Literature for peptidase C13.004: legumain, animal-type

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

(Topics flags: S Structure, A Assay, T Target, P Specificity, K Knockout, E Expression, V Review, I Inhibitor, L Localization, U Therapeutic. To select only the references relevant to a single topic, click the link above. See explanation.)

2026

He,Y., Jiang,K., Sun,J., Zhao,Q., Yue,J., Wei,W., Cao,J., Zheng,D., Yao,H., Zhao,S., Zhao,H. and Huang,E. METTL3-dependent N6-methyladenosine modification on LGMN mRNA promotes macrophage ferroptosis and atherosclerosis J Mol Cell Cardiol (2026) 212, 89-103. PubMed Europe PubMed DOI K
Zhang,J., Chen,X., Ming,R., Huang,L. and Zhang,R. Fluorescent light-up probes for legumain imaging in macrophages: Minimal disturbance to macrophage cytoskeleton Anal Chim Acta (2026) 1383, 344861-344861. PubMed Europe PubMed DOI

2025

Chen,J., Xu,W., Meng,L., Zhang,X., Lin,M., Zhang,S., Liu,Y. and Guo,F. The Combination of MIF Inhibitor and AEP Targeted Inhibitor to Reduce Lung Metastasis in Breast Cancer and Its Mechanism J Cell Mol Med (2025) 29, e70616-e70616. PubMed Europe PubMed DOI PMC EPMC
Enneking,A.E., Khorey,M.M. and Edgington-Mitchell,L.E. Nuclear Roles for Canonically Lysosomal Proteases Mol Cell Biol (2025) 45, 343-352. PubMed Europe PubMed DOI
Fuchigami,T., Itagaki,K., Yoshida,S., Nakayama,M., Munekane,M. and Ogawa,K. Synthesis and Evaluation of Radiogallium-Labeled Peptide Probes for In Vivo Imaging of Legumain Activity Molecules (2025) 30 PubMed Europe PubMed DOI PMC EPMC
Liu,Y., Liu,W., Guo,C., Bao,J., Zhang,H., Li,W., Li,Y., Gui,Y., Zeng,Y., Wang,X., Peng,C., Shu,X. and Liu,R. AENK ameliorates cognitive impairment and prevents Tau hyperphosphorylation through inhibiting AEP-mediated cleavage of SET in rats with ischemic stroke J Neurochem (2025) 169, e16283-e16283. PubMed Europe PubMed DOI
McGlinchey,R.P., Ramos,S., Dimitriadis,E.K., Wilson,C.B. and Lee,J.C. Defining essential charged residues in fibril formation of a lysosomal derived N-terminal alpha-synuclein truncation Nat Commun (2025) 16, 3825-3825. PubMed Europe PubMed DOI PMC EPMC
Morillo-Huesca,M., Lopez-Cepero,G., Conesa-Bakkali,R., Tome,M., Watts,C., Huertas,P., Moreno-Bueno,G., Duran,R.V. and Martinez-Fabregas,J. Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer J Exp Clin Cancer Res (2025) 44, 74-74. PubMed Europe PubMed DOI PMC EPMC
Schmidt,V., Ziemlinska,E., Obrebski,T., Gomes,J.P., Zurawska-Plaksej,E., Cendrowski,J., Palmfeldt,J., Hempstead,B.L., Willnow,T.E. and Malik,A.R. Astrocytes distress triggers brain pathology through induction of delta secretase in a murine model of Alzheimer's disease Nat Commun (2025) 16, 9653-9653. PubMed Europe PubMed DOI PMC EPMC
Shi,Z., Zhao,J., Lv,Q., Liao,K., Cao,L., Yang,J., Wang,M., Zhou,L., Xu,H., Ge,J., Qiu,Y., Chen,J., Chen,J., Ma,C. and Lin,Y. Chemotherapeutic drug-triggered AEP-cleaved G3BP1 orchestrates stress granules/nucleoli/mitochondria in osteosarcoma Bone Res (2025) 13, 74-74. PubMed Europe PubMed DOI PMC EPMC
Wang,X., Sun,J., Lan,B., Wang,C., Ma,J., Hu,Q., Liu,J., Cheng,H., Wen,H., Lin,J., Ren,X., Yu,H., Jiang,M., Chen,F., Ye,J., Zhai,J., Lan,H., Ouyang,K., Jing,Z., Lv,L., Chen,Y., Zhuo,W., Lin,J., Chen,Y., Lu,J., Shi,Y. and Wang,Y. Asparagine endopeptidase prompts breast cancer-related pericardial calcification by regulating IGF2 and integrin alphavbeta5 Proc Natl Acad Sci U S A (2025) 122, e2512936122-e2512936122. PubMed Europe PubMed DOI PMC EPMC
Xiang,X., Qin,M., Nie,L., Guo,X., Chen,J., Jiang,D., Zhang,Z. and Mao,L. FAM49B Fragmentation by Asparagine Endopeptidase Promotes Vascular Smooth Muscle Cell Migration in Atherogenesis Arterioscler Thromb Vasc Biol (2025) 45, e355-e355. PubMed Europe PubMed DOI
Zhao,J., Wu,L., Cai,G., Ou,D., Liao,K., Yang,J., Zhou,L., Huang,R., Lin,S., Huang,X., Lv,Q., Chen,J., Cao,L., Chen,J. and Lin,Y. Targeting PGE2 mediated senescent neuron improves tumour therapy Neuro Oncol (2025) PubMed Europe PubMed DOI
Ziegler,A.R., Parker,B.L., Scott,N.E. and Edgington-Mitchell,L.E. Employing complementary fractionation-based N-terminomics approaches enhances the identification of legumain cleavage events in naive and inflamed colon tissue Protein Sci (2025) 34, e70186-e70186. PubMed Europe PubMed DOI PMC EPMC

2024

Alexander,M.R. and Harrison,D.G. Legumain Regulates Regulatory T Cells in Hypertension Circ Res (2024) 134, 30-32. PubMed Europe PubMed DOI PMC EPMC
Forbord,K.M., Lunde,N.N., Bosnjak-Olsen,T., Johansen,H.T., Solberg,R. and Jafari,A. Legumain is a paracrine regulator of osteoblast differentiation and mediates the inhibitory effect of TGF-beta1 on osteoblast maturation Front Endocrinol (Lausanne) (2024) 15, 1445049-1445049. PubMed Europe PubMed DOI PMC EPMC
Hua,D., Xi,H., Xie,Q., Cai,S., Zhou,Y., Hu,X., Qiu,L. and Lin,J. Lysosome-targeting and legumain-triggered (68)Ga-labeled probe for enhanced tumor PET imaging Biochem Biophys Res Commun (2024) 703, 149646-149646. PubMed Europe PubMed DOI
Osoble,N.M.M., Lunde,N.N., Jafari,A., Thoresen,G.H., Solberg,R. and Rustan,A.C. The cysteine protease legumain decreases glucose metabolism and enhances fatty acid uptake in human myotubes Biochimie (2024) PubMed Europe PubMed DOI
Singh,M., Steinke,I. and Amin,R.H. Structure-based computer-aided drug design to identify potential lead molecules for Asparaginyl Endopeptidase inhibitors J Biomol Struct Dyn (2024) , 1-19. PubMed Europe PubMed DOI
Wan,X.C., Zhang,Y.N., Zhang,H., Chen,Y., Cui,Z.H., Zhu,W.J. and Fang,G.M. Asparaginyl Endopeptidase-Mediated Peptide Cyclization for Phage Display Org Lett (2024) 26, 2601-2605. PubMed Europe PubMed DOI
Xie,Y., Zhang,H. and Song,X. AEP promotes aberrant RNA splicing through DDX3X cleavage in solid tumors J Clin Invest (2024) 134 PubMed Europe PubMed DOI PMC EPMC
Xiong,M., Xia,D., Yu,H., Meng,L., Zhang,X., Chen,J., Tian,Y., Yuan,X., Niu,X., Nie,S., Zhang,Z., Liu,C., Chen,Q., Ye,K. and Zhang,Z. Microglia Process alpha-Synuclein Fibrils and Enhance their Pathogenicity in a TREM2-Dependent Manner Adv Sci (Weinh) (2024) , e2413451-e2413451. PubMed Europe PubMed DOI
Zhang,X., Zou,L., Tang,L., Xiong,M., Yan,X.X., Meng,L., Chen,G., Xiong,J., Nie,S., Zhang,Z., Chen,Q. and Zhang,Z. Bridging integrator 1 fragment accelerates tau aggregation and propagation by enhancing clathrin-mediated endocytosis in mice PLoS Biol (2024) 22, e3002470-e3002470. PubMed Europe PubMed DOI PMC EPMC
Zhou,L., Wu,J., Wei,Z. and Zheng,Y. Legumain in cardiovascular diseases Exp Biol Med (Maywood) (2024) 249, 10121-10121. PubMed Europe PubMed DOI PMC EPMC V
Ziegler,A.R., Anderson,B.M., Latorre,R., McQuade,R.M., Dufour,A., Schmidt,B.L., Bunnett,N.W., Scott,N.E. and Edgington-Mitchell,L.E. N-terminomics profiling of naive and inflamed murine colon reveals proteolytic signatures of legumain J Cell Physiol (2024) , e31466-e31466. PubMed Europe PubMed DOI
Ziegler,A.R., Dufour,A., Scott,N.E. and Edgington-Mitchell,L.E. Ion Mobility-Based Enrichment-Free N-Terminomics Analysis Reveals Novel Legumain Substrates in Murine Spleen Mol Cell Proteomics (2024) 23, 100714-100714. PubMed Europe PubMed DOI PMC EPMC

2023

Jiang,Y., Li,L., Wu,R., Wu,L., Zhang,B., Wang,J.Z., Liu,R., Liu,F., Wang,J. and Wang,X. c-Src regulates delta-secretase activation and truncated Tau production by phosphorylating the E3 ligase Traf6 J Biol Chem (2023) 299, 105462-105462. PubMed Europe PubMed DOI PMC EPMC K
Ke,B.Y., Qin,Y.S., Cheng,L. and Wang,S.Z. Legumain: a potential biomarker for atherosclerosis J Pharm Pharmacol (2023) 75, 1395-1404. PubMed Europe PubMed DOI V
Krummenacher,D., He,W., Kuhn,B., Schnider,C., Beurier,A., Brom,V., Sivasothy,T., Marty,C., Tosstorff,A., Hewings,D.S., Mesch,S., Pinard,E., Brandlin,M., Hochstrasser,R., Westwood,P., Rothe,J., Kronenberger,A., Morandi,F., Gutbier,S., Schuler,A., Heer,D., Gloria,L.E., Joedicke,L., Rudolph,M.G., Muller,L., Gruninger,F., Baumann,K., Kaniyappan,S., Manevski,N. and Bartels,B. Discovery of Orally Available and Brain Penetrant AEP Inhibitors J Med Chem (2023) 66, 17026-17043. PubMed Europe PubMed DOI
Mitchell,J., Colon,B., Bayik,D. and Lathia,J.D. The missing link? LGMN connects hypoxia and immunosuppression in glioblastoma Cell Rep Med (2023) 4, 101293-101293. PubMed Europe PubMed DOI PMC EPMC
Qiu,Y., Lei,C., Zeng,J., Xie,Y., Cao,Y., Yuan,Q., Su,H., Zhang,Z. and Zhang,C. Asparagine endopeptidase protects podocytes in adriamycin-induced nephropathy by regulating actin dynamics through cleaving transgelin Mol Ther (2023) 31, 3337-3354. PubMed Europe PubMed DOI PMC EPMC K
Wang,M., Tao,M., Zhu,W., Liu,W., Liu,Z. and Hai,Z. Tumor-Targeted Fluorescent/Photoacoustic Imaging of Legumain Activity In Vivo ACS Sens (2023) 8, 4473-4477. PubMed Europe PubMed DOI
Zhang,W., Cao,L., Yang,J., Zhang,S., Zhao,J., Shi,Z., Liao,K., Wang,H., Chen,B., Qian,Z., Xu,H., Wu,L., Liu,H., Wang,H., Ma,C., Qiu,Y., Ge,J., Chen,J. and Lin,Y. AEP-cleaved DDX3X induces alternative RNA splicing events to mediate cancer cell adaptation in harsh microenvironments J Clin Invest (2023) 134 PubMed Europe PubMed DOI PMC EPMC

