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Literature for peptidase S08.070: kexin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, K Knockout, P Specificity, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2024
  1. Wu,L., Bian,W., Abubakar,Y.S., Lin,J., Yan,H., Zhang,H., Wang,Z., Wu,C., Shim,W. and Lu,G.D.
    FvKex2 is required for development, virulence, and mycotoxin production in Fusarium verticillioides
    Appl Microbiol Biotechnol108, 228-228. PubMed  Europe PubMed DOI
    2. 2023
  2. Jin,Y., Yang,N., Teng,D., Hao,Y., Mao,R. and Wang,J.
    Molecular Modification of Kex2 P1' Site Enhances Expression and Druggability of Fungal Defensin
    Antibiotics (Basel)12, PubMed  Europe PubMed DOI
  3. Kim,M.J., Park,S.L., Kim,S.H., Park,H.J., Sung,B.H., Sohn,J.H. and Bae,J.H.
    Modulation of Kex2p Cleavage Site for In Vitro Processing of Recombinant Proteins Produced by Saccharomyces cerevisiae
    J Microbiol Biotechnol33, 1-9. PubMed  Europe PubMed DOI
    4. 2021
  4. de Souza,A.A., de Andrade,D.M., Siqueira,F.D.S., Di Iorio,J.F., Veloso,M.P., Coelho,C.M., Viegas Junior,C., Gontijo,V.S., Dos Santos,M.H., Meneghetti,M.C.Z., Nader,H.B., Tersariol,I.L.D.S., Juliano,L., Juliano,M.A. and Judice,W.A.S.
    Semysinthetic biflavonoid Morelloflavone-7,4',7'',3''',4'''-penta-O-butanoyl is a more potent inhibitor of Proprotein Convertases Subtilisin/Kexin PC1/3 than Kex2 and Furin
    Biochim Biophys Acta Gen Subj1865, 130016-130016. PubMed  Europe PubMed DOI  I
    5. 2020
  5. Tomas,A.L., Cardoso,F., de Sousa,B. and Matos,O.
    Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
    Eur J Clin Microbiol Infect Dis39, 2205-2209. PubMed  Europe PubMed DOI
    6. 2018
  6. Huang,Y., Long,Y., Li,S., Lin,T., Wu,J., Zhang,Y. and Lin,Y.
    Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris
    Microb Cell Fact17, 172-172. PubMed  Europe PubMed DOI
    7. 2017
  7. Antunes,A.A., Jesus,L.O.P., Manfredi,M.A., de Souza,A.A., Machado,M.F.M., E Silva,P.M., Icimoto,M.Y., Juliano,M.A., Juliano,L. and Judice,W.A.S.
    Thermodynamic analysis of Kex2 activity: the acylation and deacylation steps are potassium- and substrate-dependent
    Biophys Chem235, 29-39. PubMed  Europe PubMed DOI
  8. Koseki,T., Otsuka,M., Mizuno,T. and Shiono,Y.
    Mutational analysis of Kex2 recognition sites and a disulfide bond in tannase from Aspergillus oryzae
    Biochem Biophys Res Commun482, 1165-1169. PubMed  Europe PubMed DOI
    9. 2016
  9. Manfredi,M.A., Antunes,A.A., Jesus,L.O., Juliano,M.A., Juliano,L. and Judice,W.A.
    Specificity characterization of the alpha-mating factor hormone by Kex2 protease
    Biochimie131, 149-158. PubMed  Europe PubMed DOI  P
  10. Mizutani,O., Shiina,M., Yoshimi,A., Sano,M., Watanabe,T., Yamagata,Y., Nakajima,T., Gomi,K. and Abe,K.
    Substantial decrease in cell wall alpha-1,3-glucan caused by disruption of the kexB gene encoding a subtilisin-like processing protease in Aspergillus oryzae
    Biosci Biotechnol Biochem80, 1781-1791. PubMed  Europe PubMed DOI
    11. 2015
  11. Bondaryk,M., Lukowska-Chojnacka,E. and Staniszewska,M.
    Tetrazole activity against Candida albicans. The role of KEX2 mutations in the sensitivity to (+/-)-1-[5-(2-chlorophenyl)-2H-tetrazol-2-yl]propan-2-yl acetate
    Bioorg Med Chem Lett25, 2657-2663. PubMed  Europe PubMed DOI
  12. Sreenivas,S., Krishnaiah,S.M., Govindappa,N., Basavaraju,Y., Kanojia,K., Mallikarjun,N., Natarajan,J., Chatterjee,A. and Sastry,K.N.
