Literature for peptidase M04.001: thermolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

(Topics flags: A Assay, S Structure, P Specificity, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2024

Canizares-Carmenate,Y., Perera-Sardina,Y., Marrero-Ponce,Y., Diaz-Amador,R., Torrens,F. and Castillo-Garit,J.A. Ligand and structure-based discovery of phosphorus-containing compounds as potential metalloproteinase inhibitors SAR QSAR Environ Res (2024) 35, 219-240. PubMed Europe PubMed DOI I
Jiang,X., Yeung,D., Liu,Y., Spicer,V., Afshari,H., Lao,Y., Lin,F., Krokhin,O. and Zahedi,R.P. Accelerating Proteomics Using Broad Specificity Proteases J Proteome Res (2024) 23, 1360-1369. PubMed Europe PubMed DOI

2023

Berdyshev,I.M., Svetlova,A.O., Chukhontseva,K.N., Karaseva,M.A., Varizhuk,A.M., Filatov,V.V., Kleymenov,S.Y., Kostrov,S.V. and Demidyuk,I.V. Production and Characterization of Photorin, a Novel Proteinaceous Protease Inhibitor from the Entomopathogenic Bacteria Photorhabdus laumondii Biochemistry (Mosc) (2023) 88, 1356-1367. PubMed Europe PubMed DOI
Peng,S., Li,H., Zhang,S., Zhang,R., Cheng,X. and Li,K. Isolation of a novel feather-degrading Ectobacillus sp. JY-23 strain and characterization of a new keratinase in the M4 metalloprotease family Microbiol Res (2023) 274, 127439-127439. PubMed Europe PubMed DOI

2021

Dong,M. A Minireview on Temperature Dependent Protein Conformational Sampling Protein J (2021) PubMed Europe PubMed DOI V

2020

Dong,M., Lauro,M.L., Koblish,T.J. and Bahnson,B.J. Conformational sampling and kinetics changes across a non-Arrhenius break point in the enzyme thermolysin Struct Dyn (2020) 7, 014101-014101. PubMed Europe PubMed DOI PMC EPMC
Fiebig,D., Anderl,A., Al Djaizani,S., Kolmar,H. and Fuchsbauer,H.L. Dissecting capture and twisting of aureolysin and pseudolysin: functional amino acids of the Dispase autolysis-inducing protein Biochem J (2020) 477, 2595-2606. PubMed Europe PubMed DOI
Goulet,D.L., Fraaz,U., Zulich,C.J., Pilkington,T.J. and Siemann,S. Specificity-directed design of a FRET-quenched heptapeptide for assaying thermolysin-like proteases Anal Biochem (2020) 604, 113826-113826. PubMed Europe PubMed DOI
Hernandez-Corroto,E., Sanchez-Milla,M., Sanchez-Nieves,J., de la Mata,F.J., Marina,M.L. and Garcia,M.C. Immobilization of thermolysin enzyme on dendronized silica supports. Evaluation of its feasibility on multiple protein hydrolysis cycles Int J Biol Macromol (2020) 165, 2338-2348. PubMed Europe PubMed DOI
Nam,K.H. Structural analysis of metal chelation of the metalloproteinase thermolysin by 1,10-phenanthroline J Inorg Biochem (2020) 215, 111319-111319. PubMed Europe PubMed DOI I

2019

Eisenhardt,M., Schlupp,P., Hofer,F., Schmidts,T., Hoffmann,D., Czermak,P., Poppel,A.K., Vilcinskas,A. and Runkel,F. The therapeutic potential of the insect metalloproteinase inhibitor against infections caused by Pseudomonas aeruginosa J Pharm Pharmacol (2019) 71, 316-328. PubMed Europe PubMed DOI
Kovalchuk,M.V., Boikova,A.S., Dyakova,Y.A., Ilina,K.B., Konarev,P.V., Kryukova,A.E., Marchenkova,M.A., Pisarevsky,Y.V. and Timofeev,V.I. Pre-crystallization phase formation of thermolysin hexamers in solution close to crystallization conditions J Biomol Struct Dyn (2019) 37, 3058-3064. PubMed Europe PubMed DOI
Leite,J.P. and Gales,L. Alzheimer's Abeta1-40 peptide degradation by thermolysin: evidence of inhibition by a C-terminal Abeta product FEBS Lett (2019) 593, 128-137. PubMed Europe PubMed DOI

2018

Contesini,F.J., Melo,R.R. and Sato,H.H. An overview of Bacillus proteases: from production to application Crit Rev Biotechnol (2018) 38, 321-334. PubMed Europe PubMed DOI

2017

Cramer,J., Krimmer,S.G., Fridh,V., Wulsdorf,T., Karlsson,R., Heine,A. and Klebe,G. Elucidating the origin of long residence time binding for inhibitors of the metalloprotease thermolysin ACS Chem Biol (2017) 12, 225-233. PubMed Europe PubMed DOI I
Cramer,J., Krimmer,S.G., Heine,A. and Klebe,G. Paying the price of desolvation in solvent-exposed protein pockets: impact of distal solubilizing groups on affinity and binding thermodynamics in a series of thermolysin inhibitors J Med Chem (2017) 60, 5791-5799. PubMed Europe PubMed DOI I
Hang,F., Wang,Q., Hong,Q., Gao,C., Zhang,H. and Chen,W. Structural insight into a novel neutral metalloproteinase from Paenibacillus spp. BD3526: implications for mechanisms of rapid inactivation and calcium-dependent stability Int J Biol Macromol (2017) 95, 1082-1090. PubMed Europe PubMed DOI
Krimmer,S.G., Cramer,J., Schiebel,J., Heine,A. and Klebe,G. How nothing boosts affinity: hydrophobic ligand binding to the virtually vacated S1' pocket of thermolysin J Am Chem Soc (2017) 139, 10419-10431. PubMed Europe PubMed DOI
Naitow,H., Matsuura,Y., Tono,K., Joti,Y., Kameshima,T., Hatsui,T., Yabashi,M., Tanaka,R., Tanaka,T., Sugahara,M., Kobayashi,J., Nango,E., Iwata,S. and Kunishima,N. Protein-ligand complex structure from serial femtosecond crystallography using soaked thermolysin microcrystals and comparison with structures from synchrotron radiation Acta Crystallogr D Struct Biol (2017) 73, 702-709. PubMed Europe PubMed DOI PMC EPMC S

