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Literature for peptidase M04.001: thermolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

(Topics flags: A Assay, S Structure, P Specificity, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2024
  1. Canizares-Carmenate,Y., Perera-Sardina,Y., Marrero-Ponce,Y., Diaz-Amador,R., Torrens,F. and Castillo-Garit,J.A.
    Ligand and structure-based discovery of phosphorus-containing compounds as potential metalloproteinase inhibitors
    SAR QSAR Environ Res35, 219-240. PubMed  Europe PubMed DOI  I
  2. Jiang,X., Yeung,D., Liu,Y., Spicer,V., Afshari,H., Lao,Y., Lin,F., Krokhin,O. and Zahedi,R.P.
    Accelerating Proteomics Using Broad Specificity Proteases
    J Proteome Res23, 1360-1369. PubMed  Europe PubMed DOI
    3. 2023
  3. Berdyshev,I.M., Svetlova,A.O., Chukhontseva,K.N., Karaseva,M.A., Varizhuk,A.M., Filatov,V.V., Kleymenov,S.Y., Kostrov,S.V. and Demidyuk,I.V.
    Production and Characterization of Photorin, a Novel Proteinaceous Protease Inhibitor from the Entomopathogenic Bacteria Photorhabdus laumondii
    Biochemistry (Mosc)88, 1356-1367. PubMed  Europe PubMed DOI
  4. Peng,S., Li,H., Zhang,S., Zhang,R., Cheng,X. and Li,K.
    Isolation of a novel feather-degrading Ectobacillus sp. JY-23 strain and characterization of a new keratinase in the M4 metalloprotease family
    Microbiol Res274, 127439-127439. PubMed  Europe PubMed DOI
    5. 2021
  5. Dong,M.
    A Minireview on Temperature Dependent Protein Conformational Sampling
    Protein J PubMed  Europe PubMed DOI  V
    6. 2020
  6. Dong,M., Lauro,M.L., Koblish,T.J. and Bahnson,B.J.
    Conformational sampling and kinetics changes across a non-Arrhenius break point in the enzyme thermolysin
    Struct Dyn7, 014101-014101. PubMed  Europe PubMed DOI
  7. Fiebig,D., Anderl,A., Al Djaizani,S., Kolmar,H. and Fuchsbauer,H.L.
    Dissecting capture and twisting of aureolysin and pseudolysin: functional amino acids of the Dispase autolysis-inducing protein
    Biochem J477, 2595-2606. PubMed  Europe PubMed DOI
  8. Hernandez-Corroto,E., Sanchez-Milla,M., Sanchez-Nieves,J., de la Mata,F.J., Marina,M.L. and Garcia,M.C.
    Immobilization of thermolysin enzyme on dendronized silica supports. Evaluation of its feasibility on multiple protein hydrolysis cycles
    Int J Biol Macromol165, 2338-2348. PubMed  Europe PubMed DOI
  9. Nam,K.H.
    Structural analysis of metal chelation of the metalloproteinase thermolysin by 1,10-phenanthroline
    J Inorg Biochem215, 111319-111319. PubMed  Europe PubMed DOI  I
    10. 2019
  10. Eisenhardt,M., Schlupp,P., Hofer,F., Schmidts,T., Hoffmann,D., Czermak,P., Poppel,A.K., Vilcinskas,A. and Runkel,F.
    The therapeutic potential of the insect metalloproteinase inhibitor against infections caused by Pseudomonas aeruginosa
    J Pharm Pharmacol71, 316-328. PubMed  Europe PubMed DOI
  11. Kovalchuk,M.V., Boikova,A.S., Dyakova,Y.A., Ilina,K.B., Konarev,P.V., Kryukova,A.E., Marchenkova,M.A., Pisarevsky,Y.V. and Timofeev,V.I.
