacy
    ACS Infect Dis (2024) 10, 1664-1678. PubMed  Europe PubMed DOI  I
  3. Nascimento,I., Albino,S., Menezes,K., Cavalcanti,M., Oliveira,M., Mali,S. and Moura,R.
    Targeting SmCB1: Perspectives and Insights to Design Antischistosomal Drugs
    Curr Med Chem (2024) 31, 2264-2284. PubMed  Europe PubMed DOI
  4. Spiwokova,P., Horn,M., Fanfrlik,J., Jilkova,A., Fajtova,P., Leontovyc,A., Houstecka,R., Bielikova,L., Brynda,J., Chanova,M., Mertlikova-Kaiserova,H., Caro-Diaz,E.J.E., Almaliti,J., El-Sakkary,N., Gerwick,W.H., Caffrey,C.R. and Mares,M.
    Nature-Inspired Gallinamides Are Potent Antischistosomal Agents: Inhibition of the Cathepsin B1 Protease Target and Binding Mode Analysis
    ACS Infect Dis (2024) 10, 1935-1948. PubMed  Europe PubMed DOI  I
    5. 2022
  5. Perera,D.J., Hassan,A.S., Liu,S.S., Elahi,S.M., Gadoury,C., Weeratna,R.D., Gilbert,R. and Ndao,M.
    A low dose adenovirus vectored vaccine expressing Schistosoma mansoni Cathepsin B protects from intestinal schistosomiasis in mice
    EBioMedicine (2022) 80, 104036-104036. PubMed  Europe PubMed DOI  PMC  EPMC
    6. 2020
  6. Dvorakova,H., Leontovyc,R., Machacek,T., O'Donoghue,A.J., Sedo,O., Zdrahal,Z., Craik,C.S., Caffrey,C.R., Horak,P. and Mikes,L.
    Isoforms of cathepsin B1 in neurotropic schistosomula of Trichobilharzia regenti differ in substrate preferences and a highly expressed catalytically inactive paralog binds cystatin
    Front Cell Infect Microbiol (2020) 10, 66-66. PubMed  Europe PubMed DOI  PMC  EPMC  P
  7. Jilkova,A., Horn,M. and Mares,M.
    Structural and functional characterization of Schistosoma mansoni cathepsin B1
    Methods Mol Biol (2020) 2151, 145-158. PubMed  Europe PubMed DOI  E  S  I
  8. Jilkova,A., Rubesova,P., Fanfrlik,J., Fajtova,P., Rezacova,P., Brynda,J., Lepsik,M., Mertlikova-Kaiserova,H., Emal,C.D., Renslo,A.R., Roush,W.R., Horn,M., Caffrey,C.R. and Mares,M.
    Druggable Hot Spots in the Schistosomiasis Cathepsin B1 Target Identified by Functional and Binding Mode Analysis of Potent Vinyl Sulfone Inhibitors
    ACS Infect Dis (2020) PubMed  Europe PubMed DOI
  9. Jilkova,A., Horn,M., Fanfrlik,J., Kuppers,J., Pachl,P., Rezacova,P., Lepsik,M., Fajtova,P., Rubesova,P., Chanova,M., Caffrey,C.R., Gutschow,M. and Mares,M.
    Azanitrile Inhibitors of the SmCB1 Protease Target Are Lethal to Schistosoma mansoni: Structural and Mechanistic Insights into Chemotype Reactivity
    ACS Infect Dis (2020) PubMed  Europe PubMed DOI
  10. Kianifard,L., Yakhchali,M. and Imani,M.
    The Effects of pH and Temperature on Cysteine Protease (Cathepsin B) Activity in Miracidia and Eggs of Fasciola hepatica
    Iran J Parasitol (2020) 15, 233-239. PubMed  Europe PubMed  PMC  EPMC
  11. Perera,D.J., Hassan,A.S., Jia,Y., Ricciardi,A., McCluskie,M.J., Weeratna,R.D. and Ndao,M.
    Adjuvanted Schistosoma mansoni-Cathepsin B With Sulfated Lactosyl Archaeol Archaeosomes or AddaVax Provides Protection in a Pre-Clinical Schistosomiasis Model