2016

Betz,M., Wulsdorf,T., Krimmer,S.G. and Klebe,G. Impact of surface water layers on protein-ligand binding: how well are experimental data reproduced by molecular dynamics simulations in a thermolysin test case? J Chem Inf Model (2016) 56, 223-233. PubMed Europe PubMed DOI I
Hang,F., Wang,Q., Hong,Q., Liu,P., Wu,Z., Liu,Z., Zhang,H. and Chen,W. Purification and characterization of a novel milk-clotting metalloproteinase from Paenibacillus spp. BD3526 Int J Biol Macromol (2016) 85, 547-554. PubMed Europe PubMed DOI
Krimmer,S.G., Cramer,J., Betz,M., Fridh,V., Karlsson,R., Heine,A. and Klebe,G. Rational design of thermodynamic and kinetic binding profiles by optimizing surface water networks coating protein-bound ligands J Med Chem (2016) 59, 10530-10548. PubMed Europe PubMed DOI I
Sjoli,S., Nuti,E., Camodeca,C., Bilto,I., Rossello,A., Winberg,J.O., Sylte,I. and Adekoya,O.A. Synthesis, experimental evaluation and molecular modelling of hydroxamate derivatives as zinc metalloproteinase inhibitors Eur J Med Chem (2016) 108, 141-153. PubMed Europe PubMed DOI I
Zhu,F., Zhuang,Y., Wu,B., Li,J. and He,B. Rational substitution of surface acidic residues for enhancing the thermostability of thermolysin Appl Biochem Biotechnol (2016) 178, 725-738. PubMed Europe PubMed DOI

2015

Adekoya,O.A., Sjoli,S., Wuxiuer,Y., Bilto,I., Marques,S.M., Santos,M.A., Nuti,E., Cercignani,G., Rossello,A., Winberg,J.-O. and Sylte,I. Inhibition of pseudolysin and thermolysin by hydroxamate-based MMP inhibitors Eur J Med Chem (2015) 89, 340-348. PubMed Europe PubMed DOI I
Kong,L., Lu,A., Guan,J., Yang,B., Li,M., Hillyer,J.F., Ramarao,N., Soderhall,K., Liu,C. and Ling,E. Thermolysin damages animal life through degradation of plasma proteins enhanced by rapid cleavage of serpins and activation of proteases Arch Insect Biochem Physiol (2015) 88, 64-84. PubMed Europe PubMed DOI
Nasief,N.N. and Hangauer,D. Additivity or cooperativity: which model can predict the influence of simultaneous incorporation of two or more functionalities in a ligand molecule? Eur J Med Chem (2015) 90, 897-915. PubMed Europe PubMed DOI I

2014

Birrane,G., Bhyravbhatla,B. and Navia,M.A. Synthesis of aspartame by thermolysin: an X-ray structural study ACS Med Chem Lett (2014) 5, 706-710. PubMed Europe PubMed DOI PMC EPMC
Kasper,J.R., Andrews,E.C. and Park,C. Product inhibition in native-state proteolysis PLoS ONE (2014) 9, e111416-e111416. PubMed Europe PubMed DOI PMC EPMC
Kim,C.-J., Lee,D.-I., Lee,C.-H. and Ahn,I.-S. Dityrosine-based substrates for the selective and sensitive assay of thermolysin J Ind Eng Chem (2014) 21, 248-253. DOI A
Kojima,K., Nakata,H. and Inouye,K. Involvement of Val 315 located in the C-terminal region of thermolysin in its expression in Escherichia coli and its thermal stability Biochim Biophys Acta (2014) 1844, 330-338. PubMed Europe PubMed DOI E
Krimmer,S.G., Betz,M., Heine,A. and Klebe,G. Methyl, ethyl, propyl, butyl: futile but not for water, as the correlation of structure and thermodynamic signature shows in a congeneric series of thermolysin inhibitors ChemMedChem (2014) 9, 833-846. PubMed Europe PubMed DOI I
Samukange,V., Kamo,M., Yasukawa,K. and Inouye,K. Effects of salts on the interaction of 8-anilinonaphthalene 1-sulphonate and thermolysin Biosci Biotechnol Biochem (2014) 78, 1522-1528. PubMed Europe PubMed DOI

2013

Furukawa,S., Hasegawa,K., Fuke,I., Kittaka,K., Nakakoba,T., Goto,M. and Kamiya,N. Enzymatic synthesis of Z-aspartame in liquefied amino acid substrates Biochem Eng J (2013) 70, 84-87. DOI
Kawasaki,Y., Yasukawa,K. and Inouye,K. Effects of site-directed mutagenesis in the N-terminal domain of thermolysin on its stabilization J Biochem (2013) 153, 85-92. PubMed Europe PubMed DOI
Ke,Q., Chen,A., Minoda,M. and Yoshida,H. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus Food Chem Toxicol (2013) 59, 541-548. PubMed Europe PubMed DOI
Menach,E., Yasukawa,K. and Inouye,K. Effects of mutations of thermolysin, Asn116 to Asp and Asp150 to Glu, on salt-induced activation and stabilization Biosci Biotechnol Biochem (2013) 77, 741-746. PubMed Europe PubMed DOI
Menach,E., Hashida,Y., Yasukawa,K. and Inouye,K. Effects of conversion of the zinc-binding motif sequence of thermolysin, HEXXH, to that of dipeptidyl peptidase III, HEXXXH, on the activity and stability of thermolysin Biosci Biotechnol Biochem (2013) 77, 1901-1906. PubMed Europe PubMed DOI
Ruf,A., Stihle,M., Benz,J., Schmidt,M. and Sobek,H. Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa Acta Crystallogr D Biol Crystallogr (2013) 69, 24-31. PubMed Europe PubMed DOI PMC EPMC S