    Pre-crystallization phase formation of thermolysin hexamers in solution close to crystallization conditions
    J Biomol Struct Dyn37, 3058-3064. PubMed  Europe PubMed DOI
  12. Leite,J.P. and Gales,L.
    Alzheimer's Abeta1-40 peptide degradation by thermolysin: evidence of inhibition by a C-terminal Abeta product
    FEBS Lett593, 128-137. PubMed  Europe PubMed DOI
    13. 2018
  13. Contesini,F.J., Melo,R.R. and Sato,H.H.
    An overview of Bacillus proteases: from production to application
    Crit Rev Biotechnol38, 321-334. PubMed  Europe PubMed DOI
    14. 2017
  14. Cramer,J., Krimmer,S.G., Fridh,V., Wulsdorf,T., Karlsson,R., Heine,A. and Klebe,G.
    Elucidating the origin of long residence time binding for inhibitors of the metalloprotease thermolysin
    ACS Chem Biol12, 225-233. PubMed  Europe PubMed DOI  I
  15. Cramer,J., Krimmer,S.G., Heine,A. and Klebe,G.
    Paying the price of desolvation in solvent-exposed protein pockets: impact of distal solubilizing groups on affinity and binding thermodynamics in a series of thermolysin inhibitors
    J Med Chem60, 5791-5799. PubMed  Europe PubMed DOI  I
  16. Hang,F., Wang,Q., Hong,Q., Gao,C., Zhang,H. and Chen,W.
    Structural insight into a novel neutral metalloproteinase from Paenibacillus spp. BD3526: implications for mechanisms of rapid inactivation and calcium-dependent stability
    Int J Biol Macromol95, 1082-1090. PubMed  Europe PubMed DOI
  17. Krimmer,S.G., Cramer,J., Schiebel,J., Heine,A. and Klebe,G.
    How nothing boosts affinity: hydrophobic ligand binding to the virtually vacated S1' pocket of thermolysin
    J Am Chem Soc139, 10419-10431. PubMed  Europe PubMed DOI
  18. Naitow,H., Matsuura,Y., Tono,K., Joti,Y., Kameshima,T., Hatsui,T., Yabashi,M., Tanaka,R., Tanaka,T., Sugahara,M., Kobayashi,J., Nango,E., Iwata,S. and Kunishima,N.
    Protein-ligand complex structure from serial femtosecond crystallography using soaked thermolysin microcrystals and comparison with structures from synchrotron radiation
    Acta Crystallogr D Struct Biol73, 702-709. PubMed  Europe PubMed DOI  S
    19. 2016
  19. Betz,M., Wulsdorf,T., Krimmer,S.G. and Klebe,G.
    Impact of surface water layers on protein-ligand binding: how well are experimental data reproduced by molecular dynamics simulations in a thermolysin test case?
    J Chem Inf Model56, 223-233. PubMed  Europe PubMed DOI  I
  20. Hang,F., Wang,Q., Hong,Q., Liu,P., Wu,Z., Liu,Z., Zhang,H. and Chen,W.
    Purification and characterization of a novel milk-clotting metalloproteinase from Paenibacillus spp. BD3526
    Int J Biol Macromol85, 547-554. PubMed  Europe PubMed DOI
  21. Krimmer,S.G., Cramer,J., Betz,M., Fridh,V., Karlsson,R., Heine,A. and Klebe,G.
    Rational design of thermodynamic and kinetic binding profiles by optimizing surface water networks coating protein-bound ligands
    J Med Chem59, 10530-10548. PubMed  Europe PubMed DOI  I
  22. Sjoli,S., Nuti,E., Camodeca,C., Bilto,I., Rossello,A., Winberg,J.O., Sylte,I. and Adekoya,O.A.
    Synthesis, experimental evaluation and molecular modelling of hydroxamate derivatives as zinc metalloproteinase inhibitors
    Eur J Med Chem108, 141-153. PubMed  Europe PubMed DOI  I
  23. Zhu,F., Zhuang,Y., Wu,B., Li,J. and He,B.
    Rational substitution of surface acidic residues for enhancing the thermostability of thermolysin
    Appl Biochem Biotechnol178, 725-738. PubMed  Europe PubMed DOI
    24. 2015
  24. Adekoya,O.A., Sjoli,S., Wuxiuer,Y., Bilto,I., Marques,S.M., Santos,M.A., Nuti,E., Cercignani,G., Rossello,A., Winberg,J.-O. and Sylte,I.
