$VAR1 = undef;

Summary for peptidase M10.003: matrix metallopeptidase-2

Names
MEROPS Name	matrix metallopeptidase-2
Other names	3/4 collagenase, 72 kDa gelatinase, matrix metalloproteinase 2, gelatinase A, MMP-2, MMP2, matrix metalloproteinase 5, tissue gelatinase, type IV collagenase

Domain architecture

MEROPS Classification
Classification	Clan MA >> Subclan MA(M) >> Family M10 >> Subfamily A >> M10.003
Holotype	matrix metallopeptidase-2 (Homo sapiens), Uniprot accession P08253 (peptidase unit: 108-461), MERNUM MER0001080
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Metallo
Peplist	Included in the Peplist with identifier PL00156
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.24
Enzymology	BRENDA database
Proteolytic events	CutDB database (116 cleavages)
Activity status	human: active (Murphy, 2004) mouse: active (by similarity) (Reponen et al., 1992)
Physiology	Role in turnover of extracellular matrix proteins.
Knockout	Mice lacking gelatinase A and also membrane-type matrix metalloproteinase 1 die immediately after birth (Oh et al., 2004). In humans, mutations of gelatinase A can lead to Al-Aqeel Sewairi syndrome, an autosomal recessive disorder with multicentric osteolysis, nodulosis and arthropathy (Al Aqeel, 2005).
Pharmaceutical relevance	Drug target for prevention of pathological tissue damage. Many reports implicate the enzyme in malignant invasion. A mutation causes a multicentric osteolysis and arthritis syndrome (Martignetti et al., 2001).
Pathways	KEGG	Bladder cancer
	KEGG	Estrogen signaling pathway
	KEGG	GnRH signaling pathway
	KEGG	Leukocyte transendothelial migration
	KEGG	Pathways in cancer
	KEGG	Proteoglycans in cancer
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/MMP2
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/p/-/-li/-/-/- (based on 3423 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	252	121	430	482	60	125	590	290
Pro	197	769	70	210	8	15	29	237
Ala	253	373	644	510	111	235	538	332
Val	199	447	130	45	283	445	274	272
Leu	262	365	178	81	1389	390	112	297
Ile	159	288	70	33	557	351	81	168
Met	82	100	44	67	150	97	47	59
Phe	128	81	48	58	177	143	100	126
Tyr	97	51	48	54	131	91	61	53
Trp	11	16	9	39	44	42	25	23
Ser	178	116	483	333	80	230	491	239
Thr	145	93	86	112	61	253	221	162
Cys	37	42	51	39	70	24	11	40
Asn	88	67	130	376	35	120	167	139
Gln	167	79	229	153	129	220	128	149
Asp	148	12	87	206	9	25	161	278
Glu	169	42	161	266	33	123	147	241
Lys	169	54	180	133	66	309	171	191
Arg	139	26	192	106	13	102	27	38
His	92	80	52	120	14	81	42	76