Summary for peptidase M04.001: thermolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Namethermolysin
Other namesgentlyase, neutral proteinase (Bacillus thermoproteolyticus), megateriopeptidase (Bacillus megaterium), NprS g.p. (Geobacillus stearothermophilus), nprT g.p. (Bacillus thermoproteolyticus), Mername-AA133 peptidase, nprM g.p. (Bacillus megaterium)
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(E) >> Family M4 >> Subfamily (none) >> M04.001
Holotypethermolysin (Bacillus thermoproteolyticus), Uniprot accession P00800 (peptidase unit: 233-548), MERNUM MER0001026
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00143
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.27
EnzymologyBRENDA database
Proteolytic eventsCutDB database (16 cleavages)
PhysiologyBacterial nutrition.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/-Scissile bondlivf/-/-/- (based on 1739 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 135 128 101 152 25 144 183 151
Pro 73 69 75 62 5 6 157 200
Ala 125 133 156 140 115 154 104 84
Val 134 144 124 28 314 159 66 53
Leu 148 174 106 115 548 124 71 47
Ile 99 99 75 9 336 104 57 41
Met 36 38 34 42 58 27 25 17
Phe 62 73 76 74 189 45 35 14
Tyr 57 52 62 72 44 43 37 31
Trp 15 19 21 37 5 31 20 11
Ser 90 84 102 129 42 139 117 115
Thr 88 103 75 141 34 137 87 103
Cys 30 24 20 32 0 2 7 4
Asn 51 57 59 88 5 51 60 76
Gln 57 60 71 78 4 58 65 70
Asp 115 85 65 114 5 148 180 236
Glu 129 110 149 133 6 121 199 274
Lys 143 142 181 160 2 114 122 99
Arg 79 77 130 86 0 97 80 54
His 36 38 36 47 2 24 35 23
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Bacillus thermoproteolyticus recombinant - F/V/A K/Y F/broad L G/R K - xFKF+LGKx D-A2pr (Nma) - Dnp Oda et al., 2005
Bacillus thermoproteolyticus recombinant - - - K - - - - xxxK+xxxx ACC-Dabcyl Sun et al., 2007
Relevant inhibitors galardin, phosphoramidon, talopeptin