    Front Immunol (2020) 11, 605288-605288. PubMed  Europe PubMed DOI  PMC  EPMC
  12. Tallima,H., Abou El Dahab,M. and El Ridi,R.
    Specific Antibodies and Arachidonic Acid Mediate the Protection Induced by the Schistosoma mansoni Cysteine Peptidase-Based Vaccine in Mice
    Vaccines (Basel) (2020) 8 PubMed  Europe PubMed DOI  PMC  EPMC
    13. 2019
  13. Soloviova,K., Fox,E.C., Dalton,J.P., Caffrey,C.R. and Davies,S.J.
    A secreted schistosome cathepsin B1 cysteine protease and acute schistosome infection induce a transient T helper 17 response
    PLoS Negl Trop Dis (2019) 13, e0007070-e0007070. PubMed  Europe PubMed DOI
    14. 2015
  14. Chen,W., Ning,D., Wang,X., Chen,T., Lv,X., Sun,J., Wu,D., Huang,Y., Xu,J. and Yu,X.
    Identification and characterization of Clonorchis sinensis cathepsin B proteases in the pathogenesis of clonorchiasis
    Parasit Vectors (2015) 8, 647-647. PubMed  Europe PubMed DOI  PMC  EPMC
  15. Fonseca,N.C., da Cruz,L.F., da Silva Villela,F., do Nascimento Pereira,G.A., de Siqueira-Neto,J.L., Kellar,D., Suzuki,B.M., Ray,D., de Souza,T.B., Alves,R.J., Junior,P.A., Romanha,A.J., Murta,S.M., McKerrow,J.H., Caffrey,C.R., de Oliveira,R.B. and Ferreira,R.S.
    Synthesis of a sugar-based thiosemicarbazone series and structure-activity relationship versus the parasite cysteine proteases rhodesain, cruzain, and Schistosoma mansoni cathepsin B1
    Antimicrob Agents Chemother (2015) 59, 2666-2677. PubMed  Europe PubMed DOI  I
  16. Ricciardi,A., Dalton,J.P. and Ndao,M.
    Evaluation of the immune response and protective efficacy of Schistosoma mansoni cathepsin B in mice using CpG dinucleotides as adjuvant
    Vaccine (2015) 33, 346-353. PubMed  Europe PubMed DOI
  17. Siricoon,S., Vichasri Grams,S., Lertwongvisarn,K., Abdullohfakeeyah,M., Smooker,P.M. and Grams,R.
    Fasciola gigantica cathepsin B5 is an acidic endo- and exopeptidase of the immature and mature parasite
    Biochimie (2015) 119, 6-15. PubMed  Europe PubMed DOI
    18. 2014
  18. Chen,W., Wang,X., Lv,X., Tian,Y., Xu,Y., Mao,Q., Shang,M., Li,X., Huang,Y. and Yu,X.
    Characterization of the secreted cathepsin B cysteine proteases family of the carcinogenic liver fluke Clonorchis sinensis
    Parasitol Res (2014) 113, 3409-3418. PubMed  Europe PubMed DOI
  19. Dalton,J.P. and Dvorak,J.
    Activating the cathepsin B1 of a parasite: a major route with alternative pathways?
    Structure (2014) 22, 1696-1698. PubMed  Europe PubMed DOI  S
  20. El Ridi,R., Tallima,H., Dalton,J.P. and Donnelly,S.
    Induction of protective immune responses against schistosomiasis using functionally active cysteine peptidases
    Front Genet (2014) 5, 119-119. PubMed  Europe PubMed DOI  PMC  EPMC
  21. Jilkova,A., Horn,M., Rezacova,P., Maresova,L., Fajtova,P., Brynda,J., Vondrasek,J., McKerrow,J.H., Caffrey,C.R. and Mares,M.
    Activation route of the Schistosoma mansoni cathepsin B1 drug target: structural map with a glycosaminoglycan switch
    Structure (2014) 22, 1786-1798. PubMed  Europe PubMed DOI  S
    22. 2013
  22. Fanfrlik,J., Brahmkshatriya,P.S., Rezac,J., Jilkova,A., Horn,M., Mares,M., Hobza,P. and Lepsik,M.
    Quantum mechanics-based scoring rationalizes the irreversible inactivation of parasitic Schistosoma mansoni cysteine peptidase by vinyl sulfone inhibitors
