Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 1 |
unknown peptidase |
1-289 |
P |
release of an initiating methionine |
MS |
Van Damme P et al., 2005 |
| 9 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 18 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 24 |
peptidyl-Lys metallopeptidase |
1-289 |
N |
|
MS |
|
| 31 |
matrix metallopeptidase-2 |
24-42 |
N |
|
MS |
Schilling & Overall, 2008 |
| 33 |
meprin alpha subunit |
1-289 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 33 |
meprin beta subunit |
1-289 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 39 |
meprin beta subunit |
1-289 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 40 |
peptidyl-Lys metallopeptidase |
1-289 |
N |
|
MS |
|
| 42 |
glutamyl endopeptidase I |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 57 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 59 |
glutamyl endopeptidase I |
57-70 |
N |
|
MS |
Schilling & Overall, 2008 |
| 61 |
cathepsin K |
57-70 |
N |
|
MS |
Schilling & Overall, 2008 |
| 70 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 88 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 93 |
cathepsin B |
89-109 |
N |
|
MS |
Biniossek et al., 2011 |
| 94 |
astacin |
91-109 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 95 |
cathepsin L |
89-109 |
N |
|
MS |
Biniossek et al., 2011 |
| 95 |
cathepsin S |
89-109 |
N |
|
MS |
Biniossek et al., 2011 |
| 98 |
matrix metallopeptidase-2 |
88-109 |
N |
|
MS |
Schilling & Overall, 2008 |
| 109 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 117 |
meprin alpha subunit |
1-289 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 117 |
meprin beta subunit |
1-289 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 139 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 142 |
matrix metallopeptidase-2 |
139-155 |
N |
|
MS |
Schilling & Overall, 2008 |
| 153 |
caspase-6 |
1-289 |
P |
|
|
Klaiman et al., 2008 |
| 155 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 162 |
meprin alpha subunit |
1-289 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 162 |
meprin beta subunit |
1-289 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 165 |
caspase-6 |
1-289 |
P |
|
|
Klaiman et al., 2008 |
| 176 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 177 |
trypsin 1 |
1-289 |
N |
|
MS |
Schilling & Overall, 2008 |
| 178 |
peptidyl-Lys metallopeptidase |
1-289 |
N |
|
MS |
|
| 182 |
matrix metallopeptidase-2 |
178-191 |
N |
|
MS |
Schilling & Overall, 2008 |
| 191 |
trypsin 1 |
1-132 |
N |
|
MS |
Schilling & Overall, 2008 |
| 193 |
peptidyl-Lys metallopeptidase |
1-289 |
N |
|
MS |
|
| 194 |
trypsin 1 |
1-132 |
N |
|
MS |
Schilling & Overall, 2008 |
| 202 |
matrix metallopeptidase-2 |
195-211 |
N |
|
MS |
Schilling & Overall, 2008 |
| 211 |
trypsin 1 |
1-132 |
N |
|
MS |
Schilling & Overall, 2008 |
| 213 |
trypsin 1 |
1-132 |
N |
|
MS |
Schilling & Overall, 2008 |
| 221 |
trypsin 1 |
1-132 |
N |
|
MS |
Schilling & Overall, 2008 |
| 237 |
peptidyl-Lys metallopeptidase |
1-289 |
N |
|
MS |
|
| 238 |
trypsin 1 |
1-132 |
N |
|
MS |
Schilling & Overall, 2008 |
| 242 |
cathepsin B |
239-253 |
N |
|
MS |
Biniossek et al., 2011 |
| 252 |
peptidyl-Lys metallopeptidase |
1-289 |
N |
|
MS |
|
| 253 |
trypsin 1 |
1-132 |
N |
|
MS |
Schilling & Overall, 2008 |
| 279 |
caspase-6 |
1-289 |
P |
|
|
Klaiman et al., 2008 |
| 281 |
caspase-6 |
1-289 |
P |
|
|
Klaiman et al., 2008 |