2022

Elamin,T., Santos,N.P., Briza,P., Brandstetter,H. and Dall,E. Structural and functional studies of legumain-mycocypin complexes revealed a competitive, exosite-regulated mode of interaction J Biol Chem (2022) 298, 102502-102502. PubMed Europe PubMed DOI PMC EPMC
Jiang,S., Xiong,Y., Zhang,W., Zhu,J., Cheng,D., Gong,Y., Wu,Y., Qiao,H. and Fu,H. A Novel Legumain-Like Protease in Macrobrachium nipponense: Identification, Characterization, and Function Analysis in Ovary Maturation Front Endocrinol (Lausanne) (2022) 13, 858726-858726. PubMed Europe PubMed DOI PMC EPMC
Kovalyova,Y., Bak,D.W., Gordon,E.M., Fung,C., Shuman,J.H.B., Cover,T.L., Amieva,M.R., Weerapana,E. and Hatzios,S.K. An infection-induced oxidation site regulates legumain processing and tumor growth Nat Chem Biol (2022) 18, 698-705. PubMed Europe PubMed DOI
Lu,C., Wang,X., Wang,Q., Zhang,L., Lin,J. and Qiu,L. Development of a Promising (18)F-Radiotracer for PET Imaging Legumain Activity In Vivo Pharmaceuticals (Basel) (2022) 15 PubMed Europe PubMed DOI PMC EPMC
Pan,L., Bai,P., Weng,X., Liu,J., Chen,Y., Chen,S., Ma,X., Hu,K., Sun,A. and Ge,J. Legumain Is an Endogenous Modulator of Integrin alphavbeta3 Triggering Vascular Degeneration, Dissection, and Rupture Circulation (2022) 145, 659-674. PubMed Europe PubMed DOI
Xiong,J., Liao,J., Liu,X., Zhang,Z., Adams,J., Pacifici,R. and Ye,K. A TrkB agonist prodrug prevents bone loss via inhibiting asparagine endopeptidase and increasing osteoprotegerin Nat Commun (2022) 13, 4820-4820. PubMed Europe PubMed DOI PMC EPMC
Zhang,D., Wang,Z., Hu,S., Chan,N.Y., Liew,H.T., Lescar,J., Tam,J.P. and Liu,C.F. Asparaginyl Endopeptidase-Mediated Protein C-Terminal Hydrazinolysis for the Synthesis of Bioconjugates Bioconjug Chem (2022) 33, 238-247. PubMed Europe PubMed DOI

2021

Chen,C., Wang,D., Yu,Y., Zhao,T., Min,N., Wu,Y., Kang,L., Zhao,Y., Du,L., Zhang,M., Gong,J., Zhang,Z., Zhang,Y., Mi,X., Yue,S. and Tan,X. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI Cell Death Dis (2021) 12, 65-65. PubMed Europe PubMed DOI PMC EPMC I
Dall,E., Stanojlovic,V., Demir,F., Briza,P., Dahms,S.O., Huesgen,P.F., Cabrele,C. and Brandstetter,H. The Peptide Ligase Activity of Human Legumain Depends on Fold Stabilization and Balanced Substrate Affinities ACS Catal (2021) 11, 11885-11896. PubMed Europe PubMed DOI PMC EPMC
Wu,Z., Xia,Y., Wang,Z., Su Kang,S., Lei,K., Liu,X., Jin,L., Wang,X., Cheng,L. and Ye,K. C/EBPbeta/delta-secretase signaling mediates Parkinson's disease pathogenesis via regulating transcription and proteolytic cleavage of alpha-synuclein and MAOB Mol Psychiatry (2021) 26, 568-585. PubMed Europe PubMed DOI
Xia,Y., Wang,Z.H., Liu,P., Edgington-Mitchell,L., Liu,X., Wang,X.C. and Ye,K. TrkB receptor cleavage by delta-secretase abolishes its phosphorylation of APP, aggravating Alzheimer's disease pathologies Mol Psychiatry (2021) 26, 2943-2963. PubMed Europe PubMed DOI
Xia,Y., Wang,Z.H., Zhang,Z., Liu,X., Yu,S.P., Wang,J.Z., Wang,X.C. and Ye,K. Delta- and beta- secretases crosstalk amplifies the amyloidogenic pathway in Alzheimer's disease Prog Neurobiol (2021) , 102113-102113. PubMed Europe PubMed DOI
Zhang,W. and Lin,Y. The Mechanism of Asparagine Endopeptidase in the Progression of Malignant Tumors: A Review Cells (2021) 10 PubMed Europe PubMed DOI PMC EPMC V
Zhang,X., Tang,L. and Zhang,Z. ADAM10 and ADAM17 are degraded by lysosomal pathway via asparagine endopeptidase Biochem Biophys Res Commun (2021) 537, 15-21. PubMed Europe PubMed DOI
Zou,L., Zhang,X., Xiong,M., Meng,L., Tian,Y., Pan,L., Yuan,X., Chen,G., Wang,Z., Bu,L., Yao,Z., Zhang,Z., Ye,K. and Zhang,Z. Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease Neurobiol Dis (2021) 154, 105326-105326. PubMed Europe PubMed DOI

2020

Amarnath,S. and Brown,M.L. Harnessing proteases for T regulatory cell immunotherapy Eur J Immunol (2020) 50, 770-778. PubMed Europe PubMed DOI
Chen,C., Ahn,E.H., Kang,S.S., Liu,X., Alam,A. and Ye,K. Gut dysbiosis contributes to amyloid pathology, associated with C/EBPbeta/AEP signaling activation in Alzheimer's disease mouse model Sci Adv (2020) 6, eaba0466-eaba0466. PubMed Europe PubMed DOI PMC EPMC
Chen,H.C., Rui,W., You,S.Y., Liu,X.W., Huang,J. and Chen,H.Y. Evaluation of the anti-cervical cancer effect of a prodrug CBZ-AAN-DOX with hypoxic cell culture and tumor-bearing zebrafish models Exp Cell Res (2020) , 111980-111980. PubMed Europe PubMed DOI
Chen,R., Zhang,Q., Yan,Y., Zhang,Y. and Zhang,T. Legumain Knockout Protects Against Abeta1-42-Induced AD-like Cognitive Deficits and Synaptic Plasticity Dysfunction Via Inhibiting Neuroinflammation Without Cleaving APP Mol Neurobiol (2020) PubMed Europe PubMed DOI
de Jager,S.C. and Hoefer,I.E. Legumain in cardiovascular disease: culprit or ally? Atherosclerosis (2020) 296, 66-67. PubMed Europe PubMed DOI
English,A.W., Liu,X., Mistretta,O.C., Ward,P.J. and Ye,K. Asparagine endopeptidase (delta secretase), an enzyme implicated in Alzheimer's disease pathology, is an inhibitor of axon regeneration in peripheral nerves eNeuro (2020) PubMed Europe PubMed DOI
Hou,L., Yan,Y., Tian,C., Huang,Q., Fu,X., Zhang,Z., Zhang,H., Zhang,H. and Zhang,Z. Single-dose in situ storage for intensifying anticancer efficacy via combinatorial strategy J Control Release (2020) 319, 438-449. PubMed Europe PubMed DOI
Kang,S.S., Liu,X., Ahn,E.H., Xiang,J., Manfredsson,F.P., Yang,X., Luo,H.R., Liles,L.C., Weinshenker,D. and Ye,K. Norepinephrine metabolite DOPEGAL activates AEP and pathological Tau aggregation in locus coeruleus J Clin Invest (2020) 130, 422-437. PubMed Europe PubMed DOI
Kang,S.S., Ahn,E.H. and Ye,K. Delta-secretase cleavage of Tau mediates its pathology and propagation in Alzheimer's disease Exp Mol Med (2020) 52, 1275-1287. PubMed Europe PubMed DOI V
Kim,N. and Lee,H.J. Target Enzymes Considered for the Treatment of Alzheimer's Disease and Parkinson's Disease Biomed Res Int (2020) 2020, 2010728-2010728. PubMed Europe PubMed DOI PMC EPMC V
Kremer,A.N., Bausenwein,J., Lurvink,E., Kremer,A.E., Rutten,C.E., van Bergen,C.A.M., Kretschmann,S., van der Meijden,E., Honders,M.W., Mazzeo,D., Watts,C., Mackensen,A., Falkenburg,J.H.F. and Griffioen,M. Discovery and differential processing of HLA class II-restricted minor histocompatibility antigen LB-PIP4K2A-1S and its allelic variant by asparagine endopeptidase Front Immunol (2020) 11, 381-381. PubMed Europe PubMed DOI PMC EPMC P
Lerchen,H.G., Stelte-Ludwig,B., Sommer,A., Berndt,S., Rebstock,A.S., Johannes,S., Mahlert,C., Greven,S., Dietz,L. and Jorissen,H. Tailored Linker Chemistries for the Efficient and Selective Activation of ADCs with KSPi Payloads Bioconjug Chem (2020) PubMed Europe PubMed DOI
Li,X., Tang,M., Zhu,Q., Wang,X., Lin,Y. and Wang,X. The exosomal integrin alpha5beta1/AEP complex derived from epithelial ovarian cancer cells promotes peritoneal metastasis through regulating mesothelial cell proliferation and migration Cell Oncol (Dordr) (2020) 43, 263-277. PubMed Europe PubMed DOI
Li,Y., Niu,Y., Zhu,J., Gao,C., Xu,Q., He,Z., Chen,D., Xu,M. and Liu,Y. Tailor-made legumain/pH dual-responsive doxorubicin prodrug-embedded nanoparticles for efficient anticancer drug delivery and in situ monitoring of drug release Nanoscale (2020) 12, 2673-2685. PubMed Europe PubMed DOI
Liao,K., Qian,Z., Zhang,S., Chen,B., Li,Z., Huang,R., Cheng,L., Wang,T., Yang,R., Lan,J., Lu,X., Kong,L., Song,X., Qiu,Y. and Lin,Y. The LGMN pseudogene promotes tumor progression by acting as a miR-495-3p sponge in glioblastoma Cancer Lett (2020) 490, 111-123. PubMed Europe PubMed DOI
Lin,Y., Liao,K., Miao,Y., Qian,Z., Fang,Z., Yang,X., Nie,Q., Jiang,G., Liu,J., Yu,Y., Wan,J., Zhang,X., Hu,Y., Jiang,J. and Qiu,Y. Role of asparagine endopeptidase in mediating wild-type p53 inactivation of glioblastoma J Natl Cancer Inst (2020) 112, 343-355. PubMed Europe PubMed DOI
Liu,Y., Guo,C., Ding,Y., Long,X., Li,W., Ke,D., Wang,Q., Liu,R., Wang,J.Z., Zhang,H. and Wang,X. Blockage of AEP attenuates TBI-induced tau hyperphosphorylation and cognitive impairments in rats Aging (Albany NY) (2020) 12, 19421-19439. PubMed Europe PubMed DOI
Lunde,N.N., Gregersen,I., Ueland,T., Shetelig,C., Holm,S., Kong,X.Y., Michelsen,A.E., Otterdal,K., Yndestad,A., Broch,K., Gullestad,L., Nyman,T.A., Bendz,B., Eritsland,J., Hoffmann,P., Skagen,K., Goncalves,I., Nilsson,J., Grenegard,M., Poreba,M., Drag,M., Seljeflot,I., Sporsheim,B., Espevik,T., Skjelland,M., Johansen,H.T., Solberg,R., Aukrust,P., Bjorkbacka,H., Andersen,G.O. and Halvorsen,B. Legumain is upregulated in acute cardiovascular events and associated with improved outcome - potentially related to anti-inflammatory effects on macrophages Atherosclerosis (2020) 296, 74-82. PubMed Europe PubMed DOI
Manfredi,J.J. Inactivation of wild-type p53 by asparagine endopeptidase in glioblastoma: an opportunity to target the "undruggable" J Natl Cancer Inst (2020) 112, 327-329. PubMed Europe PubMed DOI T
Reddy,B.D., Beeraka,N.M., Chitturi,C.M.K. and Madhunapantula,S.V. An Overview on Targeting Legumain for Inhibiting Cancers Curr Pharm Des (2020) PubMed Europe PubMed DOI K V
Ren,Y.C., Zhao,Q., He,Y., Li,B., Wu,Z., Dai,J., Wen,L., Wang,X. and Hu,G. Legumain promotes fibrogenesis in chronic pancreatitis via activation of transforming growth factor beta1 J Mol Med (Berl) (2020) PubMed Europe PubMed DOI T I
Shen,L., Kang,L., Wang,D., Xun,J., Chen,C., Du,L., Zhang,M., Gong,J., Mi,X., Yue,S., Zhang,Y., Song,X., Xiang,R., Zhang,Z. and Tan,X. Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation Cancer Lett (2020) 472, 40-49. PubMed Europe PubMed DOI K
Soh,W.T., Demir,F., Dall,E., Perrar,A., Dahms,S.O., Kuppusamy,M., Brandstetter,H. and Huesgen,P.F. Extending proteome coverage with legumain as a highly specific digestion protease Anal Chem (2020) 92, 2961-2971. PubMed Europe PubMed DOI
Umei,T.C., Kishimoto,Y., Aoyama,M., Saita,E., Niki,H., Ikegami,Y., Ohmori,R., Kondo,K. and Momiyama,Y. High plasma levels of legumain in patients with complex coronary lesions J Atheroscler Thromb (2020) 27, 711-717. PubMed Europe PubMed DOI
Wang,H., Chen,B., Lin,Y., Zhou,Y. and Li,X. Legumain promotes gastric cancer progression through tumor-associated macrophages in vitro and in vivo Int J Biol Sci (2020) 16, 172-180. PubMed Europe PubMed DOI PMC EPMC K
Wang,J., Wang,Y., Cao,H., Wang,H., Li,J., Li,Y., Li,Y. and Zhang,Z. Orally delivered legumain-activated nanovehicles improve tumor accumulation and penetration for combinational photothermal-chemotherapy J Control Release (2020) 323, 59-70. PubMed Europe PubMed DOI U
Wang,Y., Zhang,S., Wang,H., Cui,Y., Wang,Z., Cheng,X., Li,W., Hou,J., Ji,Y. and Liu,T. High Level of Legumain Was Correlated With Worse Prognosis and Peritoneal Metastasis in Gastric Cancer Patients Front Oncol (2020) 10, 966-966. PubMed Europe PubMed DOI PMC EPMC
Yu,T., Wan,F., Liu,C., Zhang,X., Liu,Z., Zhang,J., Xiong,J., Wang,T. and Zhang,Z. Asparagine endopeptidase inhibitor protects against fenpropathrin-induced neurodegeneration via suppressing alpha-synuclein aggregation and neuroinflammation Eur J Pharmacol (2020) 888, 173586-173586. PubMed Europe PubMed DOI
Zhan,J., Zhong,J., Ma,S., Ma,W., Wang,Y., Yu,Z., Cai,Y. and Huang,W. Dual-responsive self-assembly in lysosomes enables cell cycle arrest for locking glioma cell growth Chem Commun (Camb) (2020) 56, 6957-6960. PubMed Europe PubMed DOI
Zhang,Z., Tian,Y. and Ye,K. delta-Secretase in neurodegenerative diseases: mechanisms, regulators and therapeutic opportunities Transl Neurodegener (2020) 9, 1-1. PubMed Europe PubMed DOI PMC EPMC
Zhao,T., Zhou,H., Lei,L., Guo,C., Yang,Q., Gong,T., Sun,X., Song,X., Gong,T. and Zhang,Z. A new tandem peptide modified liposomal doxorubicin for tumor "ecological therapy" Nanoscale (2020) 12, 3359-3369. PubMed Europe PubMed DOI