    Enhancement in production of recombinant two-chain Insulin Glargine by over-expression of Kex2 protease in Pichia pastoris
    Appl Microbiol Biotechnol99, 327-336. PubMed  Europe PubMed DOI
  13. Wang,J., Zhou,H., Lu,H., Du,T., Luo,Y., Wilson,I.B. and Jin,C.
    Kexin-like endoprotease KexB is required for N-glycan processing, morphogenesis and virulence in Aspergillus fumigatus
    Fungal Genet Biol76, 57-69. PubMed  Europe PubMed DOI
    14. 2012
  14. Fuller,R.S.
    Kexin
    [ISSN:978-0-12-407742-3]3, 3270-3277. DOI
    15. 2010
  15. Duan,Y., Zhu,D., Chen,J., Qin,Y. and Wang,J.
    Gene cloning, expression, and immunological characterization of kexin in rat Pneumocystis carinii
    Lab Med41, 99-103. DOI
    16. 2009
  16. Jacob-Wilk,D., Turina,M., Kazmierczak,P. and Van Alfen,N.K.
    Silencing of Kex2 significantly diminishes the virulence of Cryphonectria parasitica
    Mol Plant Microbe Interact22, 211-221. PubMed  Europe PubMed DOI  K
  17. Mizutani,O., Furukawa,K., Ichiyanagi,S., Matsuda,Y., Tokuoka,M., Fujioka,T., Yamagata,Y., Gomi,K. and Abe,K.
    Alternative processing of proproteins in aspergilli KexB gene disruptants under hyperosmotic conditions
    Biosci Biotechnol Biochem73, 40-46. PubMed  Europe PubMed DOI  K
  18. Rholam,M. and Fahy,C.
    Processing of peptide and hormone precursors at the dibasic cleavage sites
    Cell Mol Life Sci66, 2075-2091. PubMed  Europe PubMed DOI
    19. 2008
  19. Bader,O., Krauke,Y. and Hube,B.
    Processing of predicted substrates of fungal Kex2 proteinases from Candida albicans, C. glabrata, Saccharomyces cerevisiae and Pichia pastoris
    BMC Microbiol8, 116-116. PubMed  Europe PubMed DOI  P
  20. Mora-Montes,H.M., Bader,O., Lopez-Romero,E., Zinker,S., Ponce-Noyola,P., Hube,B., Gow,N.A. and Flores-Carreon,A.
    Kex2 protease converts the endoplasmic reticulum alpha1,2-mannosidase of Candida albicans into a soluble cytosolic form
    Microbiology (Reading)154, 3782-3794. PubMed  Europe PubMed DOI
  21. Pozzuolo,S., Breme,U., Salis,B., Taylor,G., Tonon,G. and Orsini,G.
    Efficient bacterial expression of fusion proteins and their selective processing by a recombinant Kex-1 protease
    Protein Expr Purif59, 334-341. PubMed  Europe PubMed DOI
  22. Torres,F.A., Vilaca,R., De Moraes,L.M.P., Reis,V.C. and Felipe,M.S.
    Expression of a kexin-like gene from the human pathogenic fungus Paracoccidioides brasiliensis in Saccharomyces cerevisiae
    Med Mycol46, 385-388. PubMed  Europe PubMed DOI  E
    23. 2007
  23. [YEAR:15-1-2007]Heiman,M.G., Engel,A. and Walter,P.
    The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating
    J Cell Biol176, 209-222. PubMed  Europe PubMed DOI
  24. Lazar,N., Brakch,N., Panchal,M., Fahy,C. and Rholam,M.
    Reactivity of basic amino acid pairs in prohormone processing: model of pro-ocytocin/neurophysin processing domain
    Arch Biochem Biophys463, 231-236. PubMed  Europe PubMed DOI  P
  25. Wheatley,J.L. and Holyoak,T.
    Differential P1 arginine and lysine recognition in the prototypical proprotein convertase Kex2
    Proc Natl Acad Sci U S A104, 6626-6631. PubMed  Europe PubMed DOI  P  S
    26. 2005
  26. [YEAR:2-12-2005]Han,H.E., Rho,S.H., Lee,Y.J. and Park,W.J.
    Engineering of Kex2 variants exhibiting altered substrate specificity
    Biochem Biophys Res Commun337, 1102-1106. PubMed  Europe PubMed DOI
  27. [YEAR:14-1-2005]Henrich,S., Lindberg,I., Bode,W. and Than,M.E.