2012

Balamurugan,A.N., Loganathan,G., Bellin,M.D., Wilhelm,J.J., Harmon,J., Anazawa,T., Soltani,S.M., Radosevich,D.M., Yuasa,T., Tiwari,M., Papas,K.K., McCarthy,R., Sutherland,D.E. and Hering,B.J. A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products Transplantation (2012) 93, 693-702. PubMed Europe PubMed DOI
Biela,A., Betz,M., Heine,A. and Klebe,G. Water makes the difference: rearrangement of water solvation layer triggers non-additivity of functional group contributions in protein-ligand binding ChemMedChem (2012) 7, 1423-1434. PubMed Europe PubMed DOI I
Ceruso,M., Howe,N. and Malthouse,J.P. Mechanism of the binding of Z-L-tryptophan and Z-L-phenylalanine to thermolysin and stromelysin-1 in aqueous solutions Biochim Biophys Acta (2012) 1824, 303-310. PubMed Europe PubMed DOI
Chen,F., Zhang,F., Du,F., Wang,A., Gao,W., Wang,Q., Yin,X. and Xie,T. A novel and efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation Bioresour Technol (2012) 115, 158-163. PubMed Europe PubMed DOI
David,A.E., Gong,J., Chertok,B., Domszy,R.C., Moon,C., Park,Y.S., Wang,N.S., Yang,A.J. and Yang,V.C. Immobilized thermolysin for highly efficient production of low-molecular-weight protamine - an attractive cell-penetrating peptide for macromolecular drug delivery applications J Biomed Mater Res A (2012) 100, 211-219. PubMed Europe PubMed DOI
Hirakawa,T., Fujita,S., Ohyama,T., Dedachi,K., Khan,M.T., Sylte,I. and Kurita,N. Specific interactions and binding energies between thermolysin and potent inhibitors: molecular simulations based on ab initio molecular orbital method J Mol Graph Model (2012) 33, 1-11. PubMed Europe PubMed DOI I
Khan,M.T.H., Wuxiuer,Y. and Sylte,I. Binding modes and pharmacophore modelling of thermolysin inhibitors Mini Rev Med Chem (2012) 12, 515-533. PubMed Europe PubMed I
Lousa,D., Baptista,A.M. and Soares,C.M. Analyzing the molecular basis of enzyme stability in ethanol/water mixtures using molecular dynamics simulations J Chem Inf Model (2012) 52, 465-473. PubMed Europe PubMed DOI
Menach,E., Yasukawa,K. and Inouye,K. Effects of site-directed mutagenesis of Asn116 in the beta-hairpin of the N-terminal domain of thermolysin on its activity and stability J Biochem (2012) 152, 231-239. PubMed Europe PubMed DOI
Nasief,N.N., Tan,H., Kong,J. and Hangauer,D. Water mediated ligand functional group cooperativity: the contribution of a methyl group to binding affinity is enhanced by a COO(-) group through changes in the structure and thermodynamics of the hydration waters of ligand-thermolysin complexes J Med Chem (2012) 55, 8283-8302. PubMed Europe PubMed DOI I
Van Den Burg,B. and Eijsink,V. Thermolysin and related Bacillus metallopeptidases [ISSN:978-0-12-407744-7] (2012) 3, 540-553. DOI

2011

Antunez,K., Arredondo,D., Anido,M. and Zunino,P. Metalloprotease production by Paenibacillus larvae during the infection of honeybee larvae Microbiology (Reading) (2011) 157, 1474-1480. PubMed Europe PubMed DOI
Arolas,J.L., Botelho,T.O., Vilcinskas,A. and Gomis-Ruth,F.X. Structural evidence for standard-mechanism inhibition in metallopeptidases from a complex poised to resynthesize a peptide bond Angew Chem Int Ed Engl (2011) 50, 10357-10360. PubMed Europe PubMed DOI S
Dedachi,K., Hirakawa,T., Fujita,S., Khan,M.T., Sylte,I. and Kurita,N. Specific interactions and binding free energies between thermolysin and dipeptides: Molecular simulations combined with Ab initio molecular orbital and classical vibrational analysis J Comput Chem (2011) 32, 3047-3057. PubMed Europe PubMed DOI
Ghale,G., Ramalingam,V., Urbach,A.R. and Nau,W.M. Determining protease substrate selectivity and inhibition by label-free supramolecular tandem enzyme assays J Am Chem Soc (2011) 133, 7528-7535. PubMed Europe PubMed DOI A
Tian,G.R., Wang,S.H., Wang,S.F., Meng,L.Q., Li,H., Zeng,Z.H. and Jin,J.Y. Nitro-based inhibitors against thermolysin MedChemComm (2011) 2, 698-700. DOI I

2010

Englert,L., Biela,A., Zayed,M., Heine,A., Hangauer,D. and Klebe,G. Displacement of disordered water molecules from hydrophobic pocket creates enthalpic signature: binding of phosphonamidate to the S1'-pocket of thermolysin Biochim Biophys Acta (2010) 1800, 1192-1202. PubMed Europe PubMed DOI S I
Guilbaud,J.B., Vey,E., Boothroyd,S., Smith,A.M., Ulijn,R.V., Saiani,A. and Miller,A.F. Enzymatic catalyzed synthesis and triggered gelation of ionic peptides Langmuir (2010) 26, 11297-11303. PubMed Europe PubMed DOI
Khan,M.T., Khan,R., Wuxiuer,Y., Arfan,M., Ahmed,M. and Sylte,I. Identification of novel quinazolin-4(3H)-ones as inhibitors of thermolysin, the prototype of the M4 family of proteinases Bioorg Med Chem (2010) 18, 4317-4327. PubMed Europe PubMed DOI I
Kusano,M., Yasukawa,K. and Inouye,K. Effects of the mutational combinations on the activity and stability of thermolysin J Biotechnol (2010) 147, 7-16. PubMed Europe PubMed DOI E
Menach,E., Yasukawa,K. and Inouye,K. Effects of site-directed mutagenesis of the loop residue of the N-terminal domain Gly117 of thermolysin on its catalytic activity Biosci Biotechnol Biochem (2010) 74, 2457-2462. PubMed Europe PubMed DOI

2009

Khan,M.T., Fuskevag,O.M. and Sylte,I. Discovery of potent thermolysin inhibitors using structure based virtual screening and binding assays J Med Chem (2009) 52, 48-61. PubMed Europe PubMed DOI I
Kusano,M., Yasukawa,K. and Inouye,K. Insights into the catalytic roles of the polypeptide regions in the active site of thermolysin and generation of the thermolysin variants with high activity and stability J Biochem (2009) 145, 103-113. PubMed Europe PubMed DOI
Marguerre,A.K. and Kramer,R. Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin Bioorg Med Chem Lett (2009) 19, 5757-5759. PubMed Europe PubMed DOI A
Wu,R., Hu,P., Wang,S., Cao,Z. and Zhang,Y. Flexibility of catalytic zinc coordination in thermolysin and HDAC8: a Born-Oppenheimer ab initio QM/MM molecular dynamics study J Chem Theory Comput (2009) 6, 337-337. PubMed Europe PubMed DOI PMC EPMC S

2008

Dong,M. and Liu,H. Origins of the different metal preferences of Escherichia coli peptide deformylase and Bacillus thermoproteolyticus thermolysin: a comparative quantum mechanical/molecular mechanical study J Phys Chem B (2008) 112, 10280-10290. PubMed Europe PubMed DOI
Liu,Y.H. and Konermann,L. Conformational dynamics of free and catalytically active thermolysin are indistinguishable by hydrogen/deuterium exchange mass spectrometry Biochemistry (2008) 47, 6342-6351. PubMed Europe PubMed DOI
Takita,T., Aono,T., Sakurama,H., Itoh,T., Wada,T., Minoda,M., Yasukawa,K. and Inouye,K. Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability Biochim Biophys Acta (2008) 1784, 481-488. PubMed Europe PubMed DOI

2007

Mitchell,J.K., Pitcher,D., McArdle,B.M., Alnefelt,T., Duffy,S., Avery,V. and Quinn,R.J. Identifying common metalloprotease inhibitors by protein fold types using Fourier transform mass spectrometry Bioorg Med Chem Lett (2007) 17, 6521-6524. PubMed Europe PubMed DOI I
Owen,J.P., Maddison,B.C., Whitelam,G.C. and Gough,K.C. Use of thermolysin in the diagnosis of prion diseases Mol Biotechnol (2007) 35, 161-170. PubMed Europe PubMed DOI
Yasukawa,K., Kusano,M. and Inouye,K. A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli Protein Eng Des Sel (2007) 20, 375-383. PubMed Europe PubMed DOI E
[YEAR:18-9-2007]Yasukawa,K. and Inouye,K. Improving the activity and stability of thermolysin by site-directed mutagenesis Biochim Biophys Acta (18-9-2007) 1774, 1281-1288.

