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Literature for peptidase S01.268: alpha-lytic endopeptidase

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Konstantinov,M., Kaluzhskiy,L., Yablokov,E., Zhdanov,D., Ivanov,A. and Toropygin,I.<br>Substrate Specificity and Peptide Motif Preferences of beta-Lytic and L5 Proteases from Lysobacter spp. Revealed by LC-MS/MS Analysis<br>Int J Mol Sci (2025) 26 PubMed  Europe PubMed DOI  PMC  EPMC
  2. Kudryakova,I., Afoshin,A., Leontyevskaya,E. and Leontyevskaya,N.<br>Development of Efficient Expression Systems for Bacteriolytic Proteases L1 and L5 of Lysobacter capsici XL1<br>Int J Mol Sci (2025) 26 PubMed  Europe PubMed DOI  PMC  EPMC
    3. 2023
  3. Afoshin,A., Kudryakova,I., Tarlachkov,S., Leontyevskaya,E., Zelenov,D., Rudenko,P. and Leontyevskaya Vasilyeva,N.<br>Transcriptomic Analysis Followed by the Isolation of Extracellular Bacteriolytic Proteases from Lysobacter capsici VKM B-2533(T)<br>Int J Mol Sci (2023) 24 PubMed  Europe PubMed DOI  PMC  EPMC
    4. 2021
  4. Nixon,C.F., Lim,S.A., Sailer,Z.R., Zheludev,I.N., Gee,C.L., Kelch,B.A., Harms,M.J. and Marqusee,S.<br>Exploring the Evolutionary History of Kinetic Stability in the alpha-Lytic Protease Family<br>Biochemistry (2021) 60, 170-181. PubMed  Europe PubMed DOI
    5. 2018
  5. Kudryakova,I.V., Gabdulkhakov,A.G., Tishchenko,S.V., Lysanskaya,V.Y., Suzina,N.E., Tsfasman,I.M., Afoshin,A.S. and Vasilyeva,N.V.<br>Structural and functional properties of antimicrobial protein L5 of Lysobacter sp. XL1<br>Appl Microbiol Biotechnol (2018) 102, 10043-10053. PubMed  Europe PubMed DOI  S
    6. 2016
  6. Tishchenko,S., Gabdulkhakov,A., Melnik,B., Kudryakova,I., Latypov,O., Vasilyeva,N. and Leontievsky,A.<br>Structural studies of component of lysoamidase bacteriolytic complex from Lysobacter sp. XL1<br>Protein J (2016) 35, 44-50. PubMed  Europe PubMed DOI  S
    7. 2015
  7. Kudryakova,I.V., Suzina,N.E. and Vasilyeva,N.V.<br>Biogenesis of Lysobacter sp. XL1 vesicles<br>FEMS Microbiol Lett (2015) 362, fnv137- PubMed  Europe PubMed DOI
  8. Tsfasman,I.M., Lapteva,Y.S., Krasovskaya,L.A., Kudryakova,I.V., Vasilyeva,N.V., Granovsky,I.E. and Stepnaya,O.A.<br>Gene expression of lytic endopeptidases AlpA and AlpB from Lysobacter sp. XL1 in pseudomonads<br>J Mol Microbiol Biotechnol (2015) 25, 244-252. PubMed  Europe PubMed DOI  E
    9. 2014
  9. Gokcen,A., Vilcinskas,A. and Wiesner,J.<br>Biofilm-degrading enzymes from Lysobacter gummosus<br>Virulence (2014) 5, 378-387. PubMed  Europe PubMed DOI
  10. Meyer,J.G., Kim,S., Maltby,D.A., Ghassemian,M., Bandeira,N. and Komives,E.A.<br>Expanding proteome coverage with orthogonal-specificity alpha-lytic proteases<br>Mol Cell Proteomics (2014) 13, 823-835. PubMed  Europe PubMed DOI
  11. Vasilyeva,N.V., Shishkova,N.A., Marinin,L.I., Ledova,L.A., Tsfasman,I.M., Muranova,T.A., Stepnaya,O.A. and Kulaev,I.S.<br>Lytic peptidase L5 of Lysobacter sp. XL1 with broad antimicrobial spectrum<br>J Mol Microbiol Biotechnol (2014) 24, 59-66. PubMed  Europe PubMed DOI
    12. 2012
  12. Everill,P., Sudmeier,J.L. and Bachovchin,W.W.<br>Direct NMR observation and pKa determination of the Asp102 side chain in a serine protease<br>J Am Chem Soc (2012) 134, 2348-2354. PubMed  Europe PubMed DOI
  13. Jaswal,S.S., Fuhrmann,C.N., Ota,N., Rader,S.D. and Agard,D.A.<br>a-Lytic protease<br>[ISSN:978-0-12-407742-3] (2012) 3, 2558-2565. DOI
  14. Lapteva,Y.S., Zolova,O.E., Shlyapnikov,M.G., Tsfasman,I.M., Muranova,T.A., Stepnaya,O.A., Kulaev,I.S. and Granovsky,I.E.<br>Cloning and expression analysis of genes encoding lytic endopeptidases L1 and L5 from Lysobacter sp. strain XL1<br>Appl Environ Microbiol (2012) 78, 7082-7089. PubMed  Europe PubMed DOI
