Substrates for peptidase C54.001: ATG4 peptidase

Summary Alignment Tree Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate Uniprot Residue range Cleavage Site Cleavage type Evidence P4 P3 P2 P1 P1' P2' P3' P4' Reference CutDB MERNUM
Ac-Tyr-Ser-Gly-Glu-Asn-Thr-Phe-Gly-NHMec Ac-Tyr-Ser-Gly-Glu-Asn-Thr-Phe-Gly+NHMec S Asn Thr Phe Gly AMC - - - Kirisako & Ohsumi, 2004
ATG4 peptidase P53867 1-494 peptide-Cys159+Met-peptide P Gly Trp Gly Cys Met Ile Arg Thr Kirisako & Ohsumi, 2004 16379
autophagy-related protein 8 P38182 1-117 peptide-Gly116+Arg P Asn Thr Phe Gly Arg - - - Kirisako & Ohsumi, 2004 16380
Boc-Thr-Phe-Gly-NHMec Boc-Thr-Phe-Gly+NHMec S Boc Thr Phe Gly AMC - - - Kirisako & Ohsumi, 2004