Substrates for peptidase A02.011: human endogenous retrovirus K retropepsin

Summary Alignment Sequences Sequence features Distribution Literature Substrates

Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.

Substrate	Uniprot	Residue range	Cleavage Site	Cleavage type	Evidence	P4	P3	P2	P1	P1'	P2'	P3'	P4'	Reference
2-aminobenzoyl-Ala-Thr-His-Gln-Val-Tyr-NPh-Val-Arg-Lys-Ala			2-aminobenzoyl-Ala-Thr-His-Gln-Val-Tyr+NPh-Val-Arg-Lys-Ala	S		His	Gln	Val	Tyr	NPh	Val	Arg	Lys
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase	P37769	12-57	peptide-Gly39+Ile-peptide	N	MS	Asp	Ile	Val	Gly	Ile	Asn	Ile	Val	Biniossek et al., 2016
2-dehydro-3-deoxygluconokinase	P37647	178-216	peptide-Phe195+Leu-peptide	N	MS	Asp	Ile	Ala	Phe	Leu	Thr	Leu	Asp	Biniossek et al., 2016
2-dehydro-3-deoxyphosphooctonate aldolase	P0A715	5-31	peptide-Leu21+Phe-peptide	N	MS	Pro	Phe	Val	Leu	Phe	Gly	Gly	Met	Biniossek et al., 2016
2-iminobutanoate/2-iminopropanoate deaminase	P0AF93	80-105	peptide-Phe94+Phe-peptide	N	MS	Tyr	Glu	Ala	Phe	Phe	Thr	Glu	His	Biniossek et al., 2016
3-mercaptopyruvate sulfurtransferase	P31142	1-27	peptide-Gly8+Ala-peptide	N	MS	Trp	Phe	Val	Gly	Ala	Asp	Trp	Leu	Biniossek et al., 2016
3-methyl-2-oxobutanoate hydroxymethyltransferase	P31057	113-129	peptide-Trp118+Leu-peptide	N	MS	Gly	Gly	Glu	Trp	Leu	Val	Glu	Thr	Biniossek et al., 2016
30S ribosomal protein S1	P0AG67	201-226	peptide-Phe208+Val-peptide	N	MS	Tyr	Gly	Ala	Phe	Val	Asp	Leu	Gly	Biniossek et al., 2016
30S ribosomal protein S13	P0A7S9	14-27	peptide-Ala18+Leu-peptide	N	MS	Ala	Val	Ile	Ala	Leu	Thr	Ser	Ile	Biniossek et al., 2016
30S ribosomal protein S19	P0A7U3	38-55	peptide-Gly46+Leu-peptide	N	MS	Asn	Met	Ile	Gly	Leu	Thr	Ile	Ala	Biniossek et al., 2016
30S ribosomal protein S6	P02358	57-79	peptide-Leu61+Met-peptide	N	MS	His	Tyr	Val	Leu	Met	Asn	Val	Glu	Biniossek et al., 2016
30S ribosomal protein S6	P02358	3-24	peptide-Phe8+Met-peptide	N	MS	Glu	Ile	Val	Phe	Met	Val	His	Pro	Biniossek et al., 2016
30S ribosomal protein S7	P02359	57-70	peptide-Ala61+Phe-peptide	N	MS	Glu	Leu	Glu	Ala	Phe	Glu	Val	Ala	Biniossek et al., 2016
30S ribosomal protein S7	P02359	57-70	peptide-Phe62+Glu-peptide	N	MS	Leu	Glu	Ala	Phe	Glu	Val	Ala	Leu	Biniossek et al., 2016
50S ribosomal protein L13	P0AA10	42-61	peptide-Tyr53+Ile-peptide	N	MS	Thr	Gly	Asp	Tyr	Ile	Ile	Val	Leu	Biniossek et al., 2016
50S ribosomal protein L16	P0ADY7	87-100	peptide-Tyr91+Trp-peptide	N	MS	Asn	Val	Glu	Tyr	Trp	Val	Ala	Leu	Biniossek et al., 2016
6-phosphogluconate dehydrogenase, decarboxylating	P00350	114-136	peptide-Gly124+Thr-peptide	N	MS	Asn	Phe	Ile	Gly	Thr	Gly	Val	Ser	Biniossek et al., 2016
6-phosphogluconate dehydrogenase, decarboxylating	P00350	243-260	peptide-Asp251+Val-peptide	N	MS	Tyr	Leu	Val	Asp	Val	Ile	Leu	Asp	Biniossek et al., 2016