    Proprotein convertase models based on the crystal structures of furin and kexin: explanation of their specificity
    J Mol Biol345, 211-227. PubMed  Europe PubMed DOI  S
  28. [YEAR:14-2-2005]Wachter,A. and Schwappach,B.
    The yeast CLC chloride channel is proteolytically processed by the furin-like protease Kex2p in the first extracellular loop
    FEBS Lett579, 1149-1153. PubMed  Europe PubMed DOI
  29. Werten,M.W. and de Wolf,F.A.
    Reduced proteolysis of secreted gelatin and Yps1-mediated alpha-factor leader processing in a Pichia pastoris kex2 disruptant
    Appl Environ Microbiol71, 2310-2317. PubMed  Europe PubMed DOI
    30. 2004
  30. Fuller,R.S.
    Kexin
    [ISSN:0-12-079610-4]2, 1849-1854.  V
  31. [YEAR:9-3-2004]Holyoak,T., Kettner,C.A., Petsko,G.A., Fuller,R.S. and Ringe,D.
    Structural basis for differences in substrate selectivity in Kex2 and furin protein convertases
    Biochemistry43, 2412-2421. PubMed  Europe PubMed DOI  S  I
  32. Rockwell,N.C. and Thorner,J.W.
    The kindest cuts of all: crystal structures of Kex2 and furin reveal secrets of precursor processing
    Trends Biochem Sci29, 80-87. PubMed  Europe PubMed DOI  S
    33. 2003
  33. [YEAR:10-6-2003]Holyoak,T., Wilson,M.A., Fenn,T.D., Kettner,C.A., Petsko,G.A., Fuller,R.S. and Ringe,D.
    2.4 A resolution crystal structure of the prototypical hormone-processing protease Kex2 in complex with an Ala-Lys-Arg boronic acid inhibitor
    Biochemistry42, 6709-6718. PubMed  Europe PubMed DOI  S  I
  34. Lesage,G., Guimond,J. and Boileau,G.
    trans-Complementation assay establishes the role of proregion hydrophobic amino acid residues in the biosynthesis of Saccharomyces cerevisiae Kex2p endoprotease
    Yeast20, 397-406. PubMed  Europe PubMed DOI
  35. [YEAR:5-12-2003]Punt,P.J., Drint-Kuijvenhoven,A., Lokman,B.C., Spencer,J.A., Jeenes,D., Archer,D.A. and van den Hondel,C.A.
    The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins. Evidence for alternative processing of recombinant (fusion-) proteins
    J Biotechnol106, 23-32. PubMed  Europe PubMed DOI
  36. Tomishige,N., Noda,Y., Adachi,H., Shimoi,H., Takatsuki,A. and Yoda,K.
    Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae
    Mol Genet Genomics269, 562-573. PubMed  Europe PubMed DOI
    37. 2002
  37. Rockwell,N.C., Krysan,D.J., Komiyama,T. and Fuller,R.S.
    Precursor processing by kex2/furin proteases
    Chem Rev102, 4525-4548. PubMed  Europe PubMed DOI
  38. Venancio,E.J., Daher,B.S., Andrade,R.V., Soares,C.M., Pereira,I.S. and Felipe,M.S.
    The kex2 gene from the dimorphic and human pathogenic fungus Paracoccidioides brasiliensis
    Yeast19, 1221-1231. PubMed  Europe PubMed DOI
    39. 2001
  39. Bader,O., Schaller,M., Klein,S., Kukula,J., Haack,K., Muhlschlegel,F., Korting,H.C., Schafer,W. and Hube,B.
    The KEX2 gene of Candida glabrata is required for cell surface integrity
    Mol Microbiol41, 1431-1444. PubMed  Europe PubMed DOI
  40. [YEAR:31-8-2001]Kwon,B.K., Han,K.H., Han,K.Y., Ju,S.M., Hwang,S.G., Jeon,B.H., Han,D.M. and Kim,W.S.
    Molecular cloning of kpcA gene encoding a Kex2p-like endoprotease from Aspergillus nidulans
    Mol Cells12, 142-147. PubMed  Europe PubMed
  41. [YEAR:23-11-2001]Lesage,G., Tremblay,M., Guimond,J. and Boileau,G.
    Mechanism of Kex2p inhibition by its proregion
    FEBS Lett508, 332-336. PubMed  Europe PubMed DOI
  42. [YEAR:15-4-2001]Oda,K., Oyama,H., Ito,S., Fukiharu,M., Miyagawa,Y., Takahashi,S., Hirose,M., Kikuchi,N., Nakayama,T. and Shibano,Y.
