Peptide and protein substrates that are thought to be physiologically relevant are indicated by P.
Peptide and protein substrates that are not physiologically relevant are indicated by N.
How cleavage sites have been identified are indicated by the following evidence codes:
NT = N-terminal sequencing,
MS = mass spectroscopy,
MU = mutation,
CS = consensus sequence,
LC = liquid chromatography.
To see all annotated cleavages for a peptidase, click on the peptidase name.
| Cleavage Site |
Peptidase |
Residue range |
Cleavage type |
Description |
Evidence |
Reference |
|---|
| 24 |
unknown peptidase |
1-505 |
P |
release of a signal peptide |
NT |
Hochstrasser et al., 1992 |
| 60 |
peptidyl-Lys metallopeptidase |
1-505 |
N |
|
MS |
|
| 74 |
peptidyl-Lys metallopeptidase |
1-505 |
N |
|
MS |
|
| 119 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 127 |
matrix metallopeptidase-2 |
122-142 |
N |
|
MS |
Schilling & Overall, 2008 |
| 129 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 130 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 138 |
matrix metallopeptidase-2 |
130-147 |
N |
|
MS |
Schilling & Overall, 2008 |
| 140 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 142 |
glutamyl endopeptidase I |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 143 |
glutamyl endopeptidase I |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 144 |
glutamyl endopeptidase I |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 145 |
peptidyl-Lys metallopeptidase |
1-505 |
N |
|
MS |
|
| 147 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 152 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 162 |
caspase-1 |
25-505 |
N |
|
MS |
Agard et al., 2010 |
| 165 |
cathepsin L |
153-173 |
N |
|
MS |
Biniossek et al., 2011 |
| 165 |
cathepsin S |
153-173 |
N |
|
MS |
Biniossek et al., 2011 |
| 165 |
cathepsin K |
152-173 |
N |
|
MS |
Schilling & Overall, 2008 |
| 170 |
glutamyl endopeptidase I |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 173 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 224 |
glutamyl endopeptidase I |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 233 |
peptidyl-Lys metallopeptidase |
1-505 |
N |
|
MS |
|
| 235 |
chymotrypsin A (cattle-type) |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 238 |
glutamyl endopeptidase I |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 251 |
peptidyl-Lys metallopeptidase |
1-505 |
N |
|
MS |
|
| 305 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 317 |
cathepsin S |
306-329 |
N |
|
MS |
Biniossek et al., 2011 |
| 329 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 336 |
chymotrypsin A (cattle-type) |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 342 |
chymotrypsin A (cattle-type) |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 350 |
glutamyl endopeptidase I |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 351 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 352 |
chymotrypsin A (cattle-type) |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 354 |
meprin beta subunit |
351-363 |
N |
|
MS |
Becker-Pauly et al., 2011 |
| 362 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 363 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 364 |
chymotrypsin A (cattle-type) |
25-505 |
N |
|
|
Silvennoinen et al., 2004 |
| 366 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 379 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 383 |
cathepsin S |
380-395 |
N |
|
MS |
Biniossek et al., 2011 |
| 433 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 448 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 460 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 482 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
| 496 |
trypsin 1 |
25-505 |
N |
|
MS |
Schilling & Overall, 2008 |