2006

[YEAR:1-5-2006]Weimer,S., Oertel,K. and Fuchsbauer,H.L. A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases Anal Biochem (1-5-2006) 352, 110-119. PubMed Europe PubMed DOI A

2005

Oda,K., Takahashi,T., Takada,K., Tsunemi,M., Ng,K.K., Hiraga,K. and Harada,S. Exploring the subsite-structure of vimelysin and thermolysin using FRETS-libraries FEBS Lett (2005) 579, 5013-5018. PubMed Europe PubMed DOI P

2004

[YEAR:30-4-2004]Kim,J. and Sieburth,S.M. A silanediol inhibitor of the metalloprotease thermolysin: synthesis and comparison with a phosphinic acid inhibitor J Org Chem (30-4-2004) 69, 3008-3014. PubMed Europe PubMed DOI I
Matsumiya,Y., Nishikawa,K., Aoshima,H., Inouye,K. and Kubo,M. Analysis of autodegradation sites of thermolysin and enhancement of its thermostability by modifying Leu155 at an autodegradation site J Biochem (2004) 135, 547-553. PubMed Europe PubMed DOI
Van Den Burg,B. and Eijsink,V. Thermolysin and related Bacillus metallopeptidases [ISSN:0-12-079610-4] (2004) 2, 374-387. V

2003

Selkti,M., Tomas,A., Gaucher,J.F., Prange,T., Fournie-Zaluski,M.C., Chen,H. and Roques,B.P. Interactions of a new alpha-aminophosphinic derivative inside the active site of TLN (thermolysin): a model for zinc-metalloendopeptidase inhibition Acta Crystallogr D Biol Crystallogr (2003) 59, 1200-1205. PubMed Europe PubMed DOI I
Younus,H., Schops,R., Lerchner,A., Rucknagel,K.P., Schierhorn,A., Saleemuddin,M. and Ulbrich-Hofmann,R. Proteolytic sensitivity of a recombinant phospholipase D from cabbage: identification of loop regions and conformational changes J Protein Chem (2003) 22, 499-508. PubMed Europe PubMed DOI P

2002

Gaucher,J.F., Selkti,M., Prange,T. and Tomas,A. The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate Acta Crystallogr D Biol Crystallogr (2002) 58, 2198-2200. PubMed Europe PubMed DOI S
Hausrath,A.C. and Matthews,B.W. Thermolysin in the absence of substrate has an open conformation Acta Crystallogr D Biol Crystallogr (2002) 58, 1002-1007. PubMed Europe PubMed DOI S
Kim,J., Glekas,A. and Sieburth,S. Silanediol-based inhibitor of thermolysin Bioorg Med Chem Lett (2002) 12, 3625-3627. PubMed Europe PubMed DOI I
Muta,Y. and Inouye,K. Inhibitory effects of alcohols on thermolysin activity as examined using a fluorescent substrate J Biochem (2002) 132, 945-951. PubMed Europe PubMed

2001

de Kreij,A., Van Den Burg,B., Veltman,O.R., Vriend,G., Venema,G. and Eijsink,V.G.H. The effect of changing the hydrophobic S1' subsite of thermolysin-like proteases on substrate specificity Eur J Biochem (2001) 268, 4985-4991. PubMed Europe PubMed DOI
[YEAR:7-5-2001]De Martin,L., Ebert,C., Gardossi,L. and Linda,P. High isolated yields in thermolysin-catalysed synthesis of Z-L-aspartyl-L-phenylalanine methyl ester in toluene at controlled water activity Tetrahedron Lett (7-5-2001) 42, 3395-3397.
Eijsink,V.G.H., Vriend,G. and Van Den Burg,B. Engineering a hyperstable enzyme by manipulation of early steps in the unfolding process Biocatal Biotransformation (2001) 19, 443-458.
Kuzuya,K. and Inouye,K. Effects of cobalt-substitution of the active zinc ion in thermolysin on its activity and active-site microenvironment J Biochem (2001) 130, 783-788. PubMed Europe PubMed I
[YEAR:5-9-2001]Murakami,Y., Chiba,K., Oda,T. and Hirata,A. Novel kinetic analysis of enzymatic dipeptide synthesis: effect of pH and substrates on thermolysin catalysis Biotechnol Bioeng (5-9-2001) 74, 406-415. PubMed Europe PubMed DOI

2000

[YEAR:6-10-2000]de Kreij,A., Venema,G. and Van Den Burg,B. Substrate specificity in the highly heterogeneous M4 peptidase family is determined by a small subset of amino acids J Biol Chem (6-10-2000) 275, 31115-31120. PubMed Europe PubMed DOI

1999

Baltora-Rosset,S., Aboubeker,A., Dupradeau,F.Y., Pauthe,E., Gacel,G.A., Larreta-Garde,V. and Monti,J.P. Structural studies by 1H NMR and molecular modeling of peptide substrates of thermolysin in relation with its proteasic activity in water and glycerol J Biomol Struct Dyn (1999) 16, 1061-1074. PubMed Europe PubMed DOI
[YEAR:1-12-1999]English,A.C., Done,S.H., Caves,L.S., Groom,C.R. and Hubbard,R.E. Locating interaction sites on proteins: The crystal structure of thermolysin soaked in 2% to 100% isopropanol Proteins (1-12-1999) 37, 628-640. PubMed Europe PubMed DOI S
Makarova,K.S. and Grishin,N.V. Thermolysin and mitochondrial processing peptidase: how far structure-functional convergence goes Protein Sci (1999) 8, 2537-2540. PubMed Europe PubMed DOI PMC EPMC
[YEAR:5-2-1999]Marie-Claire,C., Ruffet,E., Beaumont,A. and Roques,B.P. The prosequence of thermolysin acts as an intramolecular chaperone when expressed in trans with the mature sequence in Escherichia coli J Mol Biol (5-2-1999) 285, 1911-1915. PubMed Europe PubMed DOI E
Pauthe,E., Dauchez,M., Mejri,M., Berjot,M., Mathlouthi,M., Larreta-Garde,V. and Alix,A.J.P. Structural studies of a small (linear, cyclic) peptide as a synthetic substrate for thermolysin J Mol Struct (1999) 480-481, 423-426. DOI