2019

Bai,P., Lyu,L., Yu,T., Zuo,C., Fu,J., He,Y., Wan,Q., Wan,N., Jia,D. and Lyu,A. Macrophage-derived legumain promotes pulmonary hypertension by activating the MMP (matrix metalloproteinase)-2/TGF (transforming growth factor)-beta1 signaling Arterioscler Thromb Vasc Biol (2019) 39, e130-e130. PubMed Europe PubMed DOI
Behrendt,A., Bichmann,M., Ercan-Herbst,E., Haberkant,P., Schondorf,D.C., Wolf,M., Fahim,S.A., Murolo,E. and Ehrnhoefer,D.E. Asparagine endopeptidase cleaves tau at N167 after uptake into microglia Neurobiol Dis (2019) 130, 104518-104518. PubMed Europe PubMed DOI
Chen,X., Wang,C., Liao,K., Zhou,S., Cao,L., Chen,J., Xu,C. and Lin,Y. USP17 suppresses tumorigenesis and tumor growth through deubiquitinating AEP Int J Biol Sci (2019) 15, 738-748. PubMed Europe PubMed DOI PMC EPMC
Eddie,S.L., Gregson,A., Graham,E., Burton,S., Harrison,T., Burden,R., Scott,C.J., Mullan,P.B. and Williams,R. Identification and SAR exploration of a novel series of legumain inhibitors Bioorg Med Chem Lett (2019) 29, 1546-1548. PubMed Europe PubMed DOI I
Fang,Y., Duan,C., Chen,S., Xie,P., Ai,W., Wang,L., Liu,R. and Fang,H. Increased legumain/Smad3 expression in atherosclerotic plaque of rat thoracic aorta Biomed Pharmacother (2019) 119, 109353-109353. PubMed Europe PubMed DOI
Fuchigami,T., Itagaki,K., Ishikawa,N., Yoshida,S. and Nakayama,M. Synthesis and evaluation of radioactive/fluorescent peptide probes for imaging of legumain activity Bioorg Med Chem Lett (2019) , 126629-126629. PubMed Europe PubMed DOI L
Kang,L., Shen,L., Lu,L., Wang,D., Zhao,Y., Chen,C., Du,L., Gong,J., Zhang,Y., Mi,X., Xiang,R., Zhang,M. and Tan,X. Asparaginyl endopeptidase induces endothelial permeability and tumor metastasis via downregulating zonula occludens protein ZO-1 Biochim Biophys Acta Mol Basis Dis (2019) 1865, 2267-2275. PubMed Europe PubMed DOI
Lerchen,H.G., Stelte-Ludwig,B., Berndt,S., Sommer,A., Dietz,L., Rebstock,A.S., Johannes,S., Marx,L., Jorissen,H., Mahlert,C. and Greven,S. Antibody-prodrug conjugates with KSP inhibitors and legumain-mediated metabolite formation Chemistry (2019) 25, 8208-8213. PubMed Europe PubMed DOI
Li,X., Liu,Q., Ye,S., Wang,S., Li,K., Lv,G., Peng,Y., Qiu,L. and Lin,J. A protease-responsive fluorescent probe for sensitive imaging of legumain activity in living tumor cells Chem Biol Drug Des (2019) 94, 1494-1503. PubMed Europe PubMed DOI L
Lunde,N.N., Bosnjak,T., Solberg,R. and Johansen,H.T. Mammalian legumain - a lysosomal cysteine protease with extracellular functions? Biochimie (2019) 166, 77-83. PubMed Europe PubMed DOI V
Ozawa,N., Sato,Y., Mori,Y., Masuda,H., Yamane,M., Yamamoto,Y., Shirai,R., Watanabe,R., Sato,K., Mori,Y., Hirano,T. and Watanabe,T. Legumain Promotes Atherosclerotic Vascular Remodeling Int J Mol Sci (2019) 20 PubMed Europe PubMed DOI
Poreba,M. Recent advances in the development of legumain-selective chemical probes and peptide prodrugs Biol Chem (2019) 400, 1529-1550. PubMed Europe PubMed DOI V I
Ruan,S., Xie,R., Qin,L., Yu,M., Xiao,W., Hu,C., Yu,W., Qian,Z., Ouyang,L., He,Q. and Gao,H. Aggregable nanoparticles-enabled chemotherapy and autophagy inhibition combined with anti-PD-L1 antibody for improved glioma treatment Nano Lett (2019) 19, 8318-8332. PubMed Europe PubMed DOI
Schlegel,K., Awwad,K., Heym,R.G., Holzinger,D., Doell,A., Barghorn,S., Jahn,T.R., Klein,C., Mordashova,Y., Schulz,M. and Gasparini,L. N368-Tau fragments generated by legumain are detected only in trace amount in the insoluble Tau aggregates isolated from AD brain Acta Neuropathol Commun (2019) 7, 177-177. PubMed Europe PubMed DOI PMC EPMC
Toss,M.S., Miligy,I.M., Gorringe,K.L., McCaffrey,L., AlKawaz,A., Abidi,A., Ellis,I.O., Green,A.R. and Rakha,E.A. Legumain is an independent predictor for invasive recurrence in breast ductal carcinoma in situ Mod Pathol (2019) 32, 639-649. PubMed Europe PubMed DOI
Wang,Y., Cui,Y., Wang,Z., Li,W. and Liu,T. Legumain is a predictive effector of peritoneal metastasis for diffuse gastric cancer patients Ann Oncol (2019) 30 Suppl 4, iv49-iv49. PubMed Europe PubMed DOI
Wrona,E., Jakubowska,J., Pawlik,B., Pastorczak,A., Madzio,J., Lejman,M., Sedek,L., Kowalczyk,J., Szczepanski,T. and Mlynarski,W. Gene expression of ASNS, LGMN and CTSB is elevated in a subgroup of childhood BCP-ALL with PAX5 deletion Oncol Lett (2019) 18, 6926-6932. PubMed Europe PubMed DOI PMC EPMC
Wu,Z., Wang,Z.H., Liu,X., Zhang,Z., Yu,S.P., Keene,C.D., Cheng,L. and Ye,K. Traumatic brain injury triggers APP and Tau cleavage by delta-secretase, mediating Alzheimer's disease pathology Prog Neurobiol (2019) , 101730-101730. PubMed Europe PubMed DOI
Xiang,J., Wang,Z.H., Ahn,E.H., Liu,X., Yu,S.P., Manfredsson,F.P., Sandoval,I.M., Ju,G., Wu,S. and Ye,K. Delta-secretase-cleaved Tau antagonizes TrkB neurotrophic signalings, mediating Alzheimer's disease pathologies Proc Natl Acad Sci U S A (2019) 116, 9094-9102. PubMed Europe PubMed DOI

