Substrates for peptidase C33.001: equine arteritis virus Nsp2-type cysteine peptidase

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
Equine arterivirus ORF1ab polyprotein	P19811	1-3175	peptide-Gly831+Gly-peptide	P	CS	Arg	Leu	Ile	Gly	Gly	Trp	Ile	Tyr	Ziebuhr et al., 2000
Lactate dehydrogenase elevating virus ORF1ab polyprotein	Q83017	1-3616	peptide-Gly1286+Gly-peptide	P	CS	Phe	Val	Ser	Gly	Gly	His	Val	Ala	Ziebuhr et al., 2000
polyubiquitin	P0CG47	1-76	peptide-Gly+Lys48-peptide	N		Leu	Arg	Gly	Gly	Lys	Gln	Leu	Glu	Sun et al., 2010
polyubiquitinated NF-kappa-B inhibitor alpha	P0CG47/P25963	1-76	peptide-Gly+Lys-peptide	P		Leu	Arg	Gly	Gly	Lys	Xaa	Xaa	Xaa	Sun et al., 2010
Replicase polyprotein 1ab	P19811	1-3175	peptide-Gly831+Gly-peptide	P		Arg	Leu	Ile	Gly	Gly	Trp	Ile	Tyr	Gorbalenya et al., 2004	16333