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Literature for peptidase S01.268: alpha-lytic endopeptidase

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

(Topics flags: S Structure, I Inhibitor, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Konstantinov,M., Kaluzhskiy,L., Yablokov,E., Zhdanov,D., Ivanov,A. and Toropygin,I.
    Substrate Specificity and Peptide Motif Preferences of beta-Lytic and L5 Proteases from Lysobacter spp. Revealed by LC-MS/MS Analysis
    Int J Mol Sci (2025) 26 PubMed  Europe PubMed DOI  PMC  EPMC
  2. Kudryakova,I., Afoshin,A., Leontyevskaya,E. and Leontyevskaya,N.
    Development of Efficient Expression Systems for Bacteriolytic Proteases L1 and L5 of Lysobacter capsici XL1
    Int J Mol Sci (2025) 26 PubMed  Europe PubMed DOI  PMC  EPMC
    3. 2023
  3. Afoshin,A., Kudryakova,I., Tarlachkov,S., Leontyevskaya,E., Zelenov,D., Rudenko,P. and Leontyevskaya Vasilyeva,N.
    Transcriptomic Analysis Followed by the Isolation of Extracellular Bacteriolytic Proteases from Lysobacter capsici VKM B-2533(T)
    Int J Mol Sci (2023) 24 PubMed  Europe PubMed DOI  PMC  EPMC
    4. 2021
  4. Nixon,C.F., Lim,S.A., Sailer,Z.R., Zheludev,I.N., Gee,C.L., Kelch,B.A., Harms,M.J. and Marqusee,S.
    Exploring the Evolutionary History of Kinetic Stability in the alpha-Lytic Protease Family
    Biochemistry (2021) 60, 170-181. PubMed  Europe PubMed DOI
    5. 2018
  5. Kudryakova,I.V., Gabdulkhakov,A.G., Tishchenko,S.V., Lysanskaya,V.Y., Suzina,N.E., Tsfasman,I.M., Afoshin,A.S. and Vasilyeva,N.V.
    Structural and functional properties of antimicrobial protein L5 of Lysobacter sp. XL1
    Appl Microbiol Biotechnol (2018) 102, 10043-10053. PubMed  Europe PubMed DOI  S
    6. 2016
  6. Tishchenko,S., Gabdulkhakov,A., Melnik,B., Kudryakova,I., Latypov,O., Vasilyeva,N. and Leontievsky,A.
    Structural studies of component of lysoamidase bacteriolytic complex from Lysobacter sp. XL1
    Protein J (2016) 35, 44-50. PubMed  Europe PubMed DOI  S
    7. 2015
  7. Kudryakova,I.V., Suzina,N.E. and Vasilyeva,N.V.
    Biogenesis of Lysobacter sp. XL1 vesicles
    FEMS Microbiol Lett (2015) 362, fnv137- PubMed  Europe PubMed DOI
  8. Tsfasman,I.M., Lapteva,Y.S., Krasovskaya,L.A., Kudryakova,I.V., Vasilyeva,N.V., Granovsky,I.E. and Stepnaya,O.A.
    Gene expression of lytic endopeptidases AlpA and AlpB from Lysobacter sp. XL1 in pseudomonads
    J Mol Microbiol Biotechnol (2015) 25, 244-252. PubMed  Europe PubMed DOI  E
    9. 2014
  9. Gokcen,A., Vilcinskas,A. and Wiesner,J.
    Biofilm-degrading enzymes from Lysobacter gummosus
    Virulence (2014) 5, 378-387. PubMed  Europe PubMed DOI
  10. Meyer,J.G., Kim,S., Maltby,D.A., Ghassemian,M., Bandeira,N. and Komives,E.A.
    Expanding proteome coverage with orthogonal-specificity alpha-lytic proteases
    Mol Cell Proteomics (2014) 13, 823-835. PubMed  Europe PubMed DOI
  11. Vasilyeva,N.V., Shishkova,N.A., Marinin,L.I., Ledova,L.A., Tsfasman,I.M., Muranova,T.A., Stepnaya,O.A. and Kulaev,I.S.
    Lytic peptidase L5 of Lysobacter sp. XL1 with broad antimicrobial spectrum
    J Mol Microbiol Biotechnol (2014) 24, 59-66. PubMed  Europe PubMed DOI
    12. 2012
  12. Everill,P., Sudmeier,J.L. and Bachovchin,W.W.
    Direct NMR observation and pKa determination of the Asp102 side chain in a serine protease
    J Am Chem Soc (2012) 134, 2348-2354. PubMed  Europe PubMed DOI
  13. Jaswal,S.S., Fuhrmann,C.N., Ota,N., Rader,S.D. and Agard,D.A.
    a-Lytic protease
    [ISSN:978-0-12-407742-3] (2012) 3, 2558-2565. DOI
  14. Lapteva,Y.S., Zolova,O.E., Shlyapnikov,M.G., Tsfasman,I.M., Muranova,T.A., Stepnaya,O.A., Kulaev,I.S. and Granovsky,I.E.