    Inhibition of pseudolysin and thermolysin by hydroxamate-based MMP inhibitors
    Eur J Med Chem89, 340-348. PubMed  Europe PubMed DOI  I
  25. Kong,L., Lu,A., Guan,J., Yang,B., Li,M., Hillyer,J.F., Ramarao,N., Soderhall,K., Liu,C. and Ling,E.
    Thermolysin damages animal life through degradation of plasma proteins enhanced by rapid cleavage of serpins and activation of proteases
    Arch Insect Biochem Physiol88, 64-84. PubMed  Europe PubMed DOI
  26. Nasief,N.N. and Hangauer,D.
    Additivity or cooperativity: which model can predict the influence of simultaneous incorporation of two or more functionalities in a ligand molecule?
    Eur J Med Chem90, 897-915. PubMed  Europe PubMed DOI  I
    27. 2014
  27. Birrane,G., Bhyravbhatla,B. and Navia,M.A.
    Synthesis of aspartame by thermolysin: an X-ray structural study
    ACS Med Chem Lett5, 706-710. PubMed  Europe PubMed DOI
  28. Kasper,J.R., Andrews,E.C. and Park,C.
    Product inhibition in native-state proteolysis
    PLoS ONE9, e111416-e111416. PubMed  Europe PubMed DOI
  29. Kim,C.-J., Lee,D.-I., Lee,C.-H. and Ahn,I.-S.
    Dityrosine-based substrates for the selective and sensitive assay of thermolysin
    J Ind Eng Chem21, 248-253. DOI  A
  30. Kojima,K., Nakata,H. and Inouye,K.
    Involvement of Val 315 located in the C-terminal region of thermolysin in its expression in Escherichia coli and its thermal stability
    Biochim Biophys Acta1844, 330-338. PubMed  Europe PubMed DOI  E
  31. Krimmer,S.G., Betz,M., Heine,A. and Klebe,G.
    Methyl, ethyl, propyl, butyl: futile but not for water, as the correlation of structure and thermodynamic signature shows in a congeneric series of thermolysin inhibitors
    ChemMedChem9, 833-846. PubMed  Europe PubMed DOI  I
  32. Samukange,V., Kamo,M., Yasukawa,K. and Inouye,K.
    Effects of salts on the interaction of 8-anilinonaphthalene 1-sulphonate and thermolysin
    Biosci Biotechnol Biochem78, 1522-1528. PubMed  Europe PubMed DOI
    33. 2013
  33. Furukawa,S., Hasegawa,K., Fuke,I., Kittaka,K., Nakakoba,T., Goto,M. and Kamiya,N.
    Enzymatic synthesis of Z-aspartame in liquefied amino acid substrates
    Biochem Eng J70, 84-87. DOI
  34. Kawasaki,Y., Yasukawa,K. and Inouye,K.
    Effects of site-directed mutagenesis in the N-terminal domain of thermolysin on its stabilization
    J Biochem153, 85-92. PubMed  Europe PubMed DOI
  35. Ke,Q., Chen,A., Minoda,M. and Yoshida,H.
    Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus
    Food Chem Toxicol59, 541-548. PubMed  Europe PubMed DOI
  36. Menach,E., Hashida,Y., Yasukawa,K. and Inouye,K.
    Effects of conversion of the zinc-binding motif sequence of thermolysin, HEXXH, to that of dipeptidyl peptidase III, HEXXXH, on the activity and stability of thermolysin