60 kDa chaperonin	P0A6F5	246-268	peptide-Gly256+Glu-peptide	N	MS	Asp	Val	Glu	Gly	Glu	Ala	Leu	Ala	Biniossek et al., 2016
Acetate kinase	P0A6A3	18-43	peptide-Gly32+Leu-peptide	N	MS	Tyr	Leu	Ser	Gly	Leu	Ala	Glu	Cys	Biniossek et al., 2016
Acid stress chaperone HdeA	P0AES9	33-63	peptide-Phe42+Leu-peptide	N	MS	Cys	Glu	Asp	Phe	Leu	Ala	Val	Asp	Biniossek et al., 2016
Aconitate hydratase B	P36683	797-819	peptide-Phe804+Leu-peptide	N	MS	Ala	Asn	Val	Phe	Leu	Ala	Ser	Ala	Biniossek et al., 2016
Adenine phosphoribosyltransferase	P69503	145-166	peptide-Phe154+Ile-peptide	N	MS	Asp	Ala	Ala	Phe	Ile	Ile	Asn	Leu	Biniossek et al., 2016
Adenylosuccinate lyase	P0AB89	274-285	peptide-Tyr278+Ile-peptide	N	MS	Val	Trp	Gly	Tyr	Ile	Ala	Leu	Asn	Biniossek et al., 2016
Alanine--tRNA ligase	P00957	290-306	peptide-Phe293+Leu-peptide	N	MS	Ser	Cys	Ala	Phe	Leu	Ile	Ala	Asp	Biniossek et al., 2016
Aldehyde-alcohol dehydrogenase	P0A9Q7	318-336	peptide-Gly321+Glu-peptide	N	MS	Ile	Leu	Ile	Gly	Glu	Val	Thr	Val	Biniossek et al., 2016
Alkyl hydroperoxide reductase C	P0AE08	18-27	peptide-Phe21+Ile-peptide	N	MS	Asn	Gly	Glu	Phe	Ile	Glu	Ile	Thr	Biniossek et al., 2016
Aspartate--tRNA ligase	P21889	301-315	peptide-Phe304+Ala-peptide	N	MS	Ser	Val	Glu	Phe	Ala	Val	Phe	Ala	Biniossek et al., 2016
Bifunctional purine biosynthesis protein PurH	P15639	84-114	peptide-Met100+Val-peptide	N	MS	Pro	Ile	Asp	Met	Val	Val	Val	Asn	Biniossek et al., 2016
Chaperone protein DnaK	P0A6Y8	35-55	peptide-Ala41+Tyr-peptide	N	MS	Ser	Ile	Ile	Ala	Tyr	Thr	Gln	Asp	Biniossek et al., 2016
Chaperone protein HtpG	P0A6Z3	589-605	peptide-Trp592+Val-peptide	N	MS	Phe	Ser	Glu	Trp	Val	Glu	Leu	Leu	Biniossek et al., 2016
Coenzyme A biosynthesis bifunctional protein CoaBC	P0ABQ0	322-339	peptide-Gly326+Phe-peptide	N	MS	Tyr	Val	Val	Gly	Phe	Ala	Ala	Glu	Biniossek et al., 2016
Cytosol aminopeptidase	P68767	96-118	peptide-Ala108+Val-peptide	N	MS	Ser	Met	Glu	Ala	Val	Cys	Phe	Leu	Biniossek et al., 2016
D-serine dehydratase	P00926	24-55	peptide-Gly43+Leu-peptide	N	MS	Pro	Tyr	Val	Gly	Leu	Thr	Glu	Gln	Biniossek et al., 2016
Delta-aminolevulinic acid dehydratase	P0ACB2	304-320	peptide-Tyr312+Phe-peptide	N	MS	Ile	Phe	Ser	Tyr	Phe	Ala	Leu	Asp	Biniossek et al., 2016
Dihydrolipoyl dehydrogenase	P0A9P0	346-365	peptide-Ala358+Trp-peptide	N	MS	Pro	Glu	Val	Ala	Trp	Val	Gly	Leu	Biniossek et al., 2016
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex	P06959	271-291	peptide-Phe278+Glu-peptide	N	MS	Ile	Met	Ile	Phe	Glu	Val	Glu	Gly	Biniossek et al., 2016
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex	P06959	493-512	peptide-Gly497+Val-peptide	N	MS	Ile	Asn	Ile	Gly	Val	Ala	Val	Asp	Biniossek et al., 2016