1998

Inouye,K., Kuzuya,K. and Tonomura,B. Effect of salts on the solubility of thermolysin: A remarkable increase in the solubility as well as the activity by the addition of salts without aggregation or dispersion of thermolysin J Biochem (1998) 123, 847-852. PubMed Europe PubMed
Inouye,K., Mazda,N. and Kubo,M. Need for aromatic residue at position 115 for proteolytic activity found by site-directed mutagenesis of tryptophan 115 in thermolysin Biosci Biotechnol Biochem (1998) 62, 798-800. PubMed Europe PubMed
Inouye,K., Lee,S.B. and Tonomura,B. Effects of nitration and amination of tyrosyl residues in thermolysin on its hydrolytic activity and its remarkable activation by salts J Biochem (1998) 124, 72-78. PubMed Europe PubMed
[YEAR:14-10-1998]Inouye,K., Kuzuya,K. and Tonomura,B. Sodium chloride enhances markedly the thermal stability of thermolysin as well as its catalytic activity Biochim Biophys Acta (14-10-1998) 1388, 209-214. PubMed Europe PubMed DOI
Lee,K.J. and Kim,D.H. Inhibition of thermolysin with nitrone-bearing substrate analogs: a new type of thermolysin inhibitors Bioorg Med Chem Lett (1998) 8, 323-326. PubMed Europe PubMed DOI I
[YEAR:6-11-1998]Marie-Claire,C., Ruffet,E., Tiraboschi,G. and Fournie-Zaluski,M.C. Differences in transition state stabilization between thermolysin (EC 3.4.24.27) and neprilysin (EC 3.4.24.11) FEBS Lett (6-11-1998) 438, 215-219. PubMed Europe PubMed DOI
[YEAR:6-3-1998]Marie-Claire,C., Roques,B.P. and Beaumont,A. Intramolecular processing of prothermolysin J Biol Chem (6-3-1998) 273, 5697-5701. PubMed Europe PubMed DOI
Mejri,M., Pauthe,E., Larreta-Garde,V. and Mathlouthi,M. Effect of polyhydroxylic additives on the catalytic activity of thermolysin Enzyme Microb Technol (1998) 23, 392-396. DOI
[YEAR:18-9-1998]Tate,S., Ohno,A., Seeram,S.S., Hiraga,K., Oda,K. and Kainosho,M. Elucidation of the mode of interaction of thermolysin with a proteinaceous metalloproteinase inhibitor, SMPI, based on a model complex structure and a structural dynamics analysis J Mol Biol (18-9-1998) 282, 435-446. PubMed Europe PubMed DOI
[YEAR:3-3-1998]Van Den Burg,B., Vriend,G., Veltman,O.R., Venema,G. and Eijsink,V.G. Engineering an enzyme to resist boiling Proc Natl Acad Sci U S A (3-3-1998) 95, 2056-2060. PubMed Europe PubMed DOI PMC EPMC

1997

Donovan,W.P., Tan,Y. and Slaney,A.C. Cloning of the nprA gene for neutral protease a of Bacillus thuringiensis and effect of in vivo deletion of nprA on insecticidal crystal protein Appl Environ Microbiol (1997) 63, 2311-2317. PubMed Europe PubMed PMC EPMC
Gresh,N. and Roques,B.P. Thermolysin-inhibitor binding: effect of the His231-- >Ala mutation on the relative affinities of thiolate versus phosphoramidate inhibitors - a model theoretical investigation incorporating a Continuum reaction field hydration model Biopolymers (1997) 41, 145-164. I
Inouye,K., Lee,S.B., Nambu,K. and Tonomura,B. Effects of pH, temperature, and alcohols on the remarkable activation of thermolysin by salts J Biochem (1997) 122, 358-364. PubMed Europe PubMed

1996

Daniel,R.M., Toogood,H.S. and Bergquist,P.L. Thermostable proteases Biotechnol Genet Eng Rev (1996) 13, 51-100. PubMed Europe PubMed V
Kim,D.H. and Jin,Y. Inhibitory stereochemistry of N-chloroacetyl-N-hydroxyleucine methyl ester for thermolysin Bioorg Med Chem Lett (1996) 6, 153-156. DOI I
Lister,S.A., Wetmore,D.R., Roche,R.S. and Codding,P.W. E144S Active-site mutant of the Bacillus cereus thermolysin-like neutral protease at 2.8 A resolution Acta Crystallogr D Biol Crystallogr (1996) 52, 543-550. PubMed Europe PubMed DOI S
Mock,W.L. and Stanford,D.J. Arazoformyl dipeptide substrates for thermolysin. Confirmation of a reverse protonation catalytic mechanism Biochemistry (1996) 35, 7369-7377. PubMed Europe PubMed DOI
Murakami,Y., Hirata,M. and Hirata,A. Mathematical approach to thermolysin catalyzed synthesis of aspartame precursor J Ferment Bioeng (1996) 82, 246-252.
O'Donohue,M.J. and Beaumont,A. The roles of the prosequence of thermolysin in enzyme inhibition and folding in vitro J Biol Chem (1996) 271, 26477-26481. PubMed Europe PubMed DOI
Spungin-Bialik,A., Ben-Meir,D., Fudim,E., Carmeli,S. and Blumberg,S. Sensitive substrates for neprilysin (neutral endopeptidase) and thermolysin that are highly resistant to serine proteases FEBS Lett (1996) 380, 79-82. PubMed Europe PubMed DOI A

1995

Beaumont,A., O'Donohue,M.J., Paredes,N., Rousselet,N., Assicot,M., Bohuon,C., Fournie-Zaluski,M.C. and Roques,B.P. The role of histidine 231 in thermolysin-like enzymes. A site-directed mutagenesis study J Biol Chem (1995) 270, 16803-16808. PubMed Europe PubMed DOI
Persichetti,R.A., Clair,N.L., Griffith,J.P., Navia,M.A. and Margolin,A.L. Cross-linked enzyme crystals (CLECs) of thermolysin in the synthesis of peptides J Am Chem Soc (1995) 117, 2732-2737.
van Aalten,D.M., Amadei,A., Linssen,A.B., Eijsink,V.G., Vriend,G. and Berendsen,H.J. The essential dynamics of thermolysin: confirmation of the hinge-bending motion and comparison of simulations in vacuum and water Proteins (1995) 22, 45-54. PubMed Europe PubMed DOI
Vecerek,B. and Kyslik,P. Cloning and sequencing of the neutral protease-encoding gene from a thermophilic strain of Bacillus sp Gene (1995) 158, 147-148. PubMed Europe PubMed DOI

