Substrates for peptidase C16.012: coronavirus COVID-19 papain-like peptidase

Summary Alignment Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
CD177 antigen	Q8N6Q3	22-408	peptide-Gly361+Gly-peptide	P	MS	Leu	Ser	Gly	Gly	Gly	Leu	Ser	Thr
interferon regulatory factor 3	Q14653	1-427	peptide-Gly270+Gly-peptide	P	NT	Cys	Leu	Gly	Gly	Gly	Leu	Ala	Leu	Moustaqil et al., 2020
interferon regulatory factor 3	Q14653	1-427	peptide-Leu272+Ala-peptide	P	NT	Gly	Gly	Gly	Leu	Ala	Leu	Trp	Arg
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gly180+Ala-peptide	P	CS	Leu	Asn	Gly	Gly	Ala	Tyr	Thr	Arg
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gly818+Ala-peptide	P	CS	Leu	Lys	Gly	Gly	Ala	Pro	Thr	Lys
replicase polyprotein 1a	P0DTC1	1-4405	peptide-Gly2763+Lys-peptide	P	CS	Leu	Lys	Gly	Gly	Lys	Ile	Val	Asn
serine/threonine-protein kinase ULK1	O75385	1-1050	peptide-Gly499+Arg-peptide	P	NT	Leu	Gly	Gly	Gly	Arg	Pro	Tyr	Thr	Mohamud et al., 2021
ubiquitin-NHMec			peptide-Gly76+NHMec	S		Leu	Arg	Gly	Gly	AMC	-	-	-	Li et al., 2020