    Biosci Biotechnol Biochem77, 1901-1906. PubMed  Europe PubMed DOI
  37. Menach,E., Yasukawa,K. and Inouye,K.
    Effects of mutations of thermolysin, Asn116 to Asp and Asp150 to Glu, on salt-induced activation and stabilization
    Biosci Biotechnol Biochem77, 741-746. PubMed  Europe PubMed DOI
  38. Ruf,A., Stihle,M., Benz,J., Schmidt,M. and Sobek,H.
    Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa
    Acta Crystallogr D Biol Crystallogr69, 24-31. PubMed  Europe PubMed DOI  S
    39. 2012
  39. Balamurugan,A.N., Loganathan,G., Bellin,M.D., Wilhelm,J.J., Harmon,J., Anazawa,T., Soltani,S.M., Radosevich,D.M., Yuasa,T., Tiwari,M., Papas,K.K., McCarthy,R., Sutherland,D.E. and Hering,B.J.
    A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products
    Transplantation93, 693-702. PubMed  Europe PubMed DOI
  40. Biela,A., Betz,M., Heine,A. and Klebe,G.
    Water makes the difference: rearrangement of water solvation layer triggers non-additivity of functional group contributions in protein-ligand binding
    ChemMedChem7, 1423-1434. PubMed  Europe PubMed DOI  I
  41. Ceruso,M., Howe,N. and Malthouse,J.P.
    Mechanism of the binding of Z-L-tryptophan and Z-L-phenylalanine to thermolysin and stromelysin-1 in aqueous solutions
    Biochim Biophys Acta1824, 303-310. PubMed  Europe PubMed DOI
  42. Chen,F., Zhang,F., Du,F., Wang,A., Gao,W., Wang,Q., Yin,X. and Xie,T.
    A novel and efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation
    Bioresour Technol115, 158-163. PubMed  Europe PubMed DOI
  43. David,A.E., Gong,J., Chertok,B., Domszy,R.C., Moon,C., Park,Y.S., Wang,N.S., Yang,A.J. and Yang,V.C.
    Immobilized thermolysin for highly efficient production of low-molecular-weight protamine - an attractive cell-penetrating peptide for macromolecular drug delivery applications
    J Biomed Mater Res A100, 211-219. PubMed  Europe PubMed DOI
  44. Hirakawa,T., Fujita,S., Ohyama,T., Dedachi,K., Khan,M.T., Sylte,I. and Kurita,N.
    Specific interactions and binding energies between thermolysin and potent inhibitors: molecular simulations based on ab initio molecular orbital method
    J Mol Graph Model33, 1-11. PubMed  Europe PubMed DOI  I
  45. Khan,M.T.H., Wuxiuer,Y. and Sylte,I.
    Binding modes and pharmacophore modelling of thermolysin inhibitors
    Mini Rev Med Chem12, 515-533. PubMed  Europe PubMed  I
  46. Lousa,D., Baptista,A.M. and Soares,C.M.
    Analyzing the molecular basis of enzyme stability in ethanol/water mixtures using molecular dynamics simulations
    J Chem Inf Model52, 465-473. PubMed  Europe PubMed DOI
  47. Menach,E., Yasukawa,K. and Inouye,K.
    Effects of site-directed mutagenesis of Asn116 in the beta-hairpin of the N-terminal domain of thermolysin on its activity and stability
    J Biochem152, 231-239. PubMed  Europe PubMed DOI
  48. Nasief,N.N., Tan,H., Kong,J. and Hangauer,D.
    Water mediated ligand functional group cooperativity: the contribution of a methyl group to binding affinity is enhanced by a COO(-) group through changes in the structure and thermodynamics of the hydration waters of ligand-thermolysin complexes
    J Med Chem55, 8283-8302. PubMed  Europe PubMed DOI  I
  49. Van Den Burg,B. and Eijsink,V.
    Thermolysin and related Bacillus metallopeptidases
    [ISSN:978-0-12-407744-7]3, 540-553. DOI
    50. 2011
  50. Antunez,K., Arredondo,D., Anido,M. and Zunino,P.
    Metalloprotease production by Paenibacillus larvae during the infection of honeybee larvae