1994

Holland,D.R., Barclay,P.L., Danilewicz,J.C., Matthews,B.W. and James,K. Inhibition of thermolysin and neutral endopeptidase 24.11 by a novel glutaramide derivative: X-ray structure determination of the thermolysin-inhibitor complex Biochemistry (1994) 33, 51-56. PubMed Europe PubMed DOI S I
Inagaki,T., Tadasa,K. and Kayahara,H. Modification effects of organic solvents on microenvironment of the enzyme in thermolysin-catalyzed peptide synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-phenylalanine methyl ester Biosci Biotechnol Biochem (1994) 58, 1439-1442. DOI
O'Donohue,M.J., Roques,B.P. and Beaumont,A. Cloning and expression in Bacillus subtilis of the npr gene from Bacillus thermoproteolyticus Rokko coding for the thermostable metalloprotease thermolysin Biochem J (1994) 300, 599-603. PubMed Europe PubMed PMC EPMC

1993

Kuhn,S. and Fortnagel,P. Molecular cloning and nucleotide sequence of the gene encoding a calcium-dependent exoproteinase from Bacillus megaterium ATCC 14581 J Gen Microbiol (1993) 139, 39-47. PubMed Europe PubMed
Vriend,G. and Eijsink,V. Prediction and analysis of structure, stability and unfolding of thermolysin-like proteases J Comput Aided Mol Des (1993) 7, 367-396. PubMed Europe PubMed V

1992

Eijsink,V.G., Vriend,G., van der Vinne,B., Hazes,B., Van Den Burg,B. and Venema,G. Effects of changing the interaction between subdomains on the thermostability of Bacillus neutral proteases Proteins (1992) 14, 224-236. PubMed Europe PubMed DOI
[YEAR:24-11-1992]Holland,D.R., Tronrud,D.E., Pley,H.W., Flaherty,K.M., Stark,W., Jansonius,J.N., McKay,D.B. and Matthews,B.W. Structural comparison suggests that thermolysin and related neutral proteases undergo hinge-bending motion during catalysis Biochemistry (24-11-1992) 31, 11310-11316. PubMed Europe PubMed S
Inouye,K. Effects of salts on thermolysin: activation of hydrolysis and synthesis of N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester, and a unique change in the absorption spectrum of thermolysin J Biochem (1992) 112, 335-340. PubMed Europe PubMed
Stark,W., Pauptit,R.A., Wilson,K.S. and Jansonius,J.N. The structure of neutral protease from Bacillus cereus at 0.2-nm resolution Eur J Biochem (1992) 207, 781-791. PubMed Europe PubMed DOI S

1991

Eijsink,V.G., Van Den Burg,B. and Venema,G. High performance affinity chromatography of Bacillus neutral proteases Biotechnol Appl Biochem (1991) 14, 275-283. PubMed Europe PubMed
Morgan,B.P., Scholtz,J.M., Ballinger,M.D., Zipkin,I.D. and Bartlett,P.A. Differential binding energy: a detailed evaluation of the influence of hydrogen-bonding and hydrophobic groups on the inhibition of thermolysin by phosphorus-containing inhibitors J Am Chem Soc (1991) 113, 297-307. I
Van Den Burg,B., Enequist,H.G., van der Haar,M.E., Eijsink,V.G., Stulp,B.K. and Venema,G. A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product J Bacteriol (1991) 173, 4107-4115. PubMed Europe PubMed PMC EPMC

1990

Eijsink,V.G., Vriend,G., Van Den Burg,B., Venema,G. and Stulp,B.K. Contribution of the C-terminal amino acid to the stability of Bacillus subtilis neutral protease Protein Eng (1990) 4, 99-104. PubMed Europe PubMed
Stoeva,S., Kleinschmidt,T., Mesrob,B. and Braunitzer,G. Primary structure of a zinc protease from Bacillus mesentericus strain 76 Biochemistry (1990) 29, 527-534. PubMed Europe PubMed DOI

1989

[YEAR:13-6-1989]Grobelny,D., Goli,U.B. and Galardy,R.E. Binding energetics of phosphorus-containing inhibitors of thermolysin Biochemistry (13-6-1989) 28, 4948-4951. PubMed Europe PubMed I
Merz,K. and Kollman,P. Free energy perturbation simulations of the inhibition of thermolysin: prediction of the free energy of binding of a new inhibitor J Am Chem Soc (1989) 111, 5649-5658. I
[YEAR:21-2-1989]Roderick,S.L., Fournie-Zaluski,M.C., Roques,B.P. and Matthews,B.W. Thiorphan and retro-thiorphan display equivalent interactions when bound to crystalline thermolysin Biochemistry (21-2-1989) 28, 1493-1497. PubMed Europe PubMed DOI S I
Toma,S., Campagnoli,S., De Gregoriis,E., Gianna,R., Margarit,I., Zamai,M. and Grandi,G. Effect of Glu-143 and His-231 substitutions on the catalytic activity and secretion of Bacillus subtilis neutral protease Protein Eng (1989) 2, 359-364. PubMed Europe PubMed
Van Den Burg,B., Eijsink,V.G., Stulp,B.K. and Venema,G. One-step affinity purification of Bacillus neutral proteases using bacitracin-silica J Biochem Biophys Methods (1989) 18, 209-219. PubMed Europe PubMed I

1988

Fontana,A. Structure and stability of thermophilic enzymes. Studies on thermolysin Biophys Chem (1988) 29, 181-193. PubMed Europe PubMed
[YEAR:25-7-1988]Gettins,P. Thermolysin-inhibitor complexes examined by 31P and 113Cd NMR spectroscopy J Biol Chem (25-7-1988) 263, 10208-10211. PubMed Europe PubMed I
Holden,H.M. and Matthews,B.W. The binding of L-valyl-L-tryptophan to crystalline thermolysin illustrates the mode of interaction of a product of peptide hydrolysis J Biol Chem (1988) 263, 3256-3260. PubMed Europe PubMed S
Matthews,B.W. Structural basis of the action of thermolysin and related zinc peptidases Acc Chem Res (1988) 21, 333-340. S
Pauptit,R.A., Karlsson,R., Picot,D., Jenkins,J.A., Niklaus-Reimer,A.S. and Jansonius,J.N. Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin J Mol Biol (1988) 199, 525-537. PubMed Europe PubMed S