Elongation factor 4	P60785	577-596	peptide-Phe588+Leu-peptide	N	MS	Gln	Glu	Ala	Phe	Leu	Ala	Ile	Leu	Biniossek et al., 2016
Elongation factor Tu 1	P0CE47	189-205	peptide-Leu192+Ala-peptide	N	MS	Ile	Leu	Glu	Leu	Ala	Gly	Phe	Leu	Biniossek et al., 2016
Elongation factor Tu 1	P0CE47	91-117	peptide-Gly101+Ala-peptide	N	MS	Gln	Met	Asp	Gly	Ala	Ile	Leu	Val	Biniossek et al., 2016
FKBP-type peptidyl-prolyl cis-trans isomerase FkpA	P45523	228-249	peptide-Phe241+Asp-peptide	N	MS	Thr	Leu	Val	Phe	Asp	Val	Glu	Leu	Biniossek et al., 2016
Flavodoxin 1	P61949	1-20	peptide-Phe7+Phe-peptide	N	MS	Thr	Gly	Ile	Phe	Phe	Gly	Ser	Asp	Biniossek et al., 2016
Flavodoxin 1	P61949	132-148	peptide-Gly141+Leu-peptide	N	MS	His	Phe	Val	Gly	Leu	Ala	Ile	Asp	Biniossek et al., 2016
Formate acetyltransferase 1	P09373	357-379	peptide-Leu375+Trp-peptide	N	MS	Met	Thr	Ile	Leu	Trp	Ser	Glu	Lys	Biniossek et al., 2016
Glucose-1-phosphate adenylyltransferase	P0A6V1	133-146	peptide-Tyr135+Val-peptide	N	MS	Lys	Ala	Glu	Tyr	Val	Val	Ile	Leu	Biniossek et al., 2016
Glucose-6-phosphate isomerase	P0A6T1	420-435	peptide-Phe424+Phe-peptide	N	MS	Leu	Ser	Asn	Phe	Phe	Ala	Gln	Thr	Biniossek et al., 2016
Glucose-6-phosphate isomerase	P0A6T1	476-492	peptide-Leu485+Ile-peptide	N	MS	Leu	Gly	Ala	Leu	Ile	Ala	Leu	Tyr	Biniossek et al., 2016
Glutamate decarboxylase beta	P69910	360-370	peptide-Phe365+Ile-peptide	N	MS	Pro	Tyr	Glu	Phe	Ile	Cys	Thr	Gly	Biniossek et al., 2016
Glutamate decarboxylase beta	P69910	428-442	peptide-Phe433+Ala-peptide	N	MS	Glu	Met	Asp	Phe	Ala	Glu	Leu	Leu	Biniossek et al., 2016
Glycerol dehydrogenase	P0A9S5	324-361	peptide-Tyr349+Ala-peptide	N	MS	Asp	Gln	Val	Tyr	Ala	Ala	Leu	Leu	Biniossek et al., 2016
Glycerol kinase	P0A6F3	337-362	peptide-Tyr344+Val-peptide	N	MS	Asn	Gly	Val	Tyr	Val	Val	Pro	Ala	Biniossek et al., 2016
Glycerol kinase	P0A6F3	438-464	peptide-Gly449+Leu-peptide	N	MS	Tyr	Leu	Ala	Gly	Leu	Ala	Val	Gly	Biniossek et al., 2016
Glycerophosphodiester phosphodiesterase, periplasmic	P09394	226-247	peptide-Ala237+Tyr-peptide	N	MS	Gln	Leu	Ile	Ala	Tyr	Thr	Asp	Trp	Biniossek et al., 2016
Glycine dehydrogenase (decarboxylating)	P33195	206-237	peptide-Tyr229+Thr-peptide	N	MS	Ile	His	Asp	Tyr	Thr	Ala	Leu	Ile	Biniossek et al., 2016
GMP synthase [glutamine-hydrolyzing]	P04079	147-173	peptide-Phe154+Ile-peptide	N	MS	Pro	Ser	Asp	Phe	Ile	Thr	Val	Ala	Biniossek et al., 2016
HERV-K(C19) Gag protein	Q9YNA8	2-666	Peptide-Tyr134+Val-Peptide	P	NT	His	Cys	Glu	Tyr	Val	Ala	Glu	Pro	Kraus et al., 2011
HERV-K(C19) Gag protein	Q9YNA8	2-666	Peptide-Asp148+Tyr-Peptide	P	NT	Gln	Asn	Val	Asp	Tyr	Asn	Gln	Leu	Kraus et al., 2011
HERV-K(C19) Gag protein	Q9YNA8	2-666	Peptide-Phe252+Pro-Peptide	P	NT	Ala	Trp	Gln	Phe	Pro	Val	Thr	Leu	Kraus et al., 2011