    Microbiology (Reading)157, 1474-1480. PubMed  Europe PubMed DOI
  51. Arolas,J.L., Botelho,T.O., Vilcinskas,A. and Gomis-Ruth,F.X.
    Structural evidence for standard-mechanism inhibition in metallopeptidases from a complex poised to resynthesize a peptide bond
    Angew Chem Int Ed Engl50, 10357-10360. PubMed  Europe PubMed DOI  S
  52. Dedachi,K., Hirakawa,T., Fujita,S., Khan,M.T., Sylte,I. and Kurita,N.
    Specific interactions and binding free energies between thermolysin and dipeptides: Molecular simulations combined with Ab initio molecular orbital and classical vibrational analysis
    J Comput Chem32, 3047-3057. PubMed  Europe PubMed DOI
  53. Ghale,G., Ramalingam,V., Urbach,A.R. and Nau,W.M.
    Determining protease substrate selectivity and inhibition by label-free supramolecular tandem enzyme assays
    J Am Chem Soc133, 7528-7535. PubMed  Europe PubMed DOI  A
  54. Tian,G.R., Wang,S.H., Wang,S.F., Meng,L.Q., Li,H., Zeng,Z.H. and Jin,J.Y.
    Nitro-based inhibitors against thermolysin
    MedChemComm2, 698-700. DOI  I
    55. 2010
  55. Englert,L., Biela,A., Zayed,M., Heine,A., Hangauer,D. and Klebe,G.
    Displacement of disordered water molecules from hydrophobic pocket creates enthalpic signature: binding of phosphonamidate to the S1'-pocket of thermolysin
    Biochim Biophys Acta1800, 1192-1202. PubMed  Europe PubMed DOI  S  I
  56. Guilbaud,J.B., Vey,E., Boothroyd,S., Smith,A.M., Ulijn,R.V., Saiani,A. and Miller,A.F.
    Enzymatic catalyzed synthesis and triggered gelation of ionic peptides
    Langmuir26, 11297-11303. PubMed  Europe PubMed DOI
  57. Khan,M.T., Khan,R., Wuxiuer,Y., Arfan,M., Ahmed,M. and Sylte,I.
    Identification of novel quinazolin-4(3H)-ones as inhibitors of thermolysin, the prototype of the M4 family of proteinases
    Bioorg Med Chem18, 4317-4327. PubMed  Europe PubMed DOI  I
  58. Kusano,M., Yasukawa,K. and Inouye,K.
    Effects of the mutational combinations on the activity and stability of thermolysin
    J Biotechnol147, 7-16. PubMed  Europe PubMed DOI  E
  59. Menach,E., Yasukawa,K. and Inouye,K.
    Effects of site-directed mutagenesis of the loop residue of the N-terminal domain Gly117 of thermolysin on its catalytic activity
    Biosci Biotechnol Biochem74, 2457-2462. PubMed  Europe PubMed DOI
    60. 2009
  60. Khan,M.T., Fuskevag,O.M. and Sylte,I.
    Discovery of potent thermolysin inhibitors using structure based virtual screening and binding assays
    J Med Chem52, 48-61. PubMed  Europe PubMed DOI  I
  61. Kusano,M., Yasukawa,K. and Inouye,K.
    Insights into the catalytic roles of the polypeptide regions in the active site of thermolysin and generation of the thermolysin variants with high activity and stability
    J Biochem145, 103-113. PubMed  Europe PubMed DOI
  62. Marguerre,A.K. and Kramer,R.
    Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin
    Bioorg Med Chem Lett19, 5757-5759. PubMed  Europe PubMed DOI  A
  63. Wu,R., Hu,P., Wang,S., Cao,Z. and Zhang,Y.
    Flexibility of catalytic zinc coordination in thermolysin and HDAC8: a Born-Oppenheimer ab initio QM/MM molecular dynamics study