    J Phys Chem B (2013) 117, 14973-14982. PubMed  Europe PubMed DOI  I
    23. 2012
  23. Robinson,M.W., Brindley,P.J., McKerrow,J.H. and Dalton,J.P.
    Trematode cysteine endopeptidases
    [ISSN:978-0-12-407743-0] (2012) 3, 1941-1949. DOI
  24. Sripa,J., Brindley,P.J., Sripa,B., Loukas,A., Kaewkes,S. and Laha,T.
    Evaluation of liver fluke recombinant cathepsin B-1 protease as a serodiagnostic antigen for human opisthorchiasis
    Parasitol Int (2012) 61, 191-195. PubMed  Europe PubMed DOI
    25. 2011
  25. Horn,M., Jilkova,A., Vondrasek,J., Maresova,L., Caffrey,C.R. and Mares,M.
    Mapping the pro-peptide of the Schistosoma mansoni cathepsin B1 drug target: modulation of inhibition by heparin and design of mimetic inhibitors
    ACS Chem Biol (2011) 6, 609-617. PubMed  Europe PubMed DOI
  26. Kasny,M., Mikes,L., Doleckova,K., Hampl,V., Dvorak,J., Novotny,M. and Horak,P.
    Cathepsins B1 and B2 of Trichobilharzia spp., bird schistosomes causing cercarial dermatitis
    Adv Exp Med Biol (2011) 712, 136-154. PubMed  Europe PubMed DOI
  27. Sripa,J., Pinlaor,P., Brindley,P.J., Sripa,B., Kaewkes,S., Robinson,M.W., Young,N.D., Gasser,R.B., Loukas,A. and Laha,T.
    RNA interference targeting cathepsin B of the carcinogenic liver fluke, Opisthorchis viverrini
    Parasitol Int (2011) 60, 283-288. PubMed  Europe PubMed DOI  K
    28. 2010
  28. Donnelly,S., O'Neill,S.M., Stack,C.M., Robinson,M.W., Turnbull,L., Whitchurch,C. and Dalton,J.P.
    Helminth cysteine proteases inhibit TRIF-dependent activation of macrophages via degradation of TLR3
    J Biol Chem (2010) 285, 3383-3392. PubMed  Europe PubMed DOI
  29. Sripa,J., Laha,T., To,J., Brindley,P.J., Sripa,B., Kaewkes,S., Dalton,J.P. and Robinson,M.W.
    Secreted cysteine proteases of the carcinogenic liver fluke, Opisthorchis viverrini: regulation of cathepsin F activation by autocatalysis and trans-processing by cathepsin B
    Cell Microbiol (2010) 12, 781-795. PubMed  Europe PubMed DOI
  30. Tchoubrieva,E.B., Ong,P.C., Pike,R.N., Brindley,P.J. and Kalinna,B.H.
    Vector-based RNA interference of cathepsin B1 in Schistosoma mansoni
    Cell Mol Life Sci (2010) 67, 3739-3748. PubMed  Europe PubMed DOI  K
    31. 2009
  31. Doleckova,K., Kasny,M., Mikes,L., Cartwright,J., Jedelsky,P., Schneider,E.L., Dvorak,J., Mountford,A.P., Craik,C.S. and Horak,P.
    The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti
    Int J Parasitol (2009) 39, 201-211. PubMed  Europe PubMed DOI  PMC  EPMC
    32. 2008
  32. Kaewpitoon,N., Laha,T., Kaewkes,S., Yongvanit,P., Brindley,P.J., Loukas,A. and Sripa,B.
    Characterization of cysteine proteases from the carcinogenic liver fluke, Opisthorchis viverrini
    Parasitol Res (2008) 102, 757-764. PubMed  Europe PubMed DOI
  33. Krautz-Peterson,G. and Skelly,P.J.
    Schistosome asparaginyl endopeptidase (legumain) is not essential for cathepsin B1 activation in vivo
    Mol Biochem Parasitol (2008) 159, 54-58. PubMed  Europe PubMed DOI  PMC  EPMC
    34. 2006
  34. [YEAR:6-3-2006]Choe,Y., Leonetti,F., Greenbaum,D.C., Lecaille,F., Bogyo,M., Bromme,D., Ellman,J.A. and Craik,C.S.
    Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities
    J Biol Chem (6-3-2006) 281, 12824-12832. PubMed  Europe PubMed DOI  P
    35. 2005
  35. [YEAR:30-7-2005]Correnti,J.M., Brindley,P.J. and Pearce,E.J.
    Long-term suppression of cathepsin B levels by RNA interference retards schistosome growth
    Mol Biochem Parasitol (30-7-2005) 143, 209-215. PubMed  Europe PubMed DOI
  36. [YEAR:8-6-2005]Dvorak,J., Delcroix,M., Rossi,A., Vopalensky,V., Pospisek,M., Sedinova,M., Mikes,L., Sajid,M., Sali,A., McKerrow,J.H., Horak,P. and Caffrey,C.R.
    Multiple cathepsin B isoforms in schistosomula of Trichobilharzia regenti: identification, characterisation and putative role in migration and nutrition
    Int J Parasitol (8-6-2005) 35, 895-910. PubMed  Europe PubMed DOI
  37. Stack,C.M., Dalton,J.P., Cunneen,M. and Donnelly,S.
    De-glycosylation of Pichia pastoris-produced Schistosoma mansoni cathepsin B eliminates non-specific reactivity with IgG in normal human serum
    J Immunol Methods (2005) 304, 151-157. PubMed  Europe PubMed DOI
    38. 2004
  38. Dalton,J.P., McKerrow,J.H. and Brindley,P.J.
    Trematode cysteine endopeptidases
    [ISSN:0-12-079610-4] (2004) 2, 1176-1182.  V
    39. 2003
  39. Sajid,M., McKerrow,J.H., Hansell,E., Mathieu,M.A., Lucas,K.D., Hsieh,I., Greenbaum,D., Bogyo,M., Salter,J.P., Lim,K.C., Franklin,C., Kim,J.H. and Caffrey,C.R.
    Functional expression and characterization of Schistosoma mansoni cathepsin B and its trans-activation by an endogenous asparaginyl endopeptidase
    Mol Biochem Parasitol (2003) 131, 65-75. PubMed  Europe PubMed DOI
    40. 2002
  40. Sajid,M. and McKerrow,J.H.
    Cysteine proteases of parasitic organisms
    Mol Biochem Parasitol (2002) 120, 1-21. PubMed  Europe PubMed
    41. 2001
  41. Noya,O., De Noya,B.A., Ballen,D.E., Bermudez,H., Bout,D. and Hoebeke,J.
    Immunogenicity of synthetic peptides from the Sm31 antigen (cathepsin B) of the Schistosoma mansoni adult worms
    Parasite Immunol (2001) 23, 567-573. PubMed  Europe PubMed DOI
  42. Skelly,P.J. and Shoemaker,C.B.
    Schistosoma mansoni proteases Sm31 (cathepsin B) and Sm32 (legumain) are expressed in the cecum and protonephridia of cercariae
    J Parasitol (2001) 87, 1218-1221. PubMed  Europe PubMed DOI
    43. 1998
  43. Choi,M.H., Chai,J.Y. and Lee,S.H.
    Purification and characterization of a 16-kDa cysteine proteinase of Gymnophalloides seoi (Gymnophallidae) metacercariae
    J Parasitol (1998) 84, 350-355. PubMed  Europe PubMed DOI
  44. El-Sayed,L.H., Ghoneim,H., Demian,S.R., El-Sayed,M.H., Tawfik,N.M., Sakr,I., Abou-Basha,L.M., Renganathan,E., Klinkert,M.Q. and Abou-Rawash,N.
    Diagnostic significance of Schistosoma mansoni proteins Sm31 and Sm32 in human schistosomiasis in an endemic area in Egypt
    Trop Med Int Health (1998) 3, 721-727. PubMed  Europe PubMed DOI
    45. 1997
  45. Caffrey,C.R., Rheinberg,C.E., Mone,H., Jourdane,J., Li,Y.L. and Ruppel,A.
    Schistosoma japonicum, S. mansoni, S. haematobium, S. intercalatum, and S. rodhaini: cysteine-class cathepsin activities in the vomitus of adult worms
    Parasitol Res (1997) 83, 37-41. PubMed  Europe PubMed
  46. Caffrey,C.R. and Ruppel,A.
    Cathepsin B-like activity predominates over cathepsin L-like activity in adult Schistosoma mansoni and S. japonicum