    Cloning and expression analysis of genes encoding lytic endopeptidases L1 and L5 from Lysobacter sp. strain XL1
    Appl Environ Microbiol (2012) 78, 7082-7089. PubMed  Europe PubMed DOI
    15. 2010
  15. Salimi,N.L., Ho,B. and Agard,D.A.
    Unfolding simulations reveal the mechanism of extreme unfolding cooperativity in the kinetically stable alpha-lytic protease
    PLoS Comput Biol (2010) 6, e1000689-e1000689. PubMed  Europe PubMed DOI  PMC  EPMC
    16. 2009
  16. Deng,N.J. and Cieplak,P.
    Insights into affinity and specificity in the complexes of alpha-lytic protease and its inhibitor proteins: binding free energy from molecular dynamics simulation
    Phys Chem Chem Phys (2009) 11, 4968-4981. PubMed  Europe PubMed DOI
  17. Kang,H., Yao,X.Q., She,Z.S. and Zhu,H.
    Water-protein interplay reveals the specificity of alpha-lytic protease
    Biochem Biophys Res Commun (2009) 385, 165-169. PubMed  Europe PubMed DOI
    18. 2008
  18. Vasilyeva,N.V., Tsfasman,I.M., Suzina,N.E., Stepnaya,O.A. and Kulaev,I.S.
    Secretion of bacteriolytic endopeptidase L5 of Lysobacter sp. XL1 into the medium by means of outer membrane vesicles
    FEBS J (2008) 275, 3827-3835. PubMed  Europe PubMed DOI
    19. 2006
  19. [YEAR:19-7-2006]Fuhrmann,C.N., Daugherty,M.D. and Agard,D.A.
    Subangstrom crystallography reveals that short ionic hydrogen bonds, and not a His-Asp low-barrier hydrogen bond, stabilize the transition state in serine protease catalysis
    J Am Chem Soc (19-7-2006) 128, 9086-9102. PubMed  Europe PubMed DOI  S
  20. [YEAR:26-9-2006]Qasim,M.A., Van Etten,R.L., Yeh,T., Saunders,C., Ganz,P.J., Qasim,S., Wang,L. and Laskowski,M., Jr.
    Despite having a common P1 Leu, eglin C inhibits alpha-lytic proteinase a million-fold more strongly than does turkey ovomucoid third domain
    Biochemistry (26-9-2006) 45, 11342-11348. PubMed  Europe PubMed DOI
    21. 2005
  21. [YEAR:25-1-2005]Haddad,K.C., Sudmeier,J.L., Bachovchin,D.A. and Bachovchin,W.W.
    alpha-Lytic protease can exist in two separately stable conformations with different His57 mobilities and catalytic activities
    Proc Natl Acad Sci U S A (25-1-2005) 102, 1006-1011. PubMed  Europe PubMed DOI  PMC  EPMC
  22. [YEAR:25-7-2005]Truhlar,S.M. and Agard,D.A.
    The folding landscape of an alpha-lytic protease variant reveals the role of a conserved beta-hairpin in the development of kinetic stability
    Proteins (25-7-2005) 61, 105-114. PubMed  Europe PubMed DOI
    23. 2004
  23. Cunningham,E.L. and Agard,D.A.
    Disabling the folding catalyst is the last critical step in alpha-lytic protease folding
    Protein Sci (2004) 13, 325-331. PubMed  Europe PubMed DOI  PMC  EPMC
  24. Fuhrmann,C.N., Ota,N., Rader,S.D. and Agard,D.A.
    alpha-Lytic protease
    [ISSN:0-12-079610-4] (2004) 2, 1465-1471.  V
  25. [YEAR:14-5-2004]Fuhrmann,C.N., Kelch,B.A., Ota,N. and Agard,D.A.
    The 0.83 A resolution crystal structure of alpha-lytic protease reveals the detailed structure of the active site and identifies a source of conformational strain
    J Mol Biol (14-5-2004) 338, 999-1013. PubMed  Europe PubMed DOI  S
    26. 2003
  26. [YEAR:18-11-2003]Cunningham,E.L. and Agard,D.A.
    Interdependent folding of the N- and C-terminal domains defines the cooperative folding of alpha-lytic protease
    Biochemistry (18-11-2003) 42, 13212-13219. PubMed  Europe PubMed DOI
    27. 2002
  27. [YEAR:16-7-2002]Cunningham,E.L., Mau,T., Truhlar,S.M. and Agard,D.A.