HERV-K(C19) Gag protein	Q9YNA8	2-666	Peptide-Gly532+Val-Peptide	P	NT	Ala	Ile	Thr	Gly	Val	Val	Leu	Gly	Kraus et al., 2011
Histidine--tRNA ligase	P60906	312-324	peptide-Leu314+Leu-peptide	N	MS	Arg	Leu	Val	Leu	Leu	Val	Gln	Ala	Biniossek et al., 2016
human endogenous retrovirus K polyprotein	P87892	1-334	peptide-Tyr175+Trp-peptide	P		Lys	Ala	Ala	Tyr	Trp	Ala	Ser	Gln
Isoleucine--tRNA ligase	P00956	832-851	peptide-Ala838+Ala-peptide	N	MS	Ser	Leu	Glu	Ala	Ala	Val	Thr	Leu	Biniossek et al., 2016
KHG/KDPG aldolase	P0A955	177-199	peptide-Gly182+Gly-peptide	N	MS	Leu	Cys	Ile	Gly	Gly	Ser	Trp	Leu	Biniossek et al., 2016
L-threonine 3-dehydrogenase	P07913	118-134	peptide-Tyr124+Leu-peptide	N	MS	Phe	Ala	Glu	Tyr	Leu	Val	Ile	Pro	Biniossek et al., 2016
L-threonine dehydratase catabolic TdcB	P0AGF6	253-267	peptide-Asp257+Ile-peptide	N	MS	Leu	Val	Asp	Asp	Ile	Val	Leu	Val	Biniossek et al., 2016
Lys-Ala-Arg-Val-Tyr-NPh-Glu-Ala-Nle-NH2			Lys-Ala-Arg-Val-Tyr+Nph-Glu-Ala-Nle-NH2	S		Ala	Arg	Val	Tyr	NPh	Glu	Ala	Nle
Maltose/maltodextrin-binding periplasmic protein	P0AEX9	283-299	peptide-Gly286+Val-peptide	N	MS	Pro	Phe	Val	Gly	Val	Leu	Ser	Ala	Biniossek et al., 2016
Methionine--tRNA ligase	P00959	219-234	peptide-Trp222+Phe-peptide	N	MS	Met	Gln	Glu	Trp	Phe	Glu	Ser	Gly	Biniossek et al., 2016
NAD-dependent dihydropyrimidine dehydrogenase subunit PreA	P25889	292-331	peptide-Met315+Val-peptide	N	MS	Leu	Gln	Glu	Met	Val	Gly	Leu	Ala	Biniossek et al., 2016
Outer membrane porin C	P06996	248-267	peptide-Tyr254+Leu-peptide	N	MS	Asn	Asn	Ile	Tyr	Leu	Ala	Ala	Gln	Biniossek et al., 2016
Outer membrane protein A	P0A910	135-159	peptide-Gly146+Gly-peptide	N	MS	Val	Phe	Ala	Gly	Gly	Val	Glu	Tyr	Biniossek et al., 2016
Peptidyl-prolyl cis-trans isomerase B	P23869	44-60	peptide-Phe48+Met-peptide	N	MS	Ile	Asn	Gly	Phe	Met	Ile	Gln	Gly	Biniossek et al., 2016
PFL-like enzyme TdcE	P42632	133-146	peptide-Tyr140+Leu-peptide	N	MS	Glu	Phe	Glu	Tyr	Leu	Phe	Thr	Asp	Biniossek et al., 2016
PFL-like enzyme TdcE	P42632	361-389	peptide-Leu379+Trp-peptide	N	MS	Leu	Thr	Ile	Leu	Trp	Ser	Glu	Glu	Biniossek et al., 2016
Phosphate acetyltransferase	P0A9M8	361-383	peptide-Asp372+Trp-peptide	N	MS	Ile	Asn	Ala	Asp	Trp	Ile	Glu	Ser	Biniossek et al., 2016
Phosphoenolpyruvate carboxylase	P00864	774-785	peptide-Trp777+Leu-peptide	N	MS	Ala	Asp	Leu	Trp	Leu	Ala	Glu	Tyr	Biniossek et al., 2016
Phosphoribosylformylglycinamidine cyclo-ligase	P08178	175-198	peptide-Ala183+Leu-peptide	N	MS	Val	Leu	Ile	Ala	Leu	Gly	Ser	Ser	Biniossek et al., 2016
Pyruvate dehydrogenase E1 component	P0AFG8	251-264	peptide-Asn253+Leu-peptide	N	MS	Lys	Leu	Asp	Asn	Leu	Val	Phe	Val	Biniossek et al., 2016