    15. 2010
  15. Salimi,N.L., Ho,B. and Agard,D.A.<br>Unfolding simulations reveal the mechanism of extreme unfolding cooperativity in the kinetically stable alpha-lytic protease<br>PLoS Comput Biol (2010) 6, e1000689-e1000689. PubMed  Europe PubMed DOI  PMC  EPMC
    16. 2009
  16. Deng,N.J. and Cieplak,P.<br>Insights into affinity and specificity in the complexes of alpha-lytic protease and its inhibitor proteins: binding free energy from molecular dynamics simulation<br>Phys Chem Chem Phys (2009) 11, 4968-4981. PubMed  Europe PubMed DOI
  17. Kang,H., Yao,X.Q., She,Z.S. and Zhu,H.<br>Water-protein interplay reveals the specificity of alpha-lytic protease<br>Biochem Biophys Res Commun (2009) 385, 165-169. PubMed  Europe PubMed DOI
    18. 2008
  18. Vasilyeva,N.V., Tsfasman,I.M., Suzina,N.E., Stepnaya,O.A. and Kulaev,I.S.<br>Secretion of bacteriolytic endopeptidase L5 of Lysobacter sp. XL1 into the medium by means of outer membrane vesicles<br>FEBS J (2008) 275, 3827-3835. PubMed  Europe PubMed DOI
    19. 2006
  19. [YEAR:19-7-2006]Fuhrmann,C.N., Daugherty,M.D. and Agard,D.A.<br>Subangstrom crystallography reveals that short ionic hydrogen bonds, and not a His-Asp low-barrier hydrogen bond, stabilize the transition state in serine protease catalysis<br>J Am Chem Soc (19-7-2006) 128, 9086-9102. PubMed  Europe PubMed DOI  S
  20. [YEAR:26-9-2006]Qasim,M.A., Van Etten,R.L., Yeh,T., Saunders,C., Ganz,P.J., Qasim,S., Wang,L. and Laskowski,M., Jr.<br>Despite having a common P1 Leu, eglin C inhibits alpha-lytic proteinase a million-fold more strongly than does turkey ovomucoid third domain<br>Biochemistry (26-9-2006) 45, 11342-11348. PubMed  Europe PubMed DOI
    21. 2005
  21. [YEAR:25-1-2005]Haddad,K.C., Sudmeier,J.L., Bachovchin,D.A. and Bachovchin,W.W.<br>alpha-Lytic protease can exist in two separately stable conformations with different His57 mobilities and catalytic activities<br>Proc Natl Acad Sci U S A (25-1-2005) 102, 1006-1011. PubMed  Europe PubMed DOI  PMC  EPMC
  22. [YEAR:25-7-2005]Truhlar,S.M. and Agard,D.A.<br>The folding landscape of an alpha-lytic protease variant reveals the role of a conserved beta-hairpin in the development of kinetic stability<br>Proteins (25-7-2005) 61, 105-114. PubMed  Europe PubMed DOI
    23. 2004
  23. Cunningham,E.L. and Agard,D.A.<br>Disabling the folding catalyst is the last critical step in alpha-lytic protease folding<br>Protein Sci (2004) 13, 325-331. PubMed  Europe PubMed DOI  PMC  EPMC
  24. Fuhrmann,C.N., Ota,N., Rader,S.D. and Agard,D.A.<br>alpha-Lytic protease<br>[ISSN:0-12-079610-4] (2004) 2, 1465-1471.  V
  25. [YEAR:14-5-2004]Fuhrmann,C.N., Kelch,B.A., Ota,N. and Agard,D.A.<br>The 0.83 A resolution crystal structure of alpha-lytic protease reveals the detailed structure of the active site and identifies a source of conformational strain<br>J Mol Biol (14-5-2004) 338, 999-1013. PubMed  Europe PubMed DOI  S
    26. 2003
  26. [YEAR:18-11-2003]Cunningham,E.L. and Agard,D.A.<br>Interdependent folding of the N- and C-terminal domains defines the cooperative folding of alpha-lytic protease<br>Biochemistry (18-11-2003) 42, 13212-13219. PubMed  Europe PubMed DOI
    27. 2002
  27. [YEAR:16-7-2002]Cunningham,E.L., Mau,T., Truhlar,S.M. and Agard,D.A.<br>The pro region N-terminal domain provides specific interactions required for catalysis of alpha-lytic protease folding<br>Biochemistry (16-7-2002) 41, 8860-8867. PubMed  Europe PubMed DOI