2018

Basurto-Islas,G., Gu,J.H., Tung,Y.C., Liu,F. and Iqbal,K. Mechanism of tau hyperphosphorylation involving lysosomal enzyme asparagine endopeptidase in a mouse model of brain ischemia J Alzheimers Dis (2018) 63, 821-833. PubMed Europe PubMed DOI K
Freeley,S., Cardone,J., Gunther,S.C., West,E.E., Reinheckel,T., Watts,C., Kemper,C. and Kolev,M.V. Asparaginyl endopeptidase (legumain) supports human Th1 induction via cathepsin L-mediated intracellular C3 activation Front Immunol (2018) 9, 2449-2449. PubMed Europe PubMed DOI PMC EPMC
Gao,J., Li,K., Du,L., Yin,H., Tan,X. and Yang,Z. Deletion of asparagine endopeptidase reduces anxiety- and depressive-like behaviors and improves abilities of spatial cognition in mice Brain Res Bull (2018) 142, 147-155. PubMed Europe PubMed DOI K
Hu,W., Tung,Y.C., Zhang,Y., Liu,F. and Iqbal,K. Involvement of activation of asparaginyl endopeptidase in tau hyperphosphorylation in repetitive mild traumatic brain injury J Alzheimers Dis (2018) 64, 709-722. PubMed Europe PubMed DOI
Kang,S.S., Ahn,E.H., Zhang,Z., Liu,X., Manfredsson,F.P., Sandoval,I.M., Dhakal,S., Iuvone,P.M., Cao,X. and Ye,K. alpha-Synuclein stimulation of monoamine oxidase-B and legumain protease mediates the pathology of Parkinson's disease EMBO J (2018) 37, e98878-e98878. PubMed Europe PubMed DOI
Liu,X., Wang,Z., Zhang,G., Zhu,Q., Zeng,H., Wang,T., Gao,F., Qi,Z., Zhang,J. and Wang,R. Overexpression of asparaginyl endopeptidase is significant for esophageal carcinoma metastasis and predicts poor patient prognosis Oncol Lett (2018) 15, 1229-1235. PubMed Europe PubMed DOI PMC EPMC
Luo,M., Li,Q., Wang,D., Ge,C., Wang,J., Nan,K. and Lin,S. Fabrication of chitosan based nanocomposite with legumain sensitive properties using charge driven self-assembly strategy J Mater Sci Mater Med (2018) 29, 142-142. PubMed Europe PubMed DOI
Pahima,H., Reina,S., Tadmor,N., Dadon-Klein,D., Shteinfer-Kuzmine,A., Mazure,N.M., Pinto,V. and Shoshan-Barmatz,V. Hypoxic-induced truncation of voltage-dependent anion channel 1 is mediated by both asparagine endopeptidase and calpain 1 activities Oncotarget (2018) 9, 12825-12841. PubMed Europe PubMed DOI PMC EPMC I
Stathopoulou,C., Gangaplara,A., Mallett,G., Flomerfelt,F.A., Liniany,L.P., Knight,D., Samsel,L.A., Berlinguer-Palmini,R., Yim,J.J., Felizardo,T.C., Eckhaus,M.A., Edgington-Mitchell,L., Martinez-Fabregas,J., Zhu,J., Fowler,D.H., van Kasteren,S.I., Laurence,A., Bogyo,M., Watts,C., Shevach,E.M. and Amarnath,S. PD-1 Inhibitory receptor downregulates asparaginyl endopeptidase and maintains Foxp3 transcription factor stability in induced regulatory T cells Immunity (2018) 49, 247-263. PubMed Europe PubMed DOI K
Sun,W., Lin,Y., Chen,L., Ma,R., Cao,J., Yao,J., Chen,K. and Wan,J. Legumain suppresses OxLDL-induced macrophage apoptosis through enhancement of the autophagy pathway Gene (2018) 652, 16-24. PubMed Europe PubMed DOI
Wang,C., Zhang,J., Tang,J., Li,Y.Y., Gu,Y., Yu,Y., Xiong,J., Zhao,X., Zhang,Z., Li,T.T., Chen,J., Wan,Q. and Zhang,Z. Lysophosphatidic acid induces neuronal cell death via activation of asparagine endopeptidase in cerebral ischemia-reperfusion injury Exp Neurol (2018) 306, 1-9. PubMed Europe PubMed DOI
Wang,Z.H., Wu,W., Kang,S.S., Liu,X., Wu,Z., Peng,J., Yu,S.P., Manfredsson,F.P., Sandoval,I.M., Liu,X., Wang,J.Z. and Ye,K. BDNF inhibits neurodegenerative disease-associated asparaginyl endopeptidase activity via phosphorylation by AKT JCI Insight (2018) 3 PubMed Europe PubMed DOI
Wang,Z.H., Liu,P., Liu,X., Yu,S.P., Wang,J.Z. and Ye,K. Delta-secretase (AEP) mediates tau-splicing imbalance and accelerates cognitive decline in tauopathies J Exp Med (2018) 215, 3038-3056. PubMed Europe PubMed DOI
Yan,Q., Yuan,W.B., Sun,X., Zhang,M.J., Cen,F., Zhou,S.Y., Wu,W.B., Xu,Y.C., Tong,L.H. and Ma,Z.H. Asparaginyl endopeptidase enhances pancreatic ductal adenocarcinoma cell invasion in an exosome-dependent manner and correlates with poor prognosis Int J Oncol (2018) 52, 1651-1660. PubMed Europe PubMed DOI
Zhang,M., Jiang,Z., Chen,S., Wu,Z., Chen,K. and Wu,Y. Legumain correlates with neuroblastoma differentiation and can be used in prodrug design Chem Biol Drug Des (2018) 91, 534-544. PubMed Europe PubMed DOI
Zhang,X., Li,Z., Yang,H., Liu,D., Cai,G., Li,G., Mo,J., Wang,D., Zhong,C., Wang,H., Sun,Y., Shi,J., Zheng,E., Meng,F., Zhang,M., He,X., Zhou,R., Zhang,J., Huang,M., Zhang,R., Li,N., Fan,M., Yang,J. and Wu,Z. Novel transgenic pigs with enhanced growth and reduced environmental impact elife (2018) 7 PubMed Europe PubMed DOI PMC EPMC K
Zhang,Z., Li,X.G., Wang,Z.H., Song,M., Yu,S.P., Kang,S.S., Liu,X., Zhang,Z., Xie,M., Liu,G.P., Wang,J.Z. and Ye,K. delta-Secretase-cleaved Tau stimulates Abeta production via upregulating STAT1-BACE1 signaling in Alzheimer's disease Mol Psychiatry (2018) 26, 586-603. PubMed Europe PubMed DOI
Zhao,T., Li,Z., Guo,Z., Wang,A., Liu,Z., Zhao,Q., Li,Y., McKenzie,E.A. and Diao,A. Functional recombinant human legumain protein expression in Pichia pastoris to enable screening for legumain small molecule inhibitors Protein Expr Purif (2018) 150, 12-16. PubMed Europe PubMed DOI E
Zhao,Y., Hai,Z., Wang,H., Su,L. and Liang,G. Legumain-specific near-infrared fluorescence "turn on" for tumor-targeted imaging Anal Chem (2018) 90, 8732-8735. PubMed Europe PubMed DOI L

2017

Ammirati,E. and Fogacci,F. Clinical relevance of biomarkers for the identification of patients with carotid atherosclerotic plaque: potential role and limitations of cysteine protease legumain Atherosclerosis (2017) 257, 248-249. PubMed Europe PubMed DOI
Cui,Y., Li,Q., Li,H., Wang,Y., Wang,H., Chen,W., Zhang,S., Cao,J. and Liu,T. Asparaginyl endopeptidase improves the resistance of microtubule-targeting drugs in gastric cancer through IQGAP1 modulating the EGFR/JNK/ERK signaling pathway Onco Targets Ther (2017) 10, 627-643. PubMed Europe PubMed DOI PMC EPMC
Elsasser,B., Zauner,F.B., Messner,J., Soh,W.T., Dall,E. and Brandstetter,H. Distinct roles of catalytic cysteine and histidine in the protease and ligase mechanisms of human legumain as revealed by DFT-based QM/MM simulations ACS Catal (2017) 7, 5585-5593. PubMed Europe PubMed DOI PMC EPMC
Hong,J.A., Choi,N.E., La,Y.K., Nam,H.Y., Seo,J. and Lee,J. Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells Org Biomol Chem (2017) 15, 8018-8022. PubMed Europe PubMed DOI A L
Ilgova,J., Jedlickova,L., Dvorakova,H., Benovics,M., Mikes,L., Janda,L., Vorel,J., Roudnicky,P., Potesil,D., Zdrahal,Z., Gelnar,M. and Kasny,M. A novel type I cystatin of parasite origin with atypical legumain-binding domain Sci Rep (2017) 7, 17526-17526. PubMed Europe PubMed DOI PMC EPMC
Jafari,A., Qanie,D., Andersen,T.L., Zhang,Y., Chen,L., Postert,B., Parsons,S., Ditzel,N., Khosla,S., Johansen,H.T., Kjaersgaard-Andersen,P., Delaisse,J.M., Abdallah,B.M., Hesselson,D., Solberg,R. and Kassem,M. Legumain regulates differentiation fate of human bone marrow stromal cells and is altered in postmenopausal osteoporosis Stem Cell Reports (2017) 8, 373-386. PubMed Europe PubMed DOI
Lunde,N.N., Holm,S., Dahl,T.B., Elyouncha,I., Sporsheim,B., Gregersen,I., Abbas,A., Skjelland,M., Espevik,T., Solberg,R., Johansen,H.T. and Halvorsen,B. Increased levels of legumain in plasma and plaques from patients with carotid atherosclerosis Atherosclerosis (2017) 257, 216-223. PubMed Europe PubMed DOI
Lunde,N.N., Haugen,M.H., Bodin Larsen,K.B., Damgaard,I., Pettersen,S.J., Kasem,R., Rut,W., Drag,M., Poreba,M., Johansen,H.T. and Solberg,R. Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions Biochimie (2017) 139, 27-37. PubMed Europe PubMed DOI E
Mai,C.W., Chung,F.F. and Leong,C.O. Targeting legumain as a novel therapeutic strategy in cancers Curr Drug Targets (2017) 18, 1259-1268. PubMed Europe PubMed T I
Poreba,M., Szalek,A., Rut,W., Kasperkiewicz,P., Rutkowska-Wlodarczyk,I., Snipas,S.J., Itoh,Y., Turk,D., Turk,B., Overall,C.M., Kaczmarek,L., Salvesen,G.S. and Drag,M. Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families Sci Rep (2017) 7, 43135-43135. PubMed Europe PubMed DOI PMC EPMC A
Poreba,M., Salvesen,G.S. and Drag,M. Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity Nat Protoc (2017) 12, 2189-2214. PubMed Europe PubMed DOI P I
Qi,Q., Obianyo,O., Du,Y., Fu,H., Li,S. and Ye,K. Blockade of asparagine endopeptidase inhibits cancer metastasis J Med Chem (2017) 60, 7244-7255. PubMed Europe PubMed DOI I
Shi,T., Gu,L., Sun,Y., Wang,S., You,C., Zhang,X., Zhu,J. and Sun,B. Enhanced legumain-recognition and NIR controlled released of cisplatin-indocyanine nanosphere against gastric carcinoma Eur J Pharmacol (2017) 794, 184-192. PubMed Europe PubMed DOI U
Singh,Y., Pawar,V.K., Meher,J.G., Raval,K., Kumar,A., Shrivastava,R., Bhadauria,S. and Chourasia,M.K. Targeting tumor associated macrophages (TAMs) via nanocarriers J Control Release (2017) 254, 92-106. PubMed Europe PubMed DOI
Vidmar,R., Vizovisek,M., Turk,D., Turk,B. and Fonovic,M. Protease cleavage site fingerprinting by label-free in-gel degradomics reveals pH-dependent specificity switch of legumain EMBO J (2017) 36, 2455-2465. PubMed Europe PubMed DOI P
Wallin,H., Apelqvist,J., Andersson,F., Ekstrom,U. and Abrahamson,M. Low-level internalization of cystatin E/M affects legumain activity and migration of melanoma cells J Biol Chem (2017) 292, 14413-14424. PubMed Europe PubMed DOI
Xu,C., Cao,L., Liu,J., Qian,Z., Peng,Y., Zhu,W., Qiu,Y. and Lin,Y. Suppression of asparaginyl endopeptidase inhibits polyomavirus middle T antigen-induced tumor formation and metastasis Oncol Res (2017) 25, 407-415. PubMed Europe PubMed DOI I
Yamane,T., Kozuka,M., Yamamoto,Y., Nakano,Y., Nakagaki,T., Ohkubo,I. and Ariga,H. Protease activity of legumain is inhibited by an increase of cystatin E/M in the DJ-1-knockout mouse spleen, cerebrum and heart Biochem Biophys Rep (2017) 9, 187-192. PubMed Europe PubMed DOI
Yao,P., Ding,Y., Han,Z., Mu,Y., Hong,T., Zhu,Y. and Li,H. Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors Mol Pain (2017) 13, 1744806917708127-1744806917708127. PubMed Europe PubMed DOI PMC EPMC T
Zhang,Z., Obianyo,O., Dall,E., Du,Y., Fu,H., Liu,X., Kang,S.S., Song,M., Yu,S.P., Cabrele,C., Schubert,M., Li,X., Wang,J.Z., Brandstetter,H. and Ye,K. Inhibition of delta-secretase improves cognitive functions in mouse models of Alzheimer's disease Nat Commun (2017) 8, 14740-14740. PubMed Europe PubMed DOI S I
Zhou,H., Sun,H., Lv,S., Zhang,D., Zhang,X., Tang,Z. and Chen,X. Legumain-cleavable 4-arm poly(ethylene glycol)-doxorubicin conjugate for tumor specific delivery and release Acta Biomater (2017) 54, 227-238. PubMed Europe PubMed DOI
Zhu,Q., Tang,M. and Wang,X. The expression of asparaginyl endopeptidase promotes growth potential in epithelial ovarian cancer Cancer Biol Ther (2017) 18, 222-228. PubMed Europe PubMed DOI