Ribose import binding protein RbsB	P02925	27-45	peptide-Ala30+Leu-peptide	N	MS	Asp	Thr	Ile	Ala	Leu	Val	Val	Ser	Biniossek et al., 2016
Ribose import binding protein RbsB	P02925	27-45	peptide-Leu31+Val-peptide	N	MS	Thr	Ile	Ala	Leu	Val	Val	Ser	Thr	Biniossek et al., 2016
Ribosome-recycling factor	P0A805	32-52	peptide-Gly39+Ile-peptide	N	MS	Leu	Leu	Asp	Gly	Ile	Val	Val	Glu	Biniossek et al., 2016
S-adenosylmethionine synthase	P0A817	178-197	peptide-Ala183+Val-peptide	N	MS	Gly	Ile	Asp	Ala	Val	Val	Leu	Ser	Biniossek et al., 2016
Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln			Ser-Gln-Asn-Tyr+Pro-Ile-Val-Gln	N		Ser	Gln	Asn	Tyr	Pro	Ile	Val	Gln
Serine hydroxymethyltransferase	P0A825	26-42	peptide-Leu32+Ile-peptide	N	MS	His	Ile	Glu	Leu	Ile	Ala	Ser	Glu	Biniossek et al., 2016
Serine hydroxymethyltransferase	P0A825	64-81	peptide-Tyr70+Val-peptide	N	MS	Gly	Cys	Glu	Tyr	Val	Asp	Ile	Val	Biniossek et al., 2016
Stringent starvation protein A	P0ACA3	109-129	peptide-Leu113+Met-peptide	N	MS	Trp	Tyr	Thr	Leu	Met	Asn	Thr	Ile	Biniossek et al., 2016
Transcription termination/antitermination protein NusG	P0AFG0	136-155	peptide-Gly146+Val-peptide	N	MS	Asp	Phe	Asn	Gly	Val	Val	Glu	Glu	Biniossek et al., 2016
Translation initiation factor IF-2	P0A705	458-487	peptide-Leu467+Val-peptide	N	MS	Ile	Val	Val	Leu	Val	Val	Ala	Ala	Biniossek et al., 2016
Translation initiation factor IF-2	P0A705	309-324	peptide-Gly313+Glu-peptide	N	MS	Val	Val	Ile	Gly	Glu	Thr	Ile	Thr	Biniossek et al., 2016
Triosephosphate isomerase	P0A858	153-176	peptide-Gly161+Ala-peptide	N	MS	Ala	Phe	Glu	Gly	Ala	Val	Ile	Ala	Biniossek et al., 2016
Tryptophanase	P0A853	34-69	peptide-Phe47+Ile-peptide	N	MS	Glu	Asp	Val	Phe	Ile	Asp	Leu	Leu	Biniossek et al., 2016
Tryptophanase	P0A853	128-151	peptide-Tyr134+Phe-peptide	N	MS	Phe	Ser	Asn	Tyr	Phe	Phe	Asp	Thr	Biniossek et al., 2016
Tryptophanase	P0A853	335-366	peptide-Gly349+Val-peptide	N	MS	Glu	Glu	Ile	Gly	Val	Val	Cys	Gln	Biniossek et al., 2016
Tryptophanase	P0A853	179-211	peptide-Ala193+Thr-peptide	N	MS	Tyr	Ile	Val	Ala	Thr	Ile	Thr	Ser	Biniossek et al., 2016
Uncharacterized lipoprotein YjbH	P32689	249-261	peptide-Asp251+Trp-peptide	N	MS	Arg	Val	Thr	Asp	Trp	Ala	Asp	Val	Biniossek et al., 2016
Uncharacterized protein YecA	P0AD05	139-155	peptide-Ala143+Ile-peptide	N	MS	Ala	Leu	Glu	Ala	Ile	Ala	Leu	His	Biniossek et al., 2016
Uncharacterized protein YfeS	P78271	154-166	peptide-Met157+Asp-peptide	N	MS	Tyr	Ile	Ala	Met	Asp	Ser	Ile	Leu	Biniossek et al., 2016
Uncharacterized sulfatase YdeN	P77318	487-505	peptide-Tyr494+Thr-peptide	N	MS	Ser	Leu	Val	Tyr	Thr	Val	Glu	Asn	Biniossek et al., 2016
Uracil phosphoribosyltransferase	P0A8F0	156-171	peptide-Leu159+Val-peptide	N	MS	Val	Leu	Val	Leu	Val	Ala	Ala	Pro	Biniossek et al., 2016