Structure analysis

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a Valine-ASL with cmo5U in position 34 bound to an mRNA with a GUA-codon in the A-site and paromomycin.

X-ray diffraction
3.1Å resolution
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero 23-mer (preferred)
Entry contents: 20 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 23-mer
Accessible surface area: 272851.28 Å2
Buried surface area: 128367.59 Å2
Dissociation area: 614.19 Å2
Dissociation energy (ΔGdiss): -5.95 kcal/mol
Dissociation entropy (TΔSdiss): 10.1 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-215895

Macromolecules

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Chain: L
Length: 135 amino acids
Theoretical weight: 14.92 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:
CATH: Nucleic acid-binding proteins
SCOP: Cold shock DNA-binding domain-like

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Chain: O
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:
CATH: S15/NS1, RNA-binding
SCOP: Ribosomal protein S15

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Chain: P
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:
CATH: S16 Ribosomal Protein; Chain: A;
SCOP: Ribosomal protein S16

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Chain: R
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:
CATH: Ribosomal protein S18
SCOP: Ribosomal protein S18

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Chain: T
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:
CATH: Ribosomal protein S20
SCOP: Ribosomal protein S20

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Name: 16S ribosomal RNA
Representative chains: A
Length: 1522 nucleotides
Theoretical weight: 493.96 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: 5'-R(*GP*UP*AP*AP*AP*AP)-3'
Representative chains: X
Length: 6 nucleotides
Theoretical weight: 1.92 KDa

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Name: 5'-R(*CP*CP*UP*CP*CP*CP*UP*CM0P*AP*CP*6MZP*AP *GP*GP*AP*GP*G)-3'
Representative chains: Y
Length: 17 nucleotides
Theoretical weight: 5.49 KDa

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