    The pro region N-terminal domain provides specific interactions required for catalysis of alpha-lytic protease folding
    Biochemistry (16-7-2002) 41, 8860-8867. PubMed  Europe PubMed DOI
  28. [YEAR:17-1-2002]Jaswal,S.S., Sohl,J.L., Davis,J.H. and Agard,D.A.
    Energetic landscape of alpha-lytic protease optimizes longevity through kinetic stability
    Nature (17-1-2002) 415, 343-346. PubMed  Europe PubMed DOI
    29. 2001
  29. [YEAR:20-8-2001]Lien,S., Milner,S.J., Graham,L.D., Wallace,J.C. and Francis,G.L.
    Linkers for improved cleavage of fusion proteins with an engineered alpha-lytic protease
    Biotechnol Bioeng (20-8-2001) 74, 335-343. PubMed  Europe PubMed DOI
  30. Ota,N. and Agard,D.A.
    Enzyme specificity under dynamic control II: Principal component analysis of alpha-lytic protease using global and local solvent boundary conditions
    Protein Sci (2001) 10, 1403-1414. PubMed  Europe PubMed DOI  PMC  EPMC
    31. 1999
  31. [YEAR:13-4-1999]Anderson,D.E., Peters,R.J., Wilk,B. and Agard,D.A.
    alpha-Lytic protease precursor: Characterization of a structured folding intermediate
    Biochemistry (13-4-1999) 38, 4728-4735. PubMed  Europe PubMed DOI
  32. [YEAR:28-9-1999]Cunningham,E.L., Jaswal,S.S., Sohl,J.L. and Agard,D.A.
    Kinetic stability as a mechanism for protease longevity
    Proc Natl Acad Sci U S A (28-9-1999) 96, 11008-11014. PubMed  Europe PubMed DOI  PMC  EPMC
    33. 1998
  33. [YEAR:26-5-1998]Davis,J.H. and Agard,D.A.
    Relationship between enzyme specificity and the backbone dynamics of free and inhibited alpha-lytic protease
    Biochemistry (26-5-1998) 37, 7696-7707. PubMed  Europe PubMed DOI  I
  34. [YEAR:1-9-1998]Peters,R.J., Shiau,A.K., Sohl,J.L., Anderson,D.E., Tang,G., Silen,J.L. and Agard,D.A.
    Pro region C-terminus: protease active site interactions are critical in catalyzing the folding of alpha-lytic protease
    Biochemistry (1-9-1998) 37, 12058-12067. PubMed  Europe PubMed DOI
  35. Sauter,N.K., Mau,T., Rader,S.D. and Agard,D.A.
    Structure of alpha-lytic protease complexed with its pro region
    Nat Struct Biol (1998) 5, 945-950. PubMed  Europe PubMed DOI
  36. [YEAR:22-10-1998]Sohl,J.L., Jaswal,S.S. and Agard,D.A.
    Unfolded conformations of alpha-lytic protease are more stable than its native state
    Nature (22-10-1998) 395, 817-819. PubMed  Europe PubMed DOI
    37. 1997
  37. Li,S., Norioka,S. and Sakiyama,F.
    Purification, staphylolytic activity, and cleavage sites of alpha-lytic protease from Achromobacter lyticus
    J Biochem (1997) 122, 772-778. PubMed  Europe PubMed
  38. Rader,S.D. and Agard,D.A.
    Conformational substates in enzyme mechanism: the 120 K structure of alpha-lytic protease at 1.5 A resolution
    Protein Sci (1997) 6, 1375-1386. PubMed  Europe PubMed DOI  PMC  EPMC  S
  39. Unutmaz,C., Cail,R.G. and Haggett,K.D.
    Increased alpha-lytic protease production from recombinant Escherichia coli grown with oxygen limitation
    Biotechnol Lett (1997) 19, 731-734.  E
    40. 1995
  40. Graham,L.D., Haggett,K.D., Hayes,P.J., Schober,P.A., Jennings,P.A. and Whittaker,R.G.
    Random mutagenesis of the substrate-binding site of a serine protease. A new library of alpha-lytic protease S1 mutants
    Ann N Y Acad Sci (1995) 750, 10-14. PubMed  Europe PubMed DOI
  41. Mace,J.E. and Agard,D.A.
    Kinetic and structural characterization of mutations of glycine 216 in alpha-lytic protease: a new target for engineering substrate specificity
    J Mol Biol (1995) 254, 720-736. PubMed  Europe PubMed DOI
    42. 1994
  42. [YEAR:12-4-1994]Schellenberger,V., Turck,C.W. and Rutter,W.J.