  28. [YEAR:17-1-2002]Jaswal,S.S., Sohl,J.L., Davis,J.H. and Agard,D.A.<br>Energetic landscape of alpha-lytic protease optimizes longevity through kinetic stability<br>Nature (17-1-2002) 415, 343-346. PubMed  Europe PubMed DOI
    29. 2001
  29. [YEAR:20-8-2001]Lien,S., Milner,S.J., Graham,L.D., Wallace,J.C. and Francis,G.L.<br>Linkers for improved cleavage of fusion proteins with an engineered alpha-lytic protease<br>Biotechnol Bioeng (20-8-2001) 74, 335-343. PubMed  Europe PubMed DOI
  30. Ota,N. and Agard,D.A.<br>Enzyme specificity under dynamic control II: Principal component analysis of alpha-lytic protease using global and local solvent boundary conditions<br>Protein Sci (2001) 10, 1403-1414. PubMed  Europe PubMed DOI  PMC  EPMC
    31. 1999
  31. [YEAR:13-4-1999]Anderson,D.E., Peters,R.J., Wilk,B. and Agard,D.A.<br>alpha-Lytic protease precursor: Characterization of a structured folding intermediate<br>Biochemistry (13-4-1999) 38, 4728-4735. PubMed  Europe PubMed DOI
  32. [YEAR:28-9-1999]Cunningham,E.L., Jaswal,S.S., Sohl,J.L. and Agard,D.A.<br>Kinetic stability as a mechanism for protease longevity<br>Proc Natl Acad Sci U S A (28-9-1999) 96, 11008-11014. PubMed  Europe PubMed DOI  PMC  EPMC
    33. 1998
  33. [YEAR:26-5-1998]Davis,J.H. and Agard,D.A.<br>Relationship between enzyme specificity and the backbone dynamics of free and inhibited alpha-lytic protease<br>Biochemistry (26-5-1998) 37, 7696-7707. PubMed  Europe PubMed DOI  I
  34. [YEAR:1-9-1998]Peters,R.J., Shiau,A.K., Sohl,J.L., Anderson,D.E., Tang,G., Silen,J.L. and Agard,D.A.<br>Pro region C-terminus: protease active site interactions are critical in catalyzing the folding of alpha-lytic protease<br>Biochemistry (1-9-1998) 37, 12058-12067. PubMed  Europe PubMed DOI
  35. Sauter,N.K., Mau,T., Rader,S.D. and Agard,D.A.<br>Structure of alpha-lytic protease complexed with its pro region<br>Nat Struct Biol (1998) 5, 945-950. PubMed  Europe PubMed DOI
  36. [YEAR:22-10-1998]Sohl,J.L., Jaswal,S.S. and Agard,D.A.<br>Unfolded conformations of alpha-lytic protease are more stable than its native state<br>Nature (22-10-1998) 395, 817-819. PubMed  Europe PubMed DOI
    37. 1997
  37. Li,S., Norioka,S. and Sakiyama,F.<br>Purification, staphylolytic activity, and cleavage sites of alpha-lytic protease from Achromobacter lyticus<br>J Biochem (1997) 122, 772-778. PubMed  Europe PubMed
  38. Rader,S.D. and Agard,D.A.<br>Conformational substates in enzyme mechanism: the 120 K structure of alpha-lytic protease at 1.5 A resolution<br>Protein Sci (1997) 6, 1375-1386. PubMed  Europe PubMed DOI  PMC  EPMC  S
  39. Unutmaz,C., Cail,R.G. and Haggett,K.D.<br>Increased alpha-lytic protease production from recombinant Escherichia coli grown with oxygen limitation<br>Biotechnol Lett (1997) 19, 731-734.  E
    40. 1995
  40. Graham,L.D., Haggett,K.D., Hayes,P.J., Schober,P.A., Jennings,P.A. and Whittaker,R.G.<br>Random mutagenesis of the substrate-binding site of a serine protease. A new library of alpha-lytic protease S1 mutants<br>Ann N Y Acad Sci (1995) 750, 10-14. PubMed  Europe PubMed DOI
  41. Mace,J.E. and Agard,D.A.<br>Kinetic and structural characterization of mutations of glycine 216 in alpha-lytic protease: a new target for engineering substrate specificity<br>J Mol Biol (1995) 254, 720-736. PubMed  Europe PubMed DOI
    42. 1994
  42. [YEAR:12-4-1994]Schellenberger,V., Turck,C.W. and Rutter,W.J.<br>Role of the S' subsites in serine protease catalysis. Active-site mapping of rat chymotrypsin, rat trypsin, alpha-lytic protease, and cercarial protease from Schistosoma mansoni<br>Biochemistry (12-4-1994) 33, 4251-4257. PubMed  Europe PubMed
    43. 1993