2016

Cui,Y., Wang,Y., Li,H., Li,Q., Yu,Y., Xu,X., Xu,B. and Liu,T. Asparaginyl endopeptidase promotes the invasion and metastasis of gastric cancer through modulating epithelial-to-mesenchymal transition and analysis of their phosphorylation signaling pathways Oncotarget (2016) 7, 34356-34370. PubMed Europe PubMed DOI
Dall,E. and Brandstetter,H. Structure and function of legumain in health and disease Biochimie (2016) 122, 126-150. PubMed Europe PubMed DOI V
Dutta,A., Potier,D.N., Walker,M.J., Gray,O.J., Parker,C., Holland,M., Williamson,A.J., Pierce,A., Unwin,R.D., Krishnan,S., Saha,V. and Whetton,A.D. Development of a selected reaction monitoring mass spectrometry-based assay to detect asparaginyl endopeptidase activity in biological fluids Oncotarget (2016) 7, 70822-70831. PubMed Europe PubMed DOI A
Edgington-Mitchell,L.E., Wartmann,T., Fleming,A.K., Gocheva,V., van der Linden,W.A., Withana,N.P., Verdoes,M., Aurelio,L., Edgington-Mitchell,D., Lieu,T., Parker,B.S., Graham,B., Reinheckel,T., Furness,J.B., Joyce,J.A., Storz,P., Halangk,W., Bogyo,M. and Bunnett,N.W. Legumain is activated in macrophages during pancreatitis Am J Physiol Gastrointest Liver Physiol (2016) 311, G548-G560. PubMed Europe PubMed DOI
Hasegawa,S., Inoue,D., Yamasaki,M., Li,C., Imai,M., Takahashi,N. and Fukui,T. Site-specific cleavage of acetoacetyl-CoA synthetase by legumain FEBS Lett (2016) 590, 1592-1601. PubMed Europe PubMed DOI
Lin,S., Li,T., Xie,P., Li,Q., Wang,B., Wang,L., Li,L., Wang,Y., Chen,H. and Nan,K. Targeted delivery of doxorubicin to tumour tissues by a novel legumain sensitive polygonal nanogel Nanoscale (2016) 8, 18400-18411. PubMed Europe PubMed DOI
Meng,F. and Liu,W. Knockdown of legumain suppresses cervical cancer cell migration and invasion Oncol Res (2016) 23, 7-12. PubMed Europe PubMed DOI K
Ness,K.A., Eddie,S.L., Burton,S., Harrison,T., Mullan,P. and Williams,R. Flat SAR of P3-methylsulphonamide based small molecule legumain inhibitors Bioorg Med Chem Lett (2016) 26, 413-416. PubMed Europe PubMed DOI I
Poreba,M., Solberg,R., Rut,W., Lunde,N.N., Kasperkiewicz,P., Snipas,S.J., Mihelic,M., Turk,D., Turk,B., Salvesen,G.S. and Drag,M. Counter selection substrate library strategy for developing specific protease substrates and probes Cell Chem Biol (2016) 23, 1023-1035. PubMed Europe PubMed DOI A I
Shen,L., Li,H., Shi,Y., Wang,D., Gong,J., Xun,J., Zhou,S., Xiang,R. and Tan,X. M2 tumour-associated macrophages contribute to tumour progression via legumain remodelling the extracellular matrix in diffuse large B cell lymphoma Sci Rep (2016) 6, 30347-30347. PubMed Europe PubMed DOI I
Tan,Y., Nie,S., Zhu,W., Liu,F., Guo,H., Chu,J., Cao,X.B., Jiang,X., Zhang,Y. and Li,Y. 7,8-Dihydroxyflavone ameliorates cognitive impairment by inhibiting expression of tau pathology in ApoE-knockout mice Front Aging Neurosci (2016) 8, 287-287. PubMed Europe PubMed DOI PMC EPMC I
Wu,T., Sun,L., Wu,Y., Xiang,R., Li,Y., Rong,W., Sun,F. and Wang,N. Prognostic value of legumain in uveal melanoma Mol Med Rep (2016) 13, 2377-2384. PubMed Europe PubMed DOI
Yan,J.-S., Dong,B.-B., Chen,Q., Wang,L.-S., Wang,Q., Niu,X.-Z., Zheng,J.-H. and Yang,F.-Q. Up-regulation of legumain correlates with tumor progression and poor prognosis in urothelial carcinoma of bladder Int J Clin Exp Pathol (2016) 9, 12815-12821.
Zhang,Y., Wu,Y., Jiang,J., Liu,X., Ji,F.-J. and Fang,X.-D. MiRNA-3978 regulates peritoneal gastric cancer metastasis by targeting legumain Oncotarget (2016) 7, 83223-83230. PubMed Europe PubMed DOI
Zhang,Z., Xie,M. and Ye,K. Asparagine endopeptidase is an innovative therapeutic target for neurodegenerative diseases Expert Opin Ther Targets (2016) 20, 1237-1245. PubMed Europe PubMed DOI
Zhu,W., Shao,Y., Yang,M., Jia,M. and Peng,Y. Asparaginyl endopeptidase promotes proliferation and invasiveness of prostate cancer cells via PI3K/AKT signaling pathway Gene (2016) 594, 176-182. PubMed Europe PubMed DOI K

2015

Berven,L., Skjeldal,F.M., Prydz,K., Zubaidi,L.M.K., Ballance,S., ThidemannJohansen,H. and Samuelsen,A.B.C. Particulate yeast beta-glucan is internalized by RAW 264.7 macrophages and reduces the activity of the tumor-associated protease legumain Bioact Carbohydr Dietary Fibre (2015) 6, 15-23. DOI
Berven,L., Karppinen,P., Hetland,G. and Samuelsen,A.B. The polar high molecular weight fraction of the Agaricus blazei Murill extract, Andosan, reduces the activity of the tumor-associated protease, legumain, in RAW 264.7 cells J Med Food (2015) 18, 429-438. PubMed Europe PubMed DOI I
Brahimi-Horn,M.C., Lacas-Gervais,S., Adaixo,R., Ilc,K., Rouleau,M., Notte,A., Dieu,M., Michiels,C., Voeltzel,T., Maguer-Satta,V., Pelletier,J., Ilie,M., Hofman,P., Manoury,B., Schmidt,A., Hiller,S., Pouyssegur,J. and Mazure,N.M. Local mitochondrial-endolysosomal microfusion cleaves voltage-dependent anion channel 1 to promote survival in hypoxia Mol Cell Biol (2015) 35, 1491-1505. PubMed Europe PubMed DOI K
Brix,K., McInnes,J., Al-Hashimi,A., Rehders,M., Tamhane,T. and Haugen,M.H. Proteolysis mediated by cysteine cathepsins and legumain - recent advances and cell biological challenges Protoplasma (2015) 252, 755-774. PubMed Europe PubMed DOI
Chen,H., Liu,X., Clayman,E.S., Shao,F., Xiao,M., Tian,X., Fu,W., Zhang,C., Ruan,B., Zhou,P., Liu,Z., Wang,Y. and Rui,W. Synthesis and Evaluation of a CBZ-AAN-Dox Prodrug and its in vitro Effects on SiHa Cervical Cancer Cells under Hypoxic Conditions Chem Biol Drug Des (2015) 86, 589-598. PubMed Europe PubMed DOI
Dall,E., Fegg,J.C., Briza,P. and Brandstetter,H. Structure and mechanism of an aspartimide-dependent peptide ligase in human legumain Angew Chem Int Ed Engl (2015) 54, 2917-2921. PubMed Europe PubMed DOI S
Edgington-Mitchell,L.E., Rautela,J., Duivenvoorden,H.M., Jayatilleke,K.M., van der Linden,W.A., Verdoes,M., Bogyo,M. and Parker,B.S. Cysteine cathepsin activity suppresses osteoclastogenesis of myeloid-derived suppressor cells in breast cancer Oncotarget (2015) 6, 27008-27022. PubMed Europe PubMed DOI
Haugen,M.H., Boye,K., Nesland,J.M., Pettersen,S.J., Egeland,E.V., Tamhane,T., Brix,K., Maelandsmo,G.M. and Flatmark,K. High expression of the cysteine proteinase legumain in colorectal cancer - implications for therapeutic targeting Eur J Cancer (2015) 51, 9-17. PubMed Europe PubMed DOI T
Lin,S., Deng,F., Huang,P., Li,L., Wang,L., Li,Q., Chen,L., Chen,H. and Nan,K. A novel legumain protease-activated micelle cargo enhances anticancer activity and cellular internalization of doxorubicin J Mater Chem B (2015) 3, 6001-6012. DOI
Liu,Y., Goswami,R.K., Liu,C. and Sinha,S.C. Chemically programmed bispecific antibody targeting legumain protease and alphavbeta3 integrin mediates strong antitumor effects Mol Pharm (2015) 12, 2544-2550. PubMed Europe PubMed DOI
Ness,K.A., Eddie,S.L., Higgins,C.A., Templeman,A., D'Costa,Z., Gaddale,K.K., Bouzzaoui,S., Jordan,L., Janssen,D., Harrison,T., Burkamp,F., Young,A., Burden,R., Scott,C.J., Mullan,P.B. and Williams,R. Development of a potent and selective cell penetrant legumain inhibitor Bioorg Med Chem Lett (2015) 25, 5642-5645. PubMed Europe PubMed DOI I
Satoh,J., Asahina,N., Kitano,S. and Kino,Y. Legumain is expressed on macrophages in active demyelinating lesions of multiple sclerosis Clin Exp Neuroimmunol (2015) 6, 304-305. DOI
Solberg,R., Smith,R., Almlof,M., Tewolde,E., Nilsen,H. and Johansen,H.T. Legumain expression, activity and secretion are increased during monocyte-to-macrophage differentiation and inhibited by atorvastatin Biol Chem (2015) 396, 71-80. PubMed Europe PubMed DOI I
Yamane,T., Yamamoto,Y., Nakano,Y., Nakagaki,T., Ohkubo,I. and Ariga,H. Expression and protease activity of mouse legumain are regulated by the oncogene/transcription co-activator, DJ-1 through p53 and cleavage of annexin A2 is increased in DJ-1-knockout cells Biochem Biophys Res Commun (2015) 467, 472-477. PubMed Europe PubMed DOI
Yuan,Y., Ge,S., Sun,H., Dong,X., Zhao,H., An,L., Zhang,J., Wang,J., Hu,B. and Liang,G. Intracellular self-assembly and disassembly of (19)F nanoparticles confer respective "off" and "on" (19)F NMR/MRI signals for legumain activity detection in zebrafish ACS Nano (2015) 9, 5117-5124. PubMed Europe PubMed DOI L
Zhang,Z., Song,M., Liu,X., Su Kang,S., Duong,D.M., Seyfried,N.T., Cao,X., Cheng,L., Sun,Y.E., Ping Yu,S., Jia,J., Levey,A.I. and Ye,K. Delta-secretase cleaves amyloid precursor protein and regulates the pathogenesis in Alzheimer's disease Nat Commun (2015) 6, 8762-8762. PubMed Europe PubMed DOI
Zhen,Y., Chunlei,G., Wenzhi,S., Shuangtao,Z., Na,L., Rongrong,W., Xiaohe,L., Haiying,N., Dehong,L., Shan,J., Xiaoyue,T. and Rong,X. Clinicopathologic significance of legumain overexpression in cancer: a systematic review and meta-analysis Sci Rep (2015) 5, 16599-16599. PubMed Europe PubMed DOI T

2014

Chen,Y.J., Wu,S.C., Chen,C.Y., Tzou,S.C., Cheng,T.L., Huang,Y.F., Yuan,S.S. and Wang,Y.M. Peptide-based MRI contrast agent and near-infrared fluorescent probe for intratumoral legumain detection Biomaterials (2014) 35, 304-315. PubMed Europe PubMed DOI L
D'Costa,Z.C., Higgins,C., Ong,C.W., Irwin,G.W., Boyle,D., McArt,D.G., McCloskey,K., Buckley,N.E., Crawford,N.T., Thiagarajan,L., Murray,J.T., Kennedy,R.D., Mulligan,K.A., Harkin,D.P., Waugh,D.J.J., Scott,C.J., Salto-Tellez,M., Williams,R. and Mullan,P.B. TBX2 represses CST6 resulting in uncontrolled legumain activity to sustain breast cancer proliferation: a novel cancer-selective target pathway with therapeutic opportunities Oncotarget (2014) 5, 1609-1620. PubMed Europe PubMed
Hasegawa,S., Yamasaki,M. and Fukui,T. Degradation of acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, by legumain in the mouse kidney Biochem Biophys Res Commun (2014) 453, 631-635. PubMed Europe PubMed DOI
Higgins,C., Bouazzaoui,S., Gaddale,K., D'Costa,Z., Templeman,A., O'Rourke,M., Young,A., Scott,C., Harrison,T., Mullan,P. and Williams,R. P3 SAR exploration of biphenyl carbamate based legumain inhibitors Bioorg Med Chem Lett (2014) 24, 2521-2524. PubMed Europe PubMed DOI I
Jiang,Y., Lu,J., Wang,Y., Zeng,F., Wang,H., Peng,H., Huang,M., Jiang,H., Luo,C. and Huang,Y. Molecular-dynamics-simulation-driven design of a protease-responsive probe for in-vivo tumor imaging Adv Mater (2014) 26, 8174-8178. PubMed Europe PubMed DOI L
Lin,Y., Qiu,Y., Xu,C., Liu,Q., Peng,B., Kaufmann,G.F., Chen,X., Lan,B., Wei,C., Lu,D., Zhang,Y., Guo,Y., Lu,Z., Jiang,B., Edgington,T.S. and Guo,F. Functional role of asparaginyl endopeptidase ubiquitination by TRAF6 in tumor invasion and metastasis J Natl Cancer Inst (2014) 106, dju012-dju012. PubMed Europe PubMed DOI
Liu,Z., Xiong,M., Gong,J., Zhang,Y., Bai,N., Luo,Y., Li,L., Wei,Y., Liu,Y., Tan,X. and Xiang,R. Legumain protease-activated TAT-liposome cargo for targeting tumours and their microenvironment Nat Commun (2014) 5, 4280-4280. PubMed Europe PubMed DOI
Ma,L., Shen,Y.Q., Khatri,H.P. and Schachner,M. The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish PLoS ONE (2014) 9, e95098-e95098. PubMed Europe PubMed DOI PMC EPMC
Rosenmann,H. Asparagine endopeptidase cleaves tau and promotes neurodegeneration Nat Med (2014) 20, 1236-1238. PubMed Europe PubMed DOI
Smahel,M., Duskova,M., Polakova,I. and Musil,J. Enhancement of DNA vaccine potency against legumain J Immunother (2014) 37, 293-303. PubMed Europe PubMed DOI
Smith,R., Solberg,R., Jacobsen,L.L., Voreland,A.L., Rustan,A.C., Thoresen,G.H. and Johansen,H.T. Simvastatin inhibits glucose metabolism and legumain activity in human myotubes PLoS ONE (2014) 9, e85721-e85721. PubMed Europe PubMed DOI PMC EPMC
Smith,R.L., Strand,O.A., Nguyen,L.M., Elvestrand,T., Hagelin,G., Solberg,R., Johansen,H.T. and Rongved,P. Synthesis of a novel legumain-cleavable colchicine prodrug with cell-specific toxicity Bioorg Med Chem (2014) 22, 3309-3315. PubMed Europe PubMed DOI
Stoddart,A. Protease probe Nat Mater (2014) 13, 1074-1074. DOI L
Tohme,M. and Manoury,B. Intracellular toll-like receptor recruitment and cleavage in endosomal/lysosomal organelles Methods Enzymol (2014) 535, 141-147. PubMed Europe PubMed DOI
Wang,Y., Jiang,Y., Zhang,M., Tan,J., Liang,J., Wang,H., Li,Y., He,H., Yang,V.C. and Huang,Y. Protease-activatable hybrid nanoprobe for tumor imaging Adv Funct Mater (2014) 24, 5443-5453. DOI L
Wu,M., Shao,G.R., Zhang,F.X., Wu,W.X., Xu,P. and Ruan,Z.M. Legumain protein as a potential predictive biomarker for asian patients with breast carcinoma Asian Pac J Cancer Prev (2014) 15, 10773-10777. PubMed Europe PubMed DOI
Zhang,Z., Song,M., Liu,X., Kang,S.S., Kwon,I.S., Duong,D.M., Seyfried,N.T., Hu,W.T., Liu,Z., Wang,J.Z., Cheng,L., Sun,Y.E., Yu,S.P., Levey,A.I. and Ye,K. Cleavage of tau by asparagine endopeptidase mediates the neurofibrillary pathology in Alzheimer's disease Nat Med (2014) 20, 1254-1262. PubMed Europe PubMed DOI K T
Zhao,L., Hua,T., Crowley,C., Ru,H., Ni,X., Shaw,N., Jiao,L., Ding,W., Qu,L., Hung,L.W., Huang,W., Liu,L., Ye,K., Ouyang,S., Cheng,G. and Liu,Z.J. Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage Cell Res (2014) 24, 344-358. PubMed Europe PubMed DOI S