    Role of the S' subsites in serine protease catalysis. Active-site mapping of rat chymotrypsin, rat trypsin, alpha-lytic protease, and cercarial protease from Schistosoma mansoni
    Biochemistry (12-4-1994) 33, 4251-4257. PubMed  Europe PubMed
    43. 1993
  43. Tsilikounas,E., Kettner,C.A. and Bachovchin,W.W.
    11B NMR spectroscopy of peptide boronic acid inhibitor complexes of alpha-lytic protease. Direct evidence for tetrahedral boron in both boron-histidine and boron-serine adduct complexes
    Biochemistry (1993) 32, 12651-12655. PubMed  Europe PubMed DOI  I
    44. 1991
  44. Bone,R., Sampson,N.S., Bartlett,P.A. and Agard,D.A.
    Crystal structures of alpha-lytic protease complexes with irreversibly bound phosphonate esters
    Biochemistry (1991) 30, 2263-2272. PubMed  Europe PubMed  S
    45. 1989
  45. [YEAR:19-9-1989]Bone,R., Frank,D., Kettner,C.A. and Agard,D.A.
    Structural analysis of specificity: alpha-lytic protease complexes with analogues of reaction intermediates
    Biochemistry (19-9-1989) 28, 7600-7609. PubMed  Europe PubMed  I
    46. 1988
  46. [YEAR:4-10-1988]Bachovchin,W.W., Wong,W.Y., Farr-Jones,S., Shenvi,A.B. and Kettner,C.A.
    Nitrogen-15 NMR spectroscopy of the catalytic-triad histidine of a serine protease in peptide boronic acid inhibitor complexes
    Biochemistry (4-10-1988) 27, 7689-7697. PubMed  Europe PubMed  I
  47. Epstein,D.M. and Wensink,P.C.
    The alpha-lytic protease gene of Lysobacter enzymogenes. The nucleotide sequence predicts a large prepro-peptide with homology to pro-peptides of other chymotrypsin-like enzymes
    J Biol Chem (1988) 263, 16586-16590. PubMed  Europe PubMed
    48. 1982
  48. Roberts,J.D., Yu,C., Flanagan,C. and Birdseye,T.R.
    A nitrogen-15 nuclear magnetic resonance study of the acid-base and tautomeric equilibria of 4-substituted imidazoles and its relevance to the catalytic mechanism of alpha-lytic protease
    J Am Chem Soc (1982) 104, 3945-3949.
    49. 1979
  49. [YEAR:15-7-1979]Brayer,G.D., Delbaere,L.T. and James,M.N.
    Molecular structure of the alpha-lytic protease from Myxobacter 495 at 2.8 Angstroms resolution
    J Mol Biol (15-7-1979) 131, 743-775. PubMed  Europe PubMed
    50. 1978
  50. Bachovchin,W.W. and Roberts,J.D.
    Nitrogen-15 nuclear magnetic resonance spectroscopy. The state of histidine in the catalytic triad of alpha-lytic protease. Implications for the charge-relay mechanism of peptide-bond cleavage by serine proteases
    J Am Chem Soc (1978) 100, 8041-8047.
    51. 1970
  51. Olson,M.O., Nagabhushan,N., Dzwiniel,M., Smillie,L.B. and Whitaker,D.R.
    Primary structure of alpha-lytic protease: a bacterial homologue of the pancreatic serine proteases
    Nature (1970) 228, 438-442. PubMed  Europe PubMed DOI
  52. Whitaker,D.R.
    The alpha-lytic protease of a myxobacterium
    Methods Enzymol (1970) 19, 599-613. DOI  V
    53. 1967
  53. Whitaker,D.R. and Roy,C.
    Concerning the nature of the alpha- and beta-lytic proteases of Sorangium sp
    Can J Biochem (1967) 45, 911-916. PubMed  Europe PubMed
    54. 1965
  54. Tsai,C.S., Whitaker,D.R., Jurasek,L. and Gillespie,D.C.
    Lytic enzymes of Sorangium sp. Action of the alpha- and beta-lytic proteases on two bacterial mucopeptides
    Can J Biochem (1965) 43, 1971-1983. PubMed  Europe PubMed
  55. Whitaker,D.R., Roy,C., Tsai,C.S. and Jurasek,L.
    Lytic enzymes of Sorangium sp. A comparison of the proteolytic properties of the alpha- and beta-lytic proteases
    Can J Biochem (1965) 43, 1961-1970. PubMed  Europe PubMed
  56. Whitaker,D.R., Cook,F.D. and Gillespie,D.C.
    Lytic enzymes of Sorangium sp. Some aspects of enzyme production in submerged culture
    Can J Biochem (1965) 43, 1927-1933. PubMed  Europe PubMed
  57. Whitaker,D.R.
    Lytic enzymes of Sorangium sp. Isolation and enzymatic properties of the alpha- and beta-lytic proteases
    Can J Biochem (1965) 43, 1935-1954. PubMed  Europe PubMed

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