$VAR1 = undef;

Summary for peptidase C13.004: legumain, animal-type

Names
MEROPS Name	legumain, animal-type
Other names	AEP, asparagine endopeptidase, asparaginyl endopeptidase (AEP), Alzheimer’s amyloid protein delta secretase, delta-secretase, LGMN g.p. (Homo sapiens), osteoclast inhibitory peptide 2, PRSC1 g.p. [obs.] (Homo sapiens)

Domain architecture

MEROPS Classification
Classification	Clan CD >> Subclan (none) >> Family C13 >> Subfamily (none) >> C13.004
Holotype	legumain, animal-type (Homo sapiens), Uniprot accession Q99538 (peptidase unit: 25-286), MERNUM MER0001800
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Cysteine
Peplist	Included in the Peplist with identifier PL00098
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.34
Enzymology	BRENDA database
Proteolytic events	CutDB database (17 cleavages)
Activity status	human: active (Chen et al., 1997) mouse: active (Chen et al., 1998)
Knockout	Knockout mice show that legumain is essential for processing of cathepsin L (C01.032), but not for class II MHC-restricted antigen presentation (Maehr et al., 2005).
Pathways	KEGG	Antigen processing and presentation
	KEGG	Lysosome
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/LGMN
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/-/-/ND-/-/-/- (based on 2266 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	178	225	127	12	254	184	169	170
Pro	170	171	145	5	55	170	183	195
Ala	195	258	211	14	196	187	197	188
Val	138	106	156	7	98	145	145	133
Leu	159	168	166	6	148	163	208	159
Ile	81	96	86	3	98	98	91	97
Met	55	43	57	9	54	33	40	41
Phe	71	70	50	5	72	55	65	55
Tyr	56	50	43	5	55	61	55	53
Trp	19	20	21	0	31	16	21	15
Ser	177	163	196	11	245	169	164	179
Thr	120	83	116	8	101	99	117	117
Cys	21	21	26	1	0	2	4	5
Asn	78	63	40	1483	60	37	44	49
Gln	106	110	101	7	88	94	105	114
Asp	149	175	100	669	169	141	119	107
Glu	200	189	279	5	176	255	187	184
Lys	115	130	184	6	180	181	172	174
Arg	120	99	141	6	140	135	136	166
His	50	25	20	4	41	36	38	56