    J Chem Theory Comput6, 337-337. PubMed  Europe PubMed DOI  S
    64. 2008
  64. Dong,M. and Liu,H.
    Origins of the different metal preferences of Escherichia coli peptide deformylase and Bacillus thermoproteolyticus thermolysin: a comparative quantum mechanical/molecular mechanical study
    J Phys Chem B112, 10280-10290. PubMed  Europe PubMed DOI
  65. Liu,Y.H. and Konermann,L.
    Conformational dynamics of free and catalytically active thermolysin are indistinguishable by hydrogen/deuterium exchange mass spectrometry
    Biochemistry47, 6342-6351. PubMed  Europe PubMed DOI
  66. Takita,T., Aono,T., Sakurama,H., Itoh,T., Wada,T., Minoda,M., Yasukawa,K. and Inouye,K.
    Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability
    Biochim Biophys Acta1784, 481-488. PubMed  Europe PubMed DOI
    67. 2007
  67. Mitchell,J.K., Pitcher,D., McArdle,B.M., Alnefelt,T., Duffy,S., Avery,V. and Quinn,R.J.
    Identifying common metalloprotease inhibitors by protein fold types using Fourier transform mass spectrometry
    Bioorg Med Chem Lett17, 6521-6524. PubMed  Europe PubMed DOI  I
  68. Owen,J.P., Maddison,B.C., Whitelam,G.C. and Gough,K.C.
    Use of thermolysin in the diagnosis of prion diseases
    Mol Biotechnol35, 161-170. PubMed  Europe PubMed DOI
  69. [YEAR:18-9-2007]Yasukawa,K. and Inouye,K.
    Improving the activity and stability of thermolysin by site-directed mutagenesis
    Biochim Biophys Acta1774, 1281-1288.
  70. Yasukawa,K., Kusano,M. and Inouye,K.
    A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli
    Protein Eng Des Sel20, 375-383. PubMed  Europe PubMed DOI  E
    71. 2006
  71. [YEAR:1-5-2006]Weimer,S., Oertel,K. and Fuchsbauer,H.L.
    A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
    Anal Biochem352, 110-119. PubMed  Europe PubMed DOI  A
    72. 2005
  72. Oda,K., Takahashi,T., Takada,K., Tsunemi,M., Ng,K.K., Hiraga,K. and Harada,S.
    Exploring the subsite-structure of vimelysin and thermolysin using FRETS-libraries
    FEBS Lett579, 5013-5018. PubMed  Europe PubMed DOI  P
    73. 2004
  73. [YEAR:30-4-2004]Kim,J. and Sieburth,S.M.
    A silanediol inhibitor of the metalloprotease thermolysin: synthesis and comparison with a phosphinic acid inhibitor
    J Org Chem69, 3008-3014. PubMed  Europe PubMed DOI  I
  74. Matsumiya,Y., Nishikawa,K., Aoshima,H., Inouye,K. and Kubo,M.
    Analysis of autodegradation sites of thermolysin and enhancement of its thermostability by modifying Leu155 at an autodegradation site
    J Biochem135, 547-553. PubMed  Europe PubMed DOI
  75. Van Den Burg,B. and Eijsink,V.
    Thermolysin and related Bacillus metallopeptidases
    [ISSN:0-12-079610-4]2, 374-387.  V
    76. 2003
  76. Selkti,M., Tomas,A., Gaucher,J.F., Prange,T., Fournie-Zaluski,M.C., Chen,H. and Roques,B.P.
    Interactions of a new alpha-aminophosphinic derivative inside the active site of TLN (thermolysin): a model for zinc-metalloendopeptidase inhibition
    Acta Crystallogr D Biol Crystallogr59, 1200-1205. PubMed  Europe PubMed DOI  I
  77. Younus,H., Schops,R., Lerchner,A., Rucknagel,K.P., Schierhorn,A., Saleemuddin,M. and Ulbrich-Hofmann,R.
    Proteolytic sensitivity of a recombinant phospholipase D from cabbage: identification of loop regions and conformational changes