    Parasitol Res (1997) 83, 632-635. PubMed  Europe PubMed DOI
  47. Dalton,J.P., Clough,K.A., Jones,M.K. and Brindley,P.J.
    The cysteine proteinases of Schistosoma mansoni cercariae
    Parasitology (1997) 114, 105-112. PubMed  Europe PubMed DOI
  48. Grogan,J., Rotmans,P., Ghoneim,H., Deelder,A., Yazdanbakhsh,M. and Klinkert,M.Q.
    Recognition of Schistosoma mansoni cathepsins B and L by human IgG1 and IgG4 antibodies
    Parasite Immunol (1997) 19, 215-220. PubMed  Europe PubMed
    49. 1996
  49. Dalton,J.P., Clough,K.A., Jones,M.K. and Brindley,P.J.
    Characterization of the cathepsin-like cysteine proteinases of Schistosoma mansoni
    Infect Immun (1996) 64, 1328-1334. PubMed  Europe PubMed  PMC  EPMC
  50. Li,Y.L., Idris,M.A., Corachan,M., Han,J.J., Kirschfink,M. and Ruppel,A.
    Circulating antigens in schistosomiasis: detection of 31/32-kDa proteins in sera from patients infected with Schistosoma japonicum, S. mansoni, S. haematobium, or S. intercalatum
    Parasitol Res (1996) 82, 14-18. PubMed  Europe PubMed DOI
  51. Lipps,G., Fullkrug,R. and Beck,E.
    Cathepsin B of Schistosoma mansoni. Purification and activation of the recombinant proenzyme secreted by Saccharomyces cerevisiae
    J Biol Chem (1996) 271, 1717-1725. PubMed  Europe PubMed
    52. 1995
  52. Ghoneim,H. and Klinkert,M.Q.
    Biochemical properties of purified cathepsin B from Schistosoma mansoni
    Int J Parasitol (1995) 25, 1515-1519. PubMed  Europe PubMed DOI
    53. 1993
  53. Yamasaki,H. and Aoki,T.
    Cloning and sequence analysis of the major cysteine protease expressed in the trematode parasite Fasciola sp
    Biochem Mol Biol Int (1993) 31, 537-542. PubMed  Europe PubMed
  54. Yoshino,T.P., Lodes,M.J., Rege,A.A. and Chappell,C.L.
    Proteinase activity in miracidia, transformation excretory-secretory products, and primary sporocysts of Schistosoma mansoni
    J Parasitol (1993) 79, 23-31. PubMed  Europe PubMed
    55. 1992
  55. Gotz,B., Felleisen,R., Shaw,E. and Klinkert,M.Q.
    Expression of an active cathepsin B-like protein Sm31 from Schistosoma mansoni in insect cells
    Trop Med Parasitol (1992) 43, 282-284. PubMed  Europe PubMed
    56. 1989
  56. Klinkert,M.Q., Felleisen,R., Link,G., Ruppel,A. and Beck,E.
    Primary structures of Sm31/32 diagnostic proteins of Schistosoma mansoni and their identification as proteases
    Mol Biochem Parasitol (1989) 33, 113-122. PubMed  Europe PubMed
    57. 1988
  57. Zerda,K.S., Dresden,M.H. and Chappell,C.L.
    Schistosoma mansoni: expression and role of cysteine proteinases in developing schistosomula
    Exp Parasitol (1988) 67, 238-246. PubMed  Europe PubMed
    58. 1987
  58. Ruppel,A., Shi,Y.E., Wei,D.X. and Diesfeld,H.J.
    Sera of Schistosoma japonicum-infected patients cross-react with diagnostic 31/32 kD proteins of S. mansoni
    Clin Exp Immunol (1987) 69, 291-298. PubMed  Europe PubMed  PMC  EPMC
    59. 1986
  59. Sung,C.K. and Dresden,M.H.
    Cysteinyl proteinases of Schistosoma mansoni eggs: purification and partial characterization
    J Parasitol (1986) 72, 891-900. PubMed  Europe PubMed
    60. 1979
  60. Asch,H.L. and Dresden,M.H.
    Acidic thiol proteinase activity of Schistosoma mansoni egg extracts
    J Parasitol (1979) 65, 543-549. PubMed  Europe PubMed

feedback
Page created 8-September-2023

EMBL-EBI logo

© 2026 EBI
Terms of use