2013

Basurto-Islas,G., Grundke-Iqbal,I., Tung,Y.C., Liu,F. and Iqbal,K. Activation of asparaginyl endopeptidase leads to tau hyperphosphorylation in Alzheimer disease J Biol Chem (2013) 288, 17495-17507. PubMed Europe PubMed DOI
Berven,L., Johansen,H.T., Solberg,R., Kolset,S.O. and Samuelsen,A.B. Autoactivation of prolegumain is accelerated by glycosaminoglycans Biochimie (2013) 95, 772-781. PubMed Europe PubMed DOI
Berven,L., Solberg,R., Truong,H.H.T., Arlov,O., Lillelund Aachmann,F., Skjak-Braek,G., Egge-Jacobsen,W.M., Thidemann Johansen,H. and Samuelsen,A.B.C. Alginates induce legumain activity in RAW 264.7 cells and accelerate autoactivation of prolegumain Bioact Carbohydr Dietary Fibre (2013) 2, 30-44. DOI
Brocklehurst,K. and Philpott,M.P. Cysteine proteases: mode of action and role in epidermal differentiation Cell Tissue Res (2013) 351, 237-244. PubMed Europe PubMed DOI
Dall,E. and Brandstetter,H. Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation Proc Natl Acad Sci U S A (2013) 110, 10940-10945. PubMed Europe PubMed DOI S
Edgington,L.E., Verdoes,M., Ortega,A., Withana,N.P., Lee,J., Syed,S., Bachmann,M.H., Blum,G. and Bogyo,M. Functional imaging of legumain in cancer using a new quenched activity-based probe J Am Chem Soc (2013) 135, 174-182. PubMed Europe PubMed DOI L
Guo,P., Zhu,Z., Sun,Z., Wang,Z., Zheng,X. and Xu,H. Expression of legumain correlates with prognosis and metastasis in gastric carcinoma PLoS ONE (2013) 8, e73090-e73090. PubMed Europe PubMed DOI PMC EPMC
Haugen,M.H., Johansen,H.T., Pettersen,S.J., Solberg,R., Brix,K., Flatmark,K. and Maelandsmo,G.M. Nuclear legumain activity in colorectal cancer PLoS ONE (2013) 8, e52980-e52980. PubMed Europe PubMed DOI PMC EPMC
Iqbal,K., Bolognin,S., Wang,X., Basurto-Islas,G., Blanchard,J. and Tung,Y.C. Animal models of the sporadic form of Alzheimer's disease: focus on the disease and not just the lesions J Alzheimers Dis (2013) 37, 469-474. PubMed Europe PubMed DOI
Li,N., Liu,Q., Su,Q., Wei,C., Lan,B., Wang,J., Bao,G., Yan,F., Yu,Y., Peng,B., Qiu,J., Yan,X., Zhang,S. and Guo,F. Effects of legumain as a potential prognostic factor on gastric cancers Med Oncol (2013) 30, 621-621. PubMed Europe PubMed DOI
Lin,Y., Wei,C., Liu,Y., Qiu,Y., Liu,C. and Guo,F. Selective ablation of tumor-associated macrophages suppresses metastasis and angiogenesis Cancer Sci (2013) 104, 1217-1225. PubMed Europe PubMed DOI
Liu,B., Gong,X., Chang,S., Sun,P. and Wu,J. Generation of mature Nalpha-terminal acetylated thymosin alpha1 by cleavage of recombinant prothymosin alpha ScientificWorldJournal (2013) 2013, 387282-387282. PubMed Europe PubMed DOI PMC EPMC
Liu,Z., Lv,D., Liu,S., Gong,J., Wang,D., Xiong,M., Chen,X., Xiang,R. and Tan,X. Alginic acid-coated chitosan nanoparticles loaded with legumain DNA vaccine: effect against breast cancer in mice PLoS ONE (2013) 8, e60190-e60190. PubMed Europe PubMed DOI PMC EPMC
Ohno,Y., Nakashima,J., Izumi,M., Ohori,M., Hashimoto,T. and Tachibana,M. Association of legumain expression pattern with prostate cancer invasiveness and aggressiveness World J Urol (2013) 31, 359-364. PubMed Europe PubMed DOI
Wallin,H., Abrahamson,M. and Ekstrom,U. Cystatin C properties crucial for uptake and inhibition of intracellular target enzymes J Biol Chem (2013) 288, 17019-17029. PubMed Europe PubMed DOI
Yamane,T., Hachisu,R., Yuguchi,M., Takeuchi,K., Murao,S., Yamamoto,Y., Ogita,H., Takasawa,T., Ohkubo,I. and Ariga,H. Knockdown of legumain inhibits cleavage of annexin A2 in the mouse kidney Biochem Biophys Res Commun (2013) 430, 482-487. PubMed Europe PubMed DOI K
Yamane,T., Murao,S., Kato-Ose,I., Kashima,L., Yuguchi,M., Kozuka,M., Takeuchi,K., Ogita,H., Ohkubo,I. and Ariga,H. Transcriptional regulation of the legumain gene by p53 in HCT116 cells Biochem Biophys Res Commun (2013) 438, 613-618. PubMed Europe PubMed DOI

2012

Barrett,A.J. and Chen,J.M. Animal legumain [ISSN:978-0-12-407743-0] (2012) 3, 2309-2314. DOI
Dall,E. and Brandstetter,H. Activation of legumain involves proteolytic and conformational events, resulting in a context- and substrate-dependent activity profile Acta Crystallogr Sect F Struct Biol Cryst Commun (2012) 68, 24-31. PubMed Europe PubMed DOI PMC EPMC
Descamps,D., Le Gars,M., Balloy,V., Barbier,D., Maschalidi,S., Tohme,M., Chignard,M., Ramphal,R., Manoury,B. and Sallenave,J.M. Toll-like receptor 5 (TLR5), IL-1beta secretion, and asparagine endopeptidase are critical factors for alveolar macrophage phagocytosis and bacterial killing Proc Natl Acad Sci U S A (2012) 109, 1619-1624. PubMed Europe PubMed DOI PMC EPMC
Herskowitz,J.H., Gozal,Y.M., Duong,D.M., Dammer,E.B., Gearing,M., Ye,K., Lah,J.J., Peng,J., Levey,A.I. and Seyfried,N.T. Asparaginyl endopeptidase cleaves TDP-43 in brain Proteomics (2012) 12, 2455-2463. PubMed Europe PubMed DOI
Lee,J. and Bogyo,M. Synthesis and evaluation of aza-peptidyl inhibitors of the lysosomal asparaginyl endopeptidase, legumain Bioorg Med Chem Lett (2012) 22, 1340-1343. PubMed Europe PubMed DOI I
Liu,Y., Bajjuri,K.M., Liu,C. and Sinha,S.C. Targeting cell surface alpha(v)beta(3) integrin increases therapeutic efficacies of a legumain protease-activated auristatin prodrug Mol Pharm (2012) 9, 168-175. PubMed Europe PubMed DOI
Maschalidi,S., Hassler,S., Blanc,F., Sepulveda,F.E., Tohme,M., Chignard,M., van Endert,P., Si-Tahar,M., Descamps,D. and Manoury,B. Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation PLoS Pathog (2012) 8, e1002841-e1002841. PubMed Europe PubMed DOI PMC EPMC
Smith,R., Johansen,H.T., Nilsen,H., Haugen,M.H., Pettersen,S.J., Maelandsmo,G.M., Abrahamson,M. and Solberg,R. Intra- and extracellular regulation of activity and processing of legumain by cystatin E/M Biochimie (2012) 94, 2590-2599. PubMed Europe PubMed DOI
Wang,L., Chen,S., Zhang,M., Li,N., Chen,Y., Su,W., Liu,Y., Lu,D., Li,S., Yang,Y., Li,Z., Stupack,D., Qu,P., Hu,H. and Xiang,R. Legumain: a biomarker for diagnosis and prognosis of human ovarian cancer J Cell Biochem (2012) 113, 2679-2686. PubMed Europe PubMed DOI

2011

Andrade,V., Guerra,M., Jardim,C., Melo,F., Silva,W., Ortega,J.M., Robert,M., Nathanson,M.H. and Leite,F. Nucleoplasmic calcium regulates cell proliferation through legumain J Hepatol (2011) 55, 626-635. PubMed Europe PubMed DOI K
Bajjuri,K.M., Liu,Y., Liu,C. and Sinha,S.C. The legumain protease-activated auristatin prodrugs suppress tumor growth and metastasis without toxicity ChemMedChem (2011) 6, 54-59. PubMed Europe PubMed DOI
Liao,D., Liu,Z., Wrasidlo,W., Chen,T., Luo,Y., Xiang,R. and Reisfeld,R.A. Synthetic enzyme inhibitor: a novel targeting ligand for nanotherapeutic drug delivery inhibiting tumor growth without systemic toxicity Nanomedicine (2011) 7, 665-673. PubMed Europe PubMed DOI I
Miller,G., Matthews,S.P., Reinheckel,T., Fleming,S. and Watts,C. Asparagine endopeptidase is required for normal kidney physiology and homeostasis FASEB J (2011) 25, 1606-1617. PubMed Europe PubMed DOI K
Overbye,A., Saetre,F., Hagen,L.K., Johansen,H.T. and Seglen,P.O. Autophagic activity measured in whole rat hepatocytes as the accumulation of a novel BHMT fragment (p10), generated in amphisomes by the asparaginyl proteinase, legumain Autophagy (2011) 7, 1011-1027. PubMed Europe PubMed DOI I
Stickler,M.M., Reddy,A., Xiong,J.M., Hinton,P.R., Dubridge,R. and Harding,F.A. The human G1m1 allotype associates with CD4+ T-cell responsiveness to a highly conserved IgG1 constant region peptide and confers an asparaginyl endopeptidase cleavage site Genes Immun (2011) 12, 213-221. PubMed Europe PubMed DOI

