Substrates for peptidase C02.004: calpain-3

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference	CutDB
C-terminal-binding protein 1	Q1336	1-440	Peptide-His409+Gly-peptide	P	MS	Ser	Leu	Ser	His	Gly	Leu	Pro	Pro	Ono et al., 2013
calpain-3	P16259	1-821	peptide-Asp591+Arg-peptide	P		Ile	Ser	Val	Asp	Arg	Pro	Val	Lys	Fukiage et al., 2002	16117
calpain-3	P16259	1-821	peptide-Tyr274+Gly-peptide	P		Asn	Met	Thr	Tyr	Gly	Thr	Ser	Pro	Kinbara et al., 1998	20697
calpain-3	P16259	1-821	peptide-Asn296+Ser-peptide	P		Asn	Met	Asp	Asn	Ser	Leu	Leu	Arg	Kinbara et al., 1998	20698
calpain-3	P16259	1-821	peptide-Tyr322+Glu-peptide	P		Pro	Val	Gln	Tyr	Glu	Thr	Arg	Met	Kinbara et al., 1998	20699
Calpain-3	P20807-1	1-821	peptide-Glu412+Ile-peptide	P	NT	Thr	Lys	Leu	Glu	Ile	Cys	Asn	Leu	Federici et al., 1999
Calpain-3	P20807-1	1-821	peptide-Lys30+Ala-peptide	P	NT	Ala	Asp	Ser	Lys	Ala	Thr	Glu	Ala	Federici et al., 1999
Calpastatin	P20810-2	1-708	Peptide-Gly195+Lys-Peptide	P	NT	Glu	Glu	Leu	Gly	Lys	Arg	Glu	Val	Ono et al., 2004
Calpastatin	P20810-2	1-708	Peptide-Leu209+Ala-Peptide	P	NT	Arg	Glu	Leu	Leu	Ala	Lys	Pro	Ile	Ono et al., 2004
Calpastatin	P20810-2	1-708	Peptide-Ala210+Lys-Peptide	P	NT	Glu	Leu	Leu	Ala	Lys	Pro	Ile	Gly	Ono et al., 2004
Calpastatin	P20810-2	1-708	Peptide-Cys238+Gly-Peptide	P	NT	Asp	Phe	Thr	Cys	Gly	Ser	Pro	Thr	Ono et al., 2004
Calpastatin	P20810-2	1-708	Peptide-Gly253+Thr-Peptide	P	NT	Gln	Ser	Ala	Gly	Thr	Val	Arg	Ser	Ono et al., 2004
Calpastatin	P20810-2	1-708	Peptide-Arg256+Ser-Peptide	P	NT	Gly	Thr	Val	Arg	Ser	Ala	Ala	Pro	Ono et al., 2004
Calpastatin Peptide	P20810-2	184-210	Peptide-Glu203+Lys-Peptide	S	NT	Thr	Ile	Pro	Pro	Lys	Tyr	Arg	Glu	Ono et al., 2004
PDZ and LIM domain protein 1	O00151	2-329	peptide-Phe271+Val-peptide	P	NT	Val	Gly	Val	Phe	Val	Lys	Leu	Arg	Bertipaglia et al., 2009
Titin (I80 –I83, PEVK fragment)	Q8WZ42-4	8432-9563	Peptide-Lys8563+Thr-Peptide	P	NT	Met	Leu	Lys	Lys	Thr	Pro	Ile	Leu	Sorimachi & Ono, 2012
Titin (I80 –I83, PEVK fragment)	Q8WZ42-4	8432-9563	Peptide-Ser8651+Gln-Peptide	P	NT	Gln	Arg	Leu	Ser	Gln	Thr	Glu	Pro	Sorimachi & Ono, 2012
Titin (I80 –I83, PEVK fragment)	Q8WZ42-4	8432-9563	Peptide-Gln8652+Thr-Peptide	P	NT	Arg	Leu	Ser	Gln	Thr	Glu	Pro	Val	Sorimachi & Ono, 2012
Titin (I80 –I83, PEVK fragment)	Q8WZ42-4	8432-9563	Peptide-Lys8506+Gly-Peptide	P	NT	Ile	His	Gln	Lys	Gly	Asp	Glu	Ala	Sorimachi & Ono, 2012