Substrates for peptidase C50.001: separase (yeast-type)

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
cohesin subunit Scc1	Q12158	1-566	peptide-Arg180+Arg-peptide	P	Glu	Val	Gly	Arg	Arg	Phe	Ser	Pro	Rao et al., 2001	16366
cohesin subunit Scc1	Q12158	1-566	peptide-Arg268+Arg-peptide	P	Glu	Gln	Gly	Arg	Arg	Leu	Gly	Glu	Rao et al., 2001	16367
double-strand-break repair protein rad21 homolog	O60216	1-631	peptide-Arg172+Glu-peptide	P	Glu	Ile	Met	Arg	Glu	Gly	Ser	Ala	Hauf et al., 2001	16372
double-strand-break repair protein rad21 homolog	O60216	1-631	peptide-Arg450+Leu-peptide	P	Glu	Pro	Ser	Arg	Leu	Gln	Glu	Ser	Hauf et al., 2001	16373
rad21 protein	P30776	1-628	peptide-Arg179+Asn-peptide	P	Glu	Ala	Gly	Arg	Asn	Ala	Gln	Val	Peters & Nasmyth, 2004	16368
rad21 protein	P30776	1-628	peptide-Arg231+Asp-peptide	P	Glu	Val	Gly	Arg	Asp	Ala	Pro	Ala	Peters & Nasmyth, 2004	16369
rec8 protein	Q12188	1-680	peptide-Arg431+Lys-peptide	P	Glu	Arg	Gly	Arg	Lys	Arg	Ala	His	Sullivan et al., 2004	16370
rec8 protein	Q12188	1-680	peptide-Arg453+Lys-peptide	P	Glu	Tyr	Gly	Arg	Lys	Ser	Phe	Arg	Sullivan et al., 2004	16371
separase	Q14674	1-2120	peptide-Arg1486+Thr-peptide	P	Glu	Ile	Met	Arg	Thr	Ile	Pro	Glu	Peters & Nasmyth, 2004	16374
separase	Q14674	1-2120	peptide-Arg1506+Gly-peptide	P	Glu	Ile	Leu	Arg	Gly	Ser	Asp	Gly	Peters & Nasmyth, 2004	16375
separase	Q14674	1-2120	peptide-Arg1535+Leu-peptide	P	Glu	Leu	Leu	Arg	Leu	Asp	Ser	Ser	Peters & Nasmyth, 2004	16376
Slk19 protein	Q08581	1-821	peptide-Arg77+Ser-peptide	P	Asp	Tyr	Gly	Arg	Ser	Ser	Ala	Leu	Sullivan et al., 2004	16377