2010

Briggs,J.J., Haugen,M.H., Johansen,H.T., Riker,A.I., Abrahamson,M., Fodstad,O., Maelandsmo,G.M. and Solberg,R. Cystatin E/M suppresses legumain activity and invasion of human melanoma BMC Cancer (2010) 10, 17-17. PubMed Europe PubMed DOI
Ishizaki,T., Erickson,A., Kuric,E., Shamloo,M., Hara-Nishimura,I., Inacio,A.R., Wieloch,T. and Ruscher,K. The asparaginyl endopeptidase legumain after experimental stroke J Cereb Blood Flow Metab (2010) 30, 1756-1766. PubMed Europe PubMed DOI K
Lee,J. and Bogyo,M. Development of near-infrared fluorophore (NIRF)-labeled activity-based probes for in vivo imaging of legumain ACS Chem Biol (2010) 5, 233-243. PubMed Europe PubMed DOI L I
Matthews,S.P., Werber,I., Deussing,J., Peters,C., Reinheckel,T. and Watts,C. Distinct protease requirements for antigen presentation in vitro and in vivo J Immunol (2010) 184, 2423-2431. PubMed Europe PubMed DOI K
Mattock,K.L., Gough,P.J., Humphries,J., Burnand,K., Patel,L., Suckling,K.E., Cuello,F., Watts,C., Gautel,M., Avkiran,M. and Smith,A. Legumain and cathepsin-L expression in human unstable carotid plaque Atherosclerosis (2010) 208, 83-89. PubMed Europe PubMed DOI
Ren,Q., Zhang,X.W., Sun,Y.D., Sun,S.S., Zhou,J., Wang,Z.H., Zhao,X.F. and Wang,J.X. Two cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis Fish Shellfish Immunol (2010) 29, 551-556. PubMed Europe PubMed DOI
Teng,L., Wada,H. and Zhang,S. Identification and functional characterization of legumain in amphioxus Branchiostoma belcheri Biosci Rep (2010) 30, 177-186. PubMed Europe PubMed DOI

2009

Alim,M.A., Tsuji,N., Miyoshi,T., Islam,M.K., Hatta,T. and Fujisaki,K. Legumains from the hard tick Haemaphysalis longicornis play modulatory roles in blood feeding and gut cellular remodelling and impact on embryogenesis Int J Parasitol (2009) 39, 97-107. PubMed Europe PubMed DOI
Andrade,V.A., Jardim,C.A., Melo,F.M., Silva,W.A., Nathanson,M.H., Ortega,J.M. and Leite,M.F. Nucleoplasmic calcium regulates proliferation of hepatocytes through legumain and reticulon4 Gastroenterology (2009) 136, A792-A792.
Chan,C.B., Abe,M., Hashimoto,N., Hao,C., Williams,I.R., Liu,X., Nakao,S., Yamamoto,A., Zheng,C., Henter,J.I., Meeths,M., Nordenskjold,M., Li,S.Y., Hara-Nishimura,I., Asano,M. and Ye,K. Mice lacking asparaginyl endopeptidase develop disorders resembling hemophagocytic syndrome Proc Natl Acad Sci U S A (2009) 106, 468-473. PubMed Europe PubMed DOI PMC EPMC K
Ledgard,A.M., Lee,R.S. and Peterson,A.J. Bovine endometrial legumain and TIMP-2 regulation in response to presence of a conceptus Mol Reprod Dev (2009) 76, 65-74. PubMed Europe PubMed DOI
Ovat,A., Muindi,F., Fagan,C., Brouner,M., Hansell,E., Dvorak,J., Sojka,D., Kopacek,P., McKerrow,J.H., Caffrey,C.R. and Powers,J.C. Aza-peptidyl Michael acceptor and epoxide inhibitors - potent and selective inhibitors of Schistosoma mansoni and Ixodes ricinus legumains (asparaginyl endopeptidases) J Med Chem (2009) 52, 7192-7210. PubMed Europe PubMed DOI I
Patel,N., Krishnan,S., Offman,M.N., Krol,M., Moss,C.X., Leighton,C., van Delft,F.W., Holland,M., Liu,J., Alexander,S., Dempsey,C., Ariffin,H., Essink,M., Eden,T.O., Watts,C., Bates,P.A. and Saha,V. A dyad of lymphoblastic lysosomal cysteine proteases degrades the antileukemic drug l-asparaginase J Clin Invest (2009) 119, 1964-1973. PubMed Europe PubMed DOI PMC EPMC P
Sepulveda,F.E., Maschalidi,S., Colisson,R., Heslop,L., Ghirelli,C., Sakka,E., Lennon-Dumenil,A.M., Amigorena,S., Cabanie,L. and Manoury,B. Critical role for asparagine endopeptidase in endocytic Toll-like receptor signaling in dendritic cells Immunity (2009) 31, 737-748. PubMed Europe PubMed DOI K
Stern,L., Perry,R., Ofek,P., Many,A., Shabat,D. and Satchi-Fainaro,R. A novel antitumor prodrug platform designed to be cleaved by the endoprotease legumain Bioconjug Chem (2009) 20, 500-510. PubMed Europe PubMed DOI
van Endert,P. Toll-like receptor 9: AEP takes control Immunity (2009) 31, 696-698. PubMed Europe PubMed DOI

2008

Alim,M.A., Tsuji,N., Miyoshi,T., Islam,M.K., Huang,X., Hatta,T. and Fujisaki,K. HlLgm2, a member of asparaginyl endopeptidases/legumains in the midgut of the ixodid tick Haemaphysalis longicornis, is involved in blood-meal digestion J Insect Physiol (2008) 54, 573-585. PubMed Europe PubMed DOI
Alim,M.A., Tsuji,N., Miyoshi,T., Islam,M.K., Hatta,T., Yamaji,K. and Fujisaki,K. Developmental stage- and organ-specific expression profiles of asparaginyl endopeptidases/legumains in the ixodid tick Haemaphysalis longicornis J Vet Med Sci (2008) 70, 1363-1366. PubMed Europe PubMed DOI
Clerin,V., Shih,H.H., Deng,N., Hebert,G., Resmini,C., Shields,K.M., Feldman,J.L., Winkler,A., Albert,L., Maganti,V., Wong,A., Paulsen,J.E., Keith,J.C., Jr., Vlasuk,G.P. and Pittman,D.D. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis Atherosclerosis (2008) 201, 53-66. PubMed Europe PubMed DOI
Costantino,C.M., Hang,H.C., Kent,S.C., Hafler,D.A. and Ploegh,H.L. Lysosomal cysteine and aspartic proteases are heterogeneously expressed and act redundantly to initiate human invariant chain degradation J Immunol (2008) 180, 2876-2885. PubMed Europe PubMed
Lewen,S., Zhou,H., Hu,H.D., Cheng,T., Markowitz,D., Reisfeld,R.A., Xiang,R. and Luo,Y. A legumain-based minigene vaccine targets the tumor stroma and suppresses breast cancer growth and angiogenesis Cancer Immunol Immunother (2008) 57, 507-515. PubMed Europe PubMed DOI
Liu,Z., Jang,S.W., Liu,X., Cheng,D., Peng,J., Yepes,M., Li,X.J., Matthews,S., Watts,C., Asano,M., Hara-Nishimura,I., Luo,H.R. and Ye,K. Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase Mol Cell (2008) 29, 665-678. PubMed Europe PubMed DOI
Sojka,D., Franta,Z., Horn,M., Hajdusek,O., Caffrey,C.R., Mares,M. and Kopacek,P. Profiling of proteolytic enzymes in the gut of the tick Ixodes ricinus reveals an evolutionarily conserved network of aspartic and cysteine peptidases Parasit Vectors (2008) 1, 7-7. PubMed Europe PubMed DOI PMC EPMC
Woehler,A., Fest,S., Stermann,A., Gaedicke,G., Lode,H.N. and Huebener,N. A novel legumain DNA vaccine effectively eradicates neuroblastoma and induces long-term survival in a preclinical neuroblastoma mouse model Acta Paediatr (2008) 97, 236-236.
Xiang,R., Luo,Y., Niethammer,A.G. and Reisfeld,R.A. Oral DNA vaccines target the tumor vasculature and microenvironment and suppress tumor growth and metastasis Immunol Rev (2008) 222, 117-128. PubMed Europe PubMed DOI

2007

Abdul Alim,M., Tsuji,N., Miyoshi,T., Khyrul Islam,M., Huang,X., Motobu,M. and Fujisaki,K. Characterization of asparaginyl endopeptidase, legumain induced by blood feeding in the ixodid tick Haemaphysalis longicornis Insect Biochem Mol Biol (2007) 37, 911-922. PubMed Europe PubMed DOI
Gawenda,J., Traub,F., Luck,H.J., Kreipe,H. and von Wasielewski,R. Legumain expression as a prognostic factor in breast cancer patients Breast Cancer Res Treat (2007) 102, 1-6. PubMed Europe PubMed DOI
Martinez,M., Diaz-Mendoza,M., Carrillo,L. and Diaz,I. Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinases FEBS Lett (2007) 581, 2914-2918. PubMed Europe PubMed DOI
Morita,Y., Araki,H., Sugimoto,T., Takeuchi,K., Yamane,T., Maeda,T., Yamamoto,Y., Nishi,K., Asano,M., Shirahama-Noda,K., Nishimura,M., Uzu,T., Hara-Nishimura,I., Koya,D., Kashiwagi,A. and Ohkubo,I. Legumain/asparaginyl endopeptidase controls extracellular matrix remodeling through the degradation of fibronectin in mouse renal proximal tubular cells FEBS Lett (2007) 581, 1417-1424. PubMed Europe PubMed DOI K
Sexton,K.B., Witte,M.D., Blum,G. and Bogyo,M. Design of cell-permeable, fluorescent activity-based probes for the lysosomal cysteine protease asparaginyl endopeptidase (AEP)/legumain Bioorg Med Chem Lett (2007) 17, 649-653. PubMed Europe PubMed DOI PMC EPMC L
Sojka,D., Hajdusek,O., Dvorak,J., Sajid,M., Franta,Z., Schneider,E.L., Craik,C.S., Vancova,M., Buresova,V., Bogyo,M., Sexton,K.B., McKerrow,J.H., Caffrey,C.R. and Kopacek,P. IrAE: an asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus Int J Parasitol (2007) 37, 713-724. PubMed Europe PubMed DOI PMC EPMC

2006

[YEAR:24-3-2006]Cheng,T., Hitomi,K., van Vlijmen-Willems,I.M., de Jongh,G.J., Yamamoto,K., Nishi,K., Watts,C., Reinheckel,T., Schalkwijk,J. and Zeeuwen,P.L. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification J Biol Chem (24-3-2006) 281, 15893-15899. PubMed Europe PubMed DOI
Davies,S., Dai,D.H., Pickett,G. and Leslie,K.K. Gene regulation profiles by progesterone and dexamethasone in human endometrial cancer Ishikawa H cells Gynecol Oncol (2006) 101, 62-70. PubMed Europe PubMed DOI
[YEAR:20-7-2006]Luo,Y., Zhou,H., Krueger,J., Kaplan,C., Lee,S.H., Dolman,C., Markowitz,D., Wu,W., Liu,C., Reisfeld,R.A. and Xiang,R. Targeting tumor-associated macrophages as a novel strategy against breast cancer J Clin Invest (20-7-2006) 116, 2132-2141. PubMed Europe PubMed DOI PMC EPMC
[YEAR:2-5-2006]Oliver,E.M., Skuce,P.J., McNair,C.M. and Knox,D.P. Identification and characterization of an asparaginyl proteinase (legumain) from the parasitic nematode, Haemonchus contortus Parasitology (2-5-2006) 133, 237-244. PubMed Europe PubMed DOI
Papaspyridonos,M., Smith,A., Burnand,K.G., Taylor,P., Padayachee,S., Suckling,K.E., James,C.H., Greaves,D.R. and Patel,L. Novel candidate genes in unstable areas of human atherosclerotic plaques Arterioscler Thromb Vasc Biol (2006) 26, 1837-1844. PubMed Europe PubMed DOI
Rawadi,G., Boulukos,K., Garcia,T., Pognonec,P., Martin,P., Touitou,R. and Roman,S. C-terminal fragment of legumain (CTF) modulates Wnt signaling and osteoblast mineralization J Bone Miner Res (2006) 21, S158-S158.
Wu,W., Luo,Y., Sun,C., Liu,Y., Kuo,P., Varga,J., Xiang,R., Reisfeld,R., Janda,K.D., Edgington,T.S. and Liu,C. Targeting cell-impermeable prodrug activation to tumor microenvironment eradicates multiple drug-resistant neoplasms Cancer Res (2006) 66, 970-980. PubMed Europe PubMed DOI