1987

[YEAR:29-12-1987]Bartlett,P.A. and Marlowe,C.K. Possible role for water dissociation in the slow binding of phosphorus-containing transition-state-analogue inhibitors of thermolysin Biochemistry (29-12-1987) 26, 8553-8561. PubMed Europe PubMed I
[YEAR:29-12-1987]Holden,H.M., Tronrud,D.E., Monzingo,A.F., Weaver,L.H. and Matthews,B.W. Slow- and fast-binding inhibitors of thermolysin display different modes of binding: crystallographic analysis of extended phosphonamidate transition-state analogues Biochemistry (29-12-1987) 26, 8542-8553. PubMed Europe PubMed I
[YEAR:30-1-1987]Tronrud,D.E., Holden,H.M. and Matthews,B.W. Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond Science (30-1-1987) 235, 571-574. PubMed Europe PubMed I

1986

Hersh,L.B. and Morihara,K. Comparison of the subsite specificity of the mammalian neutral endopeptidase 24.11 (enkephalinase) to the bacterial neutral endopeptidase thermolysin J Biol Chem (1986) 261, 6433-6437. PubMed Europe PubMed P
Sidler,W., Niederer,E., Suter,F. and Zuber,H. The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase Biol Chem Hoppe Seyler (1986) 367, 643-657. PubMed Europe PubMed
[YEAR:2-6-1986]Tronrud,D.E., Monzingo,A.F. and Matthews,B.W. Crystallographic structural analysis of phosphoramidates as inhibitors and transition-state analogs of thermolysin Eur J Biochem (2-6-1986) 157, 261-268. PubMed Europe PubMed S I

1985

Ooshima,H., Mori,H. and Harano,Y. Synthesis of aspartame precursor by thermolysin solid in organic solvent Biotechnol Lett (1985) 7, 789-792.
Takagi,M., Imanaka,T. and Aiba,S. Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus J Bacteriol (1985) 163, 824-831. PubMed Europe PubMed PMC EPMC

1984

Hangauer,D.G., Monzingo,A.F. and Matthews,B.W. An interactive computer graphics study of thermolysin-catalyzed peptide cleavage and inhibition by N-carboxymethyl dipeptides Biochemistry (1984) 23, 5730-5741. PubMed Europe PubMed I
Kawamura,F. and Doi,R.H. Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases J Bacteriol (1984) 160, 442-444. PubMed Europe PubMed PMC EPMC
Kitagishi,K. and Hiromi,K. Binding between thermolysin and its specific inhibitor, phosphoramidon J Biochem (1984) 95, 529-534. PubMed Europe PubMed I
Monzingo,A.F. and Matthews,B.W. Binding of N-carboxymethyl dipeptide inhibitors to thermolysin determined by X-ray crystallography: a novel class of transition- state analogues for zinc peptidases Biochemistry (1984) 23, 5724-5729. PubMed Europe PubMed S I

1983

[YEAR:4-1-1983]Holmes,M.A., Tronrud,D.E. and Matthews,B.W. Structural analysis of the inhibition of thermolysin by an active-site-directed irreversible inhibitor Biochemistry (4-1-1983) 22, 236-240. PubMed Europe PubMed I
Wayne,S.I. and Fruton,J.S. Thermolysin-catalyzed peptide bond synthesis Proc Natl Acad Sci U S A (1983) 80, 3241-3244. PubMed Europe PubMed PMC EPMC

1982

Holmes,M.A. and Matthews,B.W. Structure of thermolysin refined at 1.6 A resolution J Mol Biol (1982) 160, 623-639. PubMed Europe PubMed S
Kunugi,S., Hirohara,H. and Ise,N. pH and temperature dependences of thermolysin catalysis. Catalytic role of zinc-coordinated water Eur J Biochem (1982) 124, 157-163. PubMed Europe PubMed
[YEAR:6-7-1982]Monzingo,A.F. and Matthews,B.W. Structure of a mercaptan-thermolysin complex illustrates mode of inhibition of zinc proteases by substrate-analogue mercaptans Biochemistry (6-7-1982) 21, 3390-3394. PubMed Europe PubMed I

1981

[YEAR:24-11-1981]Holmes,M.A. and Matthews,B.W. Binding of hydroxamic acid inhibitors to crystalline thermolysin suggests a pentacoordinate zinc intermediate in catalysis Biochemistry (24-11-1981) 20, 6912-6920. PubMed Europe PubMed I

1979

[YEAR:10-2-1979]Bolognesi,M.C. and Matthews,B.W. Binding of the biproduct analog L-benzylsuccinic acid to thermolysin determined by X-ray crystallography J Biol Chem (10-2-1979) 254, 634-639. PubMed Europe PubMed S I
Holmquist,B. and Vallee,B.L. Metal-coordinating substrate analogs as inhibitors of metalloenzymes Proc Natl Acad Sci U S A (1979) 76, 6216-6220. PubMed Europe PubMed PMC EPMC I
Isowa,Y., Ichikawa,T. and Mori,K. The thermolysin catalyzed condensation reactions of N-substituted aspartic and glutamic acids with phenylalanine alkylesters Tetrahedron Lett (1979) 28, 2611-2612.
Nishino,N. and Powers,J.C. Design of potent reversible inhibitors for thermolysin. Peptides containing zinc coordinating ligands and their use in affinity chromatography Biochemistry (1979) 18, 4340-4347. PubMed Europe PubMed I

1978

Nishino,N. and Powers,J.C. Peptide hydroxamic acids as inhibitors of thermolysin Biochemistry (1978) 17, 2846-2850. PubMed Europe PubMed I
Rasnick,D. and Powers,J.C. Active site directed irreversible inhibition of thermolysin Biochemistry (1978) 17, 4363-4369. PubMed Europe PubMed I
Roche,R.S., Voordouw,G. and Matthews,B.W. The structural and functional roles of metal ions in thermolysin Crit Rev Biochem Mol Biol (1978) 5, 1-23. PubMed Europe PubMed DOI

1977

[YEAR:31-5-1977]Kester,W.R. and Matthews,B.W. Crystallographic study of the binding of dipeptide inhibitors to thermolysin: implications for the mechanism of catalysis Biochemistry (31-5-1977) 16, 2506-2516. PubMed Europe PubMed S I
[YEAR:10-11-1977]Kester,W.R. and Matthews,B.W. Comparison of the structures of carboxypeptidase A and thermolysin J Biol Chem (10-11-1977) 252, 7704-7710. PubMed Europe PubMed S
Priest,F.G. Extracellular enzyme synthesis in the genus Bacillus Bacteriol Rev (1977) 41, 711-753. PubMed Europe PubMed PMC EPMC
Weaver,L.H., Kester,W.R. and Matthews,B.W. A crystallographic study of the complex of phosphoramidon with thermolysin. A model for the presumed catalytic transition state and for the binding of extended substrates J Mol Biol (1977) 114, 119-132. PubMed Europe PubMed S I