  43. Tsilikounas,E., Kettner,C.A. and Bachovchin,W.W.<br>11B NMR spectroscopy of peptide boronic acid inhibitor complexes of alpha-lytic protease. Direct evidence for tetrahedral boron in both boron-histidine and boron-serine adduct complexes<br>Biochemistry (1993) 32, 12651-12655. PubMed  Europe PubMed DOI  I
    44. 1991
  44. Bone,R., Sampson,N.S., Bartlett,P.A. and Agard,D.A.<br>Crystal structures of alpha-lytic protease complexes with irreversibly bound phosphonate esters<br>Biochemistry (1991) 30, 2263-2272. PubMed  Europe PubMed  S
    45. 1989
  45. [YEAR:19-9-1989]Bone,R., Frank,D., Kettner,C.A. and Agard,D.A.<br>Structural analysis of specificity: alpha-lytic protease complexes with analogues of reaction intermediates<br>Biochemistry (19-9-1989) 28, 7600-7609. PubMed  Europe PubMed  I
    46. 1988
  46. [YEAR:4-10-1988]Bachovchin,W.W., Wong,W.Y., Farr-Jones,S., Shenvi,A.B. and Kettner,C.A.<br>Nitrogen-15 NMR spectroscopy of the catalytic-triad histidine of a serine protease in peptide boronic acid inhibitor complexes<br>Biochemistry (4-10-1988) 27, 7689-7697. PubMed  Europe PubMed  I
  47. Epstein,D.M. and Wensink,P.C.<br>The alpha-lytic protease gene of Lysobacter enzymogenes. The nucleotide sequence predicts a large prepro-peptide with homology to pro-peptides of other chymotrypsin-like enzymes<br>J Biol Chem (1988) 263, 16586-16590. PubMed  Europe PubMed
    48. 1982
  48. Roberts,J.D., Yu,C., Flanagan,C. and Birdseye,T.R.<br>A nitrogen-15 nuclear magnetic resonance study of the acid-base and tautomeric equilibria of 4-substituted imidazoles and its relevance to the catalytic mechanism of alpha-lytic protease<br>J Am Chem Soc (1982) 104, 3945-3949.
    49. 1979
  49. [YEAR:15-7-1979]Brayer,G.D., Delbaere,L.T. and James,M.N.<br>Molecular structure of the alpha-lytic protease from Myxobacter 495 at 2.8 Angstroms resolution<br>J Mol Biol (15-7-1979) 131, 743-775. PubMed  Europe PubMed
    50. 1978
  50. Bachovchin,W.W. and Roberts,J.D.<br>Nitrogen-15 nuclear magnetic resonance spectroscopy. The state of histidine in the catalytic triad of alpha-lytic protease. Implications for the charge-relay mechanism of peptide-bond cleavage by serine proteases<br>J Am Chem Soc (1978) 100, 8041-8047.
    51. 1970
  51. Olson,M.O., Nagabhushan,N., Dzwiniel,M., Smillie,L.B. and Whitaker,D.R.<br>Primary structure of alpha-lytic protease: a bacterial homologue of the pancreatic serine proteases<br>Nature (1970) 228, 438-442. PubMed  Europe PubMed DOI
  52. Whitaker,D.R.<br>The alpha-lytic protease of a myxobacterium<br>Methods Enzymol (1970) 19, 599-613. DOI  V
    53. 1967
  53. Whitaker,D.R. and Roy,C.<br>Concerning the nature of the alpha- and beta-lytic proteases of Sorangium sp<br>Can J Biochem (1967) 45, 911-916. PubMed  Europe PubMed
    54. 1965
  54. Tsai,C.S., Whitaker,D.R., Jurasek,L. and Gillespie,D.C.<br>Lytic enzymes of Sorangium sp. Action of the alpha- and beta-lytic proteases on two bacterial mucopeptides<br>Can J Biochem (1965) 43, 1971-1983. PubMed  Europe PubMed
  55. Whitaker,D.R., Roy,C., Tsai,C.S. and Jurasek,L.<br>Lytic enzymes of Sorangium sp. A comparison of the proteolytic properties of the alpha- and beta-lytic proteases<br>Can J Biochem (1965) 43, 1961-1970. PubMed  Europe PubMed
  56. Whitaker,D.R., Cook,F.D. and Gillespie,D.C.<br>Lytic enzymes of Sorangium sp. Some aspects of enzyme production in submerged culture<br>Can J Biochem (1965) 43, 1927-1933. PubMed  Europe PubMed
  57. Whitaker,D.R.<br>Lytic enzymes of Sorangium sp. Isolation and enzymatic properties of the alpha- and beta-lytic proteases<br>Can J Biochem (1965) 43, 1935-1954. PubMed  Europe PubMed

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