    Cloning and rational mutagenesis of kexstatin I, a potent proteinaceous inhibitor of Kex2 proteinase
    Biochem J355, 339-346. PubMed  Europe PubMed DOI
  43. [YEAR:19-10-2001]Rockwell,N.C. and Fuller,R.S.
    Differential utilization of enzyme-substrate interactions for acylation but not deacylation during the catalytic cycle of Kex2 protease
    J Biol Chem276, 38394-38399. PubMed  Europe PubMed DOI
    44. 2000
  44. Jalving,R., Van de Vondervoort,P.J.I., Visser,J. and Schaap,P.J.
    Characterization of the kexin-like maturase of Aspergillus niger
    Appl Environ Microbiol66, 363-368. PubMed  Europe PubMed
  45. [YEAR:12-12-2000]Komiyama,T. and Fuller,R.S.
    Engineered eglin c variants inhibit yeast and human proprotein processing proteases, Kex2 and furin
    Biochemistry39, 15156-15165. PubMed  Europe PubMed DOI
    46. 1998
  46. [YEAR:1-9-1998]Bevan,A., Brenner,C. and Fuller,R.S.
    Quantitative assessment of enzyme specificity in vivo: P2 recognition by Kex2 protease defined in a genetic system
    Proc Natl Acad Sci U S A95, 10384-10389. PubMed  Europe PubMed DOI  P
  47. Goller,S.P., Schoisswohl,D., Baron,M., Parriche,M. and Kubicek,C.P.
    Role of endoproteolytic dibasic proprotein processing in maturation of secretory proteins in Trichoderma reesei
    Appl Environ Microbiol64, 3202-3208. PubMed  Europe PubMed
  48. Oluwatosin,Y.E. and Kane,P.M.
    Mutations in the yeast KEX2 gene cause a Vma(-)-like phenotype: a possible role for the KEX2 endoprotease in vacuolar acidification
    Mol Cell Biol18, 1534-1543. PubMed  Europe PubMed
  49. Powner,D. and Davey,J.
    Activation of the kexin from Schizosaccharomyces pombe requires internal cleavage of its initially cleaved prosequence
    Mol Cell Biol18, 400-408. PubMed  Europe PubMed
  50. [YEAR:10-3-1998]Rockwell,N.C. and Fuller,R.S.
    Interplay between S1 and S4 subsites in Kex2 protease: Kex2 exhibits dual specificity for the P4 side chain
    Biochemistry37, 3386-3391. PubMed  Europe PubMed DOI  P
    51. 1997
  51. [YEAR:14-11-1997]Newport,G. and Agabian,N.
    KEX2 influences Candida albicans proteinase secretion and hyphal formation
    J Biol Chem272, 28954-28961. PubMed  Europe PubMed DOI
  52. Rockwell,N.C., Wang,G.T., Krafft,G.A. and Fuller,R.S.
    Internally consistent libraries of fluorogenic substrates demonstrate that Kex2 protease specificity is generated by multiple mechanisms
    Biochemistry36, 1912-1917. PubMed  Europe PubMed DOI  P
    53. 1995
  53. Chaudhuri,B. and Stephan,C.
    A C-terminal domain, which prevents secretion of the neuroendocrine protein 7B2 in Saccharomyces cerevisiae, inhibits Kex2 yet is processed by the Yap3 protease
    FEBS Lett364, 91-97. PubMed  Europe PubMed DOI
  54. Diefenbach-Jagger,H., Brenner,C., Kemp,B.E., Baron,W., McLean,J., Martin,T.J. and Moseley,J.M.
    Arg21 is the preferred kexin cleavage site in parathyroid-hormone-related protein
    Eur J Biochem229, 91-98. PubMed  Europe PubMed DOI
  55. Komano,H. and Fuller,R.S.
    Shared functions in vivo of a glycosyl-phosphatidylinositol-linked aspartyl protease, Mkc7, and the proprotein processing protease Kex2 in yeast