1976

[YEAR:9-3-1976]Dahlquist,F.W., Long,J.W. and Bigbee,W.L. Role of calcium in the thermal stability of thermolysin Biochemistry (9-3-1976) 15, 1103-1111. PubMed Europe PubMed

1975

Komiyama,T., Suda,H., Aoyagi,T., Takeuchi,T. and Umezawa,H. Studies on inhibitory effect of phosphoramidon and its analogs on thermolysin Arch Biochem Biophys (1975) 171, 727-731. PubMed Europe PubMed I
Levy,P.L., Pangburn,M.K., Burstein,Y., Ericsson,L.H., Neurath,H. and Walsh,K.A. Evidence of homologous relationship between thermolysin and neutral protease A of Bacillus subtilis Proc Natl Acad Sci U S A (1975) 72, 4341-4345. PubMed Europe PubMed PMC EPMC

1974

Feder,J., Brougham,L.R. and Wildi,B.S. Inhibition of thermolysin by dipeptides Biochemistry (1974) 13, 1186-1189. PubMed Europe PubMed I
Holmquist,B. and Vallee,B.L. Metal substitutions and inhibition of thermolysin: spectra of the cobalt enzyme J Biol Chem (1974) 249, 4601-4607. PubMed Europe PubMed

1973

Fogarty,W.M. and Griffin,P.J. Production and purification of the metalloprotease of Bacillus polymyxa Appl Microbiol (1973) 26, 185-190. PubMed Europe PubMed PMC EPMC
Griffin,P.J. and Fogarty,W.M. Physiochemical properties of the native, zinc- and manganese-prepared metalloprotease of Bacillus polymyxa Appl Microbiol (1973) 26, 191-195. PubMed Europe PubMed PMC EPMC
Suda,H., Aoyagi,T., Takeuchi,T. and Umezawa,H. A thermolysin inhibitor produced by actinomycetes: phosphoramidon J Antibiot (Tokyo) (1973) 26, 621-623. PubMed Europe PubMed I

1972

Colman,P.M., Jansonius,J.N. and Matthews,B.W. The structure of thermolysin: an electron density map at 2.3_ resolution J Mol Biol (1972) 70, 701-724. PubMed Europe PubMed S
Matthews,B.W., Jansonius,J.N., Colman,P.M., Schoenborn,B.P. and Dupourque,D. Three-dimensional structure of thermolysin Nat New Biol (1972) 238, 37-41. PubMed Europe PubMed S
Matthews,B.W., Colman,P.M., Jansonius,J.N., Titani,K., Walsh,K.A. and Neurath,H. Structure of thermolysin Nat New Biol (1972) 238, 41-43. PubMed Europe PubMed S
Titani,K., Hermodson,M.A., Ericsson,L.H., Walsh,K.A. and Neurath,H. Amino-acid sequence of thermolysin Nat New Biol (1972) 238, 35-37. PubMed Europe PubMed
Titani,K., Hermodson,M.A., Ericsson,L.H., Walsh,K.A. and Neurath,H. Amino-acid sequence of thermolysin Nat New Biology (1972) 238, 58-59. DOI

1971

Feder,J., Keay,L., Garrett,L.R., Cirulis,N., Moseley,M.H. and Wildi,B.S. Bacillus cereus neutral protease Biochim Biophys Acta (1971) 251, 74-78. PubMed Europe PubMed
Feder,J., Garrett,L.R. and Wildi,B.S. Studies on the role of calcium in thermolysin Biochemistry (1971) 10, 4552-4556. PubMed Europe PubMed
Morihara,K. and Tsuzuki,H. Comparative study of various neutral proteinases from microorganisms: specificity with oligopeptides Arch Biochem Biophys (1971) 146, 291-296. PubMed Europe PubMed

1970

Matsubara,H. Purification and assay of thermolysin Methods Enzymol (1970) 19, 642-651. A
Morihara,K. and Tsuzuki,H. Thermolysin: kinetic study with oligopeptides Eur J Biochem (1970) 15, 374-380. PubMed Europe PubMed P
Wang,T.W. and Kassell,B. Digestion of the basic trypsin inhibitor of bovine pancreas by thermolysin Biochem Biophys Res Commun (1970) 40, 1039-1045. PubMed Europe PubMed

1969

Latt,S.A., Holmquist,B. and Vallee,B.L. Thermolysin: a zinc metalloenzyme Biochem Biophys Res Commun (1969) 37, 333-339. PubMed Europe PubMed
Millet,J., Acher,R. and Aubert,J.P. Biochemical and physiological properties of an extracellular protease produced by Bacillus megaterium Biotechnol Bioeng (1969) 11, 1233-1246. PubMed Europe PubMed DOI
Millet,J. and Aubert,J.P. [Study of megateriopeptidase, an exocellular peptidase of Bacillus megaterium. 3. Biosynthesis and physiological function] Ann Inst Pasteur (Paris) (1969) 117, 461-473. PubMed Europe PubMed
Millet,J. and Acher,R. [Specificity of megateriopeptidase: an amino-endopeptidase with hydrophobic characteristics] Eur J Biochem (1969) 9, 456-462. PubMed Europe PubMed
[YEAR:4-6-1969]Millet,J. [Study of megateriopeptidase, exocellular protease of Bacillus megaterium. 1. Purification and general properties] Bull Soc Chim Biol (Paris) (4-6-1969) 51, 61-68. PubMed Europe PubMed

1968

[YEAR:26-7-1968]Feder,J. A spectrophotometric assay for neutral protease Biochem Biophys Res Commun (26-7-1968) 32, 326-332. PubMed Europe PubMed
Millet,J. and Acher,R. ["Hydrophobic" specificity of an endopeptidase isolated from Bacillus megaterium] Biochim Biophys Acta (1968) 151, 302-305. PubMed Europe PubMed

1966

Matsubara,H., Sasaki,R., Singer,A. and Jukes,T.H. Specific nature of hydrolysis of insulin and tobacco mosaic virus protein by thermolysin Arch Biochem Biophys (1966) 115, 324-331. PubMed Europe PubMed
Matsubara,H. Observations on the specificity of thermolysin with synthetic peptides Biochem Biophys Res Commun (1966) 24, 427-430. PubMed Europe PubMed P
Ohta,Y., Ogura,Y. and Wada,A. Thermostable protease from thermophilic bacteria. I. Thermostability, physiochemical properties, and amino acid composition J Biol Chem (1966) 241, 5919-5925. PubMed Europe PubMed