2005

[YEAR:1-6-2005]Maehr,R., Hang,H.C., Mintern,J.D., Kim,Y.M., Cuvillier,A., Nishimura,M., Yamada,K., Shirahama-Noda,K., Hara-Nishimura,I. and Ploegh,H.L. Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice J Immunol (1-6-2005) 174, 7066-7074. PubMed Europe PubMed K
[YEAR:6-5-2005]Moss,C.X., Matthews,S.P., Lamont,D.J. and Watts,C. Asparagine deamidation perturbs antigen presentation on class II major histocompatibility complex molecules J Biol Chem (6-5-2005) 280, 18498-18503. PubMed Europe PubMed DOI
Murray,J., Manoury,B., Balic,A., Watts,C. and Maizels,R.M. Bm-CPI-2, a cystatin from Brugia malayi nematode parasites, differs from Caenorhabditis elegans cystatins in a specific site mediating inhibition of the antigen-processing enzyme AEP Mol Biochem Parasitol (2005) 139, 197-203. PubMed Europe PubMed DOI
[YEAR:15-3-2005]Murthy,R.V., Arbman,G., Gao,J., Roodman,G.D. and Sun,X.F. Legumain expression in relation to clinicopathologic and biological variables in colorectal cancer Clin Cancer Res (15-3-2005) 11, 2293-2299. PubMed Europe PubMed DOI
Watts,C., Matthews,S.P., Mazzeo,D., Manoury,B. and Moss,C.X. Asparaginyl endopeptidase: case history of a class II MHC compartment protease Immunol Rev (2005) 207, 218-228. PubMed Europe PubMed DOI
[YEAR:5-5-2005]Wolk,K., Grutz,G., Witte,K., Volk,H.D. and Sabat,R. The expression of legumain, an asparaginyl endopeptidase that controls antigen processing, is reduced in endotoxin-tolerant monocytes Genes Immun (5-5-2005) 6, 452-456. PubMed Europe PubMed DOI

2004

[YEAR:1-5-2004]Burster,T., Beck,A., Tolosa,E., Marin-Esteban,V., Rotzschke,O., Falk,K., Lautwein,A., Reich,M., Brandenburg,J., Schwarz,G., Wiendl,H., Melms,A., Lehmann,R., Stevanovic,S., Kalbacher,H. and Driessen,C. Cathepsin G, and not the asparagine-specific endoprotease, controls the processing of myelin basic protein in lysosomes from human B lymphocytes J Immunol (1-5-2004) 172, 5495-5503. PubMed Europe PubMed
Chen,J.M., Dando,P.M. and Barrett,A.J. Mammalian legumain [ISSN:0-12-079610-4] (2004) 2, 1302-1305. V
[YEAR:8-4-2004]Ekici,O.D., Gotz,M.G., James,K.E., Li,Z.Z., Rukamp,B.J., Asgian,J.L., Caffrey,C.R., Hansell,E., Dvorak,J., McKerrow,J.H., Potempa,J., Travis,J., Mikolajczyk,J., Salvesen,G.S. and Powers,J.C. Aza-peptide Michael acceptors: a new class of inhibitors specific for caspases and other clan CD cysteine proteases J Med Chem (8-4-2004) 47, 1889-1892. PubMed Europe PubMed DOI I
Lecaille,F., Muno,D., Kominami,E. and Ishidoh,K. Proteinases participating in the processing and activation of prolegumain in primary cultured rat macrophages Biol Chem (2004) 385, 511-516. PubMed Europe PubMed DOI
[YEAR:15-5-2004]Zeeuwen,P.L., van Vlijmen-Willems,I.M., Olthuis,D., Johansen,H.T., Hitomi,K., Hara-Nishimura,I., Powers,J.C., James,K.E., Op den Camp,H.J., Lemmens,R. and Schalkwijk,J. Evidence that unrestricted legumain activity is involved in disturbed epidermal cornification in cystatin M/E deficient mice Hum Mol Genet (15-5-2004) 13, 1069-1079. PubMed Europe PubMed DOI

2003

James,K.E., Gotz,M.G., Caffrey,C.R., Hansell,E., Carter,W., Barrett,A.J., McKerrow,J.H. and Powers,J.C. Aza-peptide epoxides: potent and selective inhibitors of Schistosoma mansoni and pig kidney legumains (asparaginyl endopeptidases) Biol Chem (2003) 384, 1613-1618. PubMed Europe PubMed DOI I
[YEAR:3-10-2003]Li,D.N., Matthews,S.P., Antoniou,A.N., Mazzeo,D. and Watts,C. Multistep autoactivation of asparaginyl endopeptidase in vitro and in vivo J Biol Chem (3-10-2003) 278, 38980-38990. PubMed Europe PubMed DOI
[YEAR:1-6-2003]Liu,C., Sun,C., Huang,H., Janda,K. and Edgington,T. Overexpression of legumain in tumors is significant for invasion/metastasis and a candidate enzymatic target for prodrug therapy Cancer Res (1-6-2003) 63, 2957-2964. PubMed Europe PubMed
Loak,K., Li,D.N., Manoury,B., Billson,J., Morton,F., Hewitt,E. and Watts,C. Novel cell-permeable acyloxymethylketone inhibitors of asparaginyl endopeptidase Biol Chem (2003) 384, 1239-1246. PubMed Europe PubMed DOI I
Manoury,B., Mazzeo,D., Li,D.N., Billson,J., Loak,K., Benaroch,P. and Watts,C. Asparagine endopeptidase can initiate the removal of the MHC class II invariant chain chaperone Immunity (2003) 18, 489-498. PubMed Europe PubMed DOI
[YEAR:11-4-2003]Sarandeses,C.S., Covelo,G., Diaz-Jullien,C. and Freire,M. Prothymosin alpha is processed to thymosin alpha1 and thymosin alpha11 by a lysosomal asparaginyl endopeptidase J Biol Chem (11-4-2003) 278, 13286-13293. PubMed Europe PubMed DOI P
[YEAR:29-8-2003]Shirahama-Noda,K., Yamamoto,A., Sugihara,K., Hashimoto,N., Asano,M., Nishimura,M. and Hara-Nishimura,I. Biosynthetic processing of cathepsins and lysosomal degradation are abolished in asparaginyl endopeptidase-deficient mice J Biol Chem (29-8-2003) 278, 33194-33199. PubMed Europe PubMed DOI K
Watts,C., Moss,C.X., Mazzeo,D., West,M.A., Matthews,S.P., Li,D.N. and Manoury,B. Creation versus destruction of T cell epitopes in the class II MHC pathway Ann N Y Acad Sci (2003) 987, 9-14. PubMed Europe PubMed
Watts,C., Mazzeo,D., West,M.A., Matthews,S.P., Keane,D., Hamilton,G., Persson,L.V., Lawson,J.M., Manoury,B. and Moss,C.X. Roles for asparagine endopeptidase in class II MHC-restricted antigen processing Biochem Soc Symp (2003) 70, 31-38. PubMed Europe PubMed

2002

[YEAR:7-11-2002]Asgian,J.L., James,K.E., Li,Z.Z., Carter,W., Barrett,A.J., Mikolajczyk,J., Salvesen,G.S. and Powers,J.C. Aza-peptide epoxides: a new class of inhibitors selective for clan CD cysteine proteases J Med Chem (7-11-2002) 45, 4958-4960. PubMed Europe PubMed DOI I
[YEAR:30-4-2002]Mathieu,M.A., Bogyo,M., Caffrey,C.R., Choe,Y., Lee,J., Chapman,H., Sajid,M., Craik,C.S. and McKerrow,J.H. Substrate specificity of schistosome versus human legumain determined by P1-P3 peptide libraries Mol Biochem Parasitol (30-4-2002) 121, 99-105. PubMed Europe PubMed DOI P
Niestroj,A.J., Feussner,K., Heiser,U., Dando,P.M., Barrett,A., Gerhartz,B. and Demuth,H.U. Inhibition of mammalian legumain by Michael acceptors and AzaAsn-halomethylketones Biol Chem (2002) 383, 1205-1214. PubMed Europe PubMed DOI I
Schwarz,G., Brandenburg,J., Reich,M., Burster,T., Driessen,C. and Kalbacher,H. Characterization of legumain Biol Chem (2002) 383, 1813-1816. PubMed Europe PubMed DOI
[YEAR:1-4-2002]Yamane,T., Takeuchi,K., Yamamoto,Y., Li,Y.H., Fujiwara,M., Nishi,K., Takahashi,S. and Ohkubo,I. Legumain from bovine kidney: its purification, molecular cloning, immunohistochemical localization and degradation of annexin II and vitamin D-binding protein Biochim Biophys Acta (1-4-2002) 1596, 108-120. PubMed Europe PubMed DOI

2001

Beck,H., Schwarz,G., Schroter,C.J., Deeg,M., Baier,D., Stevanovic,S., Weber,E., Driessen,C. and Kalbacher,H. Cathepsin S and an asparagine-specific endoprotease dominate the proteolytic processing of human myelin basic protein in vitro Eur J Immunol (2001) 31, 3726-3736. PubMed Europe PubMed DOI
Chen,J.M., Fortunato,M., Stevens,R.A.E. and Barrett,A.J. Activation of progelatinase A by mammalian legumain, a recently discovered cysteine proteinase Biol Chem (2001) 382, 777-783. PubMed Europe PubMed DOI
Choi,S.J., Kurihara,N., Oba,Y. and Roodman,G.D. Osteoclast inhibitory peptide 2 inhibits osteoclast formation via its C-terminal fragment J Bone Miner Res (2001) 16, 1804-1811. PubMed Europe PubMed DOI
Rotari,V.I., Dando,P.M. and Barrett,A.J. Legumain forms from plants and animals differ in their specificity Biol Chem (2001) 382, 953-959. PubMed Europe PubMed DOI
[YEAR:15-7-2001]Snipas,S.J., Stennicke,H.R., Riedl,S., Potempa,J., Travis,J., Barrett,A.J. and Salvesen,G.S. Inhibition of distant caspase homologues by natural caspase inhibitors Biochem J (15-7-2001) 357, 575-580. PubMed Europe PubMed DOI PMC EPMC

2000

[YEAR:1-12-2000]Chen,J.M., Fortunato,M. and Barrett,A.J. Activation of human prolegumain by cleavage at a C-terminal asparagine residue Biochem J (1-12-2000) 352, 327-334. PubMed Europe PubMed DOI PMC EPMC

1999

[YEAR:2-7-1999]Alvarez-Fernandez,M., Barrett,A.J., Gerhartz,B., Dando,P.M., Ni,J. and Abrahamson,M. Inhibition of mammalian legumain by some cystatins is due to a novel second reactive site J Biol Chem (2-7-1999) 274, 19195-19203. PubMed Europe PubMed DOI
[YEAR:1-5-1999]Dando,P.M., Fortunato,M., Smith,L., Knight,C.G., McKendrick,J.E. and Barrett,A.J. Pig kidney legumain: an asparaginyl endopeptidase with restricted specificity Biochem J (1-5-1999) 339, 743-749. PubMed Europe PubMed DOI PMC EPMC
[YEAR:10-9-1999]Johansen,H.T., Knight,C.G. and Barrett,A.J. Colorimetric and fluorimetric microplate assays for legumain and a staining reaction for detection of the enzyme after electrophoresis Anal Biochem (10-9-1999) 273, 278-283. PubMed Europe PubMed DOI A

1998

[YEAR:17-12-1998]Bogyo,M. and Ploegh,H.L. Antigen presentation - a protease draws first blood Nature (17-12-1998) 396, 625-627. PubMed Europe PubMed DOI
[YEAR:28-12-1998]Chen,J.M., Rawlings,N.D., Stevens,R.A. and Barrett,A.J. Identification of the active site of legumain links it to caspases, clostripain and gingipains in a new clan of cysteine endopeptidases FEBS Lett (28-12-1998) 441, 361-365. PubMed Europe PubMed DOI
[YEAR:1-10-1998]Chen,J.M., Dando,P.M., Stevens,R.A., Fortunato,M. and Barrett,A.J. Cloning and expression of mouse legumain, a lysosomal endopeptidase Biochem J (1-10-1998) 335, 111-117. PubMed Europe PubMed PMC EPMC
Halfon,S., Patel,S., Vega,F., Zurawski,S. and Zurawski,G. Autocatalytic activation of human legumain at aspartic acid residues FEBS Lett (1998) 438, 114-118. PubMed Europe PubMed DOI E P
[YEAR:17-12-1998]Manoury,B., Hewitt,E.W., Morrice,N., Dando,P.M., Barrett,A.J. and Watts,C. An asparaginyl endopeptidase processes a microbial antigen for class II MHC presentation Nature (17-12-1998) 396, 695-699. PubMed Europe PubMed DOI

1997

[YEAR:21-3-1997]Chen,J.M., Dando,P.M., Rawlings,N.D., Brown,M.A., Young,N.E., Stevens,R.A., Hewitt,E., Watts,C. and Barrett,A.J. Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase J Biol Chem (21-3-1997) 272, 8090-8098. PubMed Europe PubMed DOI I

1996

Tanaka,T., Inazawa,J. and Nakamura,Y. Molecular cloning of a human cDNA encoding putative cysteine protease (PRSC1) and its chromosome assignment to 14q32.1 Cytogenet Cell Genet (1996) 74, 120-123. PubMed Europe PubMed