    J Protein Chem22, 499-508. PubMed  Europe PubMed DOI  P
    78. 2002
  78. Gaucher,J.F., Selkti,M., Prange,T. and Tomas,A.
    The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate
    Acta Crystallogr D Biol Crystallogr58, 2198-2200. PubMed  Europe PubMed DOI  S
  79. Hausrath,A.C. and Matthews,B.W.
    Thermolysin in the absence of substrate has an open conformation
    Acta Crystallogr D Biol Crystallogr58, 1002-1007. PubMed  Europe PubMed DOI  S
  80. Kim,J., Glekas,A. and Sieburth,S.
    Silanediol-based inhibitor of thermolysin
    Bioorg Med Chem Lett12, 3625-3627. PubMed  Europe PubMed DOI  I
  81. Muta,Y. and Inouye,K.
    Inhibitory effects of alcohols on thermolysin activity as examined using a fluorescent substrate
    J Biochem132, 945-951. PubMed  Europe PubMed
    82. 2001
  82. de Kreij,A., Van Den Burg,B., Veltman,O.R., Vriend,G., Venema,G. and Eijsink,V.G.H.
    The effect of changing the hydrophobic S1' subsite of thermolysin-like proteases on substrate specificity
    Eur J Biochem268, 4985-4991. PubMed  Europe PubMed DOI
  83. [YEAR:7-5-2001]De Martin,L., Ebert,C., Gardossi,L. and Linda,P.
    High isolated yields in thermolysin-catalysed synthesis of Z-L-aspartyl-L-phenylalanine methyl ester in toluene at controlled water activity
    Tetrahedron Lett42, 3395-3397.
  84. Eijsink,V.G.H., Vriend,G. and Van Den Burg,B.
    Engineering a hyperstable enzyme by manipulation of early steps in the unfolding process
    Biocatal Biotransformation19, 443-458.
  85. Kuzuya,K. and Inouye,K.
    Effects of cobalt-substitution of the active zinc ion in thermolysin on its activity and active-site microenvironment
    J Biochem130, 783-788. PubMed  Europe PubMed  I
  86. [YEAR:5-9-2001]Murakami,Y., Chiba,K., Oda,T. and Hirata,A.
    Novel kinetic analysis of enzymatic dipeptide synthesis: effect of pH and substrates on thermolysin catalysis
    Biotechnol Bioeng74, 406-415. PubMed  Europe PubMed DOI
    87. 2000
  87. [YEAR:6-10-2000]de Kreij,A., Venema,G. and Van Den Burg,B.
    Substrate specificity in the highly heterogeneous M4 peptidase family is determined by a small subset of amino acids
    J Biol Chem275, 31115-31120. PubMed  Europe PubMed DOI
    88. 1999
  88. Baltora-Rosset,S., Aboubeker,A., Dupradeau,F.Y., Pauthe,E., Gacel,G.A., Larreta-Garde,V. and Monti,J.P.
    Structural studies by 1H NMR and molecular modeling of peptide substrates of thermolysin in relation with its proteasic activity in water and glycerol
    J Biomol Struct Dyn16, 1061-1074. PubMed  Europe PubMed DOI
  89. [YEAR:1-12-1999]English,A.C., Done,S.H., Caves,L.S., Groom,C.R. and Hubbard,R.E.
    Locating interaction sites on proteins: The crystal structure of thermolysin soaked in 2% to 100% isopropanol
    Proteins37, 628-640. PubMed  Europe PubMed DOI  S
  90. Makarova,K.S. and Grishin,N.V.
    Thermolysin and mitochondrial processing peptidase: how far structure-functional convergence goes
    Protein Sci8, 2537-2540. PubMed  Europe PubMed DOI
  91. [YEAR:5-2-1999]Marie-Claire,C., Ruffet,E., Beaumont,A. and Roques,B.P.
    The prosequence of thermolysin acts as an intramolecular chaperone when expressed in trans with the mature sequence in Escherichia coli
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  92. Pauthe,E., Dauchez,M., Mejri,M., Berjot,M., Mathlouthi,M., Larreta-Garde,V. and Alix,A.J.P.
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