    Proc Natl Acad Sci U S A92, 10752-10756. PubMed  Europe PubMed DOI
    56. 1994
  56. Bourbonnais,Y., Germain,D., Ash,J. and Thomas,D.Y.
    Cleavage of prosomatostatins by the yeast Yap3 and Kex2 endoprotease
    Biochimie76, 226-233. PubMed  Europe PubMed
  57. Davey,J., Davis,K., Imai,Y., Yamamoto,M. and Matthews,G.
    Isolation and characterization of krp, a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe
    EMBO J13, 5910-5921. PubMed  Europe PubMed
  58. Enderlin,C.S. and Ogrydziak,D.M.
    Cloning, nucleotide sequence and functions of XPR6, which codes for a dibasic processing endoprotease from the yeast Yarrowia lipolytica
    Yeast10, 67-79. PubMed  Europe PubMed DOI
    59. 1993
  59. Brenner,C., Bevan,A. and Fuller,R.S.
    Specificity and activity of the kex2 protease: from yeast genetics to enzyme kinetics
    Protein Eng Des Sel6, 42- DOI
    60. 1992
  60. [YEAR:16-3-1992]Germain,D., Dumas,F., Vernet,T., Bourbonnais,Y., Thomas,D.Y. and Boileau,G.
    The pro-region of the Kex2 endoprotease of Saccharomyces cerevisiae is removed by self-processing
    FEBS Lett299, 283-286. PubMed  Europe PubMed DOI
    61. 1991
  61. Fabre,E., Nicaud,J.M., Lopez,M.C. and Gaillardin,C.
    Role of the proregion in the production and secretion of the Yarrowia lipolytica alkaline extracellular protease
    J Biol Chem266, 3782-3790. PubMed  Europe PubMed
    62. 1990
  62. van den Ouweland,A.M.W., van Duijnhoven,H.L.P., Keizer,G.D., Dorssers,L.C.J. and Van de Ven,W.J.
    Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2
    Nucleic Acids Res18, 664-664. PubMed  Europe PubMed DOI
    63. 1989
  63. Fuller,R.S., Brake,A. and Thorner,J.
    Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease
    Proc Natl Acad Sci U S A86, 1434-1438. PubMed  Europe PubMed
  64. Mizuno,K., Nakamura,T., Ohshima,T., Tanaka,S. and Matsuo,H.
    Characterization of KEX2-encoded endopeptidase from yeast Saccharomyces cerevisiae
    Biochem Biophys Res Commun159, 305-311. PubMed  Europe PubMed  I
    65. 1988
  65. Mizuno,K., Nakamura,T., Ohshima,T., Tanaka,S. and Matsuo,H.
    Yeast KEX2 gene encodes an endopeptidase homologous to subtilisin-like serine proteases
    Biochem Biophys Res Commun156, 246-254. PubMed  Europe PubMed
  66. Tanguy-Rougeau,C., Wesolowski-Louvel,M. and Fukuhara,H.
    The Kluyveromyces lactis KEX1 gene encodes a subtilisin-type serine proteinase
    FEBS Lett234, 464-470. PubMed  Europe PubMed
  67. [YEAR:8-7-1988]Thomas,G., Thorne,B.A., Thomas,L., Allen,R.G., Hruby,D.E., Fuller,R. and Thorner,J.
    Yeast KEX2 endopeptidase correctly cleaves a neuroendocrine prohormone in mammalian cells
    Science241, 226-230. PubMed  Europe PubMed
    68. 1987
  68. [YEAR:16-1-1987]Bathurst,I.C., Brennan,S.O., Carrell,R.W., Cousens,L.S., Brake,A.J. and Barr,P.J.
    Yeast KEX2 protease has the properties of a human proalbumin converting enzyme
    Science235, 348-350. PubMed  Europe PubMed
  69. Davidow,L.S., O'Donnell,M.M., Kaczmarek,F.S., Pereira,D.A., DeZeeuw,J.R. and Franke,A.E.
    Cloning and sequencing of the alkaline extracellular protease gene of Yarrowia lipolytica
    J Bacteriol169, 4621-4629. PubMed  Europe PubMed
    70. 1985
  70. Achstetter,T. and Wolf,D.H.
    Hormone processing and membrane-bound proteinases in yeast
    EMBO J4, 173-177. PubMed  Europe PubMed
  71. Mizuno,K. and Matsuo,H.
    Pheromone-processing protease of the yeast Saccharomyces cerevisiae - a reply
    Nature314, 384-385. DOI
  72. Thorner,J.
    Pheromone-processing protease of the yeast Saccharomyces cerevisiae
    Nature314, 384-385. PubMed  Europe PubMed DOI
    73. 1984
  73. Julius,D., Brake,A., Blair,L., Kunisawa,R. and Thorner,J.
    Isolation of the putative structural gene for the lysine- arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor
    Cell37, 1075-1089. PubMed  Europe PubMed
  74. Mizuno,K. and Matsuo,H.
    A novel protease from yeast with specificity towards paired basic residues
    Nature309, 558-560. PubMed